FastQCFastQC Report
Sat 18 Jun 2016
SRR578610_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR578610_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10000641
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2883032.8828452096220634No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT273090.273072496053003No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT194040.1940275628332224No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT104180.10417332249002839No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCACG7500.030.399093
AAGCAGT121700.030.3324871
CGCGTAG4650.024.51600854-55
AGTGGTA150100.023.7328745
AGCAGTG165700.022.44492
GGTATCA161400.022.1301368
GTATCAA161400.022.1007089
CTCGCGT5350.021.75315352-53
TGGTATC168300.021.222847
GTGTACG11000.020.2943389
GTGGTAT176350.020.2809946
ATCAACG146650.019.8700510-11
GCAGTGG186250.019.866523
CAACGCA151050.019.3540912-13
ACGTACG6400.019.29581520-21
ACTCGCG5950.019.161150-51
CGCTAGT6250.018.23954490-91
ACGTGTA13350.018.1450717
CAGTGGT206050.017.6348424
CGTACGC6900.017.209220-21
Produced by FastQC (version 0.11.5)