##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR578610_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 10000641 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.478591522283423 31.0 31.0 34.0 30.0 34.0 2 31.79307626381149 31.0 31.0 34.0 30.0 34.0 3 31.87168002531038 31.0 31.0 34.0 30.0 34.0 4 35.53751444532406 37.0 35.0 37.0 33.0 37.0 5 35.35143277315924 37.0 35.0 37.0 33.0 37.0 6 35.34486809395518 37.0 35.0 37.0 32.0 37.0 7 35.313898479107486 37.0 35.0 37.0 32.0 37.0 8 35.30066102762813 37.0 35.0 37.0 32.0 37.0 9 36.86526973620991 39.0 37.0 39.0 32.0 39.0 10-11 36.83288696194574 39.0 37.0 39.0 32.0 39.0 12-13 36.81733300895412 39.0 37.0 39.0 32.0 39.0 14-15 37.992643971521424 40.0 37.0 41.0 33.0 41.0 16-17 37.92623402839878 40.0 37.0 41.0 33.0 41.0 18-19 37.797479231581256 40.0 37.0 41.0 32.0 41.0 20-21 37.709280335130515 40.0 36.0 41.0 32.0 41.0 22-23 37.60638038101757 39.0 36.0 41.0 32.0 41.0 24-25 37.51702390876745 39.0 36.0 41.0 32.0 41.0 26-27 37.3283398034186 39.0 36.0 41.0 31.5 41.0 28-29 37.149279931156414 39.0 36.0 41.0 31.0 41.0 30-31 37.008938627034006 39.0 36.0 41.0 31.0 41.0 32-33 36.87004777993731 39.0 35.0 40.0 30.5 41.0 34-35 36.70125369963785 39.0 35.0 40.0 30.0 41.0 36-37 36.534547335515796 38.0 35.0 40.0 30.0 41.0 38-39 36.34242125079783 38.0 35.0 40.0 30.0 41.0 40-41 36.15620288739491 38.0 35.0 40.0 30.0 41.0 42-43 36.06855070589975 38.0 35.0 40.0 30.0 41.0 44-45 36.07839242504556 38.0 35.0 40.0 29.5 41.0 46-47 35.92793706923386 38.0 35.0 40.0 29.0 41.0 48-49 35.75760783733762 38.0 34.0 40.0 28.0 41.0 50-51 35.52513078911642 38.0 34.0 40.0 28.0 41.0 52-53 35.55101293007118 38.0 34.0 40.0 28.0 41.0 54-55 35.50971307739174 38.0 34.0 40.0 28.0 41.0 56-57 35.317182668590945 38.0 34.0 40.0 28.0 41.0 58-59 35.085810249562996 37.0 34.0 40.0 27.0 41.0 60-61 34.80348014692258 37.0 33.0 40.0 27.0 41.0 62-63 34.5024380437214 36.5 33.0 39.5 26.0 41.0 64-65 34.16261782619734 36.0 33.0 39.0 26.0 41.0 66-67 33.80813659844404 35.5 32.5 39.0 26.0 40.5 68-69 33.431838569147715 35.0 32.0 38.5 26.0 40.0 70-71 33.044012078825745 35.0 32.0 38.0 25.0 40.0 72-73 32.65577576477348 35.0 31.5 37.0 24.5 39.5 74-75 32.26017537275861 35.0 31.0 37.0 24.0 39.0 76-77 30.939148700568296 33.5 29.5 35.5 22.0 38.0 78-79 31.59432095402685 34.0 31.0 36.0 23.0 38.0 80-81 31.49600020638677 34.0 31.0 35.5 23.0 37.0 82-83 31.212475580315303 34.0 31.0 35.0 22.5 37.0 84-85 30.911326833949943 34.0 31.0 35.0 20.5 36.5 86-87 30.586953326291784 34.0 30.0 35.0 20.0 36.0 88-89 30.278786079812285 34.0 30.0 35.0 19.5 36.0 90-91 29.96489515022087 34.0 30.0 35.0 18.0 35.5 92-93 29.605455590296664 34.0 29.5 35.0 14.0 35.0 94-95 29.23227671106282 34.0 29.0 35.0 6.0 35.0 96-97 28.83760215970156 34.0 29.0 35.0 2.0 35.0 98-99 28.350168654189268 34.0 29.0 35.0 2.0 35.0 100-101 27.22103048194611 33.0 27.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.37911716599344913 1101 2 0.36344512950289243 1101 3 0.33306600505886763 1101 4 0.2929399056694919 1101 5 0.3360019212706362 1101 6 0.2802042292020914 1101 7 0.3133677838785971 1101 8 0.3071465813206302 1101 9 0.3305891077177492 1101 10-11 0.3443001629948057 1101 12-13 0.3674006435584758 1101 14-15 0.4809499618831623 1101 16-17 0.4764667911412488 1101 18-19 0.3992565730052533 1101 20-21 0.39856492170772384 1101 22-23 0.4813919568597669 1101 24-25 0.5026140422243657 1101 26-27 0.47280051073867213 1101 28-29 0.5310129390577103 1101 30-31 0.5336727633726568 1101 32-33 0.47741070831512644 1101 34-35 0.512916935811873 1101 36-37 0.5049052078942751 1101 38-39 0.4227585892335384 1101 40-41 0.5187904158536369 1101 42-43 0.4514197246250049 1101 44-45 0.3972167545455889 1101 46-47 0.3977246290079606 1101 48-49 0.3798212332220885 1101 50-51 0.3378541367054737 1101 52-53 0.4692445866446704 1101 54-55 0.47613199634626113 1101 56-57 0.5009538692070663 1101 58-59 0.43198356609215693 1101 60-61 0.4931146738225891 1101 62-63 0.33469988158356045 1101 64-65 0.4137394407307937 1101 66-67 0.3838380512880022 1101 68-69 0.4149245101694774 1101 70-71 0.3623837393296512 1101 72-73 0.43043506592029246 1101 74-75 0.3963543529690696 1101 76-77 0.29216556077992806 1101 78-79 0.4138001091671697 1101 80-81 0.43126537729775904 1101 82-83 0.4093000205390851 1101 84-85 0.4838319570189391 1101 86-87 0.4195470571654454 1101 88-89 0.35620269199118937 1101 90-91 0.4784057798886714 1101 92-93 0.411310577101176 1101 94-95 0.47107300573743416 1101 96-97 0.4083136380496839 1101 98-99 0.48594292749720935 1101 100-101 0.6468759308865089 1102 1 0.2877645872152854 1102 2 0.2939885074755324 1102 3 0.37299004098012034 1102 4 0.24933380136989314 1102 5 0.3289004789560366 1102 6 0.2146327252797775 1102 7 0.20943151631949775 1102 8 0.21182169142128515 1102 9 0.4120097211833098 1102 10-11 0.3515123658247248 1102 12-13 0.23248308100712478 1102 14-15 0.46948054657877947 1102 16-17 0.42898741979443145 1102 18-19 0.4461852956472754 1102 20-21 0.4486543863769228 1102 22-23 0.35139265061540215 1102 24-25 0.36795980873563394 1102 26-27 0.37798283169118463 1102 28-29 0.39913821853714637 1102 30-31 0.3725113770133177 1102 32-33 0.4696402413339342 1102 34-35 0.49476126906631634 1102 36-37 0.4768915091291248 1102 38-39 0.48003326612874275 1102 40-41 0.4134465407038803 1102 42-43 0.4218077436430434 1102 44-45 0.44530767893819956 1102 46-47 0.40375947611801877 1102 48-49 0.3831984481619628 1102 50-51 0.4262655954331933 1102 52-53 0.4759353140837561 1102 54-55 0.4381716446491737 1102 56-57 0.513700506427341 1102 58-59 0.622803448234464 1102 60-61 0.5093624741051883 1102 62-63 0.4819570011245773 1102 64-65 0.49287380004361836 1102 66-67 0.41878061885021367 1102 68-69 0.3834026194387192 1102 70-71 0.4025266664524736 1102 72-73 0.5187611489543258 1102 74-75 0.3993574398691493 1102 76-77 0.37106902879368064 1102 78-79 0.37951099883737527 1102 80-81 0.37674369873219504 1102 82-83 0.33300843913152534 1102 84-85 0.3125336421432081 1102 86-87 0.35839799302160813 1102 88-89 0.47211727322023833 1102 90-91 0.5329199007005236 1102 92-93 0.41445585265654117 1102 94-95 0.27493975350091304 1102 96-97 0.25672997374010365 1102 98-99 0.4019870626634976 1102 100-101 0.5011041839910639 1103 1 0.12114999646258084 1103 2 0.23676589979014295 1103 3 0.24842935092776486 1103 4 0.09783009088497607 1103 5 0.1932269192345828 1103 6 0.12111889842206125 1103 7 0.12348603631037491 1103 8 0.08205581459051814 1103 9 0.3237656001918552 1103 10-11 0.24011314960851138 1103 12-13 0.21810730405114498 1103 14-15 0.3320758936661221 1103 16-17 0.2963879179327833 1103 18-19 0.2859453617041581 1103 20-21 0.29066156795691 1103 22-23 0.296193586921504 1103 24-25 0.29429303252848626 1103 26-27 0.22644099497384929 1103 28-29 0.2938375191728255 1103 30-31 0.32083930850267706 1103 32-33 0.27266913003798265 1103 34-35 0.26710962219414114 1103 36-37 0.29386400988867933 1103 38-39 0.35727146211247174 1103 40-41 0.325125457450703 1103 42-43 0.3044563301318064 1103 44-45 0.13776649558163712 1103 46-47 0.26192563273043135 1103 48-49 0.22801781600990978 1103 50-51 0.28104063226513176 1103 52-53 0.29490958690192315 1103 54-55 0.17280433215136526 1103 56-57 0.18817280938533543 1103 58-59 0.24813916405172876 1103 60-61 0.2538003391671282 1103 62-63 0.1940899966641254 1103 64-65 0.16391281549218917 1103 66-67 0.11301534728787033 1103 68-69 0.11317874631587443 1103 70-71 0.10173904106866871 1103 72-73 0.10488196327850119 1103 74-75 0.09856023947028092 1103 76-77 0.055944650796654116 1103 78-79 0.10459703418636224 1103 80-81 0.16361920386494333 1103 82-83 0.10167808885719509 1103 84-85 0.17054129676659002 1103 86-87 0.18954660052905226 1103 88-89 0.20214419016649643 1103 90-91 0.2203627711051972 1103 92-93 0.19146687597591594 1103 94-95 0.2173090705520231 1103 96-97 0.22320305127480466 1103 98-99 0.37545643421832153 1103 100-101 0.5439850849707213 1104 1 0.16493521832502367 1104 2 0.16691644665006677 1104 3 0.19834613080622887 1104 4 0.14478329019149783 1104 5 0.13166457852823754 1104 6 0.1729830232003593 1104 7 0.15347678321447233 1104 8 0.09998280692506256 1104 9 0.2213461325679731 1104 10-11 0.22251413821311417 1104 12-13 0.19328052681265717 1104 14-15 0.23588352097855392 1104 16-17 0.2252436087820726 1104 18-19 0.2730555621958288 1104 20-21 0.35057368689718515 1104 22-23 0.33447399368409947 1104 24-25 0.28232323547587157 1104 26-27 0.281638552435048 1104 28-29 0.2342147452293517 1104 30-31 0.18732482779394388 1104 32-33 0.2265080100066399 1104 34-35 0.26720370708231655 1104 36-37 0.3101231607691659 1104 38-39 0.3950521065922601 1104 40-41 0.335356637097739 1104 42-43 0.24840746576952455 1104 44-45 0.3004900883608457 1104 46-47 0.22950863356114581 1104 48-49 0.14504517679547746 1104 50-51 0.24881825634827237 1104 52-53 0.29097886802459527 1104 54-55 0.31638831594838734 1104 56-57 0.2593141040834013 1104 58-59 0.35094172190395057 1104 60-61 0.41475359910236165 1104 62-63 0.2784418116490812 1104 64-65 0.34644301797254684 1104 66-67 0.41825788802480446 1104 68-69 0.352097682079922 1104 70-71 0.21270061834577092 1104 72-73 0.2759187795953437 1104 74-75 0.289943464797048 1104 76-77 0.2561548727800975 1104 78-79 0.24493198997453192 1104 80-81 0.27516800500863425 1104 82-83 0.30821517991378755 1104 84-85 0.3243995966034099 1104 86-87 0.338274514250152 1104 88-89 0.31542778757862067 1104 90-91 0.41674158132027017 1104 92-93 0.33903910799298487 1104 94-95 0.342112887001921 1104 96-97 0.39198416541480796 1104 98-99 0.4980106198537513 1104 100-101 0.5498336750432884 1105 1 0.14380715064977068 1105 2 0.1295555443707741 1105 3 0.09976906818118891 1105 4 0.13836708262368091 1105 5 0.13870268683731268 1105 6 0.17093609883564653 1105 7 0.1955233002640071 1105 8 0.13784772034823334 1105 9 0.14226241290612052 1105 10-11 0.11389878464945724 1105 12-13 0.15519527408931566 1105 14-15 0.19803541344602849 1105 16-17 0.10524392006358596 1105 18-19 0.16857776923480117 1105 20-21 0.1778628603106327 1105 22-23 0.1741817682215725 1105 24-25 0.16153747812875707 1105 26-27 0.23558097429469171 1105 28-29 0.16152980633738423 1105 30-31 0.14618793992367785 1105 32-33 0.2146901987760259 1105 34-35 0.22442894856972373 1105 36-37 0.28369602192154986 1105 38-39 0.3372052789566169 1105 40-41 0.41541977591820256 1105 42-43 0.2895528393727531 1105 44-45 0.23964465106080723 1105 46-47 0.21899046407531841 1105 48-49 0.24839573326914888 1105 50-51 0.3377564369971964 1105 52-53 0.20414473521103815 1105 54-55 0.3040907503520103 1105 56-57 0.27799136789897005 1105 58-59 0.17533858498098454 1105 60-61 0.11945696354231927 1105 62-63 0.15572192809486296 1105 64-65 0.22679327260248527 1105 66-67 0.2054850054800923 1105 68-69 0.14004575568608146 1105 70-71 0.06548805330159979 1105 72-73 0.10223201093099021 1105 74-75 0.19019247345647017 1105 76-77 0.08197257870482844 1105 78-79 0.13895603051133776 1105 80-81 0.09951730658727698 1105 82-83 0.23918452878404395 1105 84-85 0.3653199120110706 1105 86-87 0.32725543815688596 1105 88-89 0.27251671921904475 1105 90-91 0.28028375143926354 1105 92-93 0.27147855835591983 1105 94-95 0.28493374167169705 1105 96-97 0.3907288564505116 1105 98-99 0.5616683532374829 1105 100-101 0.6563681613103896 1106 1 -0.07126716787616161 1106 2 0.0013819000346195764 1106 3 -0.0686057867781571 1106 4 0.044276239433685305 1106 5 -0.006076905326899862 1106 6 0.06059391784649648 1106 7 0.035758912442304336 1106 8 0.09519491565025362 1106 9 -0.0639281779025822 1106 10-11 -0.06592680997616895 1106 12-13 -0.050169055364222004 1106 14-15 0.15542915313248074 1106 16-17 0.0830629872146531 1106 18-19 -0.013548262823583457 1106 20-21 0.011221333241927312 1106 22-23 0.05169205185610792 1106 24-25 0.1300134545168703 1106 26-27 0.2258549245944792 1106 28-29 0.11344189844647445 1106 30-31 0.09109444529903499 1106 32-33 0.059183510132157835 1106 34-35 0.03626648520817355 1106 36-37 0.05921014734458652 1106 38-39 -0.014333444610556967 1106 40-41 -0.041795063482560124 1106 42-43 -0.049687408006896305 1106 44-45 -0.04086841632204141 1106 46-47 0.036900161400751585 1106 48-49 0.07226731111909146 1106 50-51 0.039649292011915804 1106 52-53 -0.1027617288749525 1106 54-55 -0.12929532546372258 1106 56-57 -0.07085905149315153 1106 58-59 0.05166985056126805 1106 60-61 0.1459918889563525 1106 62-63 0.019764875611848254 1106 64-65 0.0015273197727836418 1106 66-67 -0.03471587201293147 1106 68-69 0.003990467338461201 1106 70-71 -0.07336150276726272 1106 72-73 -0.09280365041919936 1106 74-75 -0.16384186897975894 1106 76-77 -0.16518759919606651 1106 78-79 -0.12444783727070785 1106 80-81 0.024235049147097953 1106 82-83 0.002340538335026565 1106 84-85 0.16098894296355226 1106 86-87 0.2693507753198112 1106 88-89 0.24446179121504485 1106 90-91 0.3246016017268083 1106 92-93 0.23174319977882618 1106 94-95 0.349374643675592 1106 96-97 0.2840508539740867 1106 98-99 0.3981880156593185 1106 100-101 0.3033495421490251 1107 1 0.08538477947481127 1107 2 0.07386795817457426 1107 3 0.05204738180130164 1107 4 0.1146238869238303 1107 5 0.003429074620378003 1107 6 -0.07822953848319969 1107 7 0.038989314865617075 1107 8 -0.005692600877630127 1107 9 -0.11921968868507804 1107 10-11 -0.07824089761058417 1107 12-13 -0.1078998757154821 1107 14-15 -0.009160199586510487 1107 16-17 0.0682671875228138 1107 18-19 -0.05133261660436261 1107 20-21 -0.13766927915077787 1107 22-23 -0.14940509355681542 1107 24-25 -0.11677010160017431 1107 26-27 -0.06568568919016826 1107 28-29 -0.03912050257891764 1107 30-31 -0.03325258513481799 1107 32-33 -0.006547364516379162 1107 34-35 -0.05094803940424697 1107 36-37 -0.026283751919706333 1107 38-39 0.017036036134960852 1107 40-41 -0.07663310001036194 1107 42-43 -0.05001634364461438 1107 44-45 0.003993793995682893 1107 46-47 0.029071497824581627 1107 48-49 -0.054759533590484466 1107 50-51 -0.01904336310860799 1107 52-53 -0.04751297029235957 1107 54-55 -0.06407983316346844 1107 56-57 -0.08172780095104315 1107 58-59 -0.07468655167384952 1107 60-61 0.04618486974547409 1107 62-63 0.023973411189096794 1107 64-65 0.03401001884417809 1107 66-67 -0.014662237351210194 1107 68-69 0.105901041111089 1107 70-71 0.05122380526507442 1107 72-73 -0.033392444520487174 1107 74-75 0.014267743869183391 1107 76-77 0.03959184459279541 1107 78-79 0.01869341639349642 1107 80-81 0.13076422714391 1107 82-83 0.26997651561575964 1107 84-85 0.22202493952442026 1107 86-87 0.30254580399038034 1107 88-89 0.32446090313253606 1107 90-91 0.3233792504846562 1107 92-93 0.3423247166651642 1107 94-95 0.22922983561069898 1107 96-97 0.29028550147780763 1107 98-99 0.3506457747970799 1107 100-101 0.5324118719667652 1108 1 0.0388845605372552 1108 2 0.04607979199516876 1108 3 -0.01172258363179779 1108 4 0.04412474146635503 1108 5 -0.023300671638303072 1108 6 -0.03166983537918355 1108 7 0.056466155995352096 1108 8 -0.03138126553278653 1108 9 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-1.0641643099331368 2316 100-101 -1.3999442171678709 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 204.0 3 129.0 4 417.0 5 1095.0 6 2036.0 7 3167.0 8 5048.0 9 7711.0 10 10582.0 11 13633.0 12 16929.0 13 20193.0 14 23085.0 15 25713.0 16 30258.0 17 35766.0 18 42324.0 19 48951.0 20 52215.0 21 53355.0 22 58948.0 23 69976.0 24 86145.0 25 108072.0 26 136562.0 27 172888.0 28 214463.0 29 264654.0 30 321554.0 31 389174.0 32 473365.0 33 587767.0 34 764974.0 35 1115742.0 36 1324419.0 37 1761215.0 38 1471763.0 39 285794.0 40 355.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 17.241709765149775 30.203479048214056 28.42008456238321 24.13472662425296 2 22.72823312025699 30.135918287637764 25.986224283023457 21.149624309081787 3 22.430532202885793 28.633004624403576 26.47883270682349 22.45763046588714 4 22.352659302827544 28.094331885144747 26.94381743604123 22.60919137598648 5 22.930300423794318 28.49231336581003 27.473465108014455 21.103921102381197 6 23.18876563317457 28.531143555620265 25.849345154575836 22.43074565662933 7 23.29597672789174 28.54359035585819 26.869917638279382 21.29051527797068 8 22.513921885450912 28.96282140879051 26.376971990039415 22.14628471571915 9 21.938386656995274 28.646116753091256 27.2083767556151 22.20711983429837 10-11 21.88823870184533 29.080418111805294 26.511534052391617 22.519809133957757 12-13 21.904518110840907 29.235248944067582 26.235885903302187 22.624347041789324 14-15 22.1850101593498 29.346246840202227 26.02073467298093 22.448008327467043 16-17 22.41348665752825 28.9731221688373 26.082052052771225 22.53133912086322 18-19 22.273983919825916 29.191862559168726 26.082855913792702 22.45129760721266 20-21 22.42568933258078 29.000237834933156 26.257521586007528 22.316551246478536 22-23 22.27594775326542 28.922027373289172 26.346758327604526 22.455266545840885 24-25 22.23029503808806 28.910241853497194 26.28619005521746 22.57327305319729 26-27 22.499297795011337 28.69496065302214 26.566027117661754 22.239714434304762 28-29 22.489829277460952 28.81606219663207 26.431558634791685 22.262549891115295 30-31 22.380139421112595 28.835140544057126 26.41196024916369 22.372759785666588 32-33 22.403507866721704 28.91298209068889 26.311545258048312 22.371964784541102 34-35 22.399826210774933 28.92696152838524 26.30420913903338 22.369003121806447 36-37 22.41390602756051 28.911292737755172 26.295323096694172 22.37947813799014 38-39 22.431542720784055 28.89478503439876 26.292921948857195 22.380750295959995 40-41 22.4110579231905 28.94008086668317 26.268472408265502 22.380388801860825 42-43 22.404653125465597 28.967840897387052 26.23146734342674 22.39603863372061 44-45 22.386855817478395 28.988180349132346 26.253457108012064 22.371506725377195 46-47 22.392847991059988 29.038591298239126 26.204207755259695 22.364352955441195 48-49 22.43260562259149 29.04144763210674 26.172776567548777 22.353170177752997 50-51 22.47886529287987 29.01419433910452 26.153541304704785 22.353399063310817 52-53 22.384300628609676 29.104125959142525 26.128927835441253 22.382645576806553 54-55 22.410775476751528 29.11971883625963 26.03220061216073 22.437305074828114 56-57 22.43929776297828 29.199424776843042 25.994940024091456 22.366337436087218 58-59 22.425932074609868 29.271857700628896 25.96209770490062 22.340112519860615 60-61 22.422810469904572 29.28232657257532 25.91610515062176 22.378757806898346 62-63 22.403780633388383 29.34197646098641 25.931487844189316 22.322755061435892 64-65 22.39300201812942 29.3575258878282 25.962588735323067 22.28688335871932 66-67 22.335219658957474 29.398626338436074 25.95887774808058 22.307276254525867 68-69 22.34879405473145 29.449268203297667 25.946888460968303 22.255049281002584 70-71 22.34137106818433 29.498006427967994 25.892287663342188 22.26833484050549 72-73 22.312702776902672 29.48945331364539 25.881826254702517 22.316017654749423 74-75 22.387231614820564 29.537365124637077 25.857026928100286 22.21837633244207 76-77 22.294580917363195 29.653834189228473 25.86961675756584 22.181968135842492 78-79 22.34299281416061 29.67031313292818 25.77548279155306 22.211211261358148 80-81 22.328921045082055 29.684061935691858 25.780713236994746 22.20630378223134 82-83 22.34417572343486 29.79555575732074 25.706150936181583 22.154117583062817 84-85 22.297789158398686 29.790915088715447 25.72778944038103 22.183506312504836 86-87 22.275050373236937 29.8765693200738 25.67610633627823 22.172273970411034 88-89 22.28654416049796 29.90501652121359 25.67057808048337 22.137861237805083 90-91 22.25122444203336 29.930677489510476 25.66553294857881 22.15256511987735 92-93 22.26453756263639 29.998227102118918 25.636823318976827 22.10041201626786 94-95 22.192802910102046 30.06062679267163 25.61827344257899 22.12829685464734 96-97 22.19957567301974 30.08640281264741 25.587771863376414 22.126249650956435 98-99 22.187582775944062 30.1432528174944 25.550117237485075 22.11904716907646 100-101 22.293640436376116 30.111440878927837 25.46932251029159 22.125596174404453 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 302301.0 1 165991.5 2 21843.5 3 12732.0 4 10729.5 5 9398.5 6 8998.5 7 9019.0 8 8453.5 9 7755.0 10 7803.0 11 9857.0 12 20645.0 13 19879.0 14 9644.5 15 9445.0 16 9941.5 17 11228.5 18 13308.5 19 14566.0 20 16694.0 21 20717.5 22 25708.0 23 31865.0 24 39102.5 25 46748.5 26 55795.0 27 66085.0 28 74970.0 29 84117.0 30 96039.0 31 110969.0 32 125334.0 33 139015.0 34 154786.5 35 169159.5 36 184845.0 37 203667.5 38 220738.5 39 231165.5 40 246026.5 41 266158.5 42 281029.5 43 296983.5 44 309933.5 45 323843.0 46 337674.5 47 347444.5 48 361737.5 49 373792.5 50 375833.0 51 375364.5 52 378016.5 53 373687.0 54 356006.5 55 332276.5 56 302322.0 57 271281.5 58 251412.5 59 229743.5 60 197956.0 61 164084.5 62 131279.5 63 101914.5 64 79432.0 65 61183.0 66 47676.0 67 40219.0 68 32351.5 69 25248.5 70 20077.0 71 16558.0 72 14358.5 73 11570.5 74 9102.5 75 7067.0 76 5265.5 77 3810.0 78 2703.5 79 1881.0 80 1357.5 81 1072.0 82 921.5 83 808.0 84 684.0 85 541.5 86 391.0 87 260.5 88 146.5 89 86.5 90 57.0 91 34.5 92 23.0 93 14.0 94 10.5 95 9.0 96 3.5 97 2.5 98 1.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.14403076762779507 2 0.0 3 0.0 4 0.003319787201640375 5 3.499775664379913E-4 6 0.0011299275716426577 7 0.0 8 1.9998718082170933E-4 9 1.4999038561628198E-4 10-11 1.4499070609573928E-4 12-13 9.999359041085466E-6 14-15 9.999359041085466E-6 16-17 1.4999038561628198E-5 18-19 2.9998077123256396E-5 20-21 7.499519280814099E-5 22-23 1.5998974465736746E-4 24-25 0.0 26-27 0.0 28-29 0.006139606451226476 30-31 0.0014699057790395636 32-33 3.299788483558204E-4 34-35 2.099865398627948E-4 36-37 2.7998205315039307E-4 38-39 0.0013399141115054526 40-41 0.0035447727800647978 42-43 5.099673110953587E-4 44-45 4.949682725337305E-4 46-47 0.006534581133349352 48-49 0.00696955325163657 50-51 0.0073895263313621595 52-53 0.009539388525195534 54-55 0.004894686250611336 56-57 4.999679520542733E-6 58-59 6.499583376705553E-5 60-61 0.001104929174039944 62-63 9.449394293825767E-4 64-65 4.349721182872178E-4 66-67 6.999551328759826E-4 68-69 8.349464799306364E-4 70-71 0.0012449202006151404 72-73 0.00272482533869579 74-75 6.54958017191098E-4 76-77 0.0 78-79 0.0 80-81 9.999359041085466E-5 82-83 1.6998910369845293E-4 84-85 1.4999038561628198E-4 86-87 0.002909813480955871 88-89 0.001649894241779102 90-91 6.54958017191098E-4 92-93 6.499583376705553E-4 94-95 2.9498109171202126E-4 96-97 9.849368655469185E-4 98-99 0.0 100-101 1.9998718082170932E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.0000641E7 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.08546703013218 #Duplication Level Percentage of deduplicated Percentage of total 1 90.20412361992794 60.51385761086643 2 5.4094115589978085 7.2578580158712676 3 1.4728251303330089 2.9641548516631557 4 0.7627506397583985 2.0467793158289704 5 0.46225460022780035 1.5505282871554513 6 0.3083729036846797 1.2412404157874821 7 0.22869559477561924 1.0739505548279389 8 0.1621394006072901 0.87017579309806 9 0.1380626698045056 0.8335798814935966 >10 0.8330692589648313 10.447425982694869 >50 0.01677800286489908 0.6969309397494615 >100 0.001097636465165585 0.15827905170650922 >500 8.37967177087798E-5 0.03510652943208408 >1k 8.3796717583028E-5 0.10922655979024021 >5k 8.3796717583028E-5 0.4915476267657542 >10k+ 1.67593435166056E-4 9.709358583268614 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 288303 2.8828452096220634 No Hit AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 27309 0.273072496053003 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 19404 0.1940275628332224 No Hit GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT 10418 0.10417332249002839 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 9.999359041085466E-6 0.0 0.0 0.0 0.0 4 9.999359041085466E-6 0.0 0.0 0.0 0.0 5 9.999359041085466E-6 0.0 0.0 0.0 0.0 6 2.99980771232564E-5 0.0 0.0 0.0 9.999359041085466E-6 7 3.9997436164341864E-5 9.999359041085466E-6 0.0 0.0 9.999359041085466E-6 8 5.99961542465128E-5 9.999359041085466E-6 0.0 9.999359041085466E-6 9.999359041085466E-6 9 5.99961542465128E-5 2.99980771232564E-5 0.0 9.999359041085466E-6 9.999359041085466E-6 10-11 5.99961542465128E-5 2.99980771232564E-5 0.0 9.999359041085466E-6 1.4999038561628198E-5 12-13 1.0999294945194014E-4 2.99980771232564E-5 0.0 9.999359041085466E-6 2.9998077123256396E-5 14-15 1.349913470546538E-4 2.99980771232564E-5 0.0 9.999359041085466E-6 4.999679520542733E-5 16-17 1.6498942417791018E-4 2.99980771232564E-5 0.0 9.999359041085466E-6 4.999679520542733E-5 18-19 1.9498750130116657E-4 2.99980771232564E-5 0.0 9.999359041085466E-6 5.99961542465128E-5 20-21 2.399846169860512E-4 2.99980771232564E-5 0.0 9.999359041085466E-6 6.499583376705553E-5 22-23 2.5998333506822217E-4 2.99980771232564E-5 0.0 9.999359041085466E-6 7.499519280814099E-5 24-25 2.9998077123256396E-4 2.99980771232564E-5 0.0 1.4999038561628198E-5 9.999359041085466E-5 26-27 3.2497916883527766E-4 2.99980771232564E-5 0.0 2.99980771232564E-5 1.0999294945194014E-4 28-29 3.4997756643799136E-4 2.99980771232564E-5 0.0 2.99980771232564E-5 1.0999294945194014E-4 30-31 3.6997628452016226E-4 2.99980771232564E-5 0.0 3.499775664379913E-5 1.0999294945194014E-4 32-33 4.199730797255896E-4 2.99980771232564E-5 0.0 3.9997436164341864E-5 1.1499262897248286E-4 34-35 5.299660291775297E-4 2.99980771232564E-5 0.0 3.9997436164341864E-5 1.199923084930256E-4 36-37 5.949618629445852E-4 3.9997436164341864E-5 0.0 4.999679520542733E-5 1.3499134705465378E-4 38-39 6.549580171910981E-4 3.9997436164341864E-5 0.0 4.999679520542733E-5 1.3999102657519653E-4 40-41 7.599512871224954E-4 3.9997436164341864E-5 0.0 4.999679520542733E-5 1.49990385616282E-4 42-43 8.24947120889551E-4 3.9997436164341864E-5 0.0 4.999679520542733E-5 1.8498814226008113E-4 44-45 9.149413522593202E-4 4.999679520542733E-5 0.0 4.999679520542733E-5 1.8998782178062385E-4 46-47 9.799371860263757E-4 4.999679520542733E-5 0.0 5.99961542465128E-5 1.8998782178062385E-4 48-49 0.0010499326993139739 4.999679520542733E-5 0.0 6.999551328759827E-5 1.9498750130116657E-4 50-51 0.0011149285330810295 4.999679520542733E-5 0.0 6.999551328759827E-5 1.9998718082170933E-4 52-53 0.0011849240463686276 4.999679520542733E-5 0.0 6.999551328759827E-5 2.099865398627948E-4 54-55 0.0013199153934232814 4.999679520542733E-5 0.0 6.999551328759827E-5 2.2998525794496572E-4 56-57 0.00144490738143685 4.999679520542733E-5 0.0 6.999551328759827E-5 2.4998397602713667E-4 58-59 0.0015698993694504182 4.999679520542733E-5 0.0 6.999551328759827E-5 2.5498365554767937E-4 60-61 0.0016798923189023582 4.999679520542733E-5 0.0 7.999487232868373E-5 2.599833350682221E-4 62-63 0.001819883345477555 4.999679520542733E-5 0.0 7.999487232868373E-5 2.6998269410930757E-4 64-65 0.0020148708467787216 4.999679520542733E-5 0.0 7.999487232868373E-5 2.8498173267093576E-4 66-67 0.0021848599504771743 4.999679520542733E-5 0.0 7.999487232868373E-5 2.899814121914785E-4 68-69 0.0022948528999291147 4.999679520542733E-5 0.0 8.499455184922646E-5 2.99980771232564E-4 70-71 0.002454842644586482 4.999679520542733E-5 0.0 9.999359041085466E-5 3.0498045075310677E-4 72-73 0.002624831748284935 5.499647472597007E-5 0.0 1.199923084930256E-4 3.1497980979419216E-4 74-75 0.002799820531503931 5.99961542465128E-5 0.0 1.199923084930256E-4 3.199794893147349E-4 76-77 0.0030298057894488964 5.99961542465128E-5 0.0 1.3499134705465378E-4 3.199794893147349E-4 78-79 0.003334786240202003 5.99961542465128E-5 0.0 1.3999102657519653E-4 3.199794893147349E-4 80-81 0.003689763486160537 6.999551328759827E-5 0.0 1.3999102657519653E-4 3.2497916883527766E-4 82-83 0.0040947375273244985 7.999487232868373E-5 0.0 1.3999102657519653E-4 3.3497852787636316E-4 84-85 0.0046447022745842 7.999487232868373E-5 0.0 1.3999102657519653E-4 3.5497724595853406E-4 86-87 0.005474649074994293 7.999487232868373E-5 0.0 1.3999102657519653E-4 3.6997628452016226E-4 88-89 0.006664572800883463 7.999487232868373E-5 0.0 1.3999102657519653E-4 3.799756435612477E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTCACG 750 0.0 30.39909 3 AAGCAGT 12170 0.0 30.332487 1 CGCGTAG 465 0.0 24.516008 54-55 AGTGGTA 15010 0.0 23.732874 5 AGCAGTG 16570 0.0 22.4449 2 GGTATCA 16140 0.0 22.130136 8 GTATCAA 16140 0.0 22.100708 9 CTCGCGT 535 0.0 21.753153 52-53 TGGTATC 16830 0.0 21.22284 7 GTGTACG 1100 0.0 20.294338 9 GTGGTAT 17635 0.0 20.280994 6 ATCAACG 14665 0.0 19.87005 10-11 GCAGTGG 18625 0.0 19.86652 3 CAACGCA 15105 0.0 19.35409 12-13 ACGTACG 640 0.0 19.295815 20-21 ACTCGCG 595 0.0 19.1611 50-51 CGCTAGT 625 0.0 18.239544 90-91 ACGTGTA 1335 0.0 18.145071 7 CAGTGGT 20605 0.0 17.634842 4 CGTACGC 690 0.0 17.2092 20-21 >>END_MODULE