FastQCFastQC Report
Sat 18 Jun 2016
SRR578609_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR578609_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9897814
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT151178515.273928162319478No Hit
AAGCAGTGGTATCAACGCAGAGTATTTTTTTTTTTTTTTTTTTTTTTTTT317500.3207779010597694No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA234890.23731502733835974No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT3126700.092.111841
AGTGGTA3136900.091.259025
AGCAGTG3158100.091.004212
GGTATCA3162350.090.757738
GTATCAA3170000.090.696679
TGGTATC3155050.090.658727
GTGGTAT3165650.090.501666
GCAGTGG3173150.090.421413
CAGTGGT3181200.089.938434
GTATCGA6900.046.1385089
ATCAACG3168100.045.86274710-11
CAACGCA3171600.045.79379312-13
TCAACGC3169100.045.71597712-13
ACGCAGA3184150.045.65037514-15
AACGCAG3185850.045.5283314-15
TATCAAC3181300.045.4760410-11
CGCAGAG3191100.045.38435416-17
AGAGTAC3113500.045.34187718-19
GAGTACT1807750.045.23084320-21
CAGAGTA3160450.045.23044218-19
Produced by FastQC (version 0.11.5)