##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR578609_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 9897814 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.66857954695855 31.0 31.0 34.0 27.0 34.0 2 30.963928701832547 31.0 31.0 34.0 27.0 34.0 3 31.082175821853188 31.0 31.0 34.0 27.0 34.0 4 34.52424020091709 37.0 35.0 37.0 30.0 37.0 5 34.56078564418365 37.0 35.0 37.0 30.0 37.0 6 34.569838653262224 37.0 35.0 37.0 30.0 37.0 7 34.44463504769841 37.0 35.0 37.0 30.0 37.0 8 34.42771686758309 37.0 35.0 37.0 30.0 37.0 9 35.86945056756977 39.0 35.0 39.0 30.0 39.0 10-11 35.81752389972169 39.0 35.0 39.0 30.0 39.0 12-13 35.90929476953194 39.0 35.0 39.0 30.0 39.0 14-15 36.96506662986393 40.0 36.5 41.0 31.0 41.0 16-17 36.92836271726262 40.0 36.5 41.0 30.5 41.0 18-19 36.88482103219964 40.0 36.0 41.0 30.5 41.0 20-21 36.74011382715416 39.5 36.0 41.0 30.0 41.0 22-23 36.40786839397063 39.0 36.0 40.5 29.5 41.0 24-25 36.325173113982544 39.0 36.0 40.0 30.0 41.0 26-27 36.278200014669906 39.0 36.0 40.5 29.0 41.0 28-29 35.989451812289055 39.0 35.0 41.0 27.0 41.0 30-31 35.70154566452754 39.0 34.5 41.0 26.5 41.0 32-33 35.33007242811392 38.0 34.0 40.0 25.0 41.0 34-35 35.11937054990122 38.0 34.0 40.0 25.0 41.0 36-37 35.088969695732814 38.0 34.0 40.0 25.0 41.0 38-39 34.26803317378969 36.5 33.5 40.0 25.0 41.0 40-41 34.23286510536569 37.0 33.0 40.0 24.5 41.0 42-43 34.05014455717192 37.0 33.0 40.0 23.5 41.0 44-45 33.898563662643085 36.0 33.0 40.0 23.0 41.0 46-47 33.71617081307044 36.0 33.0 40.0 23.0 41.0 48-49 33.39145037479993 36.0 33.0 40.0 21.5 41.0 50-51 32.12193818756344 35.5 31.5 39.0 17.5 40.5 52-53 31.707921870425125 35.0 31.0 39.0 10.5 40.0 54-55 31.205797866074267 35.0 30.5 39.5 2.0 41.0 56-57 30.593308482054724 35.0 28.5 39.5 2.0 41.0 58-59 30.310567919340574 35.0 28.0 39.0 2.0 41.0 60-61 30.048909991640578 35.0 28.0 39.0 2.0 41.0 62-63 29.71964375164051 35.0 28.0 39.0 2.0 40.5 64-65 29.386625218457326 35.0 28.0 38.0 2.0 40.0 66-67 29.082310548571634 35.0 27.5 38.0 2.0 40.0 68-69 28.750279758742685 35.0 26.5 37.0 2.0 40.0 70-71 28.367067162506792 34.5 26.0 36.5 2.0 39.0 72-73 27.988940133649713 34.0 26.0 36.0 2.0 39.0 74-75 27.600355442120858 34.0 25.5 35.5 2.0 39.0 76-77 27.21133863497536 34.0 25.0 35.0 2.0 37.5 78-79 26.841067229592312 34.0 24.0 35.0 2.0 37.0 80-81 26.471430762388543 33.5 23.0 35.0 2.0 36.5 82-83 26.12864113227426 33.0 21.5 35.0 2.0 36.0 84-85 25.743925224297 33.0 20.0 35.0 2.0 36.0 86-87 25.400095263459185 33.0 18.5 35.0 2.0 35.0 88-89 25.020615612699935 32.5 17.0 35.0 2.0 35.0 90-91 24.64044565800085 32.0 9.0 35.0 2.0 35.0 92-93 24.26287718682125 32.0 4.5 35.0 2.0 35.0 94-95 23.826689559937172 32.0 2.0 35.0 2.0 35.0 96-97 23.346025496134803 31.5 2.0 35.0 2.0 35.0 98-99 22.739421704630942 31.0 2.0 34.5 2.0 35.0 100-101 21.206323840799595 29.0 2.0 33.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.42226030685857197 1101 2 0.47748881567769175 1101 3 0.5207058677770355 1101 4 0.6055655346624889 1101 5 0.5693515585130129 1101 6 0.45892726615792157 1101 7 0.5619192889138773 1101 8 0.6278162348634098 1101 9 0.5509261659290203 1101 10-11 0.6485421596525427 1101 12-13 0.5882012359471105 1101 14-15 0.7802029725274195 1101 16-17 0.6673229121205466 1101 18-19 0.6505447307194174 1101 20-21 0.6449226261497287 1101 22-23 0.742938535911911 1101 24-25 0.8837481472691664 1101 26-27 0.8274611922619357 1101 28-29 0.8313804056658327 1101 30-31 0.9091297630145263 1101 32-33 1.0009006099729305 1101 34-35 0.9622167915928017 1101 36-37 0.9417330065369072 1101 38-39 1.0028747425056324 1101 40-41 0.9524414946575916 1101 42-43 1.154142990781736 1101 44-45 1.0613764121936313 1101 46-47 1.0337837894883464 1101 48-49 1.0743109433487277 1101 50-51 1.1459093764619936 1101 52-53 1.130961506839423 1101 54-55 0.975963969323999 1101 56-57 1.0034602624723377 1101 58-59 1.0837820799636795 1101 60-61 1.2093136570992407 1101 62-63 1.1496856389476413 1101 64-65 1.1321424259007422 1101 66-67 0.9969005825989008 1101 68-69 1.1591800572591815 1101 70-71 1.0869930588496075 1101 72-73 1.1968956388804948 1101 74-75 1.192949229235083 1101 76-77 1.0678890429888241 1101 78-79 1.0983866019379214 1101 80-81 1.2103922064139176 1101 82-83 1.2228148239150904 1101 84-85 1.3453815538116771 1101 86-87 1.3078283040572778 1101 88-89 1.4385417695847806 1101 90-91 1.5593800302670928 1101 92-93 1.51955359632651 1101 94-95 1.6135276587788248 1101 96-97 1.799500690883061 1101 98-99 1.9596294094617583 1101 100-101 2.2816851100256983 1102 1 0.5586578671019282 1102 2 0.5503970854752929 1102 3 0.5238136751181521 1102 4 0.5864108622802959 1102 5 0.5573319718704681 1102 6 0.45658519334683234 1102 7 0.6144118601676851 1102 8 0.6809839902675776 1102 9 0.6177272727160243 1102 10-11 0.7188996934963257 1102 12-13 0.7054096212911674 1102 14-15 0.836357706384895 1102 16-17 0.8449448086097178 1102 18-19 0.728412977790498 1102 20-21 0.6931409449709633 1102 22-23 0.782742991361765 1102 24-25 0.8805239766973045 1102 26-27 1.018032556651626 1102 28-29 0.8428165861100823 1102 30-31 0.9201499365719954 1102 32-33 0.9803616273965332 1102 34-35 1.0574560966824365 1102 36-37 0.9148359915483439 1102 38-39 0.9744159702829478 1102 40-41 0.9735704224301287 1102 42-43 1.1689908655639414 1102 44-45 1.057759940414556 1102 46-47 1.0467358623554972 1102 48-49 1.1542027855211927 1102 50-51 1.0916858073039535 1102 52-53 1.1560941039901813 1102 54-55 1.1414403176769525 1102 56-57 0.968926036304012 1102 58-59 1.0088777339514259 1102 60-61 1.0054084013301825 1102 62-63 1.0109991733102923 1102 64-65 1.0638566621254917 1102 66-67 1.023249964975328 1102 68-69 1.1683723030226538 1102 70-71 1.1085102281411174 1102 72-73 1.1579288227721953 1102 74-75 1.2771101960672375 1102 76-77 1.1213420301557626 1102 78-79 1.1203101315236736 1102 80-81 1.171897034253174 1102 82-83 1.3764016503040715 1102 84-85 1.4685426503605363 1102 86-87 1.464692656920743 1102 88-89 1.6515316168844443 1102 90-91 1.610070447914289 1102 92-93 1.5773620200249603 1102 94-95 1.6012018943153628 1102 96-97 1.8174631971489994 1102 98-99 1.9205238776725615 1102 100-101 2.2406710607979186 1103 1 0.3768617292637586 1103 2 0.3049494293246475 1103 3 0.169229434887896 1103 4 0.36063006261240105 1103 5 0.3272239837381079 1103 6 0.2198382116537303 1103 7 0.49599455540277404 1103 8 0.3493676278943596 1103 9 0.5409295313377385 1103 10-11 0.510040024376913 1103 12-13 0.39325070534647466 1103 14-15 0.6411731745219171 1103 16-17 0.5939885617838954 1103 18-19 0.5609542006018842 1103 20-21 0.7412426173772317 1103 22-23 0.7281539867707565 1103 24-25 0.6033908474522747 1103 26-27 0.7637996505826763 1103 28-29 0.6273767243246979 1103 30-31 0.5414472528547947 1103 32-33 0.8172388545408538 1103 34-35 0.7490675665330357 1103 36-37 0.7384553658772575 1103 38-39 0.5635584869680699 1103 40-41 0.6450984623141025 1103 42-43 0.4420268436350625 1103 44-45 0.590153033884981 1103 46-47 0.7475458093700453 1103 48-49 0.6535971258777238 1103 50-51 0.6260192070691133 1103 52-53 0.5756635138441872 1103 54-55 0.6232145713065691 1103 56-57 0.471463631906893 1103 58-59 0.6350014822782128 1103 60-61 0.5404375627063835 1103 62-63 0.49803932528751815 1103 64-65 0.5808611263296157 1103 66-67 0.5886101028714386 1103 68-69 0.6554958644909483 1103 70-71 0.7417517285632584 1103 72-73 0.7383600575193192 1103 74-75 0.8653623384749451 1103 76-77 0.8356578402726491 1103 78-79 0.843511398597169 1103 80-81 0.7755206292028056 1103 82-83 0.8753468458605091 1103 84-85 0.8519943950265976 1103 86-87 1.054732856522623 1103 88-89 1.1233134532984757 1103 90-91 0.876526375510867 1103 92-93 1.0223877430228647 1103 94-95 1.0549613180995827 1103 96-97 1.2148768033442643 1103 98-99 1.3193507055016447 1103 100-101 1.6922334090085691 1104 1 0.1500106917346642 1104 2 0.2567305402604241 1104 3 0.22142497375421044 1104 4 0.14520936188385036 1104 5 0.085088694332633 1104 6 0.14834901206767626 1104 7 0.3467181374788524 1104 8 0.22988714012861067 1104 9 0.33878757770093415 1104 10-11 0.39795256354398134 1104 12-13 0.22547736939688434 1104 14-15 0.3834549489585726 1104 16-17 0.4601089491014889 1104 18-19 0.5915855421520035 1104 20-21 0.4067436577932213 1104 22-23 0.33672392693301845 1104 24-25 0.37677631717206594 1104 26-27 0.47912956595676803 1104 28-29 0.6763952318056567 1104 30-31 0.4958100182137315 1104 32-33 0.7206253781338958 1104 34-35 0.6417593520135867 1104 36-37 0.6486497208020339 1104 38-39 0.45030522306007725 1104 40-41 0.5129093557600015 1104 42-43 0.6272280453855998 1104 44-45 0.8321647602816213 1104 46-47 0.654214444557276 1104 48-49 0.6044723469500681 1104 50-51 0.568809245476487 1104 52-53 0.4651325634928618 1104 54-55 0.6426934415946519 1104 56-57 0.654952261708992 1104 58-59 0.7696938356069545 1104 60-61 0.6958371386927062 1104 62-63 0.6734328237271647 1104 64-65 0.8579027098004168 1104 66-67 0.6257343143231573 1104 68-69 0.6661239118961078 1104 70-71 0.7883209300091387 1104 72-73 0.5755064874185258 1104 74-75 0.6664845720250945 1104 76-77 0.7139915147839524 1104 78-79 0.8224731979440918 1104 80-81 0.7209735588717869 1104 82-83 0.7542907040484046 1104 84-85 0.7127108899819063 1104 86-87 0.7793584383632108 1104 88-89 0.9246043772981345 1104 90-91 0.9929933665143764 1104 92-93 0.8879605238779469 1104 94-95 0.9311420163012194 1104 96-97 1.068201574283183 1104 98-99 1.257821334024431 1104 100-101 1.451282247266871 1105 1 0.002895281967877139 1105 2 0.1570100461302779 1105 3 0.13004745255164707 1105 4 0.14628434108959 1105 5 0.1512785126473517 1105 6 0.20101206801885496 1105 7 0.2962820611829926 1105 8 0.28329869690143994 1105 9 0.1861584525053246 1105 10-11 0.26302914621130924 1105 12-13 0.16493161939732204 1105 14-15 0.04939370186512093 1105 16-17 0.2622713993179744 1105 18-19 0.16263870252321055 1105 20-21 0.18320817288704205 1105 22-23 0.3341063553861261 1105 24-25 0.47672027735431755 1105 26-27 0.46735813095754253 1105 28-29 0.5836504841636909 1105 30-31 0.4531413952524517 1105 32-33 0.5979077529133718 1105 34-35 0.45050406379302643 1105 36-37 0.6783726282583231 1105 38-39 0.5357155850931932 1105 40-41 0.45922953712825887 1105 42-43 0.6720477266657241 1105 44-45 0.7455067705591887 1105 46-47 0.5056240682660373 1105 48-49 0.6056636991271844 1105 50-51 0.6056588963668119 1105 52-53 0.45773657382903465 1105 54-55 0.6259798316829901 1105 56-57 0.6606881075881219 1105 58-59 0.6852070029306887 1105 60-61 0.5688246109327579 1105 62-63 0.6592279962060417 1105 64-65 0.7725485308502975 1105 66-67 0.6988851505346574 1105 68-69 0.7078900654914229 1105 70-71 0.6436365789272109 1105 72-73 0.5622599102793906 1105 74-75 0.5578774226087795 1105 76-77 0.5386323557490904 1105 78-79 0.6493260034426704 1105 80-81 0.7014448839634575 1105 82-83 0.8055850177214623 1105 84-85 0.8801707177142326 1105 86-87 0.888611787973943 1105 88-89 0.8585082313457839 1105 90-91 0.9611381222716311 1105 92-93 1.174775287370597 1105 94-95 1.2628462913268628 1105 96-97 1.3635370936284765 1105 98-99 1.5734395147721152 1105 100-101 1.8604250746739375 1106 1 0.32843072390861394 1106 2 0.3294058243890845 1106 3 0.28045908218108906 1106 4 0.08825698413694028 1106 5 0.3226408987986318 1106 6 0.24927514030743936 1106 7 0.344985584400483 1106 8 0.37942650428294655 1106 9 0.42337942321769617 1106 10-11 0.29732552917836585 1106 12-13 0.41003742327072956 1106 14-15 0.3057538963131279 1106 16-17 0.4343602014919199 1106 18-19 0.43207436244151154 1106 20-21 0.43014883725182784 1106 22-23 0.5025261935235008 1106 24-25 0.6747618281948391 1106 26-27 0.4521714737626539 1106 28-29 0.5495119359451834 1106 30-31 0.6395862782159583 1106 32-33 0.5513370746689503 1106 34-35 0.5105954065536267 1106 36-37 0.6682715484172448 1106 38-39 0.6265826164855852 1106 40-41 0.47499461589767833 1106 42-43 0.7296524782692515 1106 44-45 0.6681534076651943 1106 46-47 0.6657366743331181 1106 48-49 0.7910499125681199 1106 50-51 0.656842106474663 1106 52-53 0.6304065501090896 1106 54-55 0.7914943268583698 1106 56-57 0.567244867197843 1106 58-59 0.6517573674920278 1106 60-61 0.6748485951167105 1106 62-63 0.7533794629727772 1106 64-65 0.8735235463504907 1106 66-67 0.8543447625240397 1106 68-69 0.8142677729216281 1106 70-71 0.7953982335473491 1106 72-73 0.745475041496011 1106 74-75 0.6960445760530121 1106 76-77 0.8024537687474158 1106 78-79 0.9636177208686938 1106 80-81 1.0492612203760316 1106 82-83 1.1340639251866271 1106 84-85 1.214203912786374 1106 86-87 1.24917986920482 1106 88-89 1.2583778989753043 1106 90-91 1.1339721494340722 1106 92-93 1.3559135417563546 1106 94-95 1.3247185678975661 1106 96-97 1.4922315701921605 1106 98-99 1.6669252358998357 1106 100-101 1.822867515224413 1107 1 0.11539459143851971 1107 2 0.14637748887874835 1107 3 0.17360098250305356 1107 4 0.06359773232029653 1107 5 0.19415674533598093 1107 6 0.21490429366373576 1107 7 0.2500675142315103 1107 8 0.28374895671382916 1107 9 0.2691410585619636 1107 10-11 0.21736355565326448 1107 12-13 0.233920021046643 1107 14-15 0.24169502183365665 1107 16-17 0.13521004494662492 1107 18-19 0.16455186756077467 1107 20-21 0.20803623396612636 1107 22-23 0.3627686746263379 1107 24-25 0.36675508927954326 1107 26-27 0.28194241148143107 1107 28-29 0.39728159147691855 1107 30-31 0.45232716001573436 1107 32-33 0.3733995889494537 1107 34-35 0.4416442543121022 1107 36-37 0.3115631096549407 1107 38-39 0.4086999038937833 1107 40-41 0.31283983263784165 1107 42-43 0.37759641609300587 1107 44-45 0.42412178709376036 1107 46-47 0.5057911821491743 1107 48-49 0.4467223551756092 1107 50-51 0.45043263503260533 1107 52-53 0.5131942982926851 1107 54-55 0.6902291563510552 1107 56-57 0.5044749497852337 1107 58-59 0.5472053290181798 1107 60-61 0.5057526879373562 1107 62-63 0.589249022969458 1107 64-65 0.5620584017796695 1107 66-67 0.6607945110154851 1107 68-69 0.582378802177498 1107 70-71 0.6345519169075295 1107 72-73 0.6129257192642932 1107 74-75 0.6595070025519725 1107 76-77 0.6785914810338802 1107 78-79 0.7438878218266289 1107 80-81 0.7728802905182484 1107 82-83 0.8293938459110599 1107 84-85 0.9719922613518754 1107 86-87 1.0037515430261301 1107 88-89 1.0734595383358396 1107 90-91 1.0644696750600886 1107 92-93 1.04201343112598 1107 94-95 1.0795141098388719 1107 96-97 1.118060251613965 1107 98-99 1.3127438217568503 1107 100-101 1.5651560155435895 1108 1 0.15029686854877156 1108 2 0.12897010969884093 1108 3 -0.09263683620878282 1108 4 0.19595325025899513 1108 5 0.24641963379388443 1108 6 0.22054956302913098 1108 7 0.2268375034685235 1108 8 0.13884164545211775 1108 9 0.129710752751393 1108 10-11 0.15253019864732664 1108 12-13 0.1938263778069782 1108 14-15 0.15859987473013604 1108 16-17 0.24573744296494482 1108 18-19 0.08694420080882992 1108 20-21 0.12592682604688576 1108 22-23 0.2987700915184419 1108 24-25 0.35418814427286094 1108 26-27 0.36649759925564496 1108 28-29 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2314 5 -0.8357714919277228 2314 6 -0.7011863972915364 2314 7 -0.5627644081854655 2314 8 -0.5838608648836399 2314 9 -0.9284906021445067 2314 10-11 -2.276904876320316 2314 12-13 -1.9866802102644456 2314 14-15 -2.9481429855705343 2314 16-17 -2.310870901247256 2314 18-19 -1.5870123021861104 2314 20-21 -1.1927073523350273 2314 22-23 -1.201603366615963 2314 24-25 -1.0154251106518544 2314 26-27 -0.8732595580278755 2314 28-29 -1.5373971463437996 2314 30-31 -1.2129188866604963 2314 32-33 -1.4274139348346608 2314 34-35 -1.3281756403148322 2314 36-37 -0.9691338130016476 2314 38-39 -1.5347428053788406 2314 40-41 -2.79264808909641 2314 42-43 -1.6090236097261723 2314 44-45 -3.0993444983264027 2314 46-47 -2.4559838006402686 2314 48-49 -1.6146104831636912 2314 50-51 -1.485071996467191 2314 52-53 -1.5651991675481973 2314 54-55 -1.0254959883497072 2314 56-57 -0.9621875440242462 2314 58-59 -1.1820700047719832 2314 60-61 -0.9052022069951065 2314 62-63 -1.5494776836783366 2314 64-65 -1.23305080640284 2314 66-67 -0.8407818279128989 2314 68-69 -1.3038904459316214 2314 70-71 -2.165298093160356 2314 72-73 -1.23042689197543 2314 74-75 -1.3059624604840714 2314 76-77 -1.0386280768206753 2314 78-79 -1.4323580895043193 2314 80-81 -2.304338922406547 2314 82-83 -1.2872380923746647 2314 84-85 -1.545479883215723 2314 86-87 -1.0229501786144581 2314 88-89 -0.9850968334708909 2314 90-91 -1.5617457749089638 2314 92-93 -1.0715380415382612 2314 94-95 -0.9956876207966552 2314 96-97 -1.1164854999243055 2314 98-99 -1.1366955162238135 2314 100-101 -1.1882669142868423 2315 1 -0.7541987348255041 2315 2 -0.5866074565968198 2315 3 -0.6869603590047433 2315 4 -0.5974996989109158 2315 5 -0.5467359643452312 2315 6 -0.6291850811022144 2315 7 -1.2275107658024282 2315 8 -0.7567990446399975 2315 9 -3.12405823085421 2315 10-11 -1.1640863170806455 2315 12-13 -0.8897418791826013 2315 14-15 -1.1076980523485602 2315 16-17 -1.4934923632151609 2315 18-19 -0.7599662178365989 2315 20-21 -1.063142469070037 2315 22-23 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2315 88-89 -1.8363013015108862 2315 90-91 -1.8239664267351543 2315 92-93 -1.795778003202198 2315 94-95 -1.9212008486654533 2315 96-97 -1.9033527762080702 2315 98-99 -1.960294369635637 2315 100-101 -2.0182395641002238 2316 1 -1.246837332257364 2316 2 -1.1209185637207817 2316 3 -2.2748143187263246 2316 4 -1.4035305369513793 2316 5 -1.213335700354989 2316 6 -2.6694391256453507 2316 7 -6.958783395815914 2316 8 -7.720483129254607 2316 9 -4.303096075273679 2316 10-11 -2.1425693133024026 2316 12-13 -2.1371042509028797 2316 14-15 -2.17083061371212 2316 16-17 -2.1554969187158832 2316 18-19 -1.6818363256888205 2316 20-21 -2.735991420072132 2316 22-23 -3.2723175401724305 2316 24-25 -2.0529079949096527 2316 26-27 -2.255231588833766 2316 28-29 -3.080948925200765 2316 30-31 -3.583882952142744 2316 32-33 -3.5502983644531234 2316 34-35 -2.782188201291376 2316 36-37 -3.8662426101426703 2316 38-39 -3.389352554818352 2316 40-41 -4.1000905100716665 2316 42-43 -3.4780560246303978 2316 44-45 -3.0832928927468686 2316 46-47 -2.8142011116265415 2316 48-49 -2.8320393753268753 2316 50-51 -2.479937805461617 2316 52-53 -2.862320485312832 2316 54-55 -4.417474160016809 2316 56-57 -3.0900928558612506 2316 58-59 -2.523666939108825 2316 60-61 -3.048986598174597 2316 62-63 -2.7961719835327123 2316 64-65 -4.392205157093656 2316 66-67 -3.226613128813838 2316 68-69 -3.1704793818922283 2316 70-71 -3.5120556690343143 2316 72-73 -3.110179717894674 2316 74-75 -2.839503818783829 2316 76-77 -3.3978934001875807 2316 78-79 -4.464653432034417 2316 80-81 -3.4390041913001603 2316 82-83 -3.183269130867288 2316 84-85 -3.210382474001907 2316 86-87 -4.395172109704497 2316 88-89 -4.8561917021217305 2316 90-91 -4.202322028728439 2316 92-93 -4.2052463989719975 2316 94-95 -4.294670934309547 2316 96-97 -4.472680519651057 2316 98-99 -4.703344798543132 2316 100-101 -4.757785052998063 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 97912.0 3 17718.0 4 15584.0 5 15152.0 6 17155.0 7 21330.0 8 26794.0 9 31296.0 10 36598.0 11 44021.0 12 44495.0 13 50457.0 14 56942.0 15 67144.0 16 83585.0 17 108657.0 18 151856.0 19 226922.0 20 324298.0 21 247401.0 22 143222.0 23 132885.0 24 144039.0 25 152296.0 26 166367.0 27 185664.0 28 213710.0 29 248449.0 30 294625.0 31 349447.0 32 426346.0 33 528276.0 34 683389.0 35 966523.0 36 1374273.0 37 1270235.0 38 822775.0 39 109913.0 40 63.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 10.866883039702277 50.00424350109577 21.560900310094222 17.567973149107726 2 16.342820765732124 51.3945518233008 16.653979850517413 15.608647560449661 3 47.80885725483255 18.785987180621753 17.27084286779858 16.134312696747113 4 16.256335578342735 17.387436037457274 18.275246243148676 48.08098214105131 5 16.512808605237115 49.11147904990616 19.137878411240642 15.237833933616082 6 48.116463930783056 18.353417825353656 17.252513085315112 16.27760515854818 7 17.274943134309822 17.88003736165851 49.59019891563813 15.254820588393533 8 47.56045936802412 18.615272099769612 18.044783548474587 15.779484983731676 9 47.10311778244286 18.3253851723315 18.68942308081237 15.882073964413271 10-11 15.883420748442663 33.98326244674406 33.82684144026234 16.306475364550934 12-13 15.741756836301041 18.747342227657576 33.65491999536539 31.855980940675995 14-15 16.07121777054983 49.76501777779958 17.83104020135079 16.33272425029981 16-17 31.76431336588167 18.425067208038072 17.984254261227207 31.826365164853055 18-19 16.210391359073274 34.08683598993719 17.99339151056437 31.70938114042517 20-21 31.843497652677083 34.03320016336235 18.135829326777273 15.987472857183294 22-23 31.71129323460428 18.410926978300076 33.75975798153789 16.11802180555776 24-25 16.031264831996737 33.72167147060579 18.704138790114904 31.542924907282565 26-27 16.317202591994025 24.475222691682838 42.96252638252198 16.245048333801154 28-29 28.99590640854252 18.19032498912252 36.70438419162195 16.109384410713012 30-31 26.22163232400128 19.854506558555197 37.09660783311235 16.827253284331174 32-33 19.908874971108983 22.116787882154277 38.97186549606903 19.00247165066771 34-35 19.283040252078877 21.206234502037518 39.71448341001197 19.796241835871633 36-37 19.312160861724582 21.10668248975004 39.8236885305558 19.75746811796958 38-39 19.65286956275616 21.12854288612575 39.5136736943564 19.70491385676169 40-41 19.537461269232452 20.991857409415758 39.40850385451469 20.062177466837092 42-43 20.05351974692368 21.219401547601816 39.33648836387484 19.390590341599662 44-45 19.479045526658005 21.32190504493331 39.260663732267126 19.938385696141562 46-47 19.773892914205142 21.190114860373914 39.22692296476154 19.809069260659406 48-49 19.677711268860257 21.28252902243684 39.35852574435895 19.681233964343953 50-51 19.844073646736987 21.038033886103186 38.96364611077942 20.154246356380405 52-53 20.192009410896137 21.894792925210442 37.87684546971747 20.036352194175947 54-55 20.801705700031885 22.582139021709043 36.419527934466785 20.196627343792287 56-57 21.124699880331182 23.42534021311946 35.14451988282382 20.305440023725538 58-59 21.166158499218707 24.32372588807499 34.17277369766329 20.337341915043012 60-61 21.109297880491198 24.650304801315635 33.723857386744406 20.51653993144876 62-63 20.872496488350006 24.829663936447563 33.544504916613256 20.753334658589175 64-65 20.681428046662425 24.47735443465083 33.822025056096116 21.019192462590627 66-67 20.680312801227448 23.96452555550934 34.762185060094 20.59297658316921 68-69 20.539079612999014 23.586539798362907 35.2883512735271 20.586029315110988 70-71 20.388027273212476 23.507164413980938 35.76312289846822 20.34168541433837 72-73 20.098686336818343 23.295459972645304 35.730864284757295 20.874989405779054 74-75 20.583137149838297 23.47476082342333 35.213136872373916 20.728965154364456 76-77 20.86990812693847 24.048918914370525 34.358208362348314 20.722964596342695 78-79 20.773308552429185 24.222004595828718 34.15518884547985 20.849498006262245 80-81 20.691304208947727 24.882001519469878 33.54592130698669 20.880772964595707 82-83 21.520281319903958 24.93298417656398 32.86651858937377 20.680215914158296 84-85 21.380372893364157 25.3859231165512 32.32095266364569 20.912751326438954 86-87 21.154118693582134 25.698186541591273 32.19312725545099 20.9545675093756 88-89 21.000641808331743 25.30620079640029 32.16914790606683 21.52400948920113 90-91 21.011794679128144 25.25927805942652 32.27326669785877 21.455660563586562 92-93 21.11405145989968 25.568009418459837 32.28447884636804 21.03346027527244 94-95 21.141450173984573 25.37340527620114 31.757217888359175 21.727926661455115 96-97 21.045833139416104 25.154437702946698 32.128974690504315 21.670754467132884 98-99 21.415262876267093 25.501527330625073 31.54309031016619 21.540119482941645 100-101 21.823607392208412 25.12787770307634 31.26728077068599 21.781234134029255 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 27192.0 1 15790.0 2 3202.5 3 1816.5 4 1472.5 5 1263.0 6 1479.0 7 1915.5 8 1737.5 9 1740.5 10 2813.5 11 17610.5 12 325703.5 13 354962.5 14 77416.0 15 64923.0 16 66789.5 17 69662.5 18 72409.5 19 72973.0 20 72089.5 21 71442.0 22 70441.5 23 70066.5 24 71503.0 25 73939.5 26 77088.5 27 81813.0 28 85583.5 29 89283.5 30 95083.0 31 102590.5 32 109838.5 33 118307.0 34 133640.5 35 145547.5 36 153751.5 37 164965.5 38 175724.0 39 186669.0 40 199810.5 41 215360.5 42 229832.5 43 243180.5 44 256094.5 45 268546.5 46 277085.0 47 287008.5 48 299954.0 49 310191.5 50 317627.0 51 321653.5 52 327638.5 53 335577.0 54 326318.0 55 306524.5 56 293074.5 57 276489.5 58 275259.5 59 246198.5 60 189749.5 61 155627.5 62 125099.5 63 98166.0 64 77667.0 65 62889.0 66 50817.0 67 41321.5 68 32443.0 69 24776.0 70 19719.0 71 16127.5 72 13339.5 73 10563.0 74 8345.0 75 6653.5 76 5209.5 77 4122.0 78 3391.5 79 2892.0 80 2486.5 81 2259.5 82 2083.0 83 1876.5 84 1656.5 85 1471.5 86 1229.0 87 938.5 88 709.0 89 543.0 90 418.0 91 331.5 92 244.0 93 169.5 94 133.0 95 94.0 96 65.0 97 45.5 98 35.0 99 26.5 100 10.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.12234014500575581 2 0.11022635907282152 3 0.10546773257206087 4 0.09309126237369181 5 0.09796102452521334 6 0.08764561548640942 7 0.09701131987325685 8 0.09367725035043091 9 0.08824170670412679 10-11 0.07918920278760543 12-13 0.07084392573956229 14-15 0.07891136366070327 16-17 0.06955071089434496 18-19 0.06206926094994309 20-21 0.057527854130215016 22-23 0.05066775350597617 24-25 0.047662039314943686 26-27 0.04472704781075902 28-29 0.04715687726603066 30-31 0.03868025808527015 32-33 0.05084456022309572 34-35 0.06051336183929098 36-37 0.05146590954325874 38-39 0.05333500912423693 40-41 0.053784603347769515 42-43 0.048798653924997985 44-45 0.04868751827423712 46-47 0.04852081479809582 48-49 0.04871277637668277 50-51 0.04989485557113924 52-53 0.046485011740976336 54-55 0.04680326383179154 56-57 0.047252858055324136 58-59 0.049642274546682734 60-61 0.04530293254651987 62-63 0.054400901047443405 64-65 0.05023331414391097 66-67 0.04484323508200902 68-69 0.04291351605516127 70-71 0.05081930212065008 72-73 0.044944267491791624 74-75 0.04700532865135675 76-77 0.042610418825813456 78-79 0.04761152311005238 80-81 0.049404848383693606 82-83 0.039220781477607075 84-85 0.04928866111244362 86-87 0.05166797436282395 88-89 0.047217496711900224 90-91 0.04329238759184604 92-93 0.04093833244391135 94-95 0.044535086232172075 96-97 0.031607989400487826 98-99 0.032971926932552985 100-101 0.024470049649346817 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 9897814.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.18899031192798 #Duplication Level Percentage of deduplicated Percentage of total 1 89.86067696091796 44.201559684538836 2 5.668946745402496 5.576995330768781 3 1.699448890896354 2.5078252508975254 4 0.8447940377852108 1.6621826296076503 5 0.4773994800275647 1.174139919899767 6 0.32100683831583215 0.947400135598806 7 0.21394289245362363 0.7366544404944999 8 0.16119362017707 0.6343161136987596 9 0.11448777434363658 0.5068384220721003 >10 0.6178693786820939 4.944113348804147 >50 0.007677443369433521 0.260380125449199 >100 0.006925298203283616 0.8676881786551192 >500 0.002471000042758344 0.8735371325507701 >1k 0.002633032818887299 2.4634982429478796 >5k 4.0508197213650764E-4 1.4190196212219532 >10k+ 1.2152459164095228E-4 31.22385142279418 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 1511785 15.273928162319478 No Hit AAGCAGTGGTATCAACGCAGAGTATTTTTTTTTTTTTTTTTTTTTTTTTT 31750 0.3207779010597694 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 23489 0.23731502733835974 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 3.030972293478136E-5 0.0 0.0 0.0 0.0 2 5.0516204891302264E-5 0.0 0.0 0.0 0.0 3 6.061944586956272E-5 0.0 0.0 0.0 0.0 4 6.061944586956272E-5 0.0 0.0 0.0 0.0 5 7.072268684782317E-5 0.0 0.0 0.0 0.0 6 8.082592782608362E-5 0.0 0.0 0.0 0.0 7 9.092916880434407E-5 0.0 0.0 0.0 0.0 8 9.092916880434407E-5 0.0 0.0 0.0 0.0 9 1.0103240978260453E-4 0.0 0.0 0.0 0.0 10-11 1.2629051222825567E-4 0.0 0.0 0.0 0.0 12-13 1.6165185565216724E-4 0.0 0.0 0.0 5.051620489130226E-6 14-15 1.717550966304277E-4 0.0 0.0 0.0 2.0206481956520906E-5 16-17 1.919615785869486E-4 0.0 0.0 0.0 2.0206481956520906E-5 18-19 2.121680605434695E-4 0.0 0.0 0.0 3.030972293478136E-5 20-21 2.3742616298912063E-4 0.0 0.0 0.0 3.030972293478136E-5 22-23 2.4752940396738105E-4 0.0 0.0 0.0 3.030972293478136E-5 24-25 2.929939883695531E-4 0.0 0.0 0.0 3.030972293478136E-5 26-27 3.4856181374998563E-4 0.0 0.0 1.0103240978260453E-5 3.030972293478136E-5 28-29 3.8392315717389715E-4 0.0 0.0 2.0206481956520906E-5 3.030972293478136E-5 30-31 4.2938774157606926E-4 0.0 0.0 2.0206481956520906E-5 3.030972293478136E-5 32-33 4.6474908499998083E-4 0.0 0.0 2.0206481956520906E-5 3.030972293478136E-5 34-35 5.051620489130226E-4 0.0 0.0 2.0206481956520906E-5 3.030972293478136E-5 36-37 5.657814947825854E-4 0.0 0.0 2.0206481956520906E-5 4.546458440217204E-5 38-39 6.112460791847574E-4 0.0 0.0 2.0206481956520906E-5 6.061944586956272E-5 40-41 7.122784889673618E-4 0.0 0.0 2.0206481956520906E-5 7.072268684782317E-5 42-43 7.627946938586641E-4 0.0 0.0 2.0206481956520906E-5 7.072268684782317E-5 44-45 8.335173807064874E-4 0.0 0.0 3.030972293478136E-5 7.072268684782317E-5 46-47 8.890852060869198E-4 0.0 0.0 4.041296391304181E-5 7.57743073369534E-5 48-49 9.547562724456127E-4 0.0 0.0 4.041296391304181E-5 1.0103240978260453E-4 50-51 0.0010204273388043058 0.0 0.0 4.041296391304181E-5 1.1113565076086498E-4 52-53 0.0010810467846738684 0.0 0.0 4.041296391304181E-5 1.1113565076086498E-4 54-55 0.001141666230543431 0.0 0.0 4.041296391304181E-5 1.1113565076086498E-4 56-57 0.0012426986403260357 0.0 0.0 5.556782538043249E-5 1.1113565076086498E-4 58-59 0.0013689891525542914 0.0 0.0 6.061944586956272E-5 1.1113565076086498E-4 60-61 0.001540744249184719 0.0 0.0 6.061944586956272E-5 1.1113565076086498E-4 62-63 0.0016367250384781932 1.0103240978260453E-5 0.0 6.061944586956272E-5 1.1618727124999521E-4 64-65 0.0017983768941303604 1.515486146739068E-5 0.0 6.061944586956272E-5 1.2123889173912543E-4 66-67 0.0019903384727173092 2.0206481956520906E-5 0.0 6.567106635869294E-5 1.2629051222825567E-4 68-69 0.0021317838464129553 2.0206481956520906E-5 0.0 7.072268684782317E-5 1.4144537369564634E-4 70-71 0.0022934357020651225 2.5258102445651132E-5 0.0 7.072268684782317E-5 1.56600235163037E-4 72-73 0.002470242419184681 3.030972293478136E-5 0.0 7.072268684782317E-5 1.717550966304277E-4 74-75 0.0027076685821738012 3.030972293478136E-5 0.0 7.57743073369534E-5 1.8185833760868815E-4 76-77 0.0029653012271194427 3.030972293478136E-5 0.0 9.092916880434407E-5 1.919615785869486E-4 78-79 0.003197675769619433 3.030972293478136E-5 0.0 9.092916880434407E-5 1.919615785869486E-4 80-81 0.003505824619456377 4.041296391304181E-5 0.0 9.092916880434407E-5 1.919615785869486E-4 82-83 0.003955418842988967 5.0516204891302264E-5 0.0 9.092916880434407E-5 2.071164400543393E-4 84-85 0.004536355199238943 6.061944586956272E-5 0.0 9.092916880434407E-5 2.2227130152172996E-4 86-87 0.005278943411141086 6.061944586956272E-5 0.0 9.092916880434407E-5 2.2227130152172996E-4 88-89 0.00647617746706495 6.061944586956272E-5 0.0 9.092916880434407E-5 2.2227130152172996E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AAGCAGT 312670 0.0 92.11184 1 AGTGGTA 313690 0.0 91.25902 5 AGCAGTG 315810 0.0 91.00421 2 GGTATCA 316235 0.0 90.75773 8 GTATCAA 317000 0.0 90.69667 9 TGGTATC 315505 0.0 90.65872 7 GTGGTAT 316565 0.0 90.50166 6 GCAGTGG 317315 0.0 90.42141 3 CAGTGGT 318120 0.0 89.93843 4 GTATCGA 690 0.0 46.138508 9 ATCAACG 316810 0.0 45.862747 10-11 CAACGCA 317160 0.0 45.793793 12-13 TCAACGC 316910 0.0 45.715977 12-13 ACGCAGA 318415 0.0 45.650375 14-15 AACGCAG 318585 0.0 45.52833 14-15 TATCAAC 318130 0.0 45.47604 10-11 CGCAGAG 319110 0.0 45.384354 16-17 AGAGTAC 311350 0.0 45.341877 18-19 GAGTACT 180775 0.0 45.230843 20-21 CAGAGTA 316045 0.0 45.230442 18-19 >>END_MODULE