FastQCFastQC Report
Sat 18 Jun 2016
SRR578609_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR578609_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9897814
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT270930.27372710782401044No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT189710.19166858459857902No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT102620.10367945891890877No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT120300.029.563151
AGTGGTA144500.023.8858225
GGTATCA154200.022.2909138
GTATCAA156400.021.8862889
AGCAGTG164950.021.8455542
CGTCACG7650.021.7210223
TGGTATC163850.021.0070557
GTGGTAT171250.020.293376
ATCAACG141300.019.87399110-11
GCAGTGG180300.019.8013923
CAACGCA143750.019.5187112-13
CGCGTAG4500.018.99124354-55
ACTCGCG4600.018.06460450-51
CAGTGGT196550.017.8986784
CTCGCGT4650.017.86932252-53
ACGCAGA164350.017.3177314-15
ACGTACG4950.017.26406720-21
CGCTAGT5250.017.18229190-91
AGAGTAC163350.016.8571718-19
TCAACGC139950.015.9779310-11
Produced by FastQC (version 0.11.5)