##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR578609_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 9897814 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.389840625414866 31.0 31.0 34.0 30.0 34.0 2 31.76599812847564 31.0 31.0 34.0 30.0 34.0 3 31.84603125498216 31.0 31.0 34.0 30.0 34.0 4 35.544537207912775 37.0 35.0 37.0 33.0 37.0 5 35.36010385727596 37.0 35.0 37.0 33.0 37.0 6 35.34838510806527 37.0 35.0 37.0 32.0 37.0 7 35.333058491501255 37.0 35.0 37.0 32.0 37.0 8 35.29088918017655 37.0 35.0 37.0 32.0 37.0 9 36.82726953648553 39.0 37.0 39.0 32.0 39.0 10-11 36.82422234849028 39.0 37.0 39.0 32.0 39.0 12-13 36.819740702340944 39.0 37.0 39.0 32.0 39.0 14-15 37.98316456542828 40.0 37.0 41.0 33.0 41.0 16-17 37.89738698868255 40.0 37.0 41.0 32.5 41.0 18-19 37.75867464270394 40.0 36.5 41.0 32.0 41.0 20-21 37.68058623853712 39.5 36.0 41.0 32.0 41.0 22-23 37.45760897305203 39.0 36.0 41.0 32.0 41.0 24-25 37.459891800351066 39.0 36.0 41.0 32.0 41.0 26-27 37.28329401825494 39.0 36.0 41.0 31.5 41.0 28-29 37.12387583763445 39.0 36.0 41.0 31.0 41.0 30-31 36.97345848285288 39.0 35.5 40.5 31.0 41.0 32-33 36.8220815727594 39.0 35.0 40.0 30.0 41.0 34-35 36.660676791865356 38.5 35.0 40.0 30.0 41.0 36-37 36.4869012996203 38.0 35.0 40.0 30.0 41.0 38-39 36.28772176361366 38.0 35.0 40.0 30.0 41.0 40-41 36.105819022260874 38.0 35.0 40.0 30.0 41.0 42-43 36.155010086065474 38.0 35.0 40.0 30.0 41.0 44-45 36.06597734610895 38.0 35.0 40.0 29.5 41.0 46-47 35.893278152125305 38.0 34.5 40.0 29.0 41.0 48-49 35.71380246183652 38.0 34.0 40.0 28.0 41.0 50-51 35.491981259700374 38.0 34.0 40.0 28.0 41.0 52-53 35.4985737759873 38.0 34.0 40.0 28.0 41.0 54-55 35.453926594296476 38.0 34.0 40.0 28.0 41.0 56-57 35.26656562752139 38.0 34.0 40.0 27.5 41.0 58-59 35.01817936768664 37.0 34.0 40.0 27.0 41.0 60-61 34.7381602139624 37.0 33.0 40.0 26.5 41.0 62-63 34.4396858740728 36.5 33.0 39.5 26.0 41.0 64-65 34.10353407328123 36.0 33.0 39.0 26.0 41.0 66-67 33.7526861991951 35.5 32.5 39.0 26.0 40.5 68-69 33.37004974027599 35.0 32.0 38.5 26.0 40.0 70-71 32.97374662728558 35.0 32.0 38.0 25.0 40.0 72-73 32.5864764684404 35.0 31.0 37.0 24.0 39.5 74-75 32.191214140819376 35.0 31.0 36.5 24.0 39.0 76-77 30.844596645279452 33.5 29.5 35.0 21.5 38.0 78-79 31.508768552328828 34.0 31.0 36.0 23.0 38.0 80-81 31.40816790454943 34.0 31.0 35.0 23.0 37.0 82-83 31.065992147356983 34.0 31.0 35.0 21.0 37.0 84-85 30.802022345540138 34.0 30.5 35.0 20.0 36.5 86-87 30.503851405977116 34.0 30.0 35.0 20.0 36.0 88-89 30.205003044106505 34.0 30.0 35.0 18.5 36.0 90-91 29.8659807610044 34.0 30.0 35.0 17.5 35.0 92-93 29.494576024564616 34.0 29.0 35.0 12.5 35.0 94-95 29.106711188955458 34.0 29.0 35.0 4.5 35.0 96-97 28.626965711822834 34.0 29.0 35.0 2.0 35.0 98-99 27.799533412125143 33.0 28.0 35.0 2.0 35.0 100-101 26.745053806830477 32.5 25.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.36305417688742736 1101 2 0.23891685984716915 1101 3 0.32229387815302957 1101 4 0.28896479015936194 1101 5 0.3027771256080882 1101 6 0.18340526091940035 1101 7 0.2209608693471381 1101 8 0.19366515588281885 1101 9 0.2877572126980894 1101 10-11 0.29960444969098177 1101 12-13 0.32882474909362713 1101 14-15 0.23717893640320398 1101 16-17 0.32390901334692757 1101 18-19 0.37470201868611497 1101 20-21 0.3370454994041623 1101 22-23 0.4260056863288284 1101 24-25 0.36125215114199705 1101 26-27 0.28209033513796555 1101 28-29 0.3194375693860039 1101 30-31 0.3384837389481845 1101 32-33 0.4154135176307321 1101 34-35 0.3983387116136967 1101 36-37 0.37873371580042203 1101 38-39 0.25529279278144656 1101 40-41 0.2176454997723738 1101 42-43 0.2426015311402665 1101 44-45 0.29849421289333833 1101 46-47 0.18722467355934924 1101 48-49 0.25020629669568706 1101 50-51 0.3409276063233193 1101 52-53 0.25613340478165725 1101 54-55 0.30735200723590594 1101 56-57 0.38513861931232185 1101 58-59 0.37671331958443943 1101 60-61 0.3417344220722569 1101 62-63 0.23789662442123927 1101 64-65 0.1378855193907924 1101 66-67 0.2565764281807361 1101 68-69 0.18070482993592663 1101 70-71 0.2577233791051299 1101 72-73 0.35060900081527535 1101 74-75 0.305739371654731 1101 76-77 0.23144426267817053 1101 78-79 0.2983720020116536 1101 80-81 0.27290179186107366 1101 82-83 0.3899907469949717 1101 84-85 0.3209929431789078 1101 86-87 0.325021788734432 1101 88-89 0.4455669783336482 1101 90-91 0.3515520983416458 1101 92-93 0.3660106983863862 1101 94-95 0.2807761039027561 1101 96-97 0.3148496190016452 1101 98-99 0.7090645757483287 1101 100-101 0.8507924051912603 1102 1 0.26314291007330937 1102 2 0.28979538604888333 1102 3 0.33765891855369645 1102 4 0.3132772287928276 1102 5 0.2929224740462999 1102 6 0.19056799120279777 1102 7 0.3128865650665418 1102 8 0.19275051778759433 1102 9 0.2256265065887817 1102 10-11 0.3279183248741262 1102 12-13 0.3071758574175192 1102 14-15 0.3745568726935531 1102 16-17 0.2128217921049398 1102 18-19 0.3252763534143668 1102 20-21 0.2970804784513419 1102 22-23 0.48952977410238674 1102 24-25 0.34051024698196386 1102 26-27 0.3932444107459858 1102 28-29 0.386057377262226 1102 30-31 0.41740770794685744 1102 32-33 0.4282647652405913 1102 34-35 0.36344338100911955 1102 36-37 0.44040368595784685 1102 38-39 0.3946569992061555 1102 40-41 0.33395699365838993 1102 42-43 0.343749644806131 1102 44-45 0.3924356404846421 1102 46-47 0.36965889066103585 1102 48-49 0.3115000884077759 1102 50-51 0.4312479128259241 1102 52-53 0.46944027506939534 1102 54-55 0.3890609996966816 1102 56-57 0.4539875633390835 1102 58-59 0.5170487000961259 1102 60-61 0.6343061156833301 1102 62-63 0.3756781468855195 1102 64-65 0.39347061132629335 1102 66-67 0.44874228345748435 1102 68-69 0.38582128885932576 1102 70-71 0.36700014371145784 1102 72-73 0.47922888466566604 1102 74-75 0.37568647101715413 1102 76-77 0.2500429607554828 1102 78-79 0.3753449090055483 1102 80-81 0.47148283416089853 1102 82-83 0.5234708026165684 1102 84-85 0.49997576315821846 1102 86-87 0.5358970797512512 1102 88-89 0.3619704386572522 1102 90-91 0.44676894948892354 1102 92-93 0.394384492634817 1102 94-95 0.4218188029304244 1102 96-97 0.6128705789787254 1102 98-99 0.977464382932471 1102 100-101 1.074355947334979 1103 1 0.20205933101423312 1103 2 0.23018290053439472 1103 3 0.2554730889948793 1103 4 0.23504534468209926 1103 5 0.26218028939901217 1103 6 0.028667410538510296 1103 7 0.20277524927561075 1103 8 0.07688374804541098 1103 9 0.16852834468029698 1103 10-11 0.16009802124272454 1103 12-13 0.2393050928925149 1103 14-15 0.30202009649246975 1103 16-17 0.2939730557999667 1103 18-19 0.21734685131718123 1103 20-21 0.37771992884469086 1103 22-23 0.3403850157220205 1103 24-25 0.3074379429826024 1103 26-27 0.17322927437378866 1103 28-29 0.18223261177760008 1103 30-31 0.2331428899507486 1103 32-33 0.20345406184349457 1103 34-35 0.24258643157565274 1103 36-37 0.26341604508500893 1103 38-39 0.25813239352952166 1103 40-41 0.19666204222756534 1103 42-43 0.25013578598788655 1103 44-45 0.18529877217384438 1103 46-47 0.22544061969210105 1103 48-49 0.22238104278753212 1103 50-51 0.12695641061785068 1103 52-53 0.1224224862014438 1103 54-55 0.32302723465501515 1103 56-57 0.24728038804116892 1103 58-59 0.15962863196405408 1103 60-61 0.1991373962653853 1103 62-63 0.29602549551592716 1103 64-65 0.14520844650846243 1103 66-67 0.15132426743209493 1103 68-69 0.36471993890529575 1103 70-71 0.2094659773866212 1103 72-73 0.20876194546163873 1103 74-75 0.23144596130904205 1103 76-77 0.1633748024059507 1103 78-79 0.3399517944528796 1103 80-81 0.2821291271848949 1103 82-83 0.504878894362875 1103 84-85 0.3469599853699741 1103 86-87 0.25732590224925644 1103 88-89 0.3790086306678546 1103 90-91 0.324854313865643 1103 92-93 0.29025553987429475 1103 94-95 0.46558922019742255 1103 96-97 0.5024552768757857 1103 98-99 1.0066868919642822 1103 100-101 0.9173579754018277 1104 1 0.20422984085622176 1104 2 0.2232087053896059 1104 3 0.12066833120573861 1104 4 0.17839228679125085 1104 5 0.13138605723873553 1104 6 0.06640889725473897 1104 7 0.1289710464171847 1104 8 0.071047613162591 1104 9 0.2183459680245079 1104 10-11 0.08381181845376062 1104 12-13 0.15226661892479854 1104 14-15 0.1826959400833843 1104 16-17 0.26176535290600356 1104 18-19 0.10610054401495717 1104 20-21 0.1410322539537603 1104 22-23 0.28251550853376983 1104 24-25 0.19468764203683264 1104 26-27 0.28278502901497404 1104 28-29 0.20312796625890428 1104 30-31 0.1838088905665458 1104 32-33 0.2144102301345825 1104 34-35 0.07680785135026724 1104 36-37 0.011123638105893008 1104 38-39 0.10043478010385343 1104 40-41 0.10511399772455832 1104 42-43 0.07681078834151123 1104 44-45 0.0650150364385027 1104 46-47 0.04169367740426111 1104 48-49 0.1006719723338918 1104 50-51 0.0399005520225586 1104 52-53 -0.017437177788323766 1104 54-55 0.10331146065919228 1104 56-57 0.03072663397230002 1104 58-59 0.03950222338577447 1104 60-61 0.014414411371731717 1104 62-63 -0.062452319149279845 1104 64-65 -0.010567830532963285 1104 66-67 0.021220608891198367 1104 68-69 0.04976666130179552 1104 70-71 0.03259300805164145 1104 72-73 0.10022447583773442 1104 74-75 0.08689922303558006 1104 76-77 0.023757369190750666 1104 78-79 0.09846782365652018 1104 80-81 0.17340316278198387 1104 82-83 0.19795322913190816 1104 84-85 0.14966871509236057 1104 86-87 0.25410193795333313 1104 88-89 0.2407145882680517 1104 90-91 0.1898586881283073 1104 92-93 0.23288701335400575 1104 94-95 0.35034270822565006 1104 96-97 0.297012458954967 1104 98-99 0.6346868802752574 1104 100-101 0.7119569624848232 1105 1 0.2367334897295592 1105 2 0.16097096512801556 1105 3 0.13288307970637447 1105 4 0.16027957623408895 1105 5 0.25186621209090276 1105 6 0.08883328162780657 1105 7 0.2855718631399924 1105 8 0.09859881361904144 1105 9 0.23458515739093855 1105 10-11 0.08593852168110061 1105 12-13 0.13595619438181927 1105 14-15 0.18925533466480005 1105 16-17 0.14664302241120453 1105 18-19 0.20692136143468787 1105 20-21 0.1376805173525426 1105 22-23 0.26167283715641787 1105 24-25 0.22224719347057942 1105 26-27 0.24382432778059382 1105 28-29 0.23696931968843415 1105 30-31 0.17777524245943965 1105 32-33 0.2945997940841636 1105 34-35 0.2554982045989149 1105 36-37 0.1812925653902795 1105 38-39 0.19505838210081095 1105 40-41 0.2811421777207954 1105 42-43 0.24797226648019688 1105 44-45 0.22123241066756094 1105 46-47 0.2226749158500425 1105 48-49 0.254024758006409 1105 50-51 0.2890654034569593 1105 52-53 0.3229121749716555 1105 54-55 0.3494943800591841 1105 56-57 0.2942804549811413 1105 58-59 0.13849667236592467 1105 60-61 0.05487095426308741 1105 62-63 0.18591554311052505 1105 64-65 0.20704292944478908 1105 66-67 0.25038049318094835 1105 68-69 0.20644430978859418 1105 70-71 0.29854411874087106 1105 72-73 0.31383372201022297 1105 74-75 0.27646647696342797 1105 76-77 0.1330074606725411 1105 78-79 0.19900156987720408 1105 80-81 0.34339102128361887 1105 82-83 0.31576028560143143 1105 84-85 0.324409883321529 1105 86-87 0.29428546350395024 1105 88-89 0.3251355697680687 1105 90-91 0.546609254002913 1105 92-93 0.6113675015058355 1105 94-95 0.5124149771302626 1105 96-97 0.6013705645743563 1105 98-99 1.001226238056912 1105 100-101 1.0730923829863386 1106 1 0.16015003285725626 1106 2 0.15048748983668858 1106 3 0.09796065636839302 1106 4 0.10209257244959247 1106 5 0.08748245689005785 1106 6 0.024543227189617767 1106 7 0.06439755517804002 1106 8 0.027762413315620904 1106 9 0.16424058860597768 1106 10-11 0.010996170014450968 1106 12-13 0.04846119768141932 1106 14-15 0.014843464647590565 1106 16-17 0.010147687837552155 1106 18-19 0.11333670973429832 1106 20-21 0.14861219653673174 1106 22-23 0.2645754734623651 1106 24-25 0.1378370277291907 1106 26-27 0.1417098559558454 1106 28-29 0.1880224497411973 1106 30-31 0.21449385322005554 1106 32-33 0.19821584070103881 1106 34-35 0.1853708105559022 1106 36-37 0.17791956731223024 1106 38-39 0.16368195345090442 1106 40-41 0.22579468551791138 1106 42-43 0.16432599706653406 1106 44-45 0.12470307774129452 1106 46-47 0.06134013988431519 1106 48-49 0.16163812274000833 1106 50-51 0.15147625870751824 1106 52-53 0.142982190200037 1106 54-55 0.18402394832689595 1106 56-57 0.20225132769192555 1106 58-59 0.19792999951370405 1106 60-61 0.31258851378601094 1106 62-63 0.3094993484074493 1106 64-65 0.1850105656371852 1106 66-67 0.1574512651261344 1106 68-69 0.2865561910223633 1106 70-71 0.2631372039686539 1106 72-73 0.3102983475065102 1106 74-75 0.34713134867366335 1106 76-77 0.20549675619189856 1106 78-79 0.28967631400072236 1106 80-81 0.4086971956936516 1106 82-83 0.5931101619554191 1106 84-85 0.5263303246073896 1106 86-87 0.5677229832160897 1106 88-89 0.5526414343806785 1106 90-91 0.6026907546280817 1106 92-93 0.5785233771400335 1106 94-95 0.4812354003641026 1106 96-97 0.5669723608764698 1106 98-99 1.0226786663976206 1106 100-101 1.1335945869613155 1107 1 0.16503419116870433 1107 2 0.05719518526900558 1107 3 0.018785995092432728 1107 4 -0.014101934822122075 1107 5 0.0035388648862735295 1107 6 -0.005673525547038594 1107 7 0.0014041584641972804 1107 8 0.045421280659127206 1107 9 0.11619997214069144 1107 10-11 -0.010596719816952316 1107 12-13 0.014668890483491737 1107 14-15 -0.020375510073925795 1107 16-17 0.043570045419521364 1107 18-19 -0.03176857666846189 1107 20-21 0.05893643250214353 1107 22-23 0.08983492421698003 1107 24-25 0.10034998168366371 1107 26-27 0.10071278284625862 1107 28-29 0.04667043084552347 1107 30-31 0.007667316476791086 1107 32-33 0.05469974806428013 1107 34-35 0.18796919485384223 1107 36-37 0.13008845554445259 1107 38-39 0.21168748474086385 1107 40-41 0.11933782393030157 1107 42-43 0.1257961848454201 1107 44-45 0.11882132484569041 1107 46-47 0.02948218315436435 1107 48-49 0.04147473652199096 1107 50-51 0.11749036550962444 1107 52-53 0.12259710867274265 1107 54-55 0.0704926208561858 1107 56-57 0.12994983853894126 1107 58-59 0.07845441081351368 1107 60-61 0.12490750435760845 1107 62-63 0.1329066495546698 1107 64-65 0.17986020370409506 1107 66-67 0.11035625588939979 1107 68-69 0.07514175299240833 1107 70-71 0.10609627942167776 1107 72-73 0.03911796007376722 1107 74-75 0.14694073599364543 1107 76-77 0.04018526776394893 1107 78-79 0.08798506674560969 1107 80-81 0.17736444162403942 1107 82-83 0.2827166933787204 1107 84-85 0.2932002215040903 1107 86-87 0.33646396748105545 1107 88-89 0.29754007504741153 1107 90-91 0.43423397400626484 1107 92-93 0.4833127094170031 1107 94-95 0.3866244741452114 1107 96-97 0.45911483687891774 1107 98-99 0.8642466169362137 1107 100-101 0.9039780102909454 1108 1 0.07343438670017832 1108 2 0.02145888181669875 1108 3 0.0010302912857866886 1108 4 -0.038239113899521726 1108 5 0.05704911433476667 1108 6 0.04277240582477049 1108 7 -0.020939013860768796 1108 8 0.061565426958388514 1108 9 0.016515431653331802 1108 10-11 -0.02451830009926681 1108 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-1.2525303691848393 2316 98-99 -0.9454935545895182 2316 100-101 -1.3227572690960976 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 175.0 3 140.0 4 387.0 5 1185.0 6 2002.0 7 3255.0 8 5198.0 9 7946.0 10 10729.0 11 13925.0 12 17049.0 13 20509.0 14 23619.0 15 26529.0 16 30752.0 17 36168.0 18 42738.0 19 49609.0 20 53219.0 21 54338.0 22 59557.0 23 70818.0 24 88006.0 25 108928.0 26 138289.0 27 173435.0 28 216299.0 29 265485.0 30 323929.0 31 389880.0 32 475629.0 33 589625.0 34 771993.0 35 1116985.0 36 1318344.0 37 1720231.0 38 1403589.0 39 266987.0 40 333.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 17.25159856672897 30.254515906738217 28.36017765113147 24.13370787540135 2 22.68413005134265 30.217712719192342 25.940101521406646 21.158055708058367 3 22.44210691370842 28.691466620811422 26.444364381872603 22.422062083607553 4 22.31766165278772 28.167560912476258 26.947533201060526 22.567244233675492 5 22.91535744840956 28.546376737210544 27.473437434960058 21.064828379419836 6 23.1673463795916 28.587264692801618 25.84585917323496 22.399529754371827 7 23.2633204402273 28.635273953918627 26.784619525397375 21.316786080456698 8 22.488137279605375 29.045443771725754 26.370934026442605 22.095484922226262 9 21.91670558101462 28.718364453698126 27.180316526713096 22.184613438574168 10-11 21.856025269490836 29.16115933205463 26.49007194589133 22.492743452563204 12-13 21.869541092608934 29.301298246259222 26.214369152622996 22.61479150850885 14-15 22.15465960463593 29.39757202954107 26.01290547589599 22.434862889927008 16-17 22.397414701350158 29.038867377961918 26.048469095998183 22.515248824689742 18-19 22.25074479840874 29.249821614624445 26.05761626622044 22.441817320746377 20-21 22.401037239131792 29.10359802679662 26.200350905765657 22.295013828305926 22-23 22.3876054298584 28.99646874414482 26.24338442051522 22.37254140548156 24-25 22.206403353306094 28.992214846631793 26.263076877379188 22.53830492268293 26-27 22.481428454375177 28.76477234392319 26.5255026797361 22.228296521965532 28-29 22.461809771963733 28.905767740749756 26.401146134989737 22.231276352296774 30-31 22.341002137270596 28.9320269950709 26.386852797691606 22.340118069966906 32-33 22.373941153375732 28.98784153808756 26.27812266930483 22.36009463923188 34-35 22.38516554653445 29.00837572063978 26.27310312162438 22.333355611201394 36-37 22.38754737431712 28.96837953768971 26.272165603396648 22.371907484596527 38-39 22.395892664213942 28.984374206729026 26.24463821438211 22.37509491467492 40-41 22.374236702883813 29.040329488532585 26.230989938693742 22.354443869889856 42-43 22.38014493058188 29.058294731788305 26.20398124655981 22.357579091070008 44-45 22.386393108170353 29.067589004328156 26.215420294048673 22.330597593452815 46-47 22.388116329866524 29.105160507904337 26.177928183375826 22.328794978853317 48-49 22.419150303131897 29.10627482998641 26.14003666490703 22.334538201974663 50-51 22.451538902579575 29.092309601970246 26.1216577305742 22.334493764875983 52-53 22.375903407249954 29.19826479520967 26.06322621761642 22.362605579923958 54-55 22.39411341592288 29.21896124940981 25.970664278655303 22.416261056012008 56-57 22.45924076193061 29.277797207045193 25.956433121932914 22.306528909091288 58-59 22.408454780435903 29.366027357594177 25.898667977140725 22.32684988482919 60-61 22.3977789439163 29.36667126700906 25.88545814257572 22.350091646498914 62-63 22.38550697285702 29.414053091351132 25.877709488808026 22.32273044698382 64-65 22.365481717053534 29.449213857979316 25.918769593183168 22.26653483178398 66-67 22.32576024817065 29.469562415248667 25.90423103617298 22.300446300407707 68-69 22.33816303976027 29.505824710517437 25.908856759774572 22.24715548994772 70-71 22.335611354115485 29.556513899745866 25.869953474706612 22.237921271432032 72-73 22.296867747333547 29.57243456800302 25.816875684914415 22.31382199974902 74-75 22.358026373399213 29.612719222004273 25.818794309097477 22.210460095499037 76-77 22.27603457219106 29.722780097853374 25.840263755111557 22.160921574844007 78-79 22.32778379828901 29.749225219814896 25.747470661205707 22.175520320690396 80-81 22.27884929557059 29.804528239513477 25.74078598088181 22.175836484034125 82-83 22.356327921489918 29.861251472266083 25.668187155802947 22.114233450441052 84-85 22.273674704963643 29.910215956456206 25.66672986249326 22.149379476086896 86-87 22.284751803879445 29.96310782186012 25.612413238668886 22.139727135591546 88-89 22.280251999637898 30.000564772426014 25.62857453460327 22.09060869333282 90-91 22.211010303755877 30.016236597961658 25.6152047018189 22.157548396463564 92-93 22.239138107869984 30.077895641148917 25.580638181801557 22.102328069179546 94-95 22.212942881673726 30.137817403458207 25.55120697323246 22.098032741635613 96-97 22.18339004084628 30.183905513174576 25.522023080369483 22.11068136560966 98-99 22.233641967800313 30.201928718678307 25.447338498137157 22.117090815384223 100-101 22.283488969888992 30.16467560822274 25.431473735178827 22.120361686709437 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 303933.0 1 168546.0 2 23805.0 3 13026.5 4 10836.5 5 9462.0 6 9029.5 7 9014.0 8 8402.5 9 7682.0 10 7747.5 11 9780.5 12 20245.0 13 19690.0 14 9743.0 15 9529.0 16 10083.5 17 11179.5 18 13171.5 19 14432.0 20 16471.5 21 20444.5 22 25485.5 23 31451.0 24 38659.0 25 46399.0 26 55229.5 27 64992.0 28 73695.0 29 82796.0 30 95156.5 31 110251.0 32 124348.0 33 138126.0 34 153176.5 35 166914.5 36 182397.5 37 200981.5 38 217789.0 39 228323.5 40 243502.5 41 263275.0 42 278234.5 43 293488.0 44 306501.5 45 320648.5 46 333402.0 47 343247.5 48 357633.0 49 369265.0 50 371447.0 51 370593.0 52 372685.0 53 368675.5 54 351642.5 55 328378.0 56 299217.5 57 268710.0 58 247681.5 59 226199.0 60 195819.0 61 162762.0 62 130061.5 63 100835.5 64 78560.5 65 60398.5 66 47558.5 67 40491.5 68 32365.5 69 24970.0 70 19898.5 71 16673.0 72 14583.5 73 11746.0 74 9124.0 75 7023.0 76 5231.0 77 3792.0 78 2746.5 79 1911.5 80 1343.5 81 1042.0 82 898.0 83 763.0 84 656.0 85 569.5 86 421.5 87 273.5 88 147.5 89 85.5 90 60.5 91 40.5 92 26.5 93 19.5 94 13.5 95 7.0 96 5.0 97 3.0 98 0.5 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.15684271294651525 2 0.0 3 0.0 4 8.890852060869197E-4 5 5.051620489130227E-4 6 8.082592782608362E-5 7 1.8185833760868812E-4 8 0.0 9 8.082592782608362E-5 10-11 2.879423678804229E-4 12-13 0.0 14-15 0.0 16-17 2.0206481956520906E-5 18-19 1.5154861467390677E-5 20-21 0.0 22-23 6.112460791847574E-4 24-25 0.0 26-27 1.1113565076086497E-4 28-29 0.005561834158532379 30-31 0.003813973469293321 32-33 1.6165185565216724E-4 34-35 9.951692363586546E-4 36-37 4.6474908499998083E-4 38-39 0.001096201646141259 40-41 0.002950146365652052 42-43 0.0011113565076086498 44-45 6.567106635869293E-4 46-47 0.008804974512553984 48-49 0.00893631664527137 50-51 0.007855269860597503 52-53 0.01475578344874939 54-55 0.00603163486402149 56-57 8.9413682657605E-4 58-59 0.0012477502608151658 60-61 0.0 62-63 6.061944586956271E-5 64-65 8.032076577717059E-4 66-67 0.001096201646141259 68-69 0.002864268817336838 70-71 0.0018893060629347046 72-73 0.005981118659130188 74-75 0.002298487322554253 76-77 0.001495279664782547 78-79 0.001697344484347756 80-81 1.6165185565216724E-4 82-83 0.002338900286467295 84-85 8.587754831521385E-5 86-87 0.0018943576834238348 88-89 2.2227130152172993E-4 90-91 0.001136614610054301 92-93 0.0015003312852716773 94-95 8.082592782608362E-4 96-97 9.648595134238733E-4 98-99 0.5332541104530758 100-101 2.1721968103259975E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 9897814.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 73.2509703687443 #Duplication Level Percentage of deduplicated Percentage of total 1 90.37411228136716 66.19991420823996 2 5.255566043940174 7.699506251112807 3 1.4928592926259379 3.280601754265415 4 0.7546176948792732 2.211059136293271 5 0.4514452078699453 1.6534399772396489 6 0.30035546004948843 1.3200797342505384 7 0.21401433089843913 1.097373018778974 8 0.1732064472990709 1.0150032271023772 9 0.12719664047229104 0.8385549608015652 >10 0.8386600301033301 11.298167784847237 >50 0.016036194210194026 0.7138065283345749 >100 0.0014724400436409 0.2059219701979723 >500 1.248917034912737E-4 0.05360984936006606 >1k 1.2489170155881014E-4 0.1719296510186375 >5k 8.326113437254009E-5 0.5233821568699673 >10k+ 1.2489170155881014E-4 1.7176497912869415 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 27093 0.27372710782401044 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 18971 0.19166858459857902 No Hit GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT 10262 0.10367945891890877 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 3.030972293478136E-5 0.0 0.0 1.0103240978260453E-5 0.0 2 3.030972293478136E-5 0.0 0.0 1.0103240978260453E-5 0.0 3 7.072268684782317E-5 0.0 0.0 1.0103240978260453E-5 1.0103240978260453E-5 4 8.082592782608362E-5 0.0 0.0 1.0103240978260453E-5 1.0103240978260453E-5 5 8.082592782608362E-5 0.0 0.0 1.0103240978260453E-5 1.0103240978260453E-5 6 9.092916880434407E-5 0.0 0.0 2.0206481956520906E-5 1.0103240978260453E-5 7 1.0103240978260453E-4 0.0 0.0 2.0206481956520906E-5 1.0103240978260453E-5 8 1.0103240978260453E-4 0.0 0.0 2.0206481956520906E-5 1.0103240978260453E-5 9 1.0103240978260453E-4 0.0 0.0 2.0206481956520906E-5 4.041296391304181E-5 10-11 1.6165185565216724E-4 0.0 0.0 2.0206481956520906E-5 4.546458440217204E-5 12-13 1.9701319907607882E-4 0.0 0.0 2.0206481956520906E-5 5.0516204891302264E-5 14-15 2.0206481956520906E-4 0.0 0.0 2.0206481956520906E-5 5.0516204891302264E-5 16-17 2.071164400543393E-4 0.0 0.0 2.0206481956520906E-5 6.061944586956272E-5 18-19 2.4247778347825087E-4 0.0 0.0 3.030972293478136E-5 6.567106635869294E-5 20-21 2.5763264494564153E-4 0.0 0.0 3.030972293478136E-5 7.57743073369534E-5 22-23 2.6268426543477177E-4 0.0 0.0 3.030972293478136E-5 8.587754831521385E-5 24-25 3.435101932608554E-4 0.0 0.0 3.030972293478136E-5 9.092916880434407E-5 26-27 4.3443936206519944E-4 0.0 0.0 4.041296391304181E-5 9.59807892934743E-5 28-29 4.8495556695650173E-4 0.0 0.0 4.546458440217204E-5 1.0103240978260453E-4 30-31 5.152652898912831E-4 0.0 0.0 5.0516204891302264E-5 1.2123889173912543E-4 32-33 5.506266333151946E-4 0.0 0.0 5.0516204891302264E-5 1.363937532065161E-4 34-35 6.061944586956272E-4 0.0 0.0 5.0516204891302264E-5 1.6165185565216724E-4 36-37 6.365041816304085E-4 0.0 0.0 5.0516204891302264E-5 1.8185833760868815E-4 38-39 7.072268684782316E-4 0.0 0.0 5.0516204891302264E-5 1.919615785869486E-4 40-41 8.183625192390966E-4 0.0 0.0 5.0516204891302264E-5 2.0206481956520906E-4 42-43 8.789819651086593E-4 0.0 0.0 5.0516204891302264E-5 2.121680605434695E-4 44-45 9.547562724456127E-4 0.0 0.0 5.0516204891302264E-5 2.121680605434695E-4 46-47 0.001045685441249957 0.0 0.0 5.0516204891302264E-5 2.121680605434695E-4 48-49 0.0011012532666303892 0.0 0.0 5.0516204891302264E-5 2.1721968103259972E-4 50-51 0.001182079194456473 0.0 0.0 5.0516204891302264E-5 2.3742616298912063E-4 52-53 0.0012275437788586449 0.0 0.0 5.0516204891302264E-5 2.4247778347825087E-4 54-55 0.001273008363260817 0.0 0.0 5.556782538043249E-5 2.5763264494564153E-4 56-57 0.0013790923935325518 0.0 0.0 6.061944586956272E-5 2.6268426543477177E-4 58-59 0.0015508474901629794 0.0 0.0 6.061944586956272E-5 2.727875064130322E-4 60-61 0.001722602586793407 0.0 0.0 6.061944586956272E-5 2.8794236788042286E-4 62-63 0.0018640479604890535 0.0 0.0 6.061944586956272E-5 2.9804560885868334E-4 64-65 0.002035803057119481 0.0 5.051620489130226E-6 6.061944586956272E-5 3.030972293478136E-4 66-67 0.0022025065332607783 0.0 1.0103240978260453E-5 6.061944586956272E-5 3.030972293478136E-4 68-69 0.0023186938045107737 0.0 1.0103240978260453E-5 7.072268684782317E-5 3.13200470326074E-4 70-71 0.0025005521421194617 0.0 1.0103240978260453E-5 7.072268684782317E-5 3.13200470326074E-4 72-73 0.00268241047972815 0.0 1.0103240978260453E-5 7.072268684782317E-5 3.13200470326074E-4 74-75 0.0029349915041846615 0.0 1.0103240978260453E-5 7.072268684782317E-5 3.283553317934647E-4 76-77 0.003192624149130303 0.0 1.0103240978260453E-5 7.072268684782317E-5 3.334069522825949E-4 78-79 0.003414895450652033 0.0 1.0103240978260453E-5 7.57743073369534E-5 3.334069522825949E-4 80-81 0.0037786121258694093 0.0 1.0103240978260453E-5 8.082592782608362E-5 3.4856181374998563E-4 82-83 0.004258516072336781 0.0 1.0103240978260453E-5 8.082592782608362E-5 3.637166752173763E-4 84-85 0.004834400808097626 0.0 1.0103240978260453E-5 9.092916880434407E-5 3.738199161956368E-4 86-87 0.005622453604401941 0.0 1.0103240978260453E-5 9.092916880434407E-5 3.839231571738972E-4 88-89 0.006754016593967112 0.0 1.0103240978260453E-5 9.59807892934743E-5 3.9402639815215763E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AAGCAGT 12030 0.0 29.56315 1 AGTGGTA 14450 0.0 23.885822 5 GGTATCA 15420 0.0 22.290913 8 GTATCAA 15640 0.0 21.886288 9 AGCAGTG 16495 0.0 21.845554 2 CGTCACG 765 0.0 21.721022 3 TGGTATC 16385 0.0 21.007055 7 GTGGTAT 17125 0.0 20.29337 6 ATCAACG 14130 0.0 19.873991 10-11 GCAGTGG 18030 0.0 19.801392 3 CAACGCA 14375 0.0 19.51871 12-13 CGCGTAG 450 0.0 18.991243 54-55 ACTCGCG 460 0.0 18.064604 50-51 CAGTGGT 19655 0.0 17.898678 4 CTCGCGT 465 0.0 17.869322 52-53 ACGCAGA 16435 0.0 17.31773 14-15 ACGTACG 495 0.0 17.264067 20-21 CGCTAGT 525 0.0 17.182291 90-91 AGAGTAC 16335 0.0 16.85717 18-19 TCAACGC 13995 0.0 15.97793 10-11 >>END_MODULE