FastQCFastQC Report
Sat 18 Jun 2016
SRR578608_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR578608_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9932826
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT143469714.443996099398095No Hit
AAGCAGTGGTATCAACGCAGAGTATTTTTTTTTTTTTTTTTTTTTTTTTT301730.30377054828102296No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA225840.22736731721667128No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGACAGCACTCGGCCAGCTTC208550.20996038790974492No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT3096150.091.8955461
AGTGGTA3107500.091.095915
AGCAGTG3130800.090.75592
GTATCAA3134700.090.523349
GGTATCA3132800.090.521718
TGGTATC3130050.090.455657
GTGGTAT3136950.090.27626
GCAGTGG3146400.090.079133
CAGTGGT3157200.089.61364
ATCAACG3133100.045.77860310-11
CAACGCA3137350.045.7079212-13
TCAACGC3134000.045.6515112-13
ACGCAGA3147100.045.5860214-15
AACGCAG3144750.045.50084714-15
TATCAAC3149350.045.4052310-11
CGCAGAG3151650.045.35257716-17
AGAGTAC3084300.045.352118-19
CAGAGTA3131400.045.20043618-19
GAGTACT1707850.045.1264920-21
GCAGAGT3163450.045.1218916-17
Produced by FastQC (version 0.11.5)