##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR578608_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9932826
Sequences flagged as poor quality	0
Sequence length	101
%GC	43
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.08725643638578	31.0	31.0	34.0	28.0	34.0
2	31.4374517383069	33.0	31.0	34.0	28.0	34.0
3	31.576637303422007	34.0	31.0	34.0	28.0	34.0
4	34.96664121570236	37.0	35.0	37.0	32.0	37.0
5	34.98782632455255	37.0	35.0	37.0	32.0	37.0
6	35.04054113099333	37.0	35.0	37.0	32.0	37.0
7	34.949340197845004	37.0	35.0	37.0	32.0	37.0
8	35.00629569067252	37.0	35.0	37.0	32.0	37.0
9	36.51658218919772	39.0	37.0	39.0	32.0	39.0
10-11	36.38655217558427	39.0	37.0	39.0	32.0	39.0
12-13	36.469221850860976	39.0	37.0	39.0	32.0	39.0
14-15	37.7496164233623	40.0	38.0	41.0	32.0	41.0
16-17	37.71843113933537	40.0	38.0	41.0	32.0	41.0
18-19	37.625727713341604	40.0	38.0	41.0	32.0	41.0
20-21	37.52816217660513	40.0	38.0	41.0	32.0	41.0
22-23	37.244849401368754	39.5	37.5	41.0	31.5	41.0
24-25	37.22343837493982	40.0	37.0	41.0	31.0	41.0
26-27	37.11553882047264	40.0	37.0	41.0	31.0	41.0
28-29	36.96274177157639	40.0	37.0	41.0	30.0	41.0
30-31	36.71714082175606	40.0	36.5	41.0	30.0	41.0
32-33	36.41879269807002	39.5	36.0	41.0	30.0	41.0
34-35	36.24406684462207	39.0	35.5	41.0	29.0	41.0
36-37	35.929379362932565	39.0	35.0	40.5	27.5	41.0
38-39	34.957539727364605	37.5	35.0	40.0	26.5	41.0
40-41	34.72925806814697	37.0	34.0	40.0	26.0	41.0
42-43	34.42703174303064	37.0	34.0	40.0	24.5	41.0
44-45	34.156346391248576	36.5	33.0	40.0	24.0	41.0
46-47	34.02908975753728	36.5	33.0	40.0	23.5	41.0
48-49	34.079703500292865	37.0	33.0	40.0	23.5	41.0
50-51	33.2484332756861	36.0	32.5	39.5	21.5	40.5
52-53	32.83826465902051	36.5	33.0	39.5	16.0	40.5
54-55	32.28537004473853	37.0	31.5	40.0	4.5	41.0
56-57	31.674771761832936	37.0	31.0	40.0	2.0	41.0
58-59	31.42176818561002	36.0	31.0	40.0	2.0	41.0
60-61	31.18738695311888	36.0	31.0	40.0	2.0	41.0
62-63	30.86076193220338	35.0	31.0	39.0	2.0	41.0
64-65	30.563102534968394	35.0	30.0	39.0	2.0	41.0
66-67	30.226430625080916	35.0	30.0	39.0	2.0	41.0
68-69	29.893790145926246	35.0	29.5	38.0	2.0	40.0
70-71	29.6013424578262	35.0	29.0	37.0	2.0	40.0
72-73	29.281720328132195	35.0	29.0	37.0	2.0	39.0
74-75	28.920816140341127	35.0	29.0	36.0	2.0	39.0
76-77	28.554512230456872	35.0	28.5	36.0	2.0	38.5
78-79	28.2261872401671	35.0	27.5	35.0	2.0	37.0
80-81	27.922432196033636	34.0	27.0	35.0	2.0	37.0
82-83	27.680238433654228	34.0	27.0	35.0	2.0	36.5
84-85	27.421937774808498	34.0	26.0	35.0	2.0	36.0
86-87	27.197335833729497	34.0	26.0	35.0	2.0	36.0
88-89	26.947196850121003	34.0	25.0	35.0	2.0	35.5
90-91	26.71212840132305	34.0	25.0	35.0	2.0	35.0
92-93	26.4147798421114	34.0	24.0	35.0	2.0	35.0
94-95	26.13419725665183	34.0	23.5	35.0	2.0	35.0
96-97	25.8572432457792	34.0	20.5	35.0	2.0	35.0
98-99	25.53611082082783	34.0	18.5	35.0	2.0	35.0
100-101	24.682007265605982	33.0	2.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.5675841398902435
1101	2	0.6181649028205065
1101	3	0.519735282771876
1101	4	0.594277216112026
1101	5	0.5318403745735623
1101	6	0.5248431511081364
1101	7	0.499476701040642
1101	8	0.4986332090026835
1101	9	0.6378125438063904
1101	10-11	0.7128662933787311
1101	12-13	0.7017318480551893
1101	14-15	0.8685672806954869
1101	16-17	0.7995456727092787
1101	18-19	0.8134558863574668
1101	20-21	0.9087111038794333
1101	22-23	0.9287161533033412
1101	24-25	0.8606031475850102
1101	26-27	0.9170520641364206
1101	28-29	1.0636936764576959
1101	30-31	1.1433066695631382
1101	32-33	1.1115720524363013
1101	34-35	1.2153170232855572
1101	36-37	1.1482288255770214
1101	38-39	1.1239321775868305
1101	40-41	1.0854348941329164
1101	42-43	1.115193680990025
1101	44-45	1.2779486863041
1101	46-47	1.1545963768007041
1101	48-49	1.1094595793262059
1101	50-51	1.2460361429151732
1101	52-53	1.068995943757436
1101	54-55	0.9156979479990355
1101	56-57	0.9192614014542535
1101	58-59	0.9332535420387664
1101	60-61	0.8755851922796829
1101	62-63	0.8959363954590707
1101	64-65	0.8655766299505707
1101	66-67	0.8630573795416332
1101	68-69	0.8028263673870306
1101	70-71	0.8822319096190512
1101	72-73	0.8245145384098329
1101	74-75	0.7993543141632777
1101	76-77	0.7538736609844854
1101	78-79	0.7817636942255994
1101	80-81	0.7860062642245218
1101	82-83	0.78859498751331
1101	84-85	0.79538820885071
1101	86-87	0.8056139508761753
1101	88-89	0.77113236183434
1101	90-91	0.8449183893160566
1101	92-93	0.9296638874041712
1101	94-95	0.9905646200457809
1101	96-97	1.0041101024980748
1101	98-99	0.9195815144707247
1101	100-101	1.084312482193262
1102	1	0.4860945647088677
1102	2	0.4298131204895377
1102	3	0.45193201906729286
1102	4	0.3837135627100139
1102	5	0.4735119861864945
1102	6	0.49496791082640357
1102	7	0.49040780142376406
1102	8	0.4335458659705793
1102	9	0.575393860465347
1102	10-11	0.5639012603581435
1102	12-13	0.5722286420585121
1102	14-15	0.7069040385013778
1102	16-17	0.7209812818918877
1102	18-19	0.8112661351673722
1102	20-21	0.7958043330485296
1102	22-23	0.8137902615942636
1102	24-25	0.8228307529290504
1102	26-27	0.8732505991981228
1102	28-29	0.9478906062586603
1102	30-31	0.9439684574204179
1102	32-33	1.0245552820958252
1102	34-35	1.0134012102791061
1102	36-37	1.0871601872179042
1102	38-39	1.0822474920662657
1102	40-41	1.0822598788786522
1102	42-43	1.0484916769026782
1102	44-45	1.1597900053174612
1102	46-47	1.2210089691946209
1102	48-49	1.1534969567634974
1102	50-51	1.14254420493738
1102	52-53	0.9757702082297186
1102	54-55	0.967589836343123
1102	56-57	0.8954750454625291
1102	58-59	0.9087594377991657
1102	60-61	0.8756205802296257
1102	62-63	0.9360513519908586
1102	64-65	0.9161274100030923
1102	66-67	0.889201504565289
1102	68-69	0.8219381879253227
1102	70-71	0.8086410571002638
1102	72-73	0.8300404165829818
1102	74-75	0.7810469193922778
1102	76-77	0.8646173670609407
1102	78-79	0.8448632593012029
1102	80-81	0.8245185442731895
1102	82-83	0.85045806673606
1102	84-85	0.8373146365488786
1102	86-87	0.8225985085198246
1102	88-89	0.8478104498062997
1102	90-91	0.8361856073585088
1102	92-93	0.8908306901329581
1102	94-95	0.979568040035204
1102	96-97	0.9770893190595267
1102	98-99	1.041480697599976
1102	100-101	1.116408823306081
1103	1	0.441841599710159
1103	2	0.47843654651648393
1103	3	0.37463358232381694
1103	4	0.4747429093763387
1103	5	0.4951675126638051
1103	6	0.5205535508018286
1103	7	0.4053379644973063
1103	8	0.4338470471015796
1103	9	0.5345844483034909
1103	10-11	0.5327210344325835
1103	12-13	0.5902324155037562
1103	14-15	0.6576489170743329
1103	16-17	0.7314866678296923
1103	18-19	0.6933768460720984
1103	20-21	0.7173664783292182
1103	22-23	0.8322582835963175
1103	24-25	0.7474540333021622
1103	26-27	0.812210734077432
1103	28-29	0.8109229685254675
1103	30-31	0.8640108117630163
1103	32-33	0.9359885000660313
1103	34-35	0.9106129300240227
1103	36-37	1.0007388507232946
1103	38-39	0.8339419550960017
1103	40-41	0.8964541288004639
1103	42-43	0.7702115278530286
1103	44-45	0.7609191082104374
1103	46-47	0.9881891960793254
1103	48-49	1.0255151590876679
1103	50-51	1.0539210003582298
1103	52-53	0.9404413630140738
1103	54-55	0.8266750114441734
1103	56-57	0.8625536848811244
1103	58-59	0.8360607319676241
1103	60-61	0.8916264552471667
1103	62-63	0.7207097431711063
1103	64-65	0.6639568948060486
1103	66-67	0.6239674439097875
1103	68-69	0.7970661624358293
1103	70-71	0.7385841650415266
1103	72-73	0.7902594560736453
1103	74-75	0.8037304153118789
1103	76-77	0.8385497750892306
1103	78-79	0.8204191387369058
1103	80-81	0.8970714763714582
1103	82-83	0.8526531693847303
1103	84-85	0.8798007806332002
1103	86-87	0.9169292258336803
1103	88-89	0.9589540868317883
1103	90-91	0.9107149255111899
1103	92-93	0.9472202700009973
1103	94-95	0.893268219200813
1103	96-97	0.900312274102518
1103	98-99	0.9334866904665411
1103	100-101	0.9953782797570767
1104	1	0.3594402998164483
1104	2	0.34157233975880175
1104	3	0.36805032016201267
1104	4	0.3862762944770992
1104	5	0.4580595786584283
1104	6	0.4688962130627843
1104	7	0.45097089596333717
1104	8	0.34619109750824606
1104	9	0.49070278272020573
1104	10-11	0.5278635600203927
1104	12-13	0.5770468855578912
1104	14-15	0.6804254698457299
1104	16-17	0.6404325127212616
1104	18-19	0.689318024837668
1104	20-21	0.7279083404636211
1104	22-23	0.7496689626510431
1104	24-25	0.7192346981403119
1104	26-27	0.7574536513373573
1104	28-29	0.7860252068649842
1104	30-31	0.8862783805994852
1104	32-33	0.8789878543701946
1104	34-35	0.9189622047170687
1104	36-37	0.8965400269227572
1104	38-39	0.9509280094630768
1104	40-41	0.898121666518584
1104	42-43	1.0719285502522595
1104	44-45	1.0280799891513013
1104	46-47	1.1537957602504108
1104	48-49	1.0385589174342087
1104	50-51	1.017214598137997
1104	52-53	0.8648085617681858
1104	54-55	0.8586630225267342
1104	56-57	0.839692967653729
1104	58-59	0.9140732113792218
1104	60-61	0.8939609169446321
1104	62-63	0.7901882452189746
1104	64-65	0.6732601064767252
1104	66-67	0.7663001370360014
1104	68-69	0.8052347496220555
1104	70-71	0.7114734975936869
1104	72-73	0.7467776181727181
1104	74-75	0.8074619487212082
1104	76-77	0.8407875007230281
1104	78-79	0.7377338375386095
1104	80-81	0.8208541216747456
1104	82-83	0.8396725077535798
1104	84-85	0.8135906335213434
1104	86-87	0.8625262820864563
1104	88-89	0.8713270838880156
1104	90-91	0.8221681395052443
1104	92-93	0.7358969011965399
1104	94-95	0.6262982516666895
1104	96-97	0.7504486362020621
1104	98-99	1.0268914680182775
1104	100-101	0.9203192632642967
1105	1	0.21952696324773768
1105	2	0.19294104582900218
1105	3	0.2037020627474604
1105	4	0.26608601150301325
1105	5	0.23096111612453996
1105	6	0.22082330122168514
1105	7	0.1650895085968216
1105	8	0.18263620352968246
1105	9	0.12543358285898876
1105	10-11	0.1468233757653934
1105	12-13	0.1365330453714364
1105	14-15	0.0026364801770455415
1105	16-17	0.04817987323205841
1105	18-19	-0.0797607881558946
1105	20-21	0.008049220441911586
1105	22-23	-0.02217046289913327
1105	24-25	-0.028900139447813444
1105	26-27	-0.32173054075828844
1105	28-29	-0.15271161761265972
1105	30-31	-0.312050150645355
1105	32-33	-0.29916015387519934
1105	34-35	-0.19660803546614858
1105	36-37	-0.13230754125594757
1105	38-39	-0.01953461254621658
1105	40-41	-0.014458467821988563
1105	42-43	0.06721406570893862
1105	44-45	0.05042110587369564
1105	46-47	0.08562590435316508
1105	48-49	0.0769867354148559
1105	50-51	0.021380035149519472
1105	52-53	-0.07297121690442765
1105	54-55	-0.08175525632207581
1105	56-57	0.10833330108230399
1105	58-59	0.09894146381666147
1105	60-61	0.008171331622140343
1105	62-63	0.053525018630725896
1105	64-65	-0.022326845159689412
1105	66-67	-0.06015224626452209
1105	68-69	-0.01913331585327782
1105	70-71	-0.001841379737072657
1105	72-73	-0.012319786191820725
1105	74-75	0.06371761063223502
1105	76-77	0.10324376903588117
1105	78-79	0.08515397357355425
1105	80-81	0.175118613277494
1105	82-83	0.1512003832668647
1105	84-85	0.11385821635175475
1105	86-87	0.15122230538194614
1105	88-89	0.24541435099372677
1105	90-91	0.22333671276431488
1105	92-93	0.31322722280220106
1105	94-95	0.3496254352216397
1105	96-97	0.32905715828645654
1105	98-99	0.3211539321495529
1105	100-101	0.29627990676826244
1106	1	-0.08613231504896035
1106	2	-0.027302761865673375
1106	3	-0.061055596009062185
1106	4	-0.09921958424671118
1106	5	-0.11345760877669875
1106	6	-0.12120351170327126
1106	7	-0.17558945948811555
1106	8	-0.15609563133498483
1106	9	-0.13573344059002324
1106	10-11	-0.2994274098865901
1106	12-13	-0.3029657600507747
1106	14-15	-0.2508020059514422
1106	16-17	-0.2891423798841686
1106	18-19	-0.3269921653129515
1106	20-21	-0.12475734064020827
1106	22-23	-0.09729919969937839
1106	24-25	-0.21341596409561703
1106	26-27	-0.2356065286024247
1106	28-29	-0.2908093321075427
1106	30-31	-0.27184123864380894
1106	32-33	-0.18462899635285623
1106	34-35	-0.1804667106369351
1106	36-37	-0.2532244523245808
1106	38-39	-0.29664642258732243
1106	40-41	-0.24244272553819002
1106	42-43	-0.30191517891735487
1106	44-45	-0.2664524922810827
1106	46-47	-0.28420563944476385
1106	48-49	-0.4993305785211888
1106	50-51	-0.5798400894786653
1106	52-53	-0.659633903876518
1106	54-55	-0.5958880216650577
1106	56-57	-0.5627526896172022
1106	58-59	-0.5396198211157284
1106	60-61	-0.6976899115292774
1106	62-63	-0.5297874101419104
1106	64-65	-0.5014747236085952
1106	66-67	-0.7006515934384474
1106	68-69	-0.7811065790050975
1106	70-71	-0.6237126850879449
1106	72-73	-0.6321546628333827
1106	74-75	-0.7282994439628432
1106	76-77	-0.7442807636027489
1106	78-79	-0.6804237933217188
1106	80-81	-0.7070948000821211
1106	82-83	-0.7792967155994077
1106	84-85	-0.8458098287915767
1106	86-87	-0.7598453280104884
1106	88-89	-0.830960901043408
1106	90-91	-0.7463507309946351
1106	92-93	-0.7765256971253969
1106	94-95	-0.7697625898911156
1106	96-97	-0.7746403673430571
1106	98-99	-0.8200762123607106
1106	100-101	-0.9046614740010952
1107	1	-0.4024874581877853
1107	2	-0.4662885534289636
1107	3	-0.4108676051925251
1107	4	-0.29265351295916275
1107	5	-0.37956389465526286
1107	6	-0.3479506067240763
1107	7	-0.30905161479472554
1107	8	-0.2797097606428025
1107	9	-0.37645586504907413
1107	10-11	-0.3420508192975831
1107	12-13	-0.41332929482774006
1107	14-15	-0.6269743215932948
1107	16-17	-0.49379607569867545
1107	18-19	-0.49129060213279274
1107	20-21	-0.357494760440467
1107	22-23	-0.468629678369922
1107	24-25	-0.40923630091579355
1107	26-27	-0.32858220661445614
1107	28-29	-0.419597416600638
1107	30-31	-0.4101265711593811
1107	32-33	-0.3666890581946518
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2308	50-51	-2.547670475913076
2308	52-53	-2.4479281249871505
2308	54-55	-2.2055554697687754
2308	56-57	-2.174105879208522
2308	58-59	-2.0440624901453397
2308	60-61	-2.0333991321125247
2308	62-63	-2.098586619566902
2308	64-65	-2.025153160743372
2308	66-67	-2.1996903983090235
2308	68-69	-2.0305265608096015
2308	70-71	-2.028682423332576
2308	72-73	-2.006664904347037
2308	74-75	-1.7836476595181985
2308	76-77	-1.6788986728190736
2308	78-79	-1.7669288436687687
2308	80-81	-2.38299471114696
2308	82-83	-2.163541589773306
2308	84-85	-1.9257593262545782
2308	86-87	-1.7742470253520537
2308	88-89	-1.7129910025544355
2308	90-91	-1.9234245124212848
2308	92-93	-1.986672958557648
2308	94-95	-2.082996872994496
2308	96-97	-2.009198795558177
2308	98-99	-2.031327092665567
2308	100-101	-2.158602057354134
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	87265.0
3	15195.0
4	13446.0
5	13155.0
6	15101.0
7	18478.0
8	22717.0
9	26050.0
10	29946.0
11	35991.0
12	34763.0
13	38218.0
14	42165.0
15	49096.0
16	60194.0
17	79745.0
18	118012.0
19	196227.0
20	322219.0
21	240770.0
22	123360.0
23	112302.0
24	121649.0
25	125080.0
26	129541.0
27	142588.0
28	162519.0
29	188579.0
30	223818.0
31	268002.0
32	330028.0
33	418257.0
34	561329.0
35	856367.0
36	1444125.0
37	1677144.0
38	1363003.0
39	226190.0
40	192.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	10.998869627633868	49.4287801950542	21.78486018214487	17.787489995167057
2	16.534072301297392	50.86139816097746	16.809532686736365	15.79499685098879
3	47.298377321555854	18.894542123279873	17.464997941499906	16.34208261366437
4	16.412031923501893	17.53989505968205	18.453320455786972	47.59475256102908
5	16.67917612685147	48.5661579150322	19.32113582875652	15.43353012935981
6	47.54789043673243	18.508040938027552	17.414105690794983	16.52996293444503
7	17.43546767161705	18.012350322611304	49.1756316714012	15.376550334370448
8	47.029081088819936	18.76600769748813	18.251848433162365	15.953062780529564
9	46.53418422703888	18.477960561556014	18.87385932424149	16.113995887163618
10-11	16.025930337289417	33.786357746446114	33.68043579833609	16.50727611792838
12-13	15.888254701689428	18.89614875394525	33.488694009199094	31.726902535166225
14-15	16.207586960669655	49.24728984976809	17.997260272593312	16.547862916968942
16-17	31.540994403862534	18.60177961490556	18.169407785199816	31.687818196032087
18-19	16.36237918396757	33.89927499557777	18.17413012909696	31.564215691357706
20-21	31.628082504946608	33.84367898692923	18.32341410318862	16.204824404935543
22-23	31.456678198340597	18.55610522222488	33.651837412206795	16.335379167227728
24-25	16.19928161725971	33.54560466900959	18.849198269055503	31.40591544467519
26-27	16.471098316525843	24.899060414144266	42.19657264498966	16.433268624340233
28-29	29.612695800187876	18.3550435907443	35.70307199861306	16.32918861045476
30-31	26.810619309718593	20.036823143548087	36.11521876439899	17.037338782334334
32-33	20.206741322360394	22.419088516741354	38.034853015751146	19.339317145147103
34-35	19.555061088376156	21.48397641417731	38.82515584827583	20.135806649170704
36-37	19.602361091136565	21.38263298982596	38.900628076901526	20.114377842135948
38-39	19.96412711090246	21.387816980508724	38.56653219828644	20.081523710302385
40-41	19.906406157327503	21.224700832852502	38.38247625897673	20.48641675084327
42-43	20.47424953337226	21.47286486503901	38.2919161938137	19.760969407775033
44-45	19.885469870778582	21.60369867078704	38.173689431157456	20.337142027276915
46-47	20.237909584310366	21.431429226406497	38.170555043163894	20.160106146119247
48-49	20.060889468082234	21.521596385554297	38.36924978666185	20.04826435970162
50-51	20.16817213795381	21.300911437915325	37.95727258792137	20.57364383620949
52-53	20.643956194608197	22.21916506052273	36.69582086723708	20.441057877631994
54-55	21.31612525075602	22.9982780458227	35.06355594198696	20.622040761434317
56-57	21.678329291230956	23.91687280380784	33.68518776119945	20.719610143761756
58-59	21.62965833735671	24.84606181314943	32.79785489442295	20.726424955070915
60-61	21.532169448988338	25.11694055510444	32.45223484882461	20.89865514708261
62-63	21.280935792657115	25.2588366903796	32.31606939024988	21.144158126713407
64-65	21.051261113001466	24.867089470043073	32.69458436205365	21.387065054901814
66-67	21.06035477741351	24.30186122796463	33.67003677540634	20.96774721921552
68-69	20.900295725116273	23.927201317380543	34.18841928952381	20.984083667979377
70-71	20.766504552784568	23.913117213633655	34.63143715748153	20.688941076100253
72-73	20.45516026903476	23.71239199926787	34.5386704203275	21.293777311369873
74-75	20.97581628596093	23.904337594594768	34.020476249776806	21.099369869667502
76-77	21.29161809112092	24.49918171311522	33.106728097406126	21.10247209835773
78-79	21.179193667638856	24.68701674306864	32.8931362638547	21.240653325437812
80-81	21.11154403125493	25.34819800770486	32.27653717873951	21.2637207823007
82-83	21.94614403237514	25.42859147124793	31.58729776370669	21.03796673267023
84-85	21.837256116054522	25.883446337039963	30.999663241663367	21.27963430524215
86-87	21.565257096318007	26.208447947231072	30.90714737138128	21.319147585069633
88-89	21.373784856244967	25.815626834111505	30.90390760054295	21.906680709100577
90-91	21.406447321945546	25.76490856761303	31.021746642452435	21.806897467988986
92-93	21.55630602802874	26.08001066275161	31.03367341522258	21.330009893997065
94-95	21.54991176639478	25.890239845270678	30.458760803215945	22.101087585118595
96-97	21.449015483292527	25.663337049936587	30.86288617736163	22.024761289409252
98-99	21.88229411400001	26.04016277218799	30.278721916731733	21.79882119708026
100-101	22.09933941559209	25.770604107250904	30.11503336690899	22.015023110248016
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	24795.0
1	14148.5
2	2519.0
3	1294.5
4	1048.5
5	952.5
6	1127.5
7	1492.0
8	1359.0
9	1494.0
10	2690.5
11	15113.0
12	276004.0
13	300562.5
14	67034.5
15	59116.0
16	62106.0
17	66133.5
18	69953.5
19	72068.5
20	72445.5
21	71707.5
22	70123.5
23	69793.5
24	71197.0
25	73502.5
26	76827.5
27	81754.5
28	86040.0
29	89646.5
30	95770.0
31	104018.5
32	111623.0
33	119781.0
34	134866.0
35	146547.0
36	154255.5
37	165757.0
38	176692.5
39	187125.5
40	200367.5
41	216984.0
42	231777.5
43	244610.5
44	257961.5
45	271370.0
46	280200.5
47	291065.5
48	305072.5
49	317005.5
50	325991.5
51	330115.0
52	338173.0
53	348094.5
54	338116.5
55	319090.0
56	307581.0
57	291085.5
58	294034.5
59	261622.5
60	196938.5
61	161817.5
62	129838.5
63	101443.5
64	79676.5
65	64225.0
66	51815.5
67	41695.0
68	32096.0
69	24100.0
70	18848.0
71	15236.5
72	12563.5
73	9837.5
74	7583.0
75	5650.0
76	4153.5
77	3206.5
78	2529.0
79	1967.0
80	1574.0
81	1418.5
82	1277.0
83	1114.5
84	931.0
85	754.5
86	572.0
87	408.0
88	298.5
89	211.5
90	160.0
91	135.0
92	101.0
93	70.5
94	60.5
95	42.0
96	20.5
97	17.5
98	15.5
99	9.5
100	4.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05158652733874529
2	0.09137379432600551
3	0.08178941219749546
4	0.05505985909750156
5	0.08393381702246672
6	0.0015504147560825086
7	0.0013087916772125073
8	0.004983476001693778
9	0.010007222516532556
10-11	0.00991158004781318
12-13	0.017532774660504475
14-15	0.011889869006061317
16-17	0.0017265982510918847
18-19	5.939900688887534E-4
20-21	1.5604823843687587E-4
22-23	3.8760368902062716E-4
24-25	9.916613861956306E-4
26-27	0.0015302794995100085
28-29	0.0015655161985118839
30-31	0.004887833532974402
32-33	2.718259637287515E-4
34-35	0.002506839443276264
36-37	4.580770870243776E-4
38-39	0.0025722790271368893
40-41	0.003372655475893769
42-43	0.001233284465065632
44-45	6.896325376081288E-4
46-47	0.0010369657134837557
48-49	0.0023960955321275135
50-51	0.0044649931449519
52-53	0.12671116961074322
54-55	0.13664288491512888
56-57	0.1232881759934182
58-59	0.11714692273880566
60-61	0.10547854155504184
62-63	0.10958110008168873
64-65	0.11189162077338312
66-67	0.12010177164082006
68-69	0.132298703309612
70-71	0.12000109535795754
72-73	0.11209297333910813
74-75	0.11703114501351378
76-77	0.12796962314652446
78-79	0.14220021572913893
80-81	0.13199667446102448
82-83	0.11215337910882561
84-85	0.11844564678773191
86-87	0.11916044839605568
88-89	0.1154656288150019
90-91	0.1257446772952632
92-93	0.14281434105460017
94-95	0.15221750587395771
96-97	0.15270578584584085
98-99	0.135318991795487
100-101	0.12527653257995258
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	9932826.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.82879364740086
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.58431442749959	44.63878317651753
2	5.905258592005523	5.885038236311683
3	1.766233949964167	2.640279210773947
4	0.8448704284958743	1.6839549696124827
5	0.4699577418767463	1.1708713671487436
6	0.31278245340744376	0.9351343396420372
7	0.20864772981149396	0.7277665271642907
8	0.16151991907057703	0.6438674173850136
9	0.12263159985788723	0.5499526215572366
>10	0.6038263171651456	4.9249457284255955
>50	0.007179391194145228	0.23865398366226817
>100	0.007676425139789621	0.9293243091463439
>500	0.0023260671874083235	0.8033444985696345
>1k	0.00220364258113221	2.1004448028071163
>5k	4.080819594689279E-4	1.457334055262384
>10k+	1.6323278378757114E-4	30.67030475601377
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1434697	14.443996099398095	No Hit
AAGCAGTGGTATCAACGCAGAGTATTTTTTTTTTTTTTTTTTTTTTTTTT	30173	0.30377054828102296	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	22584	0.22736731721667128	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGACAGCACTCGGCCAGCTTC	20855	0.20996038790974492	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.0135256572500113E-5	0.0	0.0	0.0	0.0
2	2.0135256572500113E-5	0.0	0.0	0.0	0.0
3	3.020288485875017E-5	0.0	0.0	0.0	0.0
4	4.0270513145000225E-5	0.0	0.0	0.0	0.0
5	5.033814143125028E-5	0.0	0.0	0.0	0.0
6	6.040576971750034E-5	0.0	0.0	0.0	1.0067628286250056E-5
7	7.047339800375039E-5	0.0	0.0	0.0	1.0067628286250056E-5
8	7.047339800375039E-5	0.0	0.0	1.0067628286250056E-5	2.0135256572500113E-5
9	7.047339800375039E-5	0.0	0.0	1.0067628286250056E-5	3.020288485875017E-5
10-11	9.060865457625051E-5	0.0	0.0	1.0067628286250056E-5	3.020288485875017E-5
12-13	1.2081153943500068E-4	0.0	0.0	1.0067628286250056E-5	3.5236699001875194E-5
14-15	1.7114968086625096E-4	0.0	0.0	1.5101442429375084E-5	4.0270513145000225E-5
16-17	2.1142019401125116E-4	0.0	0.0	2.0135256572500113E-5	4.0270513145000225E-5
18-19	2.315554505837513E-4	0.0	0.0	2.516907071562514E-5	4.0270513145000225E-5
20-21	2.7685977787187657E-4	0.0	0.0	3.020288485875017E-5	4.0270513145000225E-5
22-23	3.1209647687375174E-4	0.0	0.0	3.020288485875017E-5	4.0270513145000225E-5
24-25	3.2719791930312683E-4	0.0	0.0	3.020288485875017E-5	4.0270513145000225E-5
26-27	3.52366990018752E-4	0.0	0.0	3.020288485875017E-5	4.0270513145000225E-5
28-29	3.8760368902062716E-4	0.0	0.0	3.020288485875017E-5	4.0270513145000225E-5
30-31	4.3794183045187747E-4	0.0	0.0	3.020288485875017E-5	4.0270513145000225E-5
32-33	4.631109011675026E-4	0.0	0.0	3.020288485875017E-5	4.530432728812526E-5
34-35	4.983476001693779E-4	0.0	0.0	4.530432728812526E-5	5.033814143125028E-5
36-37	5.38618113314378E-4	0.0	0.0	5.033814143125028E-5	5.033814143125028E-5
38-39	6.090915113181284E-4	0.0	0.0	5.033814143125028E-5	5.033814143125028E-5
40-41	6.644634668925037E-4	0.0	0.0	5.033814143125028E-5	5.033814143125028E-5
42-43	7.500383073256291E-4	0.0	0.0	5.033814143125028E-5	5.033814143125028E-5
44-45	7.903088204706294E-4	0.0	0.0	5.033814143125028E-5	5.033814143125028E-5
46-47	8.406469619018798E-4	0.0	0.0	5.033814143125028E-5	5.033814143125028E-5
48-49	8.859512891900049E-4	0.0	0.0	5.033814143125028E-5	5.033814143125028E-5
50-51	9.211879881918801E-4	0.0	0.0	5.033814143125028E-5	5.033814143125028E-5
52-53	9.815937579093804E-4	0.0	0.0	5.033814143125028E-5	6.040576971750034E-5
54-55	0.001047033341770006	0.0	0.0	6.040576971750034E-5	6.040576971750034E-5
56-57	0.001082270040771881	0.0	0.0	6.040576971750034E-5	6.040576971750034E-5
58-59	0.0011779125094912566	0.0	0.0	6.040576971750034E-5	6.040576971750034E-5
60-61	0.0012836226064968821	0.0	0.0	6.040576971750034E-5	7.047339800375039E-5
62-63	0.0014296032166475079	0.0	0.0	6.040576971750034E-5	7.047339800375039E-5
64-65	0.001605786711656884	0.0	0.0	6.040576971750034E-5	7.047339800375039E-5
66-67	0.0017316320652350095	0.0	0.0	6.040576971750034E-5	7.047339800375039E-5
68-69	0.0018625112329562605	0.0	0.0	6.040576971750034E-5	7.047339800375039E-5
70-71	0.0020588299845381366	0.0	0.0	6.543958386062536E-5	7.047339800375039E-5
72-73	0.0022400472936906376	0.0	0.0	8.054102629000045E-5	8.557484043312548E-5
74-75	0.002542076142278139	0.0	0.0	9.06086545762505E-5	9.06086545762505E-5
76-77	0.002879341689867516	0.0	0.0	9.06086545762505E-5	9.06086545762505E-5
78-79	0.0032015057950275177	0.0	0.0	9.06086545762505E-5	9.06086545762505E-5
80-81	0.0035186360860443946	0.0	0.0	9.06086545762505E-5	1.1074391114875061E-4
82-83	0.0039062397750650214	0.0	0.0	1.0067628286250056E-4	1.1074391114875061E-4
84-85	0.004434790260093149	1.0067628286250056E-5	0.0	1.0571009700562558E-4	1.2081153943500068E-4
86-87	0.005064017027983779	1.0067628286250056E-5	0.0	1.1074391114875061E-4	1.2081153943500068E-4
88-89	0.006000306458605033	1.0067628286250056E-5	0.0	1.258453535781257E-4	1.2081153943500068E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGCAGT	309615	0.0	91.895546	1
AGTGGTA	310750	0.0	91.09591	5
AGCAGTG	313080	0.0	90.7559	2
GTATCAA	313470	0.0	90.52334	9
GGTATCA	313280	0.0	90.52171	8
TGGTATC	313005	0.0	90.45565	7
GTGGTAT	313695	0.0	90.2762	6
GCAGTGG	314640	0.0	90.07913	3
CAGTGGT	315720	0.0	89.6136	4
ATCAACG	313310	0.0	45.778603	10-11
CAACGCA	313735	0.0	45.70792	12-13
TCAACGC	313400	0.0	45.65151	12-13
ACGCAGA	314710	0.0	45.58602	14-15
AACGCAG	314475	0.0	45.500847	14-15
TATCAAC	314935	0.0	45.40523	10-11
CGCAGAG	315165	0.0	45.352577	16-17
AGAGTAC	308430	0.0	45.3521	18-19
CAGAGTA	313140	0.0	45.200436	18-19
GAGTACT	170785	0.0	45.12649	20-21
GCAGAGT	316345	0.0	45.12189	16-17
>>END_MODULE