FastQCFastQC Report
Sat 18 Jun 2016
SRR578608_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR578608_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9932826
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2307472.3230750241673417No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT287880.2898268831045666No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT191220.19251318808967358No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT110090.11083451980332687No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT106730.10745179669914685No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT121500.031.5002821
AGTGGTA147750.025.3863685
GGTATCA154150.024.362778
GTATCAA157050.023.8501159
TGGTATC165550.022.627837
AGCAGTG173000.022.1498872
GTGGTAT173500.021.6183246
ATCAACG143500.021.16033710-11
CAACGCA145600.020.87142412-13
GCAGTGG189400.020.005383
CGTCACG6950.019.7876033
CAGTGGT203350.018.67864
ACGCAGA165750.018.34841214-15
CGCGTAG3750.018.33125354-55
AGAGTAC168850.017.5212118-19
AGTACTT131400.016.43613820-21
TCAACGC144450.016.14741310-11
AACGCAG150750.015.53549212-13
GCAGAGT201300.015.23761616-17
ACTCGCG4550.015.10817550-51
Produced by FastQC (version 0.11.5)