##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR578608_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9932826
Sequences flagged as poor quality	0
Sequence length	101
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.97417240571817	31.0	31.0	34.0	30.0	34.0
2	32.248372819578236	34.0	31.0	34.0	30.0	34.0
3	32.32425041976976	34.0	31.0	34.0	30.0	34.0
4	35.889701279374066	37.0	35.0	37.0	35.0	37.0
5	35.76322025574595	37.0	35.0	37.0	33.0	37.0
6	35.73361991844013	37.0	35.0	37.0	33.0	37.0
7	35.72394583374359	37.0	35.0	37.0	33.0	37.0
8	35.63957467894837	37.0	35.0	37.0	33.0	37.0
9	37.27280896695462	39.0	37.0	39.0	33.0	39.0
10-11	37.26676637645721	39.0	37.0	39.0	33.0	39.0
12-13	37.2657269441748	39.0	37.0	39.0	33.0	39.0
14-15	38.568924896097045	40.0	38.0	41.0	34.0	41.0
16-17	38.506575419724456	40.0	38.0	41.0	33.5	41.0
18-19	38.399070264595395	40.0	38.0	41.0	33.5	41.0
20-21	38.30499844656495	40.0	38.0	41.0	33.0	41.0
22-23	38.1729520380202	40.0	37.0	41.0	33.0	41.0
24-25	38.08522931943034	40.0	37.0	41.0	33.0	41.0
26-27	37.91227969764093	40.0	37.0	41.0	33.0	41.0
28-29	37.807715548424994	40.0	37.0	41.0	33.0	41.0
30-31	37.698644373716	40.0	37.0	41.0	32.0	41.0
32-33	37.55369101401756	40.0	36.5	41.0	32.0	41.0
34-35	37.40740067328271	40.0	36.0	41.0	31.0	41.0
36-37	37.25874534598714	39.5	36.0	41.0	31.0	41.0
38-39	37.101302791370756	39.0	35.5	41.0	31.0	41.0
40-41	36.934285167182026	39.0	35.0	41.0	31.0	41.0
42-43	36.749522693742946	39.0	35.0	41.0	30.0	41.0
44-45	36.573934195565286	39.0	35.0	40.5	30.0	41.0
46-47	36.645656684210515	39.0	35.0	41.0	30.0	41.0
48-49	36.60386616054686	39.0	35.0	41.0	30.0	41.0
50-51	36.544915465145564	39.0	35.0	41.0	30.0	41.0
52-53	36.51585978653004	39.0	35.0	41.0	30.0	41.0
54-55	36.348453853918315	39.0	35.0	41.0	30.0	41.0
56-57	36.16957288892406	38.5	35.0	41.0	29.5	41.0
58-59	35.93535158070825	38.0	35.0	40.0	29.0	41.0
60-61	35.69038745871518	38.0	34.5	40.0	29.0	41.0
62-63	35.403469969170914	37.0	34.0	40.0	28.0	41.0
64-65	35.08156656524538	37.0	34.0	40.0	28.0	41.0
66-67	34.75778726014127	36.0	34.0	39.0	28.0	41.0
68-69	34.42369462628259	36.0	34.0	39.0	28.0	41.0
70-71	34.0607735401788	35.0	33.0	39.0	27.0	40.5
72-73	33.6468034877486	35.0	33.0	37.5	26.0	40.0
74-75	33.279410663188905	35.0	33.0	37.0	26.0	39.5
76-77	31.86621727794285	34.0	30.5	36.0	24.5	39.0
78-79	32.48840319965335	35.0	32.0	36.0	26.0	39.0
80-81	32.45982160565382	35.0	32.5	36.0	26.0	37.5
82-83	32.2060787131477	35.0	32.0	35.5	25.5	37.0
84-85	31.941708935604026	35.0	32.0	35.0	25.0	37.0
86-87	31.681662701027882	35.0	32.0	35.0	25.0	36.0
88-89	31.44354119361398	35.0	32.0	35.0	24.0	36.0
90-91	31.213112260297322	35.0	32.0	35.0	24.0	36.0
92-93	30.97431783260877	35.0	32.0	35.0	23.0	35.5
94-95	30.717389743865443	34.0	31.0	35.0	20.0	35.0
96-97	30.114860161649865	34.0	31.0	35.0	11.5	35.0
98-99	30.06748416815114	34.0	31.0	35.0	9.5	35.0
100-101	29.464524597531458	34.0	30.5	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.4607513726080761
1101	2	0.4618117410124398
1101	3	0.4259322640626877
1101	4	0.3093497042671345
1101	5	0.33807107191617547
1101	6	0.33858187959469177
1101	7	0.36563782552372714
1101	8	0.4216945247786015
1101	9	0.5165583234929656
1101	10-11	0.4764981855517689
1101	12-13	0.4721554919252
1101	14-15	0.6431586514860612
1101	16-17	0.619931454764675
1101	18-19	0.6015770644595477
1101	20-21	0.6471965927717989
1101	22-23	0.7041921609599839
1101	24-25	0.7761312851697184
1101	26-27	0.7924314562179902
1101	28-29	0.7891481379383691
1101	30-31	0.7774673335816402
1101	32-33	0.6871722145029366
1101	34-35	0.7141016348500884
1101	36-37	0.7507902541682085
1101	38-39	0.7538496850590661
1101	40-41	0.8273179744228472
1101	42-43	0.8513389949844239
1101	44-45	0.8204005127113234
1101	46-47	0.8146135100960308
1101	48-49	0.7984533819353246
1101	50-51	0.7558291356100995
1101	52-53	0.7310005422532058
1101	54-55	0.8300343538147743
1101	56-57	0.7984343126791345
1101	58-59	0.7787619384033277
1101	60-61	0.7559244897117168
1101	62-63	0.7155767303247913
1101	64-65	0.6989519558569626
1101	66-67	0.6949863076195086
1101	68-69	0.7352740746769868
1101	70-71	0.7161315007821898
1101	72-73	0.7105738555452064
1101	74-75	0.7370832818349484
1101	76-77	0.6961907846701081
1101	78-79	0.6073873336734152
1101	80-81	0.5337305858821324
1101	82-83	0.5695625588225752
1101	84-85	0.512727381743904
1101	86-87	0.5461117543892975
1101	88-89	0.5626025206795191
1101	90-91	0.5696901198570572
1101	92-93	0.6215973829215322
1101	94-95	0.6755560332250568
1101	96-97	0.9622188837236934
1101	98-99	0.7572004265143448
1101	100-101	0.7215429416737287
1102	1	0.4035157573420669
1102	2	0.3842858446810098
1102	3	0.36020258935609206
1102	4	0.2651978928458263
1102	5	0.2909095605735317
1102	6	0.2689306910902616
1102	7	0.2605069630404131
1102	8	0.27971217275045035
1102	9	0.42899660452560084
1102	10-11	0.3702767417882953
1102	12-13	0.3744859017835651
1102	14-15	0.5026900511684858
1102	16-17	0.5252972067983066
1102	18-19	0.5599116674076541
1102	20-21	0.5776161747917925
1102	22-23	0.5824873663003629
1102	24-25	0.6445950923142547
1102	26-27	0.6579357150723624
1102	28-29	0.6776506549551868
1102	30-31	0.7199717116931197
1102	32-33	0.7328760280134006
1102	34-35	0.7399921796051174
1102	36-37	0.7448714868343771
1102	38-39	0.7779386669511794
1102	40-41	0.7769430818751246
1102	42-43	0.7956797500791737
1102	44-45	0.803086119968853
1102	46-47	0.8344265705526723
1102	48-49	0.8223477079290262
1102	50-51	0.8200001023501073
1102	52-53	0.7518534907819543
1102	54-55	0.7829898203891972
1102	56-57	0.7131506017744016
1102	58-59	0.8005923149298582
1102	60-61	0.829089557986137
1102	62-63	0.7760312245402687
1102	64-65	0.7582666914619409
1102	66-67	0.7853558903486615
1102	68-69	0.8431542823985652
1102	70-71	0.7885392981055261
1102	72-73	0.7277281417685657
1102	74-75	0.6399156286342702
1102	76-77	0.6358202308385188
1102	78-79	0.6668586992233827
1102	80-81	0.6885288735327109
1102	82-83	0.664351265050982
1102	84-85	0.6764365132275039
1102	86-87	0.6398433724403496
1102	88-89	0.5895443924703088
1102	90-91	0.5767304445180663
1102	92-93	0.6340215286633644
1102	94-95	0.6700156171396863
1102	96-97	1.0893660193315355
1102	98-99	0.8596096609381298
1102	100-101	0.8508294255380697
1103	1	0.31806057910696595
1103	2	0.2765768936212254
1103	3	0.24910762921345508
1103	4	0.20987479336268677
1103	5	0.20804788286232423
1103	6	0.2683742468361885
1103	7	0.2813031513640709
1103	8	0.05080900597679516
1103	9	0.16690264418131306
1103	10-11	0.3550038777020106
1103	12-13	0.36937077509268335
1103	14-15	0.46906375193273675
1103	16-17	0.46144240520997215
1103	18-19	0.3954462785791222
1103	20-21	0.4030386003068216
1103	22-23	0.4021862870922561
1103	24-25	0.25985698256054235
1103	26-27	0.2769199902916526
1103	28-29	0.46497053830162827
1103	30-31	0.3021800788648292
1103	32-33	0.49475735604440985
1103	34-35	0.5124992998871036
1103	36-37	0.5574936141505944
1103	38-39	0.5922171877218148
1103	40-41	0.46728990054162267
1103	42-43	0.5599884466700402
1103	44-45	0.685776364565541
1103	46-47	0.5317950842473564
1103	48-49	0.5463593894744605
1103	50-51	0.5359769045703899
1103	52-53	0.606726323696904
1103	54-55	0.7113543512223899
1103	56-57	0.6300677081443311
1103	58-59	0.3931679003160795
1103	60-61	0.39629633462825353
1103	62-63	0.26016802841493103
1103	64-65	0.5065067334794762
1103	66-67	0.49978566334048224
1103	68-69	0.4556385363241944
1103	70-71	0.4575321614099508
1103	72-73	0.6085208509058972
1103	74-75	0.5173057425013852
1103	76-77	0.38961523983406465
1103	78-79	0.49877767174689325
1103	80-81	0.5249272852787215
1103	82-83	0.5775145321118131
1103	84-85	0.5835744593923131
1103	86-87	0.40091948712212755
1103	88-89	0.4098505015790508
1103	90-91	0.5376588192082536
1103	92-93	0.4804991196978996
1103	94-95	0.5325409630184126
1103	96-97	0.6934577349160591
1103	98-99	0.4508394568631253
1103	100-101	0.6956165298009758
1104	1	0.34759608344431925
1104	2	0.33074681286148433
1104	3	0.2956719811725037
1104	4	0.22373767465177252
1104	5	0.24320546866380965
1104	6	0.2712083956126463
1104	7	0.2569337064793018
1104	8	0.3239901099262994
1104	9	0.32903316973909114
1104	10-11	0.3666578837661163
1104	12-13	0.36820667399296525
1104	14-15	0.4402299234660063
1104	16-17	0.4495384734809349
1104	18-19	0.4540317680956534
1104	20-21	0.4237468098717798
1104	22-23	0.5543162084634687
1104	24-25	0.4987190745471892
1104	26-27	0.5877119310496042
1104	28-29	0.5750506648039533
1104	30-31	0.6099102294050169
1104	32-33	0.6494733624647466
1104	34-35	0.6216952844830175
1104	36-37	0.6815978636855746
1104	38-39	0.6730803539194312
1104	40-41	0.6276515045312507
1104	42-43	0.714688940365555
1104	44-45	0.6661278810879239
1104	46-47	0.5296352071102959
1104	48-49	0.671248694011588
1104	50-51	0.5267909462069298
1104	52-53	0.64469561108357
1104	54-55	0.7173439016641154
1104	56-57	0.7273024554222189
1104	58-59	0.7103972882420351
1104	60-61	0.6533581673664628
1104	62-63	0.48370837442653425
1104	64-65	0.672564543554742
1104	66-67	0.7074060026777502
1104	68-69	0.7523954031359708
1104	70-71	0.708195242175556
1104	72-73	0.7966135004489558
1104	74-75	0.7587585583410714
1104	76-77	0.6536664693932437
1104	78-79	0.678730657655521
1104	80-81	0.814348211596247
1104	82-83	0.8664344868572087
1104	84-85	0.8697982912707936
1104	86-87	0.8086081186351457
1104	88-89	0.8068091767631458
1104	90-91	0.8450479040983332
1104	92-93	0.8265074891318172
1104	94-95	0.860064766575416
1104	96-97	1.2170097109158888
1104	98-99	0.9663321841450987
1104	100-101	1.0251036586638804
1105	1	0.15166757949912935
1105	2	0.13982137177620046
1105	3	0.1253865031585022
1105	4	0.0585108766469844
1105	5	0.10216566928007609
1105	6	0.04618688060938325
1105	7	0.07719602767809874
1105	8	0.1670333292843651
1105	9	0.17145834605047838
1105	10-11	0.10127555062231863
1105	12-13	0.14140913723605308
1105	14-15	0.16115008385347096
1105	16-17	0.17510387779514502
1105	18-19	0.07105022558936724
1105	20-21	0.13504488812363746
1105	22-23	0.21593909870063754
1105	24-25	0.20501254296554805
1105	26-27	0.14347316197196136
1105	28-29	0.2004517682060225
1105	30-31	0.12265347733085719
1105	32-33	0.16205476813185982
1105	34-35	0.12264213454967887
1105	36-37	0.1217172379227307
1105	38-39	0.1792726404115328
1105	40-41	0.24187815380980027
1105	42-43	0.12229734298811223
1105	44-45	0.232095686586959
1105	46-47	0.24018354387178675
1105	48-49	0.23264355073428789
1105	50-51	0.16731738067981894
1105	52-53	0.24203894283566996
1105	54-55	0.2928966065075329
1105	56-57	0.2859826826393288
1105	58-59	0.26839218645121576
1105	60-61	0.262117036020733
1105	62-63	0.2686559316768964
1105	64-65	0.25377722078458476
1105	66-67	0.3082528256290189
1105	68-69	0.26089782741602363
1105	70-71	0.2623324390363564
1105	72-73	0.315333039910783
1105	74-75	0.3724741754858343
1105	76-77	0.3390134855240703
1105	78-79	0.2649347929800001
1105	80-81	0.23680607531183995
1105	82-83	0.2756820545688612
1105	84-85	0.33480141561551946
1105	86-87	0.22324220159082486
1105	88-89	0.22503491486440197
1105	90-91	0.30836986597694604
1105	92-93	0.30213415409293987
1105	94-95	0.3663404406097932
1105	96-97	0.7211529803870285
1105	98-99	0.5242919884700861
1105	100-101	0.5460488287861942
1106	1	-0.06028251191244749
1106	2	-0.10494238414811008
1106	3	-0.05645210191017469
1106	4	-0.063479165223157
1106	5	-0.0379319909355047
1106	6	-0.025269385188700255
1106	7	-0.05537916711507762
1106	8	-0.0443426861019347
1106	9	0.02731313265140045
1106	10-11	-0.04649448516255461
1106	12-13	-0.06503885265299658
1106	14-15	-0.15524296353414968
1106	16-17	-0.10931098017618979
1106	18-19	-0.1050178894301439
1106	20-21	-0.15265882693885402
1106	22-23	-0.10466402147628173
1106	24-25	-0.003327659868581634
1106	26-27	-0.03719426084513344
1106	28-29	-0.060474942669820564
1106	30-31	-0.1795682916110195
1106	32-33	-0.27912707847553264
1106	34-35	-0.33429860101090014
1106	36-37	-0.21206421175009638
1106	38-39	-0.2606022239656909
1106	40-41	-0.25153423192004
1106	42-43	-0.20543610243960586
1106	44-45	-0.18143695164113183
1106	46-47	-0.23845388775416865
1106	48-49	-0.23494814691794375
1106	50-51	-0.16274173768763944
1106	52-53	-0.0950027924782404
1106	54-55	-0.16111508453474954
1106	56-57	-0.20296924481796452
1106	58-59	-0.12884479342708488
1106	60-61	-0.19494811403458812
1106	62-63	-0.13282855559533147
1106	64-65	-0.08328218067878623
1106	66-67	-0.0951488955262505
1106	68-69	-0.08024891462400063
1106	70-71	-0.12373353373146756
1106	72-73	-0.04763021868106421
1106	74-75	-0.06455558893630808
1106	76-77	-0.10347803299596592
1106	78-79	-0.026996916973146767
1106	80-81	-0.10233680625614738
1106	82-83	-0.10626270884317535
1106	84-85	-0.10469220732783313
1106	86-87	-0.13732551903963852
1106	88-89	-0.17488466177034923
1106	90-91	-0.10525808042198648
1106	92-93	-0.0599646663678719
1106	94-95	-0.02128229499367862
1106	96-97	0.2691012562552828
1106	98-99	0.03585221527548654
1106	100-101	0.017574010396305795
1107	1	-0.19037582541880838
1107	2	-0.2588210327775329
1107	3	-0.208791056601072
1107	4	-0.17034019212885454
1107	5	-0.169204495205868
1107	6	-0.12992871187499588
1107	7	-0.14932482733152597
1107	8	-0.13516833662186656
1107	9	-0.2225188915529941
1107	10-11	-0.25156331576695123
1107	12-13	-0.22558413862640947
1107	14-15	-0.2769101235351741
1107	16-17	-0.267757139717979
1107	18-19	-0.3092921817234142
1107	20-21	-0.29495628887495684
1107	22-23	-0.24579884305497046
1107	24-25	-0.2105539471300446
1107	26-27	-0.20642510715811824
1107	28-29	-0.20571373699537077
1107	30-31	-0.2919657334961627
1107	32-33	-0.320419287742439
1107	34-35	-0.31758854923212
1107	36-37	-0.3177886472946838
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2308	18-19	-0.9509864026631618
2308	20-21	-0.9996986936463443
2308	22-23	-1.1153890463108738
2308	24-25	-1.2797491205625136
2308	26-27	-1.2970199669775155
2308	28-29	-1.316320529961473
2308	30-31	-1.1947420399936206
2308	32-33	-1.2059854704353867
2308	34-35	-1.2911940939474462
2308	36-37	-1.363856441937827
2308	38-39	-1.3598529690438284
2308	40-41	-1.3552144421652272
2308	42-43	-1.2637267601612479
2308	44-45	-1.225441471439062
2308	46-47	-1.1962764150239167
2308	48-49	-1.3307740324597006
2308	50-51	-1.3471836549301202
2308	52-53	-1.2908116200460285
2308	54-55	-1.2432572795638563
2308	56-57	-1.167702579191399
2308	58-59	-1.1265437963859952
2308	60-61	-1.0871623233948782
2308	62-63	-1.1172870330161189
2308	64-65	-1.110384495152232
2308	66-67	-1.0719697374989394
2308	68-69	-1.1866783500734712
2308	70-71	-1.1171114379663436
2308	72-73	-1.1934474976138105
2308	74-75	-1.3284299861246325
2308	76-77	-1.2479654436076544
2308	78-79	-1.6471969400377446
2308	80-81	-1.3126814204333783
2308	82-83	-1.1837517269391356
2308	84-85	-1.1737929737764894
2308	86-87	-1.1893533157547083
2308	88-89	-1.2717591377455406
2308	90-91	-1.3357163567114156
2308	92-93	-1.238555869206003
2308	94-95	-1.3460724042106555
2308	96-97	-1.1969680070456086
2308	98-99	-1.5004659062043153
2308	100-101	-1.5258340830249502
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	64.0
3	114.0
4	291.0
5	814.0
6	1568.0
7	2805.0
8	5065.0
9	7372.0
10	9954.0
11	12512.0
12	14502.0
13	16593.0
14	18443.0
15	20570.0
16	23844.0
17	27725.0
18	33339.0
19	38453.0
20	42305.0
21	41413.0
22	44071.0
23	51494.0
24	62641.0
25	78302.0
26	98193.0
27	124869.0
28	156823.0
29	196413.0
30	242335.0
31	297046.0
32	365668.0
33	460315.0
34	613727.0
35	963071.0
36	1245613.0
37	1978077.0
38	2103886.0
39	530936.0
40	1600.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	17.421952144617535	29.70779925271086	28.464357968998243	24.405890633673362
2	23.000318393540457	29.596602835575414	25.944048930703183	21.459029840180943
3	22.722547099353203	27.963467079434356	26.44921590181209	22.864769919400356
4	22.53153926006005	27.519126319029148	26.94452378332388	23.00481063758692
5	23.23333834370426	27.884466014432483	27.525400617413275	21.356795024449973
6	23.452439416536645	27.936087876702963	25.925069058896227	22.68640364786416
7	23.615323574579882	27.897669807162632	26.926375232990086	21.560631385267396
8	22.79783460200638	28.362567535280547	26.469399863935184	22.370197998777893
9	22.204185935522457	28.03580004893358	27.293872327657642	22.46614168788632
10-11	22.127650277977285	28.484184662048843	26.607830440198992	22.780334619774877
12-13	22.154253985723702	28.61842138380356	26.312647578846143	22.914677051626597
14-15	22.467050162763346	28.741170941683663	26.053184662652907	22.738594232900084
16-17	22.70841651711205	28.32876565037986	26.13638857662462	22.826429255883472
18-19	22.562531549430144	28.572221037597963	26.147986484410378	22.71726092856152
20-21	22.709574294364966	28.40518901670079	26.30963232417441	22.575604364759837
22-23	22.555317260183223	28.284557729969368	26.435887903910448	22.72423710593696
24-25	22.48041442130067	28.264518176439957	26.38794527918385	22.86712212307552
26-27	22.764355707131628	28.04784271663361	26.684382984380978	22.503418591853787
28-29	22.75941408819605	28.1493856833896	26.55773392184661	22.533466306567735
30-31	22.63921667408651	28.169445432749956	26.532277923724827	22.659059969438708
32-33	22.660887243972663	28.225325803552785	26.461230670908765	22.65255628156579
34-35	22.67482084152083	28.23116502795881	26.45436454841754	22.639649582102816
36-37	22.681712133082772	28.224515359475745	26.43606160019314	22.657710907248347
38-39	22.68351423854601	28.223120992958094	26.42204242780453	22.67132234069136
40-41	22.674825875334975	28.285983263977442	26.40435863871974	22.634832221967844
42-43	22.69052634164738	28.263612993925396	26.364843197696203	22.681017466731017
44-45	22.68209470295765	28.28151827083249	26.37762908561974	22.65875794059012
46-47	22.669698701024128	28.332584133919603	26.342686999921977	22.655030165134292
48-49	22.692464360092483	28.30876127297508	26.344496520929695	22.65427784600274
50-51	22.760808454713693	28.28833405518228	26.2952507171675	22.655606772936522
52-53	22.694331905139585	28.394653243699224	26.22635793680469	22.6846569143565
54-55	22.69984896544045	28.418634334277073	26.14652164449473	22.73499505578775
56-57	22.746069446902624	28.485181357249186	26.109397265189184	22.659351930659007
58-59	22.74411632701509	28.56533981366431	26.054327338463395	22.636216520857204
60-61	22.721431440344663	28.577557603099745	26.04058434359103	22.660426612964564
62-63	22.7126103507944	28.602835299669653	26.035878559068742	22.648675790467202
64-65	22.684934565762294	28.63085292088422	26.10635755467468	22.57785495867881
66-67	22.648856061203638	28.64745152390686	26.105486680831508	22.598205734058
68-69	22.65742193839479	28.69876216122832	26.09728418866148	22.54653171171541
70-71	22.660245792442552	28.722270628009	26.029473125509327	22.58801045403912
72-73	22.62346053907453	28.731751342103824	26.017240119988088	22.627547998833563
74-75	22.701349476185857	28.759857349632036	26.00591554238843	22.53287763179368
76-77	22.636625452305115	28.871866347209245	25.983207014999437	22.5083011854862
78-79	22.676826604447093	28.909284917143154	25.895587404707143	22.518301073702613
80-81	22.638824889490085	28.92115017901766	25.919993105683293	22.520031825808967
82-83	22.68154832844328	28.996381353993282	25.83162536027262	22.490444957290816
84-85	22.640361034635298	29.03449071287707	25.84657578576348	22.47857246672415
86-87	22.64677242431307	29.095871202374106	25.775410092343158	22.481946280969662
88-89	22.63164707307912	29.153006197136982	25.774853821803028	22.44049290798087
90-91	22.592740745333757	29.142448524554133	25.769701240895433	22.49510948921668
92-93	22.60742645572836	29.219102689992255	25.754205379782874	22.419265474496513
94-95	22.57197932167586	29.26017234490085	25.72633437482882	22.44151395859447
96-97	22.555778655287916	29.298221220495424	25.71858038190967	22.427419742306988
98-99	22.53598740848746	29.354724275536665	25.67504475379168	22.434243562184193
100-101	22.59748312673373	29.396148880664867	25.606437707059243	22.39993028554216
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	245464.0
1	134255.5
2	17001.5
3	10049.0
4	8471.0
5	7517.0
6	7310.0
7	7268.5
8	6770.5
9	6242.5
10	6540.5
11	8421.0
12	19163.0
13	18651.5
14	8436.5
15	8341.0
16	9032.0
17	10264.5
18	12225.5
19	13611.0
20	15705.5
21	19582.5
22	24344.5
23	30300.5
24	37364.5
25	44843.5
26	53779.0
27	63545.5
28	72009.5
29	80935.0
30	93269.5
31	108555.0
32	122613.5
33	135835.5
34	150750.0
35	165276.0
36	181405.5
37	199655.5
38	216565.0
39	227759.0
40	242588.0
41	262129.5
42	277685.5
43	293770.0
44	307166.5
45	322234.0
46	336012.0
47	346902.5
48	362909.5
49	375623.5
50	378444.0
51	379489.5
52	383723.0
53	380827.0
54	364236.5
55	340820.5
56	310393.5
57	279305.5
58	259464.0
59	236621.5
60	204072.0
61	169302.0
62	135068.0
63	104641.5
64	81285.0
65	62217.5
66	48961.5
67	41924.5
68	33449.5
69	25718.0
70	20294.5
71	17042.5
72	14801.5
73	11772.5
74	9273.0
75	7144.0
76	5248.0
77	3668.0
78	2556.5
79	1792.0
80	1311.0
81	1025.5
82	851.5
83	730.0
84	590.0
85	442.5
86	318.5
87	220.0
88	127.0
89	83.5
90	61.5
91	37.0
92	23.0
93	14.0
94	6.0
95	4.0
96	3.5
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.012030815802068816
2	0.017316320652350097
3	0.005275437221995029
4	4.7317852945375264E-4
5	9.564246871937553E-4
6	0.0
7	0.0
8	0.008003764487568794
9	0.010027357773105056
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	8.557484043312547E-5
24-25	0.005215031452277529
26-27	0.007445011117681917
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	1.0067628286250056E-5
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	5.5371955574375306E-5
62-63	1.3591298186437574E-4
64-65	2.9196122030125167E-4
66-67	1.7618349500937597E-4
68-69	3.020288485875017E-4
70-71	1.4598061015062583E-4
72-73	6.543958386062537E-5
74-75	9.060865457625051E-5
76-77	0.12337878464799444
78-79	0.05439539563060905
80-81	7.047339800375039E-5
82-83	0.0024514674877018886
84-85	0.010435096718698184
86-87	0.11540522304528439
88-89	0.13172484849729574
90-91	0.1448077314552777
92-93	0.14287474682431767
94-95	0.1111013119529125
96-97	1.2013449143275035
98-99	0.07460112560111291
100-101	0.06665776688326162
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	9932826.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	67.91827153706788
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.94243811122092	61.08734934343827
2	5.569388672803443	7.565265043498688
3	1.5350366494938947	3.1277110793903176
4	0.7846162291298836	2.1315911240973486
5	0.4661936742908379	1.5831534279674258
6	0.32624531647890354	1.3294810795386491
7	0.22482353857869375	1.0688738298778528
8	0.1637581315420529	0.8897735395580826
9	0.13255239554925438	0.8102456634422778
>10	0.8368107949874047	10.565638473794474
>50	0.016309251001740785	0.673522507428969
>100	0.00132134900113861	0.15136254886255676
>500	1.264714818276261E-4	0.05274090796284069
>1k	1.264714800016372E-4	0.14585351896117385
>5k	4.215716000054573E-5	0.21800720325292067
>10k+	2.1078580000272865E-4	8.59943070892815
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	230747	2.3230750241673417	No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	28788	0.2898268831045666	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	19122	0.19251318808967358	No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	11009	0.11083451980332687	No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	10673	0.10745179669914685	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.0067628286250056E-5
2	0.0	0.0	0.0	0.0	1.0067628286250056E-5
3	1.0067628286250056E-5	0.0	0.0	0.0	1.0067628286250056E-5
4	2.0135256572500113E-5	0.0	0.0	0.0	1.0067628286250056E-5
5	3.020288485875017E-5	0.0	0.0	0.0	1.0067628286250056E-5
6	5.033814143125028E-5	1.0067628286250056E-5	0.0	0.0	2.0135256572500113E-5
7	7.047339800375039E-5	1.0067628286250056E-5	0.0	0.0	2.0135256572500113E-5
8	7.047339800375039E-5	1.0067628286250056E-5	0.0	0.0	3.020288485875017E-5
9	8.054102629000045E-5	1.0067628286250056E-5	0.0	0.0	3.020288485875017E-5
10-11	1.0067628286250055E-4	1.0067628286250056E-5	0.0	0.0	4.0270513145000225E-5
12-13	1.3591298186437574E-4	1.0067628286250056E-5	0.0	0.0	5.5371955574375306E-5
14-15	1.7114968086625096E-4	1.0067628286250056E-5	0.0	0.0	6.040576971750034E-5
16-17	2.114201940112512E-4	1.0067628286250056E-5	0.0	5.033814143125028E-6	7.047339800375039E-5
18-19	2.315554505837513E-4	1.0067628286250056E-5	0.0	1.0067628286250056E-5	7.047339800375039E-5
20-21	2.617583354425015E-4	1.0067628286250056E-5	0.0	1.0067628286250056E-5	7.047339800375039E-5
22-23	2.919612203012516E-4	1.0067628286250056E-5	0.0	1.0067628286250056E-5	7.047339800375039E-5
24-25	3.1209647687375174E-4	2.0135256572500113E-5	0.0	1.0067628286250056E-5	8.054102629000045E-5
26-27	3.372655475893769E-4	2.0135256572500113E-5	0.0	1.0067628286250056E-5	1.1074391114875061E-4
28-29	3.7250224659125206E-4	2.0135256572500113E-5	0.0	1.0067628286250056E-5	1.1074391114875061E-4
30-31	4.278742021656274E-4	2.0135256572500113E-5	0.0	1.5101442429375084E-5	1.1577772529187564E-4
32-33	4.4297564459500245E-4	2.0135256572500113E-5	0.0	2.0135256572500113E-5	1.2081153943500068E-4
34-35	4.681447153106276E-4	2.0135256572500113E-5	0.0	2.0135256572500113E-5	1.2081153943500068E-4
36-37	5.18482856741878E-4	2.0135256572500113E-5	0.0	2.0135256572500113E-5	1.4094679600750077E-4
38-39	5.839224406025032E-4	2.0135256572500113E-5	0.0	2.0135256572500113E-5	1.4094679600750077E-4
40-41	6.241929537475035E-4	2.0135256572500113E-5	0.0	2.0135256572500113E-5	1.5101442429375084E-4
42-43	6.896325376081289E-4	3.020288485875017E-5	0.0	2.0135256572500113E-5	1.5604823843687587E-4
44-45	7.19835422466879E-4	3.020288485875017E-5	0.0	2.0135256572500113E-5	1.610820525800009E-4
46-47	7.601059356118793E-4	3.020288485875017E-5	0.0	2.0135256572500113E-5	1.7114968086625096E-4
48-49	8.003764487568795E-4	4.0270513145000225E-5	0.0	2.0135256572500113E-5	1.8625112329562603E-4
50-51	8.305793336156296E-4	5.033814143125028E-5	0.0	3.020288485875017E-5	1.963187515818761E-4
52-53	9.010527316193799E-4	5.033814143125028E-5	0.0	3.020288485875017E-5	2.0135256572500113E-4
54-55	9.564246871937553E-4	5.033814143125028E-5	0.0	3.020288485875017E-5	2.0638637986812616E-4
56-57	0.0010218642710543807	5.5371955574375306E-5	0.0	3.020288485875017E-5	2.114201940112512E-4
58-59	0.0011376419963462564	6.040576971750034E-5	0.0	3.5236699001875194E-5	2.164540081543762E-4
60-61	0.001228250650922507	6.040576971750034E-5	0.0	4.0270513145000225E-5	2.2652163644062626E-4
62-63	0.0013540960045006325	6.040576971750034E-5	0.0	4.0270513145000225E-5	2.3658926472687632E-4
64-65	0.0015101442429375084	6.040576971750034E-5	0.0	4.0270513145000225E-5	2.516907071562514E-4
66-67	0.0017014291803762595	6.040576971750034E-5	0.0	4.0270513145000225E-5	2.5672452129937645E-4
68-69	0.0018323083480975102	6.040576971750034E-5	0.0	4.0270513145000225E-5	2.667921495856265E-4
70-71	0.0020084918431068862	6.040576971750034E-5	0.0	4.0270513145000225E-5	2.7685977787187657E-4
72-73	0.002179641523973137	6.040576971750034E-5	0.0	5.033814143125028E-5	2.919612203012516E-4
74-75	0.0024363660452725137	6.040576971750034E-5	0.0	5.033814143125028E-5	2.919612203012516E-4
76-77	0.0027685977787187655	6.040576971750034E-5	0.0	5.5371955574375306E-5	2.9699503444437664E-4
78-79	0.003080694255592517	6.040576971750034E-5	0.0	6.040576971750034E-5	3.1209647687375174E-4
80-81	0.003347486405178144	6.040576971750034E-5	0.0	6.040576971750034E-5	3.52366990018752E-4
82-83	0.003775360607343771	6.040576971750034E-5	0.0	6.040576971750034E-5	3.976713173068772E-4
84-85	0.004298877278228774	6.543958386062536E-5	0.0	6.040576971750034E-5	4.228403880225024E-4
86-87	0.004968374559264403	7.047339800375039E-5	0.0	6.040576971750034E-5	4.4297564459500245E-4
88-89	0.0058291567777387825	7.047339800375039E-5	0.0	6.040576971750034E-5	4.4297564459500245E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGCAGT	12150	0.0	31.500282	1
AGTGGTA	14775	0.0	25.386368	5
GGTATCA	15415	0.0	24.36277	8
GTATCAA	15705	0.0	23.850115	9
TGGTATC	16555	0.0	22.62783	7
AGCAGTG	17300	0.0	22.149887	2
GTGGTAT	17350	0.0	21.618324	6
ATCAACG	14350	0.0	21.160337	10-11
CAACGCA	14560	0.0	20.871424	12-13
GCAGTGG	18940	0.0	20.00538	3
CGTCACG	695	0.0	19.787603	3
CAGTGGT	20335	0.0	18.6786	4
ACGCAGA	16575	0.0	18.348412	14-15
CGCGTAG	375	0.0	18.331253	54-55
AGAGTAC	16885	0.0	17.52121	18-19
AGTACTT	13140	0.0	16.436138	20-21
TCAACGC	14445	0.0	16.147413	10-11
AACGCAG	15075	0.0	15.535492	12-13
GCAGAGT	20130	0.0	15.237616	16-17
ACTCGCG	455	0.0	15.108175	50-51
>>END_MODULE