FastQCFastQC Report
Sat 18 Jun 2016
SRR578607_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR578607_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13511971
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT219380816.236032478163253No Hit
AAGCAGTGGTATCAACGCAGAGTATTTTTTTTTTTTTTTTTTTTTTTTTT509230.3768732185704069No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA341500.2527388491286726No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAG256960.1901721073853696No Hit
AAGCAGTGGTATCAACGCAGAGTACCTTTTTTTTTTTTTTTTTTTTTTTT145990.10804493289691046No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT4634750.092.534061
AGTGGTA4636150.091.802175
AGCAGTG4670400.091.718012
GCAGTGG4676700.091.368553
GGTATCA4664150.091.317718
TGGTATC4666050.091.2879647
GTATCAA4666100.091.2554559
GTGGTAT4675700.091.2137156
CAGTGGT4688300.090.797694
ATCAACG4673300.046.06950410-11
CAACGCA4682850.046.03091412-13
TCAACGC4677550.046.00892612-13
AACGCAG4697950.045.90190514-15
ACGCAGA4707600.045.8794614-15
CGCAGAG4718900.045.7612116-17
TATCAAC4690500.045.7395110-11
AGAGTAC4598650.045.6854218-19
CAGAGTA4683400.045.5716618-19
GCAGAGT4726550.045.45199216-17
GAGTACT2685750.045.4342720-21
Produced by FastQC (version 0.11.5)