##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR578607_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 13511971 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.566703184901744 31.0 31.0 33.0 27.0 34.0 2 30.86383437323837 31.0 31.0 34.0 27.0 34.0 3 30.966421035095472 31.0 31.0 34.0 27.0 34.0 4 34.35460600085657 37.0 35.0 37.0 30.0 37.0 5 34.44607955419679 37.0 35.0 37.0 30.0 37.0 6 34.44391599123473 37.0 35.0 37.0 30.0 37.0 7 34.39112694957679 37.0 35.0 37.0 30.0 37.0 8 34.37105060394224 37.0 35.0 37.0 30.0 37.0 9 35.76098350122273 39.0 35.0 39.0 30.0 39.0 10-11 35.689440311853836 39.0 35.0 39.0 30.0 39.0 12-13 35.78625879229611 39.0 35.0 39.0 30.0 39.0 14-15 36.86006416088371 40.0 36.0 41.0 30.0 41.0 16-17 36.80605198160949 40.0 36.0 41.0 30.0 41.0 18-19 36.760843958294465 40.0 36.0 41.0 30.0 41.0 20-21 36.59839741367118 39.5 36.0 41.0 30.0 41.0 22-23 36.261638290964356 39.0 35.5 40.5 28.5 41.0 24-25 36.19105258588847 39.0 36.0 40.0 29.0 41.0 26-27 36.109157279866864 39.0 35.5 40.5 27.5 41.0 28-29 35.8699533176914 39.0 35.0 41.0 27.0 41.0 30-31 35.60186156409009 39.0 34.5 41.0 26.0 41.0 32-33 35.17664025477852 38.0 34.0 40.0 25.0 41.0 34-35 34.9645590935623 38.0 34.0 40.0 25.0 41.0 36-37 34.58708722065789 38.0 33.0 39.5 24.0 41.0 38-39 33.69058788684493 35.0 33.0 39.0 24.0 40.5 40-41 33.813432141025174 36.0 33.0 40.0 24.0 41.0 42-43 33.701057861950716 36.0 33.0 40.0 22.5 41.0 44-45 33.55132482152308 36.0 33.0 40.0 22.5 41.0 46-47 33.33958913174103 35.0 33.0 40.0 21.5 41.0 48-49 32.99399636070859 35.0 33.0 40.0 20.0 41.0 50-51 31.760410490815886 35.0 31.0 39.0 14.5 40.5 52-53 31.314388663208348 35.0 30.0 39.0 7.0 40.0 54-55 30.687168659553812 35.0 29.0 39.0 2.0 41.0 56-57 30.077873797982544 35.0 27.5 39.0 2.0 41.0 58-59 29.794696569434613 35.0 27.0 39.0 2.0 41.0 60-61 29.54257935426297 35.0 27.0 39.0 2.0 41.0 62-63 29.223165036396246 35.0 26.5 39.0 2.0 40.5 64-65 28.892721757617746 35.0 26.0 38.0 2.0 40.0 66-67 28.58007096077989 35.0 26.0 37.5 2.0 40.0 68-69 28.250877462658853 35.0 26.0 37.0 2.0 40.0 70-71 27.884315878120226 34.0 25.5 36.5 2.0 39.0 72-73 27.50026665243731 34.0 24.5 36.0 2.0 39.0 74-75 27.14030651042694 34.0 24.0 35.5 2.0 39.0 76-77 26.759727688876776 34.0 22.5 35.0 2.0 37.5 78-79 26.41351528211539 34.0 21.5 35.0 2.0 37.0 80-81 26.066385170601684 33.0 20.0 35.0 2.0 36.5 82-83 25.70353792203965 33.0 19.5 35.0 2.0 36.0 84-85 25.362342436939805 33.0 18.0 35.0 2.0 36.0 86-87 25.037235019228504 33.0 13.5 35.0 2.0 35.0 88-89 24.678855216607555 32.0 7.5 35.0 2.0 35.0 90-91 24.309138318902548 32.0 4.5 35.0 2.0 35.0 92-93 23.936104288560124 32.0 2.0 35.0 2.0 35.0 94-95 23.500308800248312 32.0 2.0 35.0 2.0 35.0 96-97 22.998376254655966 31.0 2.0 35.0 2.0 35.0 98-99 22.458360664036356 31.0 2.0 34.5 2.0 35.0 100-101 21.166271116182827 29.0 2.0 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.6070915374273973 1101 2 0.6229955456969911 1101 3 0.632053232223587 1101 4 0.6684193342488527 1101 5 0.6719818466487695 1101 6 0.5750372763749567 1101 7 0.5467475262243511 1101 8 0.6328782841295322 1101 9 0.793370536913919 1101 10-11 0.7625928427642634 1101 12-13 0.8214816514846959 1101 14-15 0.8993082175840001 1101 16-17 0.821482384657493 1101 18-19 0.88841715191991 1101 20-21 0.8668411007126764 1101 22-23 0.8794482286037564 1101 24-25 0.953809894288149 1101 26-27 1.0534259307664584 1101 28-29 1.1157864551148649 1101 30-31 1.1833554387016036 1101 32-33 1.1066152720776117 1101 34-35 1.2126509160866803 1101 36-37 1.2295686817920526 1101 38-39 1.091268715377666 1101 40-41 1.1546682845090928 1101 42-43 1.441490821934444 1101 44-45 1.4272801371851784 1101 46-47 1.3562796092632041 1101 48-49 1.3840677453455186 1101 50-51 1.322093865532075 1101 52-53 1.264026643131512 1101 54-55 1.103433725450646 1101 56-57 1.086319015998857 1101 58-59 1.0477254842661026 1101 60-61 1.1790791066791897 1101 62-63 1.1950448149421398 1101 64-65 1.0528224319916113 1101 66-67 1.076203571480761 1101 68-69 1.073596753225221 1101 70-71 1.126597018566347 1101 72-73 1.1941406551670184 1101 74-75 1.160435814357072 1101 76-77 1.2150136949193282 1101 78-79 1.1881862500662876 1101 80-81 1.2754147708940735 1101 82-83 1.2536553657047094 1101 84-85 1.1998116680708044 1101 86-87 1.1829815040015816 1101 88-89 1.2745646838911533 1101 90-91 1.2411315724423027 1101 92-93 1.4592257144992828 1101 94-95 1.5479913648329209 1101 96-97 1.6427547645258507 1101 98-99 1.753317216724927 1101 100-101 2.0656864987456807 1102 1 0.538112480132046 1102 2 0.5257750320261607 1102 3 0.49621074373594354 1102 4 0.6443382808235114 1102 5 0.5360473045590339 1102 6 0.4528069225622602 1102 7 0.42914030040243745 1102 8 0.5524000970391967 1102 9 0.637294235091673 1102 10-11 0.7536539735688166 1102 12-13 0.659788608061838 1102 14-15 0.6950738462983068 1102 16-17 0.706604526440934 1102 18-19 0.6847577374171792 1102 20-21 0.7960030039758408 1102 22-23 0.7791769103165436 1102 24-25 0.7265262961630086 1102 26-27 0.9306838022026582 1102 28-29 0.8001977036029544 1102 30-31 1.0420815062163982 1102 32-33 1.0163118905438395 1102 34-35 1.0394385236804098 1102 36-37 1.005458742722766 1102 38-39 0.9926540188822841 1102 40-41 1.059527819848448 1102 42-43 1.1820482732865045 1102 44-45 1.0698197979032074 1102 46-47 1.1752658206797477 1102 48-49 1.1369608836859726 1102 50-51 1.086694001936543 1102 52-53 1.0125219144743731 1102 54-55 0.9118934421012419 1102 56-57 0.9158947957285797 1102 58-59 0.7635792830675143 1102 60-61 0.9385996792284352 1102 62-63 1.0088014165394164 1102 64-65 0.8606150209764749 1102 66-67 0.9534928506818261 1102 68-69 0.9663920271752282 1102 70-71 0.8850318271471025 1102 72-73 0.956263457205484 1102 74-75 0.9720751186016585 1102 76-77 0.9674723728618879 1102 78-79 0.838236914145245 1102 80-81 0.8583450858012469 1102 82-83 0.8584698236300881 1102 84-85 0.8259282196825914 1102 86-87 0.8966320035310602 1102 88-89 0.9619273533513208 1102 90-91 1.0240499417262896 1102 92-93 1.1569887462910629 1102 94-95 1.1759328389377188 1102 96-97 1.3012150394324564 1102 98-99 1.4191796995739487 1102 100-101 1.5797478096112592 1103 1 0.3829494157786577 1103 2 0.4812228393730429 1103 3 0.4547580581750985 1103 4 0.4996069196237656 1103 5 0.48041128021064594 1103 6 0.3373086284930551 1103 7 0.28046607498559695 1103 8 0.3668032058872228 1103 9 0.4767751366596116 1103 10-11 0.5547043106717453 1103 12-13 0.4955059065003127 1103 14-15 0.6002916355240657 1103 16-17 0.5602739936500925 1103 18-19 0.6089820117802205 1103 20-21 0.6625533922848064 1103 22-23 0.5238979937511132 1103 24-25 0.588510944956603 1103 26-27 0.7220104320475969 1103 28-29 0.6776693827388911 1103 30-31 0.6994971388571756 1103 32-33 0.7749458073791189 1103 34-35 0.7645958993890289 1103 36-37 0.7098341939173451 1103 38-39 0.7717039461998851 1103 40-41 0.6885617512800835 1103 42-43 0.890151556509224 1103 44-45 0.8143037649779856 1103 46-47 0.7222880472951942 1103 48-49 0.9047438052209245 1103 50-51 0.8154147494357957 1103 52-53 0.8280993231147953 1103 54-55 0.7752935400073504 1103 56-57 0.7301958238386987 1103 58-59 0.6796119295271552 1103 60-61 0.7464472739092045 1103 62-63 0.7524844696836901 1103 64-65 0.7730375909944946 1103 66-67 0.83016369014857 1103 68-69 0.7987400214732396 1103 70-71 0.7302065032272687 1103 72-73 0.7321504354232466 1103 74-75 0.7424242025072978 1103 76-77 0.7240005645798178 1103 78-79 0.7270323175667208 1103 80-81 0.7915946912223504 1103 82-83 0.8111425544067039 1103 84-85 0.90833576583805 1103 86-87 0.9261457327105305 1103 88-89 0.9512809836028495 1103 90-91 0.9189083011893224 1103 92-93 0.977177473753386 1103 94-95 1.0629360484886483 1103 96-97 1.079411390126623 1103 98-99 1.1488037959109292 1103 100-101 1.3208716638106672 1104 1 0.3441943840289312 1104 2 0.23154079502645075 1104 3 0.3853367543232089 1104 4 0.46485022525538255 1104 5 0.33750430992147784 1104 6 0.3087897181672048 1104 7 0.29377650048211024 1104 8 0.31803624861048974 1104 9 0.4459028828899321 1104 10-11 0.48469249059554187 1104 12-13 0.4566508314705757 1104 14-15 0.4150684155705022 1104 16-17 0.567608799349884 1104 18-19 0.4839953308665699 1104 20-21 0.5357095403937961 1104 22-23 0.5132983700815501 1104 24-25 0.544158855727197 1104 26-27 0.5956038814349895 1104 28-29 0.46378452749926424 1104 30-31 0.5365144984171479 1104 32-33 0.5873394158396579 1104 34-35 0.622364260494706 1104 36-37 0.6967012827794861 1104 38-39 0.6820153619202003 1104 40-41 0.699203332137678 1104 42-43 0.7343371684536706 1104 44-45 0.8051094109747723 1104 46-47 0.7214896513991249 1104 48-49 0.7852271725221982 1104 50-51 0.6693621029523484 1104 52-53 0.6880525763447487 1104 54-55 0.7703790175235063 1104 56-57 0.7019237697622067 1104 58-59 0.6148157066448405 1104 60-61 0.5940829479351919 1104 62-63 0.7549666157170272 1104 64-65 0.8081212186589717 1104 66-67 0.7146411856142407 1104 68-69 0.7003800067731554 1104 70-71 0.7300793727547017 1104 72-73 0.6577766462480241 1104 74-75 0.746314477609598 1104 76-77 0.7585562660842768 1104 78-79 0.7643595157129184 1104 80-81 0.6485643589218633 1104 82-83 0.6512136319933468 1104 84-85 0.748169887650274 1104 86-87 0.7498810817430979 1104 88-89 0.8354044503156857 1104 90-91 0.8031431881887023 1104 92-93 0.9735813204399513 1104 94-95 1.013912337242342 1104 96-97 0.9552103755334436 1104 98-99 1.093781063494884 1104 100-101 1.3320928742267562 1105 1 0.03135371290068889 1105 2 0.09778307263625763 1105 3 0.1947277766899056 1105 4 0.21314081560821307 1105 5 0.2582302458019896 1105 6 0.2934417651072465 1105 7 0.2755827864113911 1105 8 0.3181015583337441 1105 9 0.27414628992248424 1105 10-11 0.20529414574448168 1105 12-13 0.2306654000406283 1105 14-15 0.12966450731646972 1105 16-17 0.19901624368969095 1105 18-19 0.12409228562750485 1105 20-21 0.4031995630767966 1105 22-23 0.37754254732023895 1105 24-25 0.3646410822793271 1105 26-27 0.5857205709421152 1105 28-29 0.5594752724018051 1105 30-31 0.5618506893183408 1105 32-33 0.6081362369071286 1105 34-35 0.6188268036953559 1105 36-37 0.5878331783947033 1105 38-39 0.7631679381248233 1105 40-41 0.670185181487156 1105 42-43 0.8284402903477357 1105 44-45 0.7428428828608844 1105 46-47 0.7313650483475627 1105 48-49 0.8763360329730574 1105 50-51 0.8663707006808892 1105 52-53 0.7184709988745048 1105 54-55 0.8870232102142239 1105 56-57 0.9038944014930266 1105 58-59 0.948002237118768 1105 60-61 0.8876452897719886 1105 62-63 0.8906164237727303 1105 64-65 0.9203206074528332 1105 66-67 1.0018114216772958 1105 68-69 0.9639499442468562 1105 70-71 0.7970471871271236 1105 72-73 0.9288205311672613 1105 74-75 0.7840716055568144 1105 76-77 0.7061333678029698 1105 78-79 0.82397436032673 1105 80-81 0.7185199631233203 1105 82-83 0.7206253500778992 1105 84-85 0.748053338186363 1105 86-87 0.8697011087793172 1105 88-89 0.9432426829270995 1105 90-91 0.9630734052773207 1105 92-93 1.0483578438358663 1105 94-95 1.140473719221088 1105 96-97 1.1208570240546578 1105 98-99 1.2299322509289254 1105 100-101 1.4385473449291943 1106 1 0.20766416173300328 1106 2 0.3279279316520558 1106 3 0.2990914883975506 1106 4 0.4614177801413746 1106 5 0.3658361803030914 1106 6 0.35576203075588353 1106 7 0.3156367131570761 1106 8 0.319882540344949 1106 9 0.1745382027288116 1106 10-11 0.33758813175436586 1106 12-13 0.3323868994210599 1106 14-15 0.34835430534378986 1106 16-17 0.38703088319991963 1106 18-19 0.25779581454278144 1106 20-21 0.36293340518295736 1106 22-23 0.39638743738137094 1106 24-25 0.43744423015575506 1106 26-27 0.28041404069496423 1106 28-29 0.32396088922534005 1106 30-31 0.5053677915274193 1106 32-33 0.5167262205081542 1106 34-35 0.46919423705400476 1106 36-37 0.525092325262321 1106 38-39 0.7072702488032903 1106 40-41 0.5333574172884923 1106 42-43 0.4569858023844162 1106 44-45 0.5932004615279851 1106 46-47 0.6190829043609725 1106 48-49 0.5833665812288018 1106 50-51 0.6327150299587103 1106 52-53 0.7450856412503803 1106 54-55 0.5945042879290376 1106 56-57 0.5608780038398358 1106 58-59 0.7171649815667465 1106 60-61 0.6210547741719736 1106 62-63 0.5080627534672466 1106 64-65 0.6193846049641003 1106 66-67 0.720283397223227 1106 68-69 0.7283690771642952 1106 70-71 0.6195306097422382 1106 72-73 0.6768086172470298 1106 74-75 0.6724565041646784 1106 76-77 0.6251344112133452 1106 78-79 0.6363236657793543 1106 80-81 0.6034950586172236 1106 82-83 0.5975926637346518 1106 84-85 0.5814820736983428 1106 86-87 0.755607431374834 1106 88-89 0.734161829236065 1106 90-91 0.6954709195722906 1106 92-93 0.7228169945186806 1106 94-95 0.7066666364725087 1106 96-97 0.8352523508263268 1106 98-99 0.9268313486170818 1106 100-101 1.1228297149536068 1107 1 0.09692725868439567 1107 2 0.11637191297146998 1107 3 0.2730895419010295 1107 4 0.484697148507621 1107 5 0.23657907628559371 1107 6 0.2579328216408072 1107 7 0.28631421659151357 1107 8 0.19867509859983556 1107 9 0.1694211355630273 1107 10-11 0.21086024488938193 1107 12-13 0.16961193816232623 1107 14-15 0.33265373367651563 1107 16-17 0.23609648220120505 1107 18-19 0.32437080324294953 1107 20-21 0.21792417732274316 1107 22-23 0.29379934180722245 1107 24-25 0.5109079062635047 1107 26-27 0.26363941854954476 1107 28-29 0.2590031998058109 1107 30-31 0.39300814992081 1107 32-33 0.6298326611104486 1107 34-35 0.4095945583995402 1107 36-37 0.5136086918918394 1107 38-39 0.5666217085973884 1107 40-41 0.3589092829624505 1107 42-43 0.35395348094969137 1107 44-45 0.5515338462366088 1107 46-47 0.3911017117432962 1107 48-49 0.5986822466342829 1107 50-51 0.35994052720165826 1107 52-53 0.49633629135588464 1107 54-55 0.37225784171641507 1107 56-57 0.36777697202790804 1107 58-59 0.6502377977061187 1107 60-61 0.607554698451299 1107 62-63 0.4590235423748332 1107 64-65 0.5612978518145333 1107 66-67 0.6408617094129099 1107 68-69 0.5843410496787698 1107 70-71 0.4746080409380724 1107 72-73 0.44443704335900946 1107 74-75 0.5283128393591809 1107 76-77 0.5457705160502115 1107 78-79 0.39280785798290907 1107 80-81 0.3096697408511808 1107 82-83 0.3261307722821627 1107 84-85 0.3296932915089492 1107 86-87 0.5271724271782148 1107 88-89 0.5213266335082061 1107 90-91 0.4633669732181467 1107 92-93 0.5018907267353754 1107 94-95 0.429116414370764 1107 96-97 0.5171193343206255 1107 98-99 0.517943099608491 1107 100-101 0.599976392099066 1108 1 0.23733010082398565 1108 2 0.22970042514581124 1108 3 0.23541161829075463 1108 4 0.45051930237022475 1108 5 0.27819452608259354 1108 6 0.3889703503387736 1108 7 0.40783995540663653 1108 8 0.3459455869772654 1108 9 0.3629855253576224 1108 10-11 0.38880318089006494 1108 12-13 0.18217650548753994 1108 14-15 0.41128534691970486 1108 16-17 0.30540505471940804 1108 18-19 0.31267732732285936 1108 20-21 0.33915782792463744 1108 22-23 0.2726353658063658 1108 24-25 0.5003561336480331 1108 26-27 0.4597031679346557 1108 28-29 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-2.6507229929108505 2315 42-43 -2.146024060600915 2315 44-45 -1.8345493309722336 2315 46-47 -1.5088326507476424 2315 48-49 -1.9666621091051084 2315 50-51 -1.7082788018524795 2315 52-53 -1.568109912963589 2315 54-55 -1.4825822719902106 2315 56-57 -1.6093468346780213 2315 58-59 -1.7237720710866427 2315 60-61 -1.455909492828333 2315 62-63 -1.2817982358710296 2315 64-65 -1.2083651197603409 2315 66-67 -1.2586300757287745 2315 68-69 -1.538891824929614 2315 70-71 -1.1412494454052187 2315 72-73 -1.1182642446792244 2315 74-75 -1.247157102737674 2315 76-77 -1.1661179724819384 2315 78-79 -1.393307226487913 2315 80-81 -1.4232434176155486 2315 82-83 -1.2635776358690194 2315 84-85 -1.4202805201213344 2315 86-87 -1.5350451790473727 2315 88-89 -1.5416007816969994 2315 90-91 -1.5202461223166104 2315 92-93 -1.5812190445733698 2315 94-95 -1.4661614696680871 2315 96-97 -1.5420749317114897 2315 98-99 -1.603307184225411 2315 100-101 -1.79123432654821 2316 1 -1.010012103334386 2316 2 -1.1701724835868639 2316 3 -1.5112739385879763 2316 4 -1.4312090421069925 2316 5 -0.8833215247935868 2316 6 -0.8821024036148586 2316 7 -1.1609815073987946 2316 8 -1.2454903120375036 2316 9 -1.4805231749450911 2316 10-11 -1.4863718089935958 2316 12-13 -1.3711032076748282 2316 14-15 -1.369352573606541 2316 16-17 -2.090733331399271 2316 18-19 -1.4291479032050418 2316 20-21 -1.8425265401572233 2316 22-23 -1.3866932587434135 2316 24-25 -1.3054959245421642 2316 26-27 -1.4007102582746995 2316 28-29 -1.7279945554810752 2316 30-31 -1.632337869820141 2316 32-33 -1.7210985893765596 2316 34-35 -2.15262027985532 2316 36-37 -1.8769888867566493 2316 38-39 -1.8525358973288206 2316 40-41 -2.208216506291187 2316 42-43 -2.402449686279631 2316 44-45 -2.634460997685199 2316 46-47 -2.5347235308258504 2316 48-49 -3.2114874918149425 2316 50-51 -2.741476186545711 2316 52-53 -3.386121523779668 2316 54-55 -4.574562897107523 2316 56-57 -3.287805336457886 2316 58-59 -2.771076866586995 2316 60-61 -2.50168777691713 2316 62-63 -3.0349480391192536 2316 64-65 -3.05927880938118 2316 66-67 -2.8426451105459876 2316 68-69 -2.8097742742910583 2316 70-71 -3.071113145779588 2316 72-73 -5.9733279154312875 2316 74-75 -3.8337337574828325 2316 76-77 -3.184588461781651 2316 78-79 -3.15900616811485 2316 80-81 -3.086981289118981 2316 82-83 -3.142589233765758 2316 84-85 -3.161025315109544 2316 86-87 -3.007653630876071 2316 88-89 -2.799109816354836 2316 90-91 -2.789646884226187 2316 92-93 -3.144720091871651 2316 94-95 -3.3147388635441963 2316 96-97 -3.337650481938507 2316 98-99 -3.6377645615545653 2316 100-101 -3.6711621161752035 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 137891.0 3 25701.0 4 22706.0 5 23067.0 6 26347.0 7 33493.0 8 42217.0 9 49866.0 10 58074.0 11 69161.0 12 70106.0 13 77958.0 14 87042.0 15 102376.0 16 126546.0 17 164366.0 18 228756.0 19 335878.0 20 473351.0 21 353197.0 22 203653.0 23 186612.0 24 199199.0 25 210221.0 26 227039.0 27 254550.0 28 289951.0 29 336853.0 30 394829.0 31 468674.0 32 565256.0 33 695907.0 34 902909.0 35 1281787.0 36 1843392.0 37 1672586.0 38 1117660.0 39 152699.0 40 95.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 10.443305231653653 52.728845954395275 20.279251181604018 16.548597632347057 2 15.718942433631009 53.67028664192613 16.2434992397926 14.367271684650254 3 50.058070004744415 18.048264140559116 16.60071029182409 15.292955562872383 4 15.357089624456908 16.95636794813897 17.630421934451444 50.05612049295268 5 15.515347385142356 51.4810865834326 18.416595540579493 14.586970490845546 6 49.92958680778787 17.73063514988332 16.816459982780234 15.523318059548574 7 16.147725872792236 17.336361803905074 52.012567150329545 14.503345172973145 8 49.544747294416304 17.959567758440592 17.527647670656616 14.968037276486484 9 49.11436136154011 17.72803046087043 18.094866845902573 15.062741331686885 10-11 15.052679179591227 34.87285734902339 34.699574009321054 15.374889462064328 12-13 14.897318235207402 18.18113951813485 34.516153914204075 32.405388332453676 14-15 15.139774344547199 52.039415472305265 17.344409105838295 15.476401077309237 16-17 32.29336256064486 17.893987996870745 17.452882605808508 32.35976683667588 18-19 15.378256856575238 34.91052114148341 17.440535273621716 32.27068672831964 20-21 32.416790769014206 34.92741553805203 17.55149607211118 15.104297620822582 22-23 32.288395103191256 17.895179879049124 34.60680969059707 15.209615327162549 24-25 15.18194406765003 34.61371694887745 18.162287097993268 32.042051885479246 26-27 15.435689203491282 24.5011426886658 44.65558150806472 15.4075865997782 28-29 29.16442676570617 17.654738639514175 37.92942561825246 15.251408976527195 30-31 26.016007603609864 19.44886192200888 38.47498108148217 16.06014939289909 32-33 19.22506127291293 22.03535645494886 40.38025632156404 18.35932595057417 34-35 18.572026181288493 21.04396090378011 41.1839151140336 19.200097800897794 36-37 18.76230275515817 20.867272000167635 41.266235009103255 19.104190235570943 38-39 18.983872461808136 20.849035807898932 41.10581111329055 19.06128061700238 40-41 18.90205421722476 20.853445441095555 40.8302597957741 19.414240545905585 42-43 19.316031659583253 20.96434292992531 40.880776918599864 18.838848491891575 44-45 18.726278909122115 21.122332614075415 40.74062487250208 19.41076360430038 46-47 19.229028972775343 20.958984388738152 40.55501073415393 19.25697590433257 48-49 19.051942697165533 21.173138350506353 40.62758554974447 19.147333402583648 50-51 19.232003728130774 20.887320594072367 40.35788559779961 19.52279007999725 52-53 19.655665827726818 21.851977456386745 39.0348839030127 19.457472812873743 54-55 20.207049333756306 22.649024161863977 37.43788573666932 19.706040767710398 56-57 20.521518117981614 23.462980731178508 36.22499791392457 19.79050323691531 58-59 20.641088081165517 24.344320362999774 35.18157115653421 19.833020399300494 60-61 20.569983117863966 24.595606201963662 34.75068120899788 20.083729471174493 62-63 20.433959854796292 24.701498579962763 34.60398734475048 20.26055422049047 64-65 20.231926267967644 24.293877281435815 34.881415114314336 20.592781336282204 66-67 20.255969912129316 23.76874258595626 35.717259251517866 20.258028250396553 68-69 20.095455768475837 23.689646103678307 36.01735474616331 20.197543381682546 70-71 20.095267333435075 23.62341750242265 36.35294726579117 19.9283678983511 72-73 19.732906241169783 23.387379164471586 36.461639797133685 20.418074797224943 74-75 20.28523194254193 23.553974306763912 35.870637888267744 20.290155862426417 76-77 20.649129463082517 24.08692915162856 35.047063133695964 20.21687825159296 78-79 20.409011491393347 24.148918506639006 34.8761465062414 20.565923495726242 80-81 20.279223814673735 24.840027290142796 34.395660646652296 20.485088248531177 82-83 21.25823123288158 24.834707125908455 33.59057137319964 20.31649026801033 84-85 21.002993143546316 25.38727040864585 33.21641892740612 20.393317520401713 86-87 20.763666680229186 25.622853837501726 33.110205799469696 20.50327368279939 88-89 20.5679361171266 25.212779654714822 33.08633210336803 21.132952124790553 90-91 20.57079585719945 25.339626296772366 33.07641142339531 21.01316642263287 92-93 20.837680945888465 25.480342547322742 33.1438782419221 20.53809826486669 94-95 20.70045339674392 25.33216590606937 32.53471850989468 21.43266218729204 96-97 20.644158155056815 25.058892077170874 32.96728528039906 21.32966448737325 98-99 21.027269666255428 25.46342677952389 32.35189118371558 21.157412370505103 100-101 21.546103330231116 24.972325061967876 32.17941265912442 21.302158948676592 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 40008.0 1 23823.5 2 5299.5 3 2518.5 4 1931.0 5 1687.5 6 2077.5 7 2677.0 8 2348.0 9 2399.5 10 3971.5 11 27464.5 12 480749.0 13 522368.0 14 111738.5 15 90036.0 16 92461.5 17 97207.5 18 100826.0 19 102060.5 20 101666.0 21 101615.0 22 101632.0 23 102516.5 24 105653.5 25 109995.5 26 115731.5 27 123785.0 28 130352.5 29 136355.5 30 144977.5 31 155694.5 32 166047.5 33 178267.0 34 200680.5 35 215688.5 36 224932.0 37 240401.0 38 253536.0 39 266939.0 40 284201.0 41 303257.0 42 320053.5 43 335417.0 44 351801.5 45 366332.5 46 374059.0 47 393622.5 48 410165.0 49 414219.0 50 419227.0 51 417462.5 52 420737.5 53 428464.0 54 413190.5 55 385782.0 56 368689.0 57 350697.5 58 353263.0 59 314958.0 60 239700.5 61 195459.5 62 157079.5 63 122922.5 64 96918.0 65 78889.0 66 63229.5 67 50617.5 68 39670.5 69 30538.5 70 24795.5 71 21024.5 72 17876.5 73 14493.5 74 11357.0 75 8911.5 76 7195.5 77 5745.5 78 4571.0 79 3779.5 80 3231.5 81 2875.5 82 2617.0 83 2319.5 84 1988.5 85 1806.5 86 1560.5 87 1246.5 88 960.5 89 723.0 90 565.0 91 452.0 92 351.5 93 251.5 94 174.5 95 136.0 96 94.5 97 57.0 98 37.0 99 22.0 100 9.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0989566955109658 2 0.08177933478394825 3 0.08796644101737637 4 0.19760995638608164 5 0.0749483550549361 6 0.09154844988936107 7 0.09597415506590415 8 0.08553156308579998 9 0.07833054111794645 10-11 0.07685407258496928 12-13 0.07031172580225342 14-15 0.07674305991331687 16-17 0.06716636677210157 18-19 0.059199357369846335 20-21 0.054207487567875925 22-23 0.04373529220866445 24-25 0.04608506042530731 26-27 0.04518215736253431 28-29 0.0442533513430424 30-31 0.034802472563033184 32-33 0.03838818185740629 34-35 0.05167639865420079 36-37 0.047054571091071765 38-39 0.05131745768252463 40-41 0.049082402559922605 42-43 0.047073073203013835 44-45 0.05068098503171743 46-47 0.04452348217739662 48-49 0.04006817362174623 50-51 0.04445687457440517 52-53 0.040264296008332166 54-55 0.04377229643254859 56-57 0.042540055777206744 58-59 0.0497965840808865 60-61 0.0486309510285361 62-63 0.053430398866308994 64-65 0.04949314944503655 66-67 0.04389070994897784 68-69 0.04385000530270528 70-71 0.052782824948336554 72-73 0.040264296008332166 74-75 0.04789086655085332 76-77 0.04649950773280967 78-79 0.044356963169917996 80-81 0.04437176485947165 82-83 0.04343185757281451 84-85 0.043935115017638804 86-87 0.04896028862110494 88-89 0.0416519544039874 90-91 0.044305157256480196 92-93 0.04082305978898267 94-95 0.04380560023404431 96-97 0.029599678684923168 98-99 0.03607911828703599 100-101 0.024859437605364902 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.3511971E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 39.46647523301839 #Duplication Level Percentage of deduplicated Percentage of total 1 88.8866763782026 35.08043811825654 2 5.7148804661882435 4.510923767569579 3 1.8952431283926774 2.243956979617737 4 0.9635972374389633 1.5211914602395917 5 0.5906629607433741 1.1655692555619843 6 0.3715768076531635 0.879889612584457 7 0.2816657755955216 0.7781448749570699 8 0.19051958796242208 0.601530927977904 9 0.14690252877320306 0.5217952512145851 >10 0.923704271089567 6.599032525914221 >50 0.017022509752720367 0.42360939759008354 >100 0.008754557145652611 0.7222872571782973 >500 0.0035008457488118197 1.0052237468490919 >1k 0.00425102694453657 3.3360190566786776 >5k 7.085044907560948E-4 1.9124153856261106 >10k+ 3.334138780028682E-4 38.69797238218408 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 2193808 16.236032478163253 No Hit AAGCAGTGGTATCAACGCAGAGTATTTTTTTTTTTTTTTTTTTTTTTTTT 50923 0.3768732185704069 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 34150 0.2527388491286726 No Hit AAGCAGTGGTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAG 25696 0.1901721073853696 No Hit AAGCAGTGGTATCAACGCAGAGTACCTTTTTTTTTTTTTTTTTTTTTTTT 14599 0.10804493289691046 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 2.2202534330483687E-5 0.0 0.0 0.0 0.0 2 3.700422388413948E-5 0.0 0.0 0.0 0.0 3 5.180591343779527E-5 0.0 0.0 0.0 0.0 4 5.180591343779527E-5 0.0 0.0 0.0 0.0 5 5.180591343779527E-5 0.0 0.0 0.0 0.0 6 5.180591343779527E-5 7.400844776827896E-6 0.0 0.0 0.0 7 6.660760299145106E-5 7.400844776827896E-6 0.0 0.0 0.0 8 7.400844776827896E-5 7.400844776827896E-6 0.0 0.0 0.0 9 9.621098209876264E-5 7.400844776827896E-6 0.0 0.0 0.0 10-11 9.621098209876264E-5 7.400844776827896E-6 0.0 0.0 7.400844776827896E-6 12-13 1.2211393881766028E-4 7.400844776827896E-6 0.0 0.0 7.400844776827896E-6 14-15 1.3321520598290213E-4 7.400844776827896E-6 0.0 0.0 7.400844776827896E-6 16-17 1.5541774031338582E-4 7.400844776827896E-6 0.0 7.400844776827896E-6 1.1101267165241845E-5 18-19 2.072236537511811E-4 7.400844776827896E-6 0.0 2.2202534330483687E-5 2.2202534330483687E-5 20-21 2.479283000237345E-4 7.400844776827896E-6 0.0 2.2202534330483687E-5 2.2202534330483687E-5 22-23 2.8123210151946E-4 7.400844776827896E-6 0.0 2.2202534330483687E-5 2.2202534330483687E-5 24-25 3.0713505823835765E-4 7.400844776827896E-6 0.0 2.2202534330483687E-5 2.2202534330483687E-5 26-27 3.478397045109111E-4 1.1101267165241845E-5 0.0 2.9603379107311584E-5 2.2202534330483687E-5 28-29 3.9224477317187844E-4 1.4801689553655792E-5 0.0 4.440506866096737E-5 2.2202534330483687E-5 30-31 4.181477298907761E-4 1.4801689553655792E-5 0.0 4.810549104938132E-5 2.2202534330483687E-5 32-33 4.884557552706411E-4 1.4801689553655792E-5 0.0 5.5506335826209215E-5 2.9603379107311584E-5 34-35 5.328608239316085E-4 2.2202534330483687E-5 0.0 6.290718060303712E-5 4.0704646272553426E-5 36-37 5.772658925925758E-4 2.2202534330483687E-5 0.0 6.660760299145106E-5 4.440506866096737E-5 38-39 6.438734955840269E-4 2.5902956718897637E-5 0.0 6.660760299145106E-5 4.810549104938132E-5 40-41 7.104810985754781E-4 2.9603379107311584E-5 0.0 7.400844776827896E-5 5.180591343779527E-5 42-43 7.659874344016872E-4 2.9603379107311584E-5 0.0 7.400844776827896E-5 5.920675821462317E-5 44-45 8.844009508309335E-4 2.9603379107311584E-5 0.0 7.400844776827896E-5 6.660760299145106E-5 46-47 9.99114044871766E-4 2.9603379107311584E-5 0.0 8.140929254510685E-5 7.400844776827896E-5 48-49 0.001065721647863217 2.9603379107311584E-5 0.0 8.881013732193475E-5 7.400844776827896E-5 50-51 0.0011619326299619796 2.9603379107311584E-5 0.0 1.0361182687559054E-4 8.881013732193475E-5 52-53 0.0012581436120607423 2.9603379107311584E-5 0.0 1.0361182687559054E-4 8.881013732193475E-5 54-55 0.0013765571284899887 2.9603379107311584E-5 0.0 1.0361182687559054E-4 8.881013732193475E-5 56-57 0.001494970644919235 3.700422388413948E-5 0.0 1.0361182687559054E-4 8.881013732193475E-5 58-59 0.0015652786702990999 3.700422388413948E-5 0.0 1.0361182687559054E-4 9.25105597103487E-5 60-61 0.0016910930315051742 3.700422388413948E-5 0.0 1.0361182687559054E-4 9.621098209876264E-5 62-63 0.0018428103494301461 4.0704646272553426E-5 0.0 1.0361182687559054E-4 9.621098209876264E-5 64-65 0.0019501225986941506 4.440506866096737E-5 0.0 1.0361182687559054E-4 9.621098209876264E-5 66-67 0.0021240424509496062 4.440506866096737E-5 0.0 1.0731224926400449E-4 1.0731224926400449E-4 68-69 0.0023164644151471314 4.810549104938132E-5 0.0 1.1841351642924634E-4 1.1841351642924634E-4 70-71 0.0025236880688983125 5.180591343779527E-5 0.0 1.1841351642924634E-4 1.2211393881766028E-4 72-73 0.0026939074987653543 5.180591343779527E-5 0.0 1.1841351642924634E-4 1.4061605075973E-4 74-75 0.0029381353764006747 5.5506335826209215E-5 0.0 1.1841351642924634E-4 1.4431647314814395E-4 76-77 0.0031564602973170974 5.920675821462317E-5 0.0 1.1841351642924634E-4 1.4801689553655791E-4 78-79 0.003478397045109111 5.920675821462317E-5 0.0 1.1841351642924634E-4 1.5541774031338582E-4 80-81 0.003900245197388301 5.920675821462317E-5 0.0 1.1841351642924634E-4 1.5541774031338582E-4 82-83 0.004447907710873566 5.920675821462317E-5 0.0 1.2581436120607422E-4 1.5541774031338582E-4 84-85 0.005287903593043532 5.920675821462317E-5 0.0 1.4061605075973E-4 1.5541774031338582E-4 86-87 0.006405431154344544 6.660760299145106E-5 0.0 1.5541774031338582E-4 1.5541774031338582E-4 88-89 0.007870798420156467 6.660760299145106E-5 0.0 1.5541774031338582E-4 1.5911816270179976E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AAGCAGT 463475 0.0 92.53406 1 AGTGGTA 463615 0.0 91.80217 5 AGCAGTG 467040 0.0 91.71801 2 GCAGTGG 467670 0.0 91.36855 3 GGTATCA 466415 0.0 91.31771 8 TGGTATC 466605 0.0 91.287964 7 GTATCAA 466610 0.0 91.255455 9 GTGGTAT 467570 0.0 91.213715 6 CAGTGGT 468830 0.0 90.79769 4 ATCAACG 467330 0.0 46.069504 10-11 CAACGCA 468285 0.0 46.030914 12-13 TCAACGC 467755 0.0 46.008926 12-13 AACGCAG 469795 0.0 45.901905 14-15 ACGCAGA 470760 0.0 45.87946 14-15 CGCAGAG 471890 0.0 45.76121 16-17 TATCAAC 469050 0.0 45.73951 10-11 AGAGTAC 459865 0.0 45.68542 18-19 CAGAGTA 468340 0.0 45.57166 18-19 GCAGAGT 472655 0.0 45.451992 16-17 GAGTACT 268575 0.0 45.43427 20-21 >>END_MODULE