FastQCFastQC Report
Sat 18 Jun 2016
SRR578607_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR578607_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13511971
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4167803.0845240860863306No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT308310.22817544531438086No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT301280.22297265143627082No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT160300.028.426911
AGTGGTA175900.024.8967085
GGTATCA188000.023.1928258
CGTCACG10400.022.8356573
GTATCAA193450.022.5394219
CGCGTAG5700.022.08325854-55
AGCAGTG212350.021.5402572
TGGTATC204700.021.3935017
GTGGTAT213200.020.8749776
ACTCGCG6250.019.76121150-51
GCAGTGG227700.019.6709583
ATCAACG173450.019.64787710-11
CGCTAGT6600.019.43124890-91
CTCGCGT6400.019.29759252-53
CAACGCA176650.019.27847512-13
ACGTACG6250.018.99905620-21
CAGTGGT244250.018.299264
ACGCAGA197500.017.3033814-15
AGAGTAC207750.015.9468618-19
CGTAGCG8050.015.04594856-57
Produced by FastQC (version 0.11.5)