##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR578607_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 13511971 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.46942670318046 31.0 31.0 34.0 30.0 34.0 2 31.784964532561535 31.0 31.0 34.0 30.0 34.0 3 31.85874518232758 31.0 31.0 34.0 30.0 34.0 4 35.527016450819794 37.0 35.0 37.0 33.0 37.0 5 35.3445157630963 37.0 35.0 37.0 33.0 37.0 6 35.33866139884403 37.0 35.0 37.0 32.0 37.0 7 35.31206579706247 37.0 35.0 37.0 32.0 37.0 8 35.298964599613186 37.0 35.0 37.0 32.0 37.0 9 36.86228907684897 39.0 37.0 39.0 32.0 39.0 10-11 36.82615123285862 39.0 37.0 39.0 32.0 39.0 12-13 36.80711226363644 39.0 37.0 39.0 32.0 39.0 14-15 37.98058936035312 40.0 37.0 41.0 33.0 41.0 16-17 37.91047749436407 40.0 37.0 41.0 33.0 41.0 18-19 37.77659221589508 40.0 36.5 41.0 32.0 41.0 20-21 37.6864934434806 39.5 36.0 41.0 32.0 41.0 22-23 37.58228237020343 39.0 36.0 41.0 32.0 41.0 24-25 37.48555584525751 39.0 36.0 41.0 32.0 41.0 26-27 37.29386919199278 39.0 36.0 41.0 31.5 41.0 28-29 37.11400364906052 39.0 36.0 41.0 31.0 41.0 30-31 36.97404975188298 39.0 35.5 40.5 31.0 41.0 32-33 36.835624980248994 39.0 35.0 40.0 30.5 41.0 34-35 36.665871322547986 38.5 35.0 40.0 30.0 41.0 36-37 36.49881986129189 38.0 35.0 40.0 30.0 41.0 38-39 36.3100712323909 38.0 35.0 40.0 30.0 41.0 40-41 36.1244413934873 38.0 35.0 40.0 30.0 41.0 42-43 36.038423224857425 38.0 35.0 40.0 29.5 41.0 44-45 36.04760138250741 38.0 35.0 40.0 29.5 41.0 46-47 35.89897721065269 38.0 34.5 40.0 29.0 41.0 48-49 35.73002643359729 38.0 34.0 40.0 28.0 41.0 50-51 35.50269368547342 38.0 34.0 40.0 28.0 41.0 52-53 35.526383863612494 38.0 34.0 40.0 28.0 41.0 54-55 35.491278622489645 38.0 34.0 40.0 28.0 41.0 56-57 35.30738365261441 38.0 34.0 40.0 28.0 41.0 58-59 35.08093619354275 37.0 34.0 40.0 27.0 41.0 60-61 34.80439289723165 37.0 33.0 40.0 26.5 41.0 62-63 34.507083015497884 36.5 33.0 40.0 26.0 41.0 64-65 34.17309798844299 36.0 33.0 39.0 26.0 41.0 66-67 33.82161547712025 35.5 32.5 39.0 26.0 41.0 68-69 33.4515736083211 35.0 32.0 39.0 26.0 40.0 70-71 33.06698171569492 35.0 32.0 38.0 25.0 40.0 72-73 32.67771866887517 35.0 31.0 37.0 24.5 39.5 74-75 32.280522212488464 35.0 31.0 37.0 24.0 39.0 76-77 30.96096528034289 33.5 29.5 35.5 21.5 38.5 78-79 31.612595934375527 34.0 31.0 36.0 23.0 38.5 80-81 31.510288099345388 34.0 31.0 35.5 23.0 37.0 82-83 31.218313449607017 34.0 31.0 35.0 22.5 37.0 84-85 30.9098613740364 34.0 31.0 35.0 20.5 36.5 86-87 30.57687102051951 34.0 30.0 35.0 20.0 36.0 88-89 30.26005280798782 34.0 30.0 35.0 19.0 36.0 90-91 29.939619985862905 34.0 30.0 35.0 18.0 35.5 92-93 29.574406724229945 34.0 29.5 35.0 13.0 35.0 94-95 29.19545312819277 34.0 29.0 35.0 4.5 35.0 96-97 28.792961219351344 34.0 29.0 35.0 2.0 35.0 98-99 28.295359093059034 34.0 29.0 35.0 2.0 35.0 100-101 27.16211432070125 33.0 27.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.339085998915742 1101 2 0.39333436378771935 1101 3 0.3576929437465246 1101 4 0.2589542844823072 1101 5 0.34644175498904417 1101 6 0.30275811318443857 1101 7 0.30173486140213157 1101 8 0.22935332515483253 1101 9 0.35672888515630063 1101 10-11 0.3932218395695415 1101 12-13 0.37332806835563304 1101 14-15 0.49784010029180337 1101 16-17 0.4611360288445425 1101 18-19 0.3824739211970396 1101 20-21 0.40253287479938393 1101 22-23 0.5076705321711401 1101 24-25 0.5213711197509099 1101 26-27 0.5068164191946423 1101 28-29 0.5508958566803059 1101 30-31 0.5383901281780936 1101 32-33 0.4849183613934045 1101 34-35 0.5435489792174195 1101 36-37 0.4716546465272984 1101 38-39 0.5093875377443027 1101 40-41 0.5282579950003168 1101 42-43 0.4661644274392742 1101 44-45 0.45696534952301704 1101 46-47 0.4371111275994437 1101 48-49 0.4210197520783723 1101 50-51 0.3658525032849198 1101 52-53 0.473946534964206 1101 54-55 0.47490212168055024 1101 56-57 0.4453584449774297 1101 58-59 0.39437283271794144 1101 60-61 0.44528397965046906 1101 62-63 0.3643511277469216 1101 64-65 0.4251312169660295 1101 66-67 0.4711670621394788 1101 68-69 0.5132692348936203 1101 70-71 0.4882761126220245 1101 72-73 0.47699152478779183 1101 74-75 0.35897321448578623 1101 76-77 0.346641980807064 1101 78-79 0.5106011250716271 1101 80-81 0.45549860524305785 1101 82-83 0.4534413011411047 1101 84-85 0.571140723095585 1101 86-87 0.48456253641323244 1101 88-89 0.3822605335683562 1101 90-91 0.5297831547450293 1101 92-93 0.5570183567745417 1101 94-95 0.6158003595130594 1101 96-97 0.5555580126620967 1101 98-99 0.6714687171027265 1101 100-101 0.7045898784525235 1102 1 0.27166177844608796 1102 2 0.2770766749692619 1102 3 0.3172216227360529 1102 4 0.2602058745297242 1102 5 0.2977874797841409 1102 6 0.2037252612370608 1102 7 0.19620533440295418 1102 8 0.20976348241661213 1102 9 0.3612554559670471 1102 10-11 0.3229930980377489 1102 12-13 0.26409348356047957 1102 14-15 0.44177854475528733 1102 16-17 0.387799059145685 1102 18-19 0.42576277849260435 1102 20-21 0.41601262304796904 1102 22-23 0.3664477839046967 1102 24-25 0.36912419617254955 1102 26-27 0.39398943642027007 1102 28-29 0.4012012977800268 1102 30-31 0.36034550806492405 1102 32-33 0.37280569416450504 1102 34-35 0.39923978766935164 1102 36-37 0.4043150147447605 1102 38-39 0.5291005515798233 1102 40-41 0.41840903538722074 1102 42-43 0.39763798559035735 1102 44-45 0.3934816864454902 1102 46-47 0.3567316321974445 1102 48-49 0.3752877770538561 1102 50-51 0.35899572964745374 1102 52-53 0.4414932627225383 1102 54-55 0.46933047743163314 1102 56-57 0.5065089644153034 1102 58-59 0.5375728833268951 1102 60-61 0.5107209875605463 1102 62-63 0.3948980847068668 1102 64-65 0.4535574988898432 1102 66-67 0.46638339449621213 1102 68-69 0.40460773918665893 1102 70-71 0.4078272544965884 1102 72-73 0.43929962970694447 1102 74-75 0.36143255799618146 1102 76-77 0.3404158341571275 1102 78-79 0.376742659109194 1102 80-81 0.38374304817093474 1102 82-83 0.39096062239936913 1102 84-85 0.2844410342564636 1102 86-87 0.26379618143833383 1102 88-89 0.40695307963904526 1102 90-91 0.48504415415066404 1102 92-93 0.42090663583649857 1102 94-95 0.25412365387469293 1102 96-97 0.24806186817633957 1102 98-99 0.3517475956541176 1102 100-101 0.5192810692277305 1103 1 0.21090132390472505 1103 2 0.24689423983902614 1103 3 0.2752365407134043 1103 4 0.12678623584549342 1103 5 0.2388705108282494 1103 6 0.15099452209574338 1103 7 0.14596706756411493 1103 8 0.1189722800373616 1103 9 0.325564762499404 1103 10-11 0.23084429610572954 1103 12-13 0.22986668614090178 1103 14-15 0.35824848178945956 1103 16-17 0.28735313255214834 1103 18-19 0.2983594426106251 1103 20-21 0.29421527338610076 1103 22-23 0.319066395324235 1103 24-25 0.32780661478250295 1103 26-27 0.25102743088233836 1103 28-29 0.2869719181233563 1103 30-31 0.3105031054149805 1103 32-33 0.31384282029480204 1103 34-35 0.28551020947221417 1103 36-37 0.37232184897874987 1103 38-39 0.42369091340331266 1103 40-41 0.3667239011897223 1103 42-43 0.2772662245658992 1103 44-45 0.20086609049208448 1103 46-47 0.2482835011626534 1103 48-49 0.2661564019889582 1103 50-51 0.30686050336822035 1103 52-53 0.32284550654969735 1103 54-55 0.3067433100783177 1103 56-57 0.2950422094210836 1103 58-59 0.31983263576756116 1103 60-61 0.31474470177853675 1103 62-63 0.22394392422501852 1103 64-65 0.3094144929044731 1103 66-67 0.27873306390086583 1103 68-69 0.2889276134460417 1103 70-71 0.20545327696627425 1103 72-73 0.21343653735361556 1103 74-75 0.24198073464340553 1103 76-77 0.17697044599234957 1103 78-79 0.28490422946214267 1103 80-81 0.3595171983256549 1103 82-83 0.2637316920578918 1103 84-85 0.35151452836621644 1103 86-87 0.4419235616560968 1103 88-89 0.4575339138965333 1103 90-91 0.47988111950179757 1103 92-93 0.3345498218541323 1103 94-95 0.38832620669058926 1103 96-97 0.315661583634423 1103 98-99 0.4456494614592259 1103 100-101 0.5793005844142591 1104 1 0.1443031539796955 1104 2 0.1975260697480543 1104 3 0.19975546302780955 1104 4 0.1647123769140677 1104 5 0.1901484665574671 1104 6 0.22206559657506375 1104 7 0.12661376570558502 1104 8 0.07673601100439953 1104 9 0.22680148112521437 1104 10-11 0.25766320067310744 1104 12-13 0.22903807644151186 1104 14-15 0.2424622924021591 1104 16-17 0.26300833298128623 1104 18-19 0.27758105911697584 1104 20-21 0.29745522171543115 1104 22-23 0.3610810634095216 1104 24-25 0.31781268586172473 1104 26-27 0.3377278319986843 1104 28-29 0.3168839773337737 1104 30-31 0.2286812087775303 1104 32-33 0.22256259555522462 1104 34-35 0.26440558962742244 1104 36-37 0.3067985444728265 1104 38-39 0.3711573543193154 1104 40-41 0.3909161414387725 1104 42-43 0.23958513540302562 1104 44-45 0.2890686751892986 1104 46-47 0.30052567940729347 1104 48-49 0.23106583793163082 1104 50-51 0.26173379976754774 1104 52-53 0.3506931156406452 1104 54-55 0.32114380431089984 1104 56-57 0.3888592862406881 1104 58-59 0.4185176224008771 1104 60-61 0.4393810287400157 1104 62-63 0.3444538164247746 1104 64-65 0.4804681608392585 1104 66-67 0.4549635122258806 1104 68-69 0.3682233353103257 1104 70-71 0.20364568549585726 1104 72-73 0.3006186734324885 1104 74-75 0.38598835731491477 1104 76-77 0.3428078170335702 1104 78-79 0.37404988811434947 1104 80-81 0.44661287466428234 1104 82-83 0.32886560543013843 1104 84-85 0.38034790411126806 1104 86-87 0.46033352510621484 1104 88-89 0.4148995956350845 1104 90-91 0.5503372295889974 1104 92-93 0.6633161134795671 1104 94-95 0.5712435884007405 1104 96-97 0.6952441785091281 1104 98-99 0.7291449085960799 1104 100-101 0.7535684219792955 1105 1 0.1510906323055643 1105 2 0.1495006149143947 1105 3 0.09261453371157913 1105 4 0.11726156674992438 1105 5 0.11087536372482276 1105 6 0.20244111997211434 1105 7 0.16971181650161782 1105 8 0.163444988019954 1105 9 0.17286721141935146 1105 10-11 0.06680016753343665 1105 12-13 0.12371770884710287 1105 14-15 0.1638577094151188 1105 16-17 0.09937277008301493 1105 18-19 0.14210802278225998 1105 20-21 0.1578129004768556 1105 22-23 0.1576087335313403 1105 24-25 0.1796319461167215 1105 26-27 0.19556824457752953 1105 28-29 0.16186500888553468 1105 30-31 0.1773055537209558 1105 32-33 0.17859235029323628 1105 34-35 0.23920899280978603 1105 36-37 0.27446666471727355 1105 38-39 0.2771682342163686 1105 40-41 0.2800417683711487 1105 42-43 0.24427624410274262 1105 44-45 0.13038488994048691 1105 46-47 0.11382790479790117 1105 48-49 0.21683847218158547 1105 50-51 0.2500279209322187 1105 52-53 0.20524419373802516 1105 54-55 0.25304010608596883 1105 56-57 0.16152492773082372 1105 58-59 0.13645681503003004 1105 60-61 0.06138684609081224 1105 62-63 0.13746524897246104 1105 64-65 0.18928703683166503 1105 66-67 0.10591892419098059 1105 68-69 0.15641088863548447 1105 70-71 0.09387265036302495 1105 72-73 0.1317921090363754 1105 74-75 0.18635278063295857 1105 76-77 0.21510304476021602 1105 78-79 0.15989563374452942 1105 80-81 0.2042382457998002 1105 82-83 0.2147879829566257 1105 84-85 0.31717486674278206 1105 86-87 0.33303124573646414 1105 88-89 0.34707633314897635 1105 90-91 0.2690244058165554 1105 92-93 0.2494332608533938 1105 94-95 0.2714632046239345 1105 96-97 0.3528014537654087 1105 98-99 0.5319592922586658 1105 100-101 0.534639077634079 1106 1 -0.06401051179884121 1106 2 0.01893097637862695 1106 3 -0.05541836340443851 1106 4 0.04758827476410232 1106 5 0.01727359260335959 1106 6 0.12716034615756655 1106 7 0.1080261631824655 1106 8 0.11027053080135829 1106 9 0.015530772260525794 1106 10-11 -0.004097669009674121 1106 12-13 -0.011292773585374505 1106 14-15 0.20207458114097676 1106 16-17 0.13190111891425005 1106 18-19 0.007845815621756458 1106 20-21 0.11926078827182351 1106 22-23 0.06872954988877211 1106 24-25 0.1472620920221459 1106 26-27 0.22561705667710186 1106 28-29 0.20002064207312742 1106 30-31 0.09991469963264166 1106 32-33 0.07311519150422896 1106 34-35 0.005377071341762019 1106 36-37 0.10613970828927677 1106 38-39 0.04833198935172334 1106 40-41 -0.024072352182443524 1106 42-43 -0.018298744165306857 1106 44-45 0.022808250725518064 1106 46-47 0.017456744465697227 1106 48-49 0.07237826933813096 1106 50-51 0.057533354079410515 1106 52-53 -0.12136444587191875 1106 54-55 -0.07481271028746761 1106 56-57 -0.07728979776616995 1106 58-59 0.08538191009174767 1106 60-61 0.15356318118834622 1106 62-63 0.017544066385632107 1106 64-65 -0.014194161832655539 1106 66-67 -0.06879193495418434 1106 68-69 -0.03429104249445203 1106 70-71 0.0032300038651982277 1106 72-73 -0.09295179885345561 1106 74-75 -0.2004478279425541 1106 76-77 -0.2287994522669301 1106 78-79 -0.06313854504025684 1106 80-81 0.059441096601592136 1106 82-83 0.04983491311083199 1106 84-85 0.1267574735310184 1106 86-87 0.2884217290137272 1106 88-89 0.1975287196438984 1106 90-91 0.2652386527401376 1106 92-93 0.16912470750699526 1106 94-95 0.3550853986688409 1106 96-97 0.29244869604640655 1106 98-99 0.3526697965178265 1106 100-101 0.34490651229994995 1107 1 0.08936377578133659 1107 2 0.0776761084009685 1107 3 0.049591799980266416 1107 4 0.11681309678748875 1107 5 0.026774795365192006 1107 6 -0.020236074146566807 1107 7 0.062196039894203636 1107 8 0.022548336646082134 1107 9 -0.05201048546469167 1107 10-11 -0.02140887807841807 1107 12-13 -0.05037735272522781 1107 14-15 -0.03881995799258675 1107 16-17 0.115133335535063 1107 18-19 6.503982329704172E-4 1107 20-21 -0.030732414842553624 1107 22-23 -0.07515896832632762 1107 24-25 -0.07150082089646048 1107 26-27 0.0035184932565854865 1107 28-29 0.002506358533359787 1107 30-31 0.0027559783507200564 1107 32-33 0.007297206622837393 1107 34-35 -0.0052655238458356735 1107 36-37 0.012372944825322918 1107 38-39 0.04143331822987051 1107 40-41 0.001008878911804345 1107 42-43 -0.038401244186729855 1107 44-45 -0.0404114738183452 1107 46-47 -0.023514618802892073 1107 48-49 0.00653041395363374 1107 50-51 0.06741066817392749 1107 52-53 0.035451809142379886 1107 54-55 -0.011841847640504 1107 56-57 -0.013513232729600588 1107 58-59 0.019999717719805687 1107 60-61 0.19602420410971888 1107 62-63 0.15636544901268934 1107 64-65 0.15964173773404156 1107 66-67 0.06788370347664596 1107 68-69 0.1381802617797021 1107 70-71 0.11692606889737078 1107 72-73 0.04574335143846042 1107 74-75 0.050097815338567386 1107 76-77 0.014074440160541712 1107 78-79 0.048545694914480464 1107 80-81 0.143977903218655 1107 82-83 0.23344897636740214 1107 84-85 0.21352549721649083 1107 86-87 0.2191845821236953 1107 88-89 0.19689178312565758 1107 90-91 0.14292026655661516 1107 92-93 0.1797725572810549 1107 94-95 0.09860002028141679 1107 96-97 0.17691895722791884 1107 98-99 0.23954882256181165 1107 100-101 0.4026217401742187 1108 1 0.07308925285739676 1108 2 0.055643908444988455 1108 3 0.02135451210671846 1108 4 0.056761953599462345 1108 5 -0.04482776672208644 1108 6 0.01084248716981051 1108 7 0.06508771542225134 1108 8 0.05979796077733823 1108 9 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-1.5594589770267575 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 276.0 3 228.0 4 675.0 5 1731.0 6 2955.0 7 4780.0 8 7464.0 9 10717.0 10 14743.0 11 18568.0 12 23211.0 13 27548.0 14 31328.0 15 35856.0 16 41504.0 17 48865.0 18 56692.0 19 65203.0 20 69609.0 21 72838.0 22 80631.0 23 95849.0 24 118182.0 25 147859.0 26 185739.0 27 235091.0 28 291378.0 29 358872.0 30 435697.0 31 526071.0 32 639302.0 33 793696.0 34 1033751.0 35 1517098.0 36 1767155.0 37 2341964.0 38 2004824.0 39 403462.0 40 559.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 17.353216908855934 31.053498304120485 28.128876022065448 23.464408764958133 2 22.78851101737859 30.571106169484825 26.297532758174214 20.342850054962376 3 22.344415925700257 28.99366791121739 26.531658482689163 22.130257680393186 4 22.02411019256344 28.760363265583134 27.057353240158218 22.158173301695207 5 22.48293266873521 29.01952164476934 27.476551268601625 21.020994417893824 6 22.564431430348705 29.026864947015007 26.206043660210725 22.20265996242556 7 22.723738823891793 29.031900675334487 27.091895031450257 21.152465469323463 8 22.090274783513788 29.35099059750535 26.64798245937336 21.910752159607505 9 21.602509782096266 29.09218742252217 27.40786845402544 21.89743434135612 10-11 21.609800562235254 29.589409293300502 26.681973846946477 22.118816297517764 12-13 21.615328602367278 29.690228715000757 26.46462184748944 22.229820835142533 14-15 21.84372078978861 29.79196593834948 26.28293653701126 22.081376734850654 16-17 22.054222217255433 29.458190424557245 26.349217532705538 22.13836982548178 18-19 21.913316260568685 29.629826711414154 26.36449210326045 22.092364924756712 20-21 22.057126122764405 29.507135973295366 26.452771995658363 21.982965908281862 22-23 21.957128914987926 29.401294454072545 26.594223883730777 22.04735274720875 24-25 21.919019879446893 29.39517962979567 26.49111763528495 22.194682855472486 26-27 22.14040053815983 29.23163097374913 26.726763253118293 21.90120523497275 28-29 22.134457546831168 29.30107435360635 26.636620716126195 21.927847383436283 30-31 22.03396754787064 29.356271294845776 26.600971641114025 22.008789516169557 32-33 22.08239681525881 29.382856483122694 26.522732133751433 22.012014567867062 34-35 22.07493145597091 29.409421026321507 26.521167156252336 21.99448036145525 36-37 22.08053386299856 29.422349033605926 26.483653594737866 22.013463508657644 38-39 22.107551798185515 29.418068557036936 26.452856259503253 22.021523385274293 40-41 22.068909958053975 29.45403187299914 26.44844342393336 22.028614745013527 42-43 22.104523926554677 29.47348306232102 26.39461958777601 22.027373423348298 44-45 22.102309855727594 29.500208501325627 26.399302185713346 21.998179457233434 46-47 22.067156358858085 29.578354247740968 26.347746143265994 22.006743250134953 48-49 22.150448985485717 29.550147077528234 26.302558609063688 21.99684532792236 50-51 22.164352663516773 29.53756512036918 26.2900239896455 22.008058226468545 52-53 22.10663286545808 29.606936604859264 26.29142721399193 21.995003315690727 54-55 22.131551876538662 29.60545953291528 26.18078415470147 22.08220443584459 56-57 22.13850962231935 29.65807505063473 26.184699478706698 22.018715848339223 58-59 22.11316496576053 29.75383409274055 26.133557411285384 21.99944353021354 60-61 22.097665651618893 29.747454520177058 26.116673124581407 22.038206703622638 62-63 22.10268035937456 29.79048744830793 26.10113327447395 22.005698917843567 64-65 22.081387658380137 29.8324104872341 26.157831013349814 21.92837084103595 66-67 22.049523713946616 29.84755538094392 26.12844220429406 21.974478700815403 68-69 22.051538521599767 29.90941274815745 26.112197196013714 21.926851534229066 70-71 22.069358813070806 29.941265819449708 26.03581156438089 21.9535638030986 72-73 22.027593566075087 29.937169673410192 26.040096722615512 21.99514003789921 74-75 22.120368688723396 29.965986983608357 25.982657047284146 21.9309872803841 76-77 22.009050345060686 30.079741882216886 26.021048298579093 21.890159474143335 78-79 22.073607914048957 30.08847857947593 25.926173168962546 21.911740337512565 80-81 22.034180603608146 30.116848689955194 25.951328384113587 21.897642322323076 82-83 22.06240483654857 30.194517822909273 25.897895044678787 21.845182295863378 84-85 22.03047058006388 30.208719295654667 25.914824368633948 21.845985755647508 86-87 22.030664420446524 30.261873373502556 25.847732548081414 21.859729657969503 88-89 22.009105029784234 30.316674083044308 25.841121763352486 21.833099123818968 90-91 21.985300060124814 30.29928377165833 25.856552879493115 21.85886328872374 92-93 22.02003086539104 30.350200628742186 25.804954485482146 21.82481402038463 94-95 21.939105830445886 30.413144409971217 25.801591393208405 21.846158366374496 96-97 21.946839616122073 30.43008737332182 25.78249939543797 21.84057361511814 98-99 21.94406352707536 30.46717980670622 25.734417280794936 21.854339385423486 100-101 22.01740464260225 30.433542645282024 25.67559081597986 21.87346189613587 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 440146.0 1 243001.5 2 33085.5 3 18099.5 4 14803.0 5 12833.5 6 12304.5 7 12549.0 8 11655.5 9 10662.0 10 10955.5 11 13632.0 12 26955.0 13 25558.0 14 12495.0 15 12378.0 16 13246.5 17 14742.5 18 17529.5 19 19834.0 20 22740.0 21 28451.5 22 36047.0 23 45031.0 24 55547.0 25 67559.5 26 82644.0 27 98672.0 28 111597.5 29 124817.5 30 142945.0 31 165690.5 32 187208.5 33 207740.5 34 230703.5 35 250683.0 36 272274.5 37 297142.5 38 316513.5 39 328198.0 40 347486.0 41 371872.0 42 390550.0 43 411108.5 44 427072.0 45 443634.0 46 458516.5 47 467890.0 48 482585.0 49 494080.0 50 494035.5 51 489814.0 52 488848.0 53 480510.5 54 455210.5 55 422250.5 56 382066.5 57 340220.5 58 314392.5 59 288666.0 60 247729.0 61 204974.0 62 164398.5 63 126435.5 64 98534.5 65 76730.5 66 59562.0 67 49410.0 68 39291.5 69 30633.5 70 25286.0 71 21945.5 72 20005.5 73 16703.5 74 13057.0 75 10362.0 76 7905.0 77 5573.5 78 3790.5 79 2523.0 80 1700.5 81 1318.5 82 1082.5 83 905.5 84 802.5 85 652.0 86 451.5 87 322.5 88 184.5 89 98.0 90 73.5 91 45.5 92 25.0 93 17.0 94 12.5 95 9.5 96 6.5 97 3.5 98 1.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1447753255243073 2 0.0 3 0.0 4 0.003137958185375028 5 3.404388597340832E-4 6 9.325064418803148E-4 7 0.0 8 1.850211194206974E-4 9 3.0343463584994376E-4 10-11 8.51097149335208E-5 12-13 1.4801689553655792E-5 14-15 7.400844776827896E-6 16-17 3.700422388413948E-6 18-19 1.4801689553655792E-5 20-21 6.660760299145106E-5 22-23 1.4801689553655791E-4 24-25 3.700422388413948E-6 26-27 0.0 28-29 0.005750456391595275 30-31 0.001420962197150956 32-33 3.0343463584994376E-4 34-35 2.627299895773903E-4 36-37 2.9603379107311583E-4 38-39 0.0011545317851851518 40-41 0.003260072124192688 42-43 5.143587119895387E-4 44-45 4.92156177659055E-4 46-47 0.006146401587155567 48-49 0.006657059876756692 50-51 0.00690868859916884 52-53 0.008966123447126996 54-55 0.00467733389895523 56-57 0.0 58-59 4.810549104938133E-5 60-61 9.436077090455566E-4 62-63 7.955908135089988E-4 64-65 4.2554857466760396E-4 66-67 5.957680045346457E-4 68-69 0.0010065148896485937 70-71 0.001202637276234533 72-73 0.0025384897584519684 74-75 6.290718060303712E-4 76-77 0.0 78-79 0.0 80-81 7.030802537986502E-5 82-83 1.5911816270179976E-4 84-85 1.4431647314814397E-4 86-87 0.002838223971913498 88-89 0.0015430761359686164 90-91 5.846667373694038E-4 92-93 5.439620910968504E-4 94-95 2.7383125674263213E-4 96-97 9.140043299382452E-4 98-99 0.0 100-101 1.1101267165241843E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.3511971E7 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.59204074139613 #Duplication Level Percentage of deduplicated Percentage of total 1 89.67350488060191 52.54153651388012 2 5.547275987767624 6.500524413580982 3 1.4185875663333418 2.4935382144552345 4 0.7748286180049987 1.8159515981499417 5 0.5242831104865403 1.535940868482663 6 0.342799257439299 1.2051184834802249 7 0.24815339807778722 1.017786980720272 8 0.19050387217864154 0.8929608512067748 9 0.13046458003956649 0.6879767390088674 >10 1.0486123114563175 12.426803671665972 >50 0.0913030188831538 3.50645031093825 >100 0.00891786592641976 0.7413592184090315 >500 2.8707480198606654E-4 0.10935997399719209 >1k 1.9138320096605605E-4 0.2191684889641982 >5k 4.784580024151401E-5 0.25903259631992726 >10k+ 2.3922900120757008E-4 14.046491076740383 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 416780 3.0845240860863306 No Hit AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 30831 0.22817544531438086 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 30128 0.22297265143627082 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 2.2202534330483687E-5 0.0 0.0 0.0 0.0 2 4.440506866096737E-5 0.0 0.0 0.0 0.0 3 5.920675821462317E-5 0.0 0.0 0.0 7.400844776827896E-6 4 6.660760299145106E-5 7.400844776827896E-6 0.0 0.0 1.4801689553655792E-5 5 7.400844776827896E-5 1.4801689553655792E-5 0.0 7.400844776827896E-6 1.4801689553655792E-5 6 8.140929254510685E-5 1.4801689553655792E-5 0.0 7.400844776827896E-6 1.4801689553655792E-5 7 8.881013732193475E-5 1.4801689553655792E-5 0.0 7.400844776827896E-6 1.4801689553655792E-5 8 9.621098209876264E-5 1.4801689553655792E-5 0.0 7.400844776827896E-6 2.2202534330483687E-5 9 1.2581436120607422E-4 1.4801689553655792E-5 0.0 7.400844776827896E-6 2.2202534330483687E-5 10-11 1.3691562837131607E-4 2.2202534330483687E-5 0.0 2.9603379107311584E-5 2.9603379107311584E-5 12-13 1.5171731792497188E-4 2.2202534330483687E-5 0.0 3.700422388413948E-5 4.0704646272553426E-5 14-15 1.6651900747862764E-4 2.5902956718897637E-5 0.0 3.700422388413948E-5 4.440506866096737E-5 16-17 2.0352323136276713E-4 2.9603379107311584E-5 0.0 3.700422388413948E-5 5.920675821462317E-5 18-19 2.627299895773903E-4 2.9603379107311584E-5 0.0 4.440506866096737E-5 7.770887015669291E-5 20-21 2.84932523907874E-4 2.9603379107311584E-5 0.0 4.440506866096737E-5 8.140929254510685E-5 22-23 3.1453590301518553E-4 2.9603379107311584E-5 0.0 4.440506866096737E-5 9.25105597103487E-5 24-25 3.700422388413948E-4 2.9603379107311584E-5 0.0 4.440506866096737E-5 1.0361182687559054E-4 26-27 4.5145153138650165E-4 2.9603379107311584E-5 0.0 4.440506866096737E-5 1.1101267165241844E-4 28-29 5.217595567663666E-4 2.9603379107311584E-5 0.0 4.440506866096737E-5 1.1101267165241844E-4 30-31 5.6246420303892E-4 2.9603379107311584E-5 0.0 4.440506866096737E-5 1.1841351642924634E-4 32-33 6.031688493114734E-4 2.9603379107311584E-5 0.0 4.440506866096737E-5 1.2211393881766028E-4 34-35 6.549747627492687E-4 2.9603379107311584E-5 0.0 4.810549104938132E-5 1.2581436120607422E-4 36-37 7.215823657407198E-4 3.330380149572553E-5 0.0 5.180591343779527E-5 1.4061605075973E-4 38-39 7.770887015669291E-4 3.700422388413948E-5 0.0 5.180591343779527E-5 1.4061605075973E-4 40-41 8.325950373931383E-4 3.700422388413948E-5 0.0 5.180591343779527E-5 1.4431647314814395E-4 42-43 8.881013732193474E-4 3.700422388413948E-5 0.0 5.180591343779527E-5 1.5541774031338582E-4 44-45 0.0010139157344254217 3.700422388413948E-5 0.0 5.180591343779527E-5 1.628185850902137E-4 46-47 0.001132329250854668 3.700422388413948E-5 0.0 5.180591343779527E-5 1.628185850902137E-4 48-49 0.0012396415001186725 3.700422388413948E-5 0.0 6.290718060303712E-5 1.6651900747862764E-4 50-51 0.0013321520598290212 3.700422388413948E-5 0.0 6.660760299145106E-5 1.702194298670416E-4 52-53 0.0014357638867046117 3.700422388413948E-5 0.0 6.660760299145106E-5 1.7391985225545555E-4 54-55 0.0015430761359686164 3.700422388413948E-5 0.0 7.0308025379865E-5 1.776202746438695E-4 56-57 0.0016762913419515182 3.700422388413948E-5 0.0 8.140929254510685E-5 1.850211194206974E-4 58-59 0.0017577006344966254 3.700422388413948E-5 0.0 8.881013732193475E-5 2.072236537511811E-4 60-61 0.0019131183748100113 3.700422388413948E-5 0.0 9.621098209876264E-5 2.1462449852800898E-4 62-63 0.0021055403390075364 3.700422388413948E-5 0.0 9.621098209876264E-5 2.1832492091642292E-4 64-65 0.0022165530106599547 3.700422388413948E-5 0.0 1.0361182687559054E-4 2.2942618808166477E-4 66-67 0.002375671173361755 3.700422388413948E-5 0.0 1.0361182687559054E-4 2.4422787763532056E-4 68-69 0.002545890603228796 4.0704646272553426E-5 0.0 1.1101267165241844E-4 2.4422787763532056E-4 70-71 0.0027235108778726654 4.440506866096737E-5 0.0 1.1101267165241844E-4 2.4422787763532056E-4 72-73 0.0028604265062439817 4.440506866096737E-5 0.0 1.147130940408324E-4 2.5162872241214844E-4 74-75 0.0030750510047719906 4.440506866096737E-5 0.0 1.2211393881766028E-4 2.5902956718897637E-4 76-77 0.0032822746585231717 4.440506866096737E-5 0.0 1.2581436120607422E-4 2.701308343542182E-4 78-79 0.003578308449596288 4.440506866096737E-5 0.0 1.4061605075973E-4 2.84932523907874E-4 80-81 0.003959451955602924 4.440506866096737E-5 0.0 1.4061605075973E-4 2.8863294629628795E-4 82-83 0.00449971362431136 4.440506866096737E-5 0.0 1.4061605075973E-4 2.9603379107311583E-4 84-85 0.00527680232587829 4.440506866096737E-5 0.0 1.5171731792497188E-4 2.9603379107311583E-4 86-87 0.006375827775237233 4.440506866096737E-5 0.0 1.5911816270179976E-4 3.1083548062677165E-4 88-89 0.007811591661941844 4.440506866096737E-5 0.0 1.6651900747862764E-4 3.330380149572553E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AAGCAGT 16030 0.0 28.42691 1 AGTGGTA 17590 0.0 24.896708 5 GGTATCA 18800 0.0 23.192825 8 CGTCACG 1040 0.0 22.835657 3 GTATCAA 19345 0.0 22.539421 9 CGCGTAG 570 0.0 22.083258 54-55 AGCAGTG 21235 0.0 21.540257 2 TGGTATC 20470 0.0 21.393501 7 GTGGTAT 21320 0.0 20.874977 6 ACTCGCG 625 0.0 19.761211 50-51 GCAGTGG 22770 0.0 19.670958 3 ATCAACG 17345 0.0 19.647877 10-11 CGCTAGT 660 0.0 19.431248 90-91 CTCGCGT 640 0.0 19.297592 52-53 CAACGCA 17665 0.0 19.278475 12-13 ACGTACG 625 0.0 18.999056 20-21 CAGTGGT 24425 0.0 18.29926 4 ACGCAGA 19750 0.0 17.30338 14-15 AGAGTAC 20775 0.0 15.94686 18-19 CGTAGCG 805 0.0 15.045948 56-57 >>END_MODULE