FastQCFastQC Report
Sat 18 Jun 2016
SRR578606_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR578606_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13598736
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT223269216.41837888462575No Hit
AAGCAGTGGTATCAACGCAGAGTATTTTTTTTTTTTTTTTTTTTTTTTTT522630.38432248408969777No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA348700.2564208908827997No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAG259610.1909074490452642No Hit
AAGCAGTGGTATCAACGCAGAGTACCTTTTTTTTTTTTTTTTTTTTTTTT147240.1082747690667721No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT4700700.092.33371
AGTGGTA4689250.091.775315
AGCAGTG4734950.091.43962
GGTATCA4724200.091.379338
TGGTATC4715550.091.3390357
GTGGTAT4728200.091.280276
GTATCAA4736800.091.248949
GCAGTGG4743850.091.0763553
CAGTGGT4752350.090.6630864
GTATCGA9750.047.762689
ATCAACG4737150.046.0633410-11
TCAACGC4744650.046.03880312-13
CAACGCA4746300.046.02680612-13
AACGCAG4768200.045.8890514-15
ACGCAGA4769350.045.88246514-15
CGCAGAG4773400.045.80693416-17
TATCAAC4755800.045.7852910-11
AGAGTAC4648350.045.69842518-19
CAGAGTA4731350.045.64675518-19
GCAGAGT4776350.045.4961816-17
Produced by FastQC (version 0.11.5)