##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR578606_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 13598736 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.55276424220604 31.0 31.0 33.0 27.0 34.0 2 30.844454587544018 31.0 31.0 34.0 27.0 34.0 3 30.96737005556987 31.0 31.0 34.0 27.0 34.0 4 34.39801897764616 37.0 35.0 37.0 30.0 37.0 5 34.43498167770887 37.0 35.0 37.0 30.0 37.0 6 34.4462216194211 37.0 35.0 37.0 30.0 37.0 7 34.31224541751528 37.0 35.0 37.0 30.0 37.0 8 34.29606788454456 37.0 35.0 37.0 30.0 37.0 9 35.719029548040346 39.0 35.0 39.0 30.0 39.0 10-11 35.65554901573205 39.0 35.0 39.0 30.0 39.0 12-13 35.767353708462316 39.0 35.0 39.0 30.0 39.0 14-15 36.80401538790076 40.0 36.0 41.0 30.0 41.0 16-17 36.760097519357686 40.0 36.0 41.0 30.0 41.0 18-19 36.699728048253895 40.0 36.0 41.0 30.0 41.0 20-21 36.53943160599632 39.5 36.0 41.0 29.5 41.0 22-23 36.19543643615113 39.0 35.5 40.5 28.5 41.0 24-25 36.106866880863045 39.0 35.5 40.0 28.5 41.0 26-27 36.07630253282364 39.0 35.5 40.0 28.0 41.0 28-29 35.79784099051559 39.0 35.0 41.0 27.0 41.0 30-31 35.486131615467784 38.5 34.5 40.5 25.5 41.0 32-33 35.10821722695404 38.0 34.0 40.0 25.0 41.0 34-35 34.902153626631176 38.0 33.5 40.0 24.5 41.0 36-37 34.84355204042494 38.0 34.0 40.0 24.5 41.0 38-39 33.94257642769152 36.0 33.0 40.0 24.0 41.0 40-41 33.875302233972334 36.0 33.0 40.0 24.0 41.0 42-43 33.65633544911822 36.0 33.0 40.0 22.5 41.0 44-45 33.48352464523174 35.5 33.0 40.0 22.0 41.0 46-47 33.27956024736417 35.0 33.0 40.0 21.5 41.0 48-49 32.916686153771934 35.0 32.5 40.0 19.5 41.0 50-51 31.60536100561111 34.5 30.5 38.5 13.5 40.0 52-53 31.144240244093275 35.0 30.0 38.5 6.5 40.0 54-55 30.577078671135318 35.0 28.5 39.0 2.0 41.0 56-57 29.94359681664531 35.0 27.0 39.0 2.0 41.0 58-59 29.667310402966862 35.0 27.0 39.0 2.0 41.0 60-61 29.41526260234775 35.0 27.0 39.0 2.0 41.0 62-63 29.0907563026446 35.0 26.0 38.5 2.0 40.5 64-65 28.759550372917012 35.0 26.0 38.0 2.0 40.0 66-67 28.458894341356434 35.0 26.0 37.5 2.0 40.0 68-69 28.122442740266447 34.5 26.0 37.0 2.0 40.0 70-71 27.73653948425795 34.0 25.0 36.5 2.0 39.0 72-73 27.357746484673278 34.0 24.0 36.0 2.0 39.0 74-75 26.968338086716294 34.0 23.5 35.5 2.0 38.5 76-77 26.573395387630143 34.0 21.5 35.0 2.0 37.5 78-79 26.201244659797794 33.0 20.0 35.0 2.0 37.0 80-81 25.832352065662576 33.0 19.5 35.0 2.0 36.5 82-83 25.478173265515267 33.0 18.0 35.0 2.0 36.0 84-85 25.08135116381405 33.0 14.5 35.0 2.0 36.0 86-87 24.72750066623839 32.0 8.5 35.0 2.0 35.0 88-89 24.33933804583014 32.0 4.5 35.0 2.0 35.0 90-91 23.94945328006956 32.0 2.0 35.0 2.0 35.0 92-93 23.552924073237392 31.5 2.0 35.0 2.0 35.0 94-95 23.103198819360856 31.0 2.0 35.0 2.0 35.0 96-97 22.606866917631166 31.0 2.0 34.5 2.0 35.0 98-99 21.991818357235555 31.0 2.0 34.0 2.0 35.0 100-101 20.475938351917414 28.5 2.0 33.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.567629396465275 1101 2 0.5980527143717147 1101 3 0.6128917168871055 1101 4 0.702932885956848 1101 5 0.6622039738185634 1101 6 0.5418152646522358 1101 7 0.5854049225499764 1101 8 0.6780350848243089 1101 9 0.6141827518475438 1101 10-11 0.6812335040190618 1101 12-13 0.6369412535778665 1101 14-15 0.8268073865652212 1101 16-17 0.7688185342343132 1101 18-19 0.7212330646814848 1101 20-21 0.7722861414409792 1101 22-23 0.8487399240617748 1101 24-25 0.9637130145862969 1101 26-27 0.8882703499727995 1101 28-29 0.8360975774574655 1101 30-31 1.020641121640054 1101 32-33 1.122640858094087 1101 34-35 1.016490156267004 1101 36-37 1.0142508711320062 1101 38-39 1.125143308679121 1101 40-41 1.041360930154866 1101 42-43 1.2771097014704864 1101 44-45 1.1559331145847622 1101 46-47 1.1393205296720197 1101 48-49 1.197067858672746 1101 50-51 1.209799713842063 1101 52-53 1.2357875950700254 1101 54-55 1.086743446558721 1101 56-57 1.0562104294011228 1101 58-59 1.1472844490134975 1101 60-61 1.2519350081659617 1101 62-63 1.2568692539204633 1101 64-65 1.2385676236222736 1101 66-67 1.143801790881664 1101 68-69 1.2564417115800026 1101 70-71 1.195663112504139 1101 72-73 1.2688550096123024 1101 74-75 1.33742824516197 1101 76-77 1.1971358817578093 1101 78-79 1.2811009506136344 1101 80-81 1.4018542205935631 1101 82-83 1.5248688225911025 1101 84-85 1.601614106480266 1101 86-87 1.5443184008920916 1101 88-89 1.6734180724318293 1101 90-91 1.826517754140493 1101 92-93 1.8177213108404366 1101 94-95 1.840359800530102 1101 96-97 1.989900509471525 1101 98-99 2.2723033548860236 1101 100-101 2.563039058391549 1102 1 0.37822496125782834 1102 2 0.3519616324643309 1102 3 0.3845749211431446 1102 4 0.49050275931409004 1102 5 0.46016443537878615 1102 6 0.38281513126101885 1102 7 0.45583893219259863 1102 8 0.5069261881461316 1102 9 0.4246298161623798 1102 10-11 0.5585832793634822 1102 12-13 0.557233482895306 1102 14-15 0.7189960170062051 1102 16-17 0.7130858541712044 1102 18-19 0.657207891474421 1102 20-21 0.6424627906151343 1102 22-23 0.6528973758145042 1102 24-25 0.8224799708493649 1102 26-27 0.9041601199395117 1102 28-29 0.7331656656313683 1102 30-31 0.8282183960328169 1102 32-33 0.8616238666357745 1102 34-35 0.9494868447472768 1102 36-37 0.7638099144137556 1102 38-39 0.8359406065161323 1102 40-41 0.8697783201360778 1102 42-43 1.0218713448453016 1102 44-45 0.9708177249272794 1102 46-47 0.9525227343095182 1102 48-49 1.0637096632761924 1102 50-51 0.8473187421000539 1102 52-53 0.9118809412060962 1102 54-55 0.867954001829883 1102 56-57 0.7659866358160166 1102 58-59 0.7894112895221319 1102 60-61 0.8626497371452579 1102 62-63 0.8359908245948517 1102 64-65 0.7850299379652697 1102 66-67 0.7481451052729433 1102 68-69 0.9780522902119628 1102 70-71 0.8671437236589803 1102 72-73 0.9140638148470615 1102 74-75 1.0240551006945964 1102 76-77 0.8595732151417153 1102 78-79 0.8628834618071899 1102 80-81 0.9668388970882518 1102 82-83 1.080891537320582 1102 84-85 1.1151210673634182 1102 86-87 1.1331538122145979 1102 88-89 1.2559495264917295 1102 90-91 1.264709014797564 1102 92-93 1.2144285219347282 1102 94-95 1.2019411915762106 1102 96-97 1.4541080658675156 1102 98-99 1.5298371563380648 1102 100-101 1.7995839623973389 1103 1 0.35202896192398114 1103 2 0.3155603593262306 1103 3 0.20249565071623365 1103 4 0.3911494922468819 1103 5 0.30569078816961337 1103 6 0.20509498409665383 1103 7 0.4730826334527123 1103 8 0.34764248225327776 1103 9 0.601053048489355 1103 10-11 0.5169583741440533 1103 12-13 0.390980929906398 1103 14-15 0.6095701314408046 1103 16-17 0.5962423416959766 1103 18-19 0.577660251250343 1103 20-21 0.7350722096865496 1103 22-23 0.695324889188754 1103 24-25 0.5815024998242464 1103 26-27 0.7378638097748009 1103 28-29 0.6716876771281761 1103 30-31 0.5431069810123077 1103 32-33 0.8138278158496206 1103 34-35 0.8217365212848833 1103 36-37 0.7907443388306419 1103 38-39 0.5443969638356165 1103 40-41 0.6586190737425852 1103 42-43 0.45493493396179474 1103 44-45 0.6290735203466227 1103 46-47 0.7387755902963349 1103 48-49 0.7306270750442607 1103 50-51 0.7531004129672247 1103 52-53 0.6787516452073952 1103 54-55 0.723103156849433 1103 56-57 0.6341477095470935 1103 58-59 0.7079591012686066 1103 60-61 0.7641272940859309 1103 62-63 0.663768121266056 1103 64-65 0.6849934425951822 1103 66-67 0.6355398035770037 1103 68-69 0.7298847328990519 1103 70-71 0.7580585453462554 1103 72-73 0.7921475021631004 1103 74-75 0.986973521176651 1103 76-77 0.9970372953353355 1103 78-79 1.055066979170956 1103 80-81 0.9426544047063352 1103 82-83 1.0917539350775307 1103 84-85 1.0630072026908373 1103 86-87 1.1826750159816157 1103 88-89 1.2990806223950209 1103 90-91 1.0531695490335942 1103 92-93 1.1412260470901714 1103 94-95 1.2550933147403143 1103 96-97 1.450440589354038 1103 98-99 1.5238780210967349 1103 100-101 1.7480809395190988 1104 1 0.18778005568349343 1104 2 0.20380297973778028 1104 3 0.21992936104972216 1104 4 0.15273520949109098 1104 5 0.06566534749362773 1104 6 0.1010818815489003 1104 7 0.2810432961507914 1104 8 0.2400466306476403 1104 9 0.35757664633143804 1104 10-11 0.4220648316192168 1104 12-13 0.2667682945855674 1104 14-15 0.3417237789907901 1104 16-17 0.5031030520284361 1104 18-19 0.6335746845560237 1104 20-21 0.4642900133498813 1104 22-23 0.4039343703635012 1104 24-25 0.4331003484243112 1104 26-27 0.49624226616833056 1104 28-29 0.6419847626884874 1104 30-31 0.5558565908076005 1104 32-33 0.7173584317669821 1104 34-35 0.6558438003691833 1104 36-37 0.7664602938572926 1104 38-39 0.5503709820181726 1104 40-41 0.562120611285799 1104 42-43 0.7379008401161471 1104 44-45 0.9707346002258816 1104 46-47 0.727881894836024 1104 48-49 0.6637073717184734 1104 50-51 0.6641375047868046 1104 52-53 0.4964989780563904 1104 54-55 0.6268796494404469 1104 56-57 0.7146112217324152 1104 58-59 0.7655543983864028 1104 60-61 0.638035041686674 1104 62-63 0.5989013688858869 1104 64-65 0.7414144908118985 1104 66-67 0.5262455589287143 1104 68-69 0.6465676957055528 1104 70-71 0.8102438141774364 1104 72-73 0.5957868992465478 1104 74-75 0.7054701734240574 1104 76-77 0.7752363468645349 1104 78-79 0.8240499914042481 1104 80-81 0.7188945586785849 1104 82-83 0.7951682358864147 1104 84-85 0.7902592260556673 1104 86-87 0.9190009750134749 1104 88-89 1.0460072848656345 1104 90-91 1.1554702351542758 1104 92-93 1.0996474698440828 1104 94-95 1.1923111142772278 1104 96-97 1.3520408380482856 1104 98-99 1.5227320365687511 1104 100-101 1.8260767228969463 1105 1 -0.05483127772754415 1105 2 0.11903727877940184 1105 3 0.11318092017213033 1105 4 0.10359060313350454 1105 5 0.14362619975399582 1105 6 0.16982319594982442 1105 7 0.3313986780377789 1105 8 0.28814305611480506 1105 9 0.17619677602745298 1105 10-11 0.27134301659272353 1105 12-13 0.18744426332579422 1105 14-15 0.1097206677356155 1105 16-17 0.3416557003868448 1105 18-19 0.12991814579434902 1105 20-21 0.13649305015555768 1105 22-23 0.33047924273223117 1105 24-25 0.5395299796070745 1105 26-27 0.46624520296654026 1105 28-29 0.5409715279395542 1105 30-31 0.43989980697450193 1105 32-33 0.5503804508290671 1105 34-35 0.4293486287854904 1105 36-37 0.739868275143877 1105 38-39 0.5067639737454996 1105 40-41 0.512501428599407 1105 42-43 0.7610091319468637 1105 44-45 0.8090237225266961 1105 46-47 0.47937221316554 1105 48-49 0.6629539082761582 1105 50-51 0.670224242370768 1105 52-53 0.40324390820793354 1105 54-55 0.5396428285576924 1105 56-57 0.6212941184548484 1105 58-59 0.5769013444622324 1105 60-61 0.4988984140575816 1105 62-63 0.5558762661934971 1105 64-65 0.7317476286802282 1105 66-67 0.5090896762629669 1105 68-69 0.6212751461946553 1105 70-71 0.6101858332305561 1105 72-73 0.484018010618783 1105 74-75 0.48438676023643623 1105 76-77 0.40609747415403064 1105 78-79 0.5279756239545144 1105 80-81 0.5762546352452631 1105 82-83 0.7512081188606139 1105 84-85 0.8378950049499245 1105 86-87 0.8432726092474816 1105 88-89 0.7177235962385353 1105 90-91 0.9739293657588668 1105 92-93 1.0930781260514415 1105 94-95 1.1712773028046968 1105 96-97 1.335927072149392 1105 98-99 1.5492464522288394 1105 100-101 1.8594832000931767 1106 1 0.2133923174960124 1106 2 0.32465131676922 1106 3 0.21487915773490585 1106 4 0.13124186959827 1106 5 0.27771257547787087 1106 6 0.23716498151803478 1106 7 0.3090318682990798 1106 8 0.26465268754852644 1106 9 0.3360085268827504 1106 10-11 0.1745322490470329 1106 12-13 0.34731233049051013 1106 14-15 0.22494967209742356 1106 16-17 0.3253932764594012 1106 18-19 0.38263941625089615 1106 20-21 0.34314190227426167 1106 22-23 0.47003743431464073 1106 24-25 0.599980124828484 1106 26-27 0.48072654004372595 1106 28-29 0.4869552425190662 1106 30-31 0.5634791228683227 1106 32-33 0.4769287473192705 1106 34-35 0.4082425565684389 1106 36-37 0.5230912610039198 1106 38-39 0.5078035556120071 1106 40-41 0.4069653254996979 1106 42-43 0.6058170467685784 1106 44-45 0.5984258984464788 1106 46-47 0.7080077282741613 1106 48-49 0.7592524264648617 1106 50-51 0.6361132303992285 1106 52-53 0.6377326219080324 1106 54-55 0.783800708478271 1106 56-57 0.5856175835611417 1106 58-59 0.7028082646773903 1106 60-61 0.5936615730380161 1106 62-63 0.6584082281866053 1106 64-65 0.7803623627752891 1106 66-67 0.7551822574668279 1106 68-69 0.7536062482216046 1106 70-71 0.8459909451977943 1106 72-73 0.7812603682839026 1106 74-75 0.7451575472135623 1106 76-77 0.8370138733830075 1106 78-79 0.9129053230674131 1106 80-81 0.9421837132100492 1106 82-83 1.134454469744366 1106 84-85 1.1874418954913075 1106 86-87 1.1959815307453816 1106 88-89 1.2449529827034134 1106 90-91 1.1304467083863052 1106 92-93 1.2871923005877157 1106 94-95 1.3452638822140983 1106 96-97 1.4968292460558459 1106 98-99 1.575182481036837 1106 100-101 1.7576086495204635 1107 1 0.2087662630823246 1107 2 0.24508582854485184 1107 3 0.24425372360806108 1107 4 0.14945934477817246 1107 5 0.24536201719917017 1107 6 0.3171138098048587 1107 7 0.35364632504546023 1107 8 0.3623979639773296 1107 9 0.30752298643909626 1107 10-11 0.29399482866878657 1107 12-13 0.3594216887370365 1107 14-15 0.41278213294569355 1107 16-17 0.3170998181953095 1107 18-19 0.30427723068526547 1107 20-21 0.36570818174539 1107 22-23 0.51421736367449 1107 24-25 0.5665435895719142 1107 26-27 0.5013985547381807 1107 28-29 0.6310995045638279 1107 30-31 0.6565005430996536 1107 32-33 0.6257920462091988 1107 34-35 0.5664988783467422 1107 36-37 0.48928669104399347 1107 38-39 0.6300478258360585 1107 40-41 0.6033715450224832 1107 42-43 0.5827899709746944 1107 44-45 0.5714395771085563 1107 46-47 0.6818198249480432 1107 48-49 0.7050114739887192 1107 50-51 0.6184675712404193 1107 52-53 0.7042211122163735 1107 54-55 0.8317347361105547 1107 56-57 0.6184880182351904 1107 58-59 0.6550095614790798 1107 60-61 0.6475539710589793 1107 62-63 0.7019885910940538 1107 64-65 0.7034895419338802 1107 66-67 0.7508194470132423 1107 68-69 0.7537665963143034 1107 70-71 0.7671352497411732 1107 72-73 0.7781928184551319 1107 74-75 0.8616555036849469 1107 76-77 0.9216815562166474 1107 78-79 0.9005544426084633 1107 80-81 0.9561487454687452 1107 82-83 0.941240338070358 1107 84-85 1.0543188091618703 1107 86-87 1.04560641362254 1107 88-89 1.1328762029702766 1107 90-91 1.1742592834521446 1107 92-93 1.1457735067919472 1107 94-95 1.1909692811293198 1107 96-97 1.397000900241185 1107 98-99 1.5144825540506694 1107 100-101 1.7381573200180434 1108 1 0.2148486870902495 1108 2 0.17240987271804187 1108 3 0.0028864311592649017 1108 4 0.22345076075631454 1108 5 0.3023010383202731 1108 6 0.29498350245788885 1108 7 0.2698224838763821 1108 8 0.17121414714351602 1108 9 0.18247568584963147 1108 10-11 0.2412984155834934 1108 12-13 0.2726247546236209 1108 14-15 0.2070730720692211 1108 16-17 0.319819077130127 1108 18-19 0.12892097187284435 1108 20-21 0.13889992546003072 1108 22-23 0.2982314857100903 1108 24-25 0.350886107139182 1108 26-27 0.3257724888482869 1108 28-29 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0.1263125984359803 2311 12-13 0.09322609566294915 2311 14-15 0.318409681026381 2311 16-17 0.2964926164820483 2311 18-19 0.02574551729911434 2311 20-21 0.08224624540419967 2311 22-23 0.2068410815364885 2311 24-25 0.12635750782762756 2311 26-27 0.22361592845202694 2311 28-29 0.21417088755890035 2311 30-31 0.08885760859174496 2311 32-33 0.1434324780622802 2311 34-35 0.10810405472474827 2311 36-37 0.2453186675803991 2311 38-39 0.07186375800942102 2311 40-41 0.13326326461890403 2311 42-43 0.21272277793729444 2311 44-45 0.14449170706061665 2311 46-47 0.08616642005087982 2311 48-49 0.08538025875220256 2311 50-51 0.22184728573048673 2311 52-53 0.26086596503130366 2311 54-55 -0.07267375761213657 2311 56-57 -1.145341402875033E-5 2311 58-59 0.0743687115199414 2311 60-61 0.23480903394568386 2311 62-63 0.06955161040668756 2311 64-65 0.16620385749945044 2311 66-67 0.1191646188475346 2311 68-69 0.06867433882673168 2311 70-71 0.14229678900951015 2311 72-73 0.14522527917671368 2311 74-75 0.08365289112397534 2311 76-77 0.27761385871702515 2311 78-79 0.25075207757049967 2311 80-81 0.15922041004776588 2311 82-83 0.17161987926497702 2311 84-85 0.17396265288304846 2311 86-87 0.2106393048647064 2311 88-89 0.14789905161633143 2311 90-91 0.14850200417821569 2311 92-93 0.24774624914712362 2311 94-95 0.23574092483313436 2311 96-97 0.2619705493676605 2311 98-99 0.17762000167831005 2311 100-101 0.14271441886382163 2312 1 -0.5671261428339172 2312 2 -0.11420609595604247 2312 3 -0.24873583855104542 2312 4 -0.1303076732168833 2312 5 -0.10353348366370341 2312 6 -0.05415844068086528 2312 7 -0.04400988225513203 2312 8 -0.0972280594046282 2312 9 -0.1983895127654023 2312 10-11 -0.2731882153848346 2312 12-13 -0.23301743945571474 2312 14-15 -0.07632331797876901 2312 16-17 -0.017568387521784246 2312 18-19 -0.2562058130875897 2312 20-21 -0.3316879522385676 2312 22-23 -0.23392539693929848 2312 24-25 -0.07809692821162884 2312 26-27 -0.12798454528090275 2312 28-29 -0.15145187744636956 2312 30-31 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0.005600521548860371 2312 94-95 -0.05598983319372053 2312 96-97 0.09122885752024956 2312 98-99 0.018653790138849047 2312 100-101 0.006461067926359476 2313 1 -0.5037819435367474 2313 2 -0.3480267062414448 2313 3 -0.34703223456433463 2313 4 -1.2485395256658762 2313 5 -1.0548585746916999 2313 6 -0.5667125291791208 2313 7 -0.38113500215467155 2313 8 -0.3423905009984409 2313 9 -0.2948246374391843 2313 10-11 -0.5238871155701474 2313 12-13 -0.9365738141979776 2313 14-15 -1.163628391544691 2313 16-17 -0.776121044617355 2313 18-19 -0.645918584660599 2313 20-21 -0.6813109350347801 2313 22-23 -0.8324522218255268 2313 24-25 -0.6160202442481193 2313 26-27 -0.6274410514235029 2313 28-29 -0.5266028899941873 2313 30-31 -0.8779440147015336 2313 32-33 -0.8145236131886904 2313 34-35 -0.7689606892023022 2313 36-37 -0.9659440748405146 2313 38-39 -0.6745184640357635 2313 40-41 -0.5701104717483219 2313 42-43 -0.5539058307585023 2313 44-45 -0.4110614238668191 2313 46-47 -0.6133314887932997 2313 48-49 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70-71 -2.4171894277936765 2314 72-73 -1.5938920608431957 2314 74-75 -1.7730373456482909 2314 76-77 -1.5116628116007327 2314 78-79 -1.836847170198947 2314 80-81 -2.602248664133448 2314 82-83 -1.5387625177169966 2314 84-85 -1.7992275649715594 2314 86-87 -1.241430990970482 2314 88-89 -1.2438865884685448 2314 90-91 -1.8223336598832525 2314 92-93 -1.2778867717145523 2314 94-95 -1.1991327365055184 2314 96-97 -1.3458097403863754 2314 98-99 -1.2394476655981137 2314 100-101 -1.2529228800436059 2315 1 -0.7453903505930342 2315 2 -0.6446903793354544 2315 3 -0.7841555890859482 2315 4 -0.6650111576011 2315 5 -0.5960853740212713 2315 6 -0.696015461017673 2315 7 -1.3211304404115722 2315 8 -0.7489784585228279 2315 9 -3.173011885549286 2315 10-11 -1.252388780889639 2315 12-13 -0.9405144716537421 2315 14-15 -1.1361256508857736 2315 16-17 -1.5216861091684137 2315 18-19 -0.7902860640503206 2315 20-21 -1.1911418167710153 2315 22-23 -1.2246576877229813 2315 24-25 -2.538281695724656 2315 26-27 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92-93 -1.859477739563797 2315 94-95 -2.0144622513557735 2315 96-97 -1.921986063260153 2315 98-99 -2.125789416265416 2315 100-101 -2.161322910036528 2316 1 -1.338737375689412 2316 2 -1.2311164816241131 2316 3 -2.449890170662748 2316 4 -1.5751319601401406 2316 5 -1.3289341064089086 2316 6 -2.9213057081299034 2316 7 -7.09299450618359 2316 8 -7.810903644457905 2316 9 -4.538233135766653 2316 10-11 -2.4275407201447194 2316 12-13 -2.2840473481090484 2316 14-15 -2.33680397457519 2316 16-17 -2.340927876711646 2316 18-19 -1.8193743345247242 2316 20-21 -2.922176408847683 2316 22-23 -3.5390842123141084 2316 24-25 -2.3016511386487153 2316 26-27 -2.55916802627727 2316 28-29 -3.3558341799291824 2316 30-31 -3.8374769009077383 2316 32-33 -3.8029787348731716 2316 34-35 -2.9911929107624573 2316 36-37 -4.036348774381658 2316 38-39 -3.534035366314832 2316 40-41 -4.307606226893789 2316 42-43 -3.6357251766986636 2316 44-45 -3.331453635189316 2316 46-47 -2.9878369972503975 2316 48-49 -2.990845459327584 2316 50-51 -2.7694642737260615 2316 52-53 -3.1689113710862706 2316 54-55 -4.60063730028476 2316 56-57 -3.2802366716066054 2316 58-59 -2.7487469885870333 2316 60-61 -3.425967058754569 2316 62-63 -3.1618014417261193 2316 64-65 -4.570440778475881 2316 66-67 -3.5382667919560866 2316 68-69 -3.4495659236767793 2316 70-71 -3.837751000260017 2316 72-73 -3.453844599126448 2316 74-75 -3.261550963887565 2316 76-77 -3.771540076993574 2316 78-79 -4.806926291013184 2316 80-81 -3.797094251478427 2316 82-83 -3.4160666042360397 2316 84-85 -3.5411046855436297 2316 86-87 -4.575160521820994 2316 88-89 -4.9900308850136135 2316 90-91 -4.4497866963619295 2316 92-93 -4.539580954327956 2316 94-95 -4.671702470400341 2316 96-97 -4.8532373055642 2316 98-99 -4.958619791469307 2316 100-101 -4.924980839072095 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 139096.0 3 26278.0 4 23778.0 5 23435.0 6 26535.0 7 33693.0 8 42600.0 9 50627.0 10 59464.0 11 71178.0 12 72991.0 13 81405.0 14 90620.0 15 106489.0 16 131934.0 17 170083.0 18 236497.0 19 343915.0 20 479159.0 21 357504.0 22 209256.0 23 192586.0 24 205168.0 25 216981.0 26 233775.0 27 262424.0 28 298637.0 29 347378.0 30 406455.0 31 481353.0 32 581198.0 33 715727.0 34 922559.0 35 1304219.0 36 1846022.0 37 1621404.0 38 1047768.0 39 138467.0 40 78.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 10.401273709494841 52.865135883820024 20.226705565116557 16.506884841568578 2 15.644887376077907 53.825741578023944 16.197036227687363 14.33233481821079 3 50.18223560599302 18.0152881804795 16.552353501152627 15.250122712374855 4 15.306464783293983 16.940358720035942 17.54506081688514 50.20811567978494 5 15.503646815381517 51.60619899728771 18.3551761459527 14.534978041378077 6 50.077232680239334 17.70847064425826 16.74675504614199 15.467541629360415 7 16.115027909612223 17.300868078225527 52.09022878712688 14.49387522503537 8 49.67819879947149 17.90764190354274 17.455756337155183 14.958402959830586 9 49.30005663549813 17.67938714429393 18.015726194782687 15.004830025425253 10-11 15.030797631272991 34.91333345795257 34.7261948690534 15.329674041721047 12-13 14.862801327668556 18.11982425795124 34.57862050365602 32.43875391072419 14-15 15.139076843663856 52.18396303809786 17.256413831356852 15.420546286881438 16-17 32.3494950505093 17.8431491802617 17.384704588570447 32.422651180658555 18-19 15.315150950582204 34.95196023548373 17.410183711923878 32.32270510201018 20-21 32.46291342434818 34.962201738522104 17.512551901192623 15.062332935937093 22-23 32.3565330437508 17.827980176545285 34.63857730808903 15.17690947161488 24-25 15.142983274809568 34.65725901604223 18.121756284596 32.0780014245522 26-27 15.370473523054681 24.4472397234719 44.808535750227904 15.373751003245514 28-29 29.15570853583383 17.59394264016957 38.045326832570694 15.205021991425907 30-31 25.97138860936615 19.41042211975085 38.5800803274657 16.0381089434173 32-33 19.15811704298254 22.00433012942014 40.524938525294985 18.312614302302332 34-35 18.543061877765528 20.9923103842262 41.30615966875773 19.158468069250542 36-37 18.74377704156629 20.80410279516182 41.415236399398545 19.036883763873337 38-39 18.940643072468387 20.789325130195806 41.26872602255188 19.001305774783926 40-41 18.8597916104352 20.79772119599172 40.946493420840376 19.3959937727327 42-43 19.28807544749154 20.923908275293307 41.004876462137624 18.78313981507753 44-45 18.69736010986615 21.054440475698684 40.873055972533614 19.375143441901553 46-47 19.178496712616212 20.89408466618499 40.6940080265938 19.233410594605 48-49 19.00529132760374 21.126218259373122 40.77129003230593 19.097200380717204 50-51 19.222141304790462 20.806660101797046 40.493319456098014 19.477879137314478 52-53 19.602939823535102 21.794296296211826 39.16863838034774 19.434125499905335 54-55 20.155356395592133 22.60847660432017 37.55681105886632 19.679355941221374 56-57 20.499861632250933 23.41963633137235 36.31725784439196 19.763244191984764 58-59 20.620772784343153 24.269727061577907 35.33173433589916 19.777765818179784 60-61 20.521479904155367 24.527877197510843 34.875143860197404 20.075499038136382 62-63 20.39829711579257 24.64991954661957 34.74077729160066 20.211006045987205 64-65 20.18441821640942 24.265204932876998 34.99016001118895 20.56021683952464 66-67 20.21436628029542 23.736253574031206 35.830456261737254 20.218923883936117 68-69 20.080526062311307 23.619990023534555 36.14784399356418 20.151639920589954 70-71 20.077407887854353 23.565254488216564 36.48683978722175 19.870497836707337 72-73 19.696098294637824 23.37306512190436 36.556377071799176 20.374459511658642 74-75 20.278987070884057 23.49422715098163 35.984984868774674 20.241800909359643 76-77 20.623676277783336 24.016171779992316 35.17853207682464 20.18161986539971 78-79 20.386942318963442 24.09652634537641 34.999943166826114 20.516588168834033 80-81 20.268339554512536 24.783013453101532 34.49029695056582 20.45835004182011 82-83 21.22815869144869 24.806808000258062 33.70018905676238 20.264844251530864 84-85 20.98318010577945 25.31977928287055 33.32530541437213 20.371735196977873 86-87 20.734412768641793 25.556534168621397 33.22659371482713 20.482459347909675 88-89 20.55131907333688 25.143523398362156 33.199241074946165 21.105916453354794 90-91 20.548009753437288 25.286792938237145 33.169109740441584 20.996087567883986 92-93 20.82057474020545 25.41107875323335 33.25317981346605 20.51516669309515 94-95 20.670487830528277 25.267456763613428 32.642090158804244 21.41996524705406 96-97 20.617314114545575 25.00938527039785 33.070824062233385 21.302476552823194 98-99 20.99206021104703 25.42156957363651 32.44403396242959 21.142336252886867 100-101 21.60099642419876 24.891605916097667 32.15883929099771 21.34855836870586 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 41489.0 1 24613.0 2 5417.0 3 2673.5 4 2032.5 5 1722.0 6 2075.0 7 2681.5 8 2399.5 9 2391.0 10 3907.5 11 27836.5 12 494914.5 13 535905.0 14 111125.5 15 89171.0 16 91561.0 17 96921.0 18 100886.5 19 102297.5 20 102200.0 21 102129.0 22 102505.0 23 103708.5 24 106895.0 25 111176.5 26 117093.0 27 125717.5 28 132147.0 29 137810.0 30 146428.5 31 156877.5 32 167478.0 33 179524.5 34 201623.5 35 217103.5 36 226211.5 37 241086.5 38 254012.0 39 268160.5 40 285267.0 41 303704.0 42 319928.0 43 335749.0 44 352847.5 45 366754.0 46 375172.0 47 395151.0 48 411214.0 49 415612.5 50 420105.5 51 418612.0 52 422651.5 53 429748.0 54 414693.0 55 387453.5 56 370185.0 57 352534.0 58 354642.0 59 316093.0 60 241220.5 61 197357.0 62 158883.5 63 123765.5 64 98098.5 65 80483.5 66 64247.5 67 51154.0 68 40066.0 69 31178.5 70 25268.0 71 21412.0 72 18289.5 73 14684.5 74 11705.0 75 9160.0 76 7177.0 77 5763.0 78 4652.0 79 3829.0 80 3268.5 81 2909.0 82 2594.0 83 2265.0 84 2033.0 85 1796.0 86 1431.5 87 1139.5 88 920.0 89 723.0 90 567.0 91 421.0 92 301.0 93 219.5 94 158.0 95 108.0 96 72.0 97 47.5 98 41.5 99 32.0 100 11.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.12031265258771108 2 0.10956165337719624 3 0.10386259428817501 4 0.09222916012194075 5 0.09695018713503961 6 0.08729487799454302 7 0.09628836091825005 8 0.0923100499928817 9 0.08714780550192312 10-11 0.07852200380976584 12-13 0.06986678761908459 14-15 0.07781973265750582 16-17 0.06844386125298704 18-19 0.06124098592692733 20-21 0.05711560250893907 22-23 0.04968108800700301 24-25 0.047022752702898275 26-27 0.043948937607142316 28-29 0.04617708587033383 30-31 0.03771674073237395 32-33 0.04973624019173546 34-35 0.060299721974159955 36-37 0.05095326506816516 38-39 0.05286153065990839 40-41 0.05309684664810023 42-43 0.04858907474930023 44-45 0.04824345439164346 46-47 0.048125796397547535 48-49 0.04776179197831328 50-51 0.04920677921830381 52-53 0.046235914867381794 54-55 0.046416078670841174 56-57 0.046581535225038564 58-59 0.04910382847346989 60-61 0.04494903055695765 62-63 0.05386162360972373 64-65 0.04962961263458603 66-67 0.04461076382393187 68-69 0.042250250317382436 70-71 0.050295115663691096 72-73 0.04450045945446694 74-75 0.04643078592010316 76-77 0.04172814296858179 78-79 0.04649329172946662 80-81 0.048471416755204305 82-83 0.03823517126885911 84-85 0.04844200225668033 86-87 0.051019447689844115 88-89 0.04671757728071198 90-91 0.04253704167799125 92-93 0.0400515165527149 94-95 0.04361802449874753 96-97 0.03158749460243952 98-99 0.032503020868998415 100-101 0.02442506421185028 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.3598736E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 38.62135540376397 #Duplication Level Percentage of deduplicated Percentage of total 1 88.78853292828506 34.291334860020974 2 5.790787075726119 4.4729609143828295 3 1.8737307784591175 2.1709806697752274 4 0.9753935260983886 1.5068408011990557 5 0.6010107949905307 1.1605925757414008 6 0.39578866049464967 0.9171536713046125 7 0.266541462842525 0.7205934796396213 8 0.19075486792658275 0.5893769239352488 9 0.14272067857017567 0.4960859445472604 >10 0.9388132983727201 6.587529868082571 >50 0.017037370265609447 0.4162400560488677 >100 0.00985517538022145 0.7993734103689171 >500 0.003664485400810651 1.042234051534384 >1k 0.004388860399296684 3.567362489850519 >5k 5.539338368044358E-4 1.49716233053305 >10k+ 4.261029513880276E-4 39.76417795303548 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 2232692 16.41837888462575 No Hit AAGCAGTGGTATCAACGCAGAGTATTTTTTTTTTTTTTTTTTTTTTTTTT 52263 0.38432248408969777 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 34870 0.2564208908827997 No Hit AAGCAGTGGTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAG 25961 0.1909074490452642 No Hit AAGCAGTGGTATCAACGCAGAGTACCTTTTTTTTTTTTTTTTTTTTTTTT 14724 0.1082747690667721 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 7.353624630995116E-6 0.0 0.0 0.0 0.0 2 2.9414498523980465E-5 0.0 0.0 0.0 0.0 3 5.882899704796093E-5 1.4707249261990233E-5 0.0 0.0 0.0 4 6.618262167895605E-5 1.4707249261990233E-5 0.0 0.0 0.0 5 6.618262167895605E-5 2.206087389298535E-5 0.0 0.0 0.0 6 9.559712020293651E-5 2.206087389298535E-5 0.0 7.353624630995116E-6 0.0 7 9.559712020293651E-5 2.9414498523980465E-5 0.0 7.353624630995116E-6 7.353624630995116E-6 8 9.559712020293651E-5 2.9414498523980465E-5 0.0 1.4707249261990233E-5 7.353624630995116E-6 9 1.1765799409592186E-4 2.9414498523980465E-5 0.0 2.206087389298535E-5 7.353624630995116E-6 10-11 1.2501161872691696E-4 2.9414498523980465E-5 0.0 2.206087389298535E-5 1.1030436946492674E-5 12-13 1.3604205567340963E-4 2.9414498523980465E-5 0.0 2.206087389298535E-5 1.838406157748779E-5 14-15 1.507493049353999E-4 2.9414498523980465E-5 0.0 2.5737686208482907E-5 2.206087389298535E-5 16-17 1.91194240405873E-4 2.9414498523980465E-5 0.0 4.044493547047314E-5 2.206087389298535E-5 18-19 2.1693192661435592E-4 2.9414498523980465E-5 0.0 5.1475372416965814E-5 2.9414498523980465E-5 20-21 2.647304867158242E-4 2.9414498523980465E-5 0.0 5.882899704796093E-5 3.676812315497558E-5 22-23 2.720841113468193E-4 2.9414498523980465E-5 0.0 5.882899704796093E-5 3.676812315497558E-5 24-25 3.051754221862973E-4 2.9414498523980465E-5 0.0 5.882899704796093E-5 3.676812315497558E-5 26-27 3.603276069187607E-4 2.9414498523980465E-5 0.0 5.882899704796093E-5 4.41217477859707E-5 28-29 3.934189177582387E-4 2.9414498523980465E-5 0.0 5.882899704796093E-5 4.41217477859707E-5 30-31 4.2283341628221915E-4 2.9414498523980465E-5 0.0 5.882899704796093E-5 4.41217477859707E-5 32-33 4.669551640681899E-4 2.9414498523980465E-5 0.0 5.882899704796093E-5 4.41217477859707E-5 34-35 5.147537241696581E-4 3.676812315497558E-5 0.0 5.882899704796093E-5 5.515218473246337E-5 36-37 5.772595335331167E-4 3.676812315497558E-5 0.0 6.25058093634585E-5 6.618262167895605E-5 38-39 6.65503029105058E-4 3.676812315497558E-5 0.0 6.618262167895605E-5 7.721305862544873E-5 40-41 7.206552138375214E-4 3.676812315497558E-5 0.0 6.618262167895605E-5 8.088987094094628E-5 42-43 7.831610232009799E-4 3.676812315497558E-5 0.0 7.353624630995116E-5 9.559712020293651E-5 44-45 8.677277064574237E-4 3.676812315497558E-5 0.0 8.82434955719414E-5 9.559712020293651E-5 46-47 9.486175773983699E-4 3.676812315497558E-5 0.0 9.559712020293651E-5 9.559712020293651E-5 48-49 0.0010331842606548138 4.044493547047314E-5 0.0 9.559712020293651E-5 9.559712020293651E-5 50-51 0.0011361350054887454 4.41217477859707E-5 0.0 1.0295074483393163E-4 1.0295074483393163E-4 52-53 0.0012427625626381747 4.41217477859707E-5 0.0 1.1765799409592186E-4 1.0295074483393163E-4 54-55 0.0013236524335791208 4.41217477859707E-5 0.0 1.323652433579121E-4 1.0295074483393163E-4 56-57 0.0014118959291510623 4.41217477859707E-5 0.0 1.3604205567340963E-4 1.0295074483393163E-4 58-59 0.001489108987776511 5.1475372416965814E-5 0.0 1.397188679889072E-4 1.1030436946492674E-4 60-61 0.0015957365449259403 5.1475372416965814E-5 0.0 1.397188679889072E-4 1.139811817804243E-4 62-63 0.0017097177267063645 5.1475372416965814E-5 0.0 1.397188679889072E-4 1.1765799409592186E-4 64-65 0.001805314846909301 5.1475372416965814E-5 0.0 1.397188679889072E-4 1.1765799409592186E-4 66-67 0.0019450337148982084 5.1475372416965814E-5 0.0 1.397188679889072E-4 1.1765799409592186E-4 68-69 0.002084752582887116 5.1475372416965814E-5 0.0 1.397188679889072E-4 1.3604205567340963E-4 70-71 0.0022502091370845056 5.1475372416965814E-5 0.0 1.397188679889072E-4 1.507493049353999E-4 72-73 0.0024597874390678664 5.1475372416965814E-5 0.0 1.397188679889072E-4 1.58102929566395E-4 74-75 0.0026767193656822225 5.1475372416965814E-5 0.0 1.4707249261990233E-4 1.6177974188189256E-4 76-77 0.0029304194154515534 5.1475372416965814E-5 0.0 1.4707249261990233E-4 1.6177974188189256E-4 78-79 0.0031988267144828757 5.1475372416965814E-5 0.0 1.4707249261990233E-4 1.6177974188189256E-4 80-81 0.0036069528815031045 5.515218473246337E-5 0.0 1.4707249261990233E-4 1.6177974188189256E-4 82-83 0.004224657350506695 5.882899704796093E-5 0.0 1.507493049353999E-4 1.6177974188189256E-4 84-85 0.004989434312130186 5.882899704796093E-5 0.0 1.5442611725089743E-4 1.654565541973901E-4 86-87 0.006213812813190873 5.882899704796093E-5 0.0 1.654565541973901E-4 1.6913336651288766E-4 88-89 0.007842640668956291 6.618262167895605E-5 0.0 1.764869911438828E-4 1.7281017882838523E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AAGCAGT 470070 0.0 92.3337 1 AGTGGTA 468925 0.0 91.77531 5 AGCAGTG 473495 0.0 91.4396 2 GGTATCA 472420 0.0 91.37933 8 TGGTATC 471555 0.0 91.339035 7 GTGGTAT 472820 0.0 91.28027 6 GTATCAA 473680 0.0 91.24894 9 GCAGTGG 474385 0.0 91.076355 3 CAGTGGT 475235 0.0 90.663086 4 GTATCGA 975 0.0 47.76268 9 ATCAACG 473715 0.0 46.06334 10-11 TCAACGC 474465 0.0 46.038803 12-13 CAACGCA 474630 0.0 46.026806 12-13 AACGCAG 476820 0.0 45.88905 14-15 ACGCAGA 476935 0.0 45.882465 14-15 CGCAGAG 477340 0.0 45.806934 16-17 TATCAAC 475580 0.0 45.78529 10-11 AGAGTAC 464835 0.0 45.698425 18-19 CAGAGTA 473135 0.0 45.646755 18-19 GCAGAGT 477635 0.0 45.49618 16-17 >>END_MODULE