FastQCFastQC Report
Sat 18 Jun 2016
SRR578606_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR578606_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13598736
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4337403.189561147447822No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT312840.23005079295605121No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT297700.21891740526472458No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT158850.028.5239031
CGTCACG9700.026.429523
AGTGGTA171950.025.37375
GGTATCA185800.023.2777778
CGCGTAG5350.023.07344254-55
GTATCAA189850.023.0562789
TGGTATC201200.021.732047
AGCAGTG210800.021.4629552
GTGGTAT210700.020.8648536
ACTCGCG6300.019.9738650-51
GCAGTGG221350.019.968133
CTCGCGT6450.019.50802452-53
ATCAACG170150.019.35010710-11
ACGTACG7000.019.32949320-21
CAACGCA172500.019.1277112-13
CGCTAGT7150.018.26038490-91
CAGTGGT245850.018.0362854
ACGCAGA195850.016.76238614-15
GTGTACG15100.015.7203399
AGAGTAC203200.015.56046518-19
Produced by FastQC (version 0.11.5)