FastQCFastQC Report
Sat 18 Jun 2016
SRR578605_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR578605_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12193169
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT190690415.639117279519377No Hit
AAGCAGTGGTATCAACGCAGAGTATTTTTTTTTTTTTTTTTTTTTTTTTT445370.36526189377019214No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA311260.25527408010173563No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAG258300.21183992446918437No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGCCAGCAGCTGACCCAGC141960.11642584466761677No Hit
AAGCAGTGGTATCAACGCAGAGTACCTTTTTTTTTTTTTTTTTTTTTTTT127620.10466516128825902No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT4150850.092.236291
AGTGGTA4149850.091.707875
AGCAGTG4185600.091.3690952
GGTATCA4178000.091.251918
TGGTATC4181200.091.177537
GTATCAA4180950.091.1701059
GTGGTAT4187250.091.105516
GCAGTGG4190800.091.071353
CAGTGGT4206900.090.561354
ATCAACG4178250.046.0000310-11
CAACGCA4186750.045.98128512-13
TCAACGC4185000.045.96988712-13
AACGCAG4200500.045.8316214-15
ACGCAGA4204400.045.83144414-15
TATCAAC4199000.045.70940810-11
CGCAGAG4214000.045.69494216-17
AGAGTAC4115850.045.6096218-19
CAGAGTA4192850.045.50606518-19
GAGTACT2309000.045.38592520-21
GCAGAGT4232500.045.35336316-17
Produced by FastQC (version 0.11.5)