##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR578605_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	12193169
Sequences flagged as poor quality	0
Sequence length	101
%GC	42
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.991963204971572	31.0	31.0	34.0	27.0	34.0
2	31.340959843991335	33.0	31.0	34.0	28.0	34.0
3	31.48027407805141	34.0	31.0	34.0	28.0	34.0
4	34.85389245404538	37.0	35.0	37.0	32.0	37.0
5	34.87998780300675	37.0	35.0	37.0	32.0	37.0
6	34.93412582077719	37.0	35.0	37.0	32.0	37.0
7	34.843511477615046	37.0	35.0	37.0	32.0	37.0
8	34.89681460168394	37.0	35.0	37.0	32.0	37.0
9	36.38238434979454	39.0	37.0	39.0	32.0	39.0
10-11	36.24941809631278	39.0	37.0	39.0	32.0	39.0
12-13	36.34512656225793	39.0	37.0	39.0	32.0	39.0
14-15	37.61361738691558	40.0	38.0	41.0	32.0	41.0
16-17	37.573025806498705	40.0	38.0	41.0	32.0	41.0
18-19	37.465630345974866	40.0	38.0	41.0	31.0	41.0
20-21	37.35648337196015	40.0	37.5	41.0	31.0	41.0
22-23	37.07537175118298	39.5	37.0	41.0	30.5	41.0
24-25	37.04773238195911	40.0	37.0	41.0	31.0	41.0
26-27	36.94821042831441	40.0	37.0	41.0	30.0	41.0
28-29	36.79569585232518	40.0	36.5	41.0	30.0	41.0
30-31	36.53092698870982	40.0	36.0	41.0	29.5	41.0
32-33	36.22781542681808	39.0	35.5	41.0	28.5	41.0
34-35	36.06233088379239	39.0	35.0	41.0	27.0	41.0
36-37	35.73803709273611	39.0	35.0	40.5	27.0	41.0
38-39	34.68337328876521	37.0	34.0	40.0	25.5	41.0
40-41	34.4428985606613	37.0	34.0	40.0	25.0	41.0
42-43	34.12896372550893	36.5	33.5	40.0	24.0	41.0
44-45	33.84909161022864	36.0	33.0	40.0	23.0	41.0
46-47	33.69791229006995	36.0	33.0	40.0	23.0	41.0
48-49	33.70581273826353	36.0	33.0	40.0	22.5	41.0
50-51	32.81538080871347	36.0	32.0	39.5	19.0	40.5
52-53	32.33855427575883	36.0	32.0	39.5	11.0	40.5
54-55	31.714046405819523	36.5	31.0	40.0	2.0	41.0
56-57	31.083006435816642	36.0	30.0	40.0	2.0	41.0
58-59	30.83722541695272	36.0	30.0	40.0	2.0	41.0
60-61	30.61504671181052	35.0	30.0	40.0	2.0	41.0
62-63	30.29502063819504	35.0	29.0	39.0	2.0	41.0
64-65	30.00432775105471	35.0	29.0	39.0	2.0	41.0
66-67	29.674103016205223	35.0	29.0	38.5	2.0	41.0
68-69	29.338051330216125	35.0	28.5	37.5	2.0	40.0
70-71	29.045248901249543	35.0	28.0	37.0	2.0	40.0
72-73	28.730984086253542	35.0	28.0	36.5	2.0	39.0
74-75	28.373144545113743	35.0	27.0	36.0	2.0	39.0
76-77	28.010241103030722	35.0	26.0	36.0	2.0	38.5
78-79	27.684341207769695	34.5	26.0	35.0	2.0	37.0
80-81	27.38294540164251	34.0	26.0	35.0	2.0	37.0
82-83	27.133924863995567	34.0	25.0	35.0	2.0	36.5
84-85	26.87309513220066	34.0	25.0	35.0	2.0	36.0
86-87	26.64323130434754	34.0	24.5	35.0	2.0	36.0
88-89	26.392237120636974	34.0	24.0	35.0	2.0	35.5
90-91	26.155001214204447	34.0	23.0	35.0	2.0	35.0
92-93	25.848513827701396	34.0	20.0	35.0	2.0	35.0
94-95	25.565864788718994	34.0	18.5	35.0	2.0	35.0
96-97	25.281828784625226	34.0	11.5	35.0	2.0	35.0
98-99	24.960145840675217	34.0	2.0	35.0	2.0	35.0
100-101	24.115874183323466	32.5	2.0	34.5	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.6032632955746067
1101	2	0.6328706313332617
1101	3	0.5260360286938948
1101	4	0.6241418550773759
1101	5	0.6125176443013629
1101	6	0.5654098681885529
1101	7	0.5557827991910642
1101	8	0.5199644375490706
1101	9	0.6589307744450679
1101	10-11	0.7555826659386611
1101	12-13	0.7327699831408054
1101	14-15	0.8910792027081342
1101	16-17	0.8065511736977058
1101	18-19	0.85255604292405
1101	20-21	0.8994389476055673
1101	22-23	0.9420362573769481
1101	24-25	0.8820143848671265
1101	26-27	0.9492564037712938
1101	28-29	1.0655486233547578
1101	30-31	1.1373019489060994
1101	32-33	1.1518611308740176
1101	34-35	1.2102181523966777
1101	36-37	1.2049402895529866
1101	38-39	1.2345751252727553
1101	40-41	1.1730365615659224
1101	42-43	1.2056178626594374
1101	44-45	1.3539374443842291
1101	46-47	1.2431492290162822
1101	48-49	1.192675360747927
1101	50-51	1.28822932534122
1101	52-53	1.0866442629850965
1101	54-55	0.9170576007379552
1101	56-57	0.8941306950730663
1101	58-59	0.9044125148116464
1101	60-61	0.8112990905399826
1101	62-63	0.8268447867917921
1101	64-65	0.9062206341257699
1101	66-67	0.8627362359604653
1101	68-69	0.7472595470993078
1101	70-71	0.8371245853827425
1101	72-73	0.8018424237385666
1101	74-75	0.7577302095300276
1101	76-77	0.7611185080487424
1101	78-79	0.7727858503227196
1101	80-81	0.7702100006087953
1101	82-83	0.8260989825263216
1101	84-85	0.8197807474248187
1101	86-87	0.840088364176399
1101	88-89	0.8058761770070504
1101	90-91	0.8200741773640381
1101	92-93	0.9109381945032666
1101	94-95	0.9957280261227837
1101	96-97	1.0325393285046154
1101	98-99	1.0552498069601874
1101	100-101	1.1833904071482522
1102	1	0.5552231451944394
1102	2	0.5265546715643481
1102	3	0.5241074542284068
1102	4	0.5391082414927197
1102	5	0.5895757692477659
1102	6	0.6048439104013639
1102	7	0.6315306465020143
1102	8	0.5132849941891706
1102	9	0.6559636342552722
1102	10-11	0.6785377464577707
1102	12-13	0.658502841437695
1102	14-15	0.8293155650473665
1102	16-17	0.8396065617077895
1102	18-19	0.9279503873096218
1102	20-21	0.9186514406605468
1102	22-23	0.9492673937387863
1102	24-25	0.9449002528931842
1102	26-27	1.0492186429858705
1102	28-29	1.105487049493874
1102	30-31	1.0562760049405142
1102	32-33	1.1610863105440217
1102	34-35	1.1116030868521705
1102	36-37	1.1605081137606774
1102	38-39	1.1499390448422773
1102	40-41	1.1830159840997396
1102	42-43	1.1799382586000178
1102	44-45	1.2363167413850533
1102	46-47	1.3222552099932017
1102	48-49	1.244370884869788
1102	50-51	1.2310848442169302
1102	52-53	1.1429253264556465
1102	54-55	1.08680441971606
1102	56-57	1.024469126112436
1102	58-59	1.054553325068742
1102	60-61	1.1148494330893968
1102	62-63	1.1400484687566212
1102	64-65	1.1014433002534112
1102	66-67	1.0732531731860426
1102	68-69	1.019282101962343
1102	70-71	1.0106081907883002
1102	72-73	1.0071451477756845
1102	74-75	0.9574844221275711
1102	76-77	1.0529838331986525
1102	78-79	1.072973375139263
1102	80-81	1.1206771642189288
1102	82-83	1.1318375950192348
1102	84-85	1.104533533183524
1102	86-87	1.1023610157834263
1102	88-89	1.1412595642792134
1102	90-91	1.1307855754282947
1102	92-93	1.0918233101961192
1102	94-95	1.2192976201871382
1102	96-97	1.2812903400829079
1102	98-99	1.3345010480577564
1102	100-101	1.3928057599341557
1103	1	0.4649387543655088
1103	2	0.4916624753466259
1103	3	0.4064608972984587
1103	4	0.5048621314426711
1103	5	0.520885252062115
1103	6	0.5762595525254426
1103	7	0.4640845598682688
1103	8	0.4829148891784314
1103	9	0.590606843381579
1103	10-11	0.5822879462572104
1103	12-13	0.6263046973066224
1103	14-15	0.6827650302733659
1103	16-17	0.7897714582582012
1103	18-19	0.7852949733546595
1103	20-21	0.801526605226961
1103	22-23	0.8892018032050686
1103	24-25	0.7924806608925579
1103	26-27	0.819422564820762
1103	28-29	0.8753165073623776
1103	30-31	0.9397589349623203
1103	32-33	1.0162274481292215
1103	34-35	1.0566738567241387
1103	36-37	1.0688458094039177
1103	38-39	0.9096997980068267
1103	40-41	1.0496503068247378
1103	42-43	0.9346722987511242
1103	44-45	0.9036467713322907
1103	46-47	1.1933255737872628
1103	48-49	1.244669289111755
1103	50-51	1.2136801880522157
1103	52-53	1.1606665575153912
1103	54-55	1.0452541292737862
1103	56-57	1.0317849775814807
1103	58-59	1.0085267282541146
1103	60-61	1.0642447376691955
1103	62-63	0.9171982472109477
1103	64-65	0.8519494773795735
1103	66-67	0.7768105583578091
1103	68-69	0.9806936734210225
1103	70-71	0.9009161259094682
1103	72-73	0.9501738684756056
1103	74-75	0.9178038272461997
1103	76-77	0.9587609350155297
1103	78-79	0.9764370748067108
1103	80-81	1.0444234547107776
1103	82-83	1.0346105032280768
1103	84-85	1.0608469916846097
1103	86-87	1.0806892455142822
1103	88-89	1.0852519361862782
1103	90-91	1.0245424814627242
1103	92-93	1.0587001047940987
1103	94-95	1.0034644451221482
1103	96-97	0.9394563520853154
1103	98-99	1.0209789907111961
1103	100-101	1.0816456471305784
1104	1	0.33361857607142653
1104	2	0.35253572761387986
1104	3	0.3581484071767136
1104	4	0.33305874954267267
1104	5	0.43653224909285626
1104	6	0.40189923190632726
1104	7	0.40847086813675304
1104	8	0.32882397374557826
1104	9	0.49933015442470463
1104	10-11	0.5197329428678117
1104	12-13	0.5349795205414623
1104	14-15	0.6356619069882683
1104	16-17	0.6326693875315783
1104	18-19	0.6981058841677168
1104	20-21	0.7147466980197521
1104	22-23	0.6992927637354214
1104	24-25	0.6866280280496255
1104	26-27	0.709922697673413
1104	28-29	0.7343638029296216
1104	30-31	0.8576739739642676
1104	32-33	0.8803985370088085
1104	34-35	0.872168514677881
1104	36-37	0.8814107159155427
1104	38-39	0.9314568589006669
1104	40-41	0.8594449439031138
1104	42-43	0.9735533550289404
1104	44-45	0.9576504614180692
1104	46-47	1.1213052403148467
1104	48-49	1.0272196965139102
1104	50-51	1.0001095886624825
1104	52-53	0.86126676340308
1104	54-55	0.8249730921483049
1104	56-57	0.8258090901853095
1104	58-59	0.7885921558847038
1104	60-61	0.7878009062316131
1104	62-63	0.6767494094539188
1104	64-65	0.5915636653060581
1104	66-67	0.7353865700431435
1104	68-69	0.7034464062432377
1104	70-71	0.6126471393730455
1104	72-73	0.6629850074625843
1104	74-75	0.7472261490615217
1104	76-77	0.7613584557076329
1104	78-79	0.6861847578748197
1104	80-81	0.8055544476142167
1104	82-83	0.7906416338768132
1104	84-85	0.7703274936330935
1104	86-87	0.7839797553974073
1104	88-89	0.7706844014103389
1104	90-91	0.7960999530852568
1104	92-93	0.6721108610412614
1104	94-95	0.5167850728406407
1104	96-97	0.6533905513784646
1104	98-99	0.9250426085859438
1104	100-101	0.8684116382789995
1105	1	0.23337506450436152
1105	2	0.1548397861447306
1105	3	0.17429124305121135
1105	4	0.1872488915682382
1105	5	0.1296491815247407
1105	6	0.13977337443422044
1105	7	0.15259858346509958
1105	8	0.1602944432357134
1105	9	0.1298875993737454
1105	10-11	0.17271727539197457
1105	12-13	0.1312058867860415
1105	14-15	0.014958501420444748
1105	16-17	0.06250283607530349
1105	18-19	-0.054440659549158
1105	20-21	0.0500561345031727
1105	22-23	-0.00558424030020177
1105	24-25	0.006359397493390873
1105	26-27	-0.2853435015317345
1105	28-29	-0.1319059739060151
1105	30-31	-0.32929697810253344
1105	32-33	-0.28389637259579104
1105	34-35	-0.14119913955913432
1105	36-37	-0.08530538040999858
1105	38-39	-0.06712547447472161
1105	40-41	-0.02857045564820737
1105	42-43	0.055336000623043446
1105	44-45	0.029904082376752683
1105	46-47	0.03714874227868137
1105	48-49	0.07846500215242713
1105	50-51	0.00931659956243891
1105	52-53	-0.10342872958371885
1105	54-55	-0.20663239439467063
1105	56-57	-0.02231895770470871
1105	58-59	-0.050510824015557176
1105	60-61	-0.07746196948777495
1105	62-63	-0.07171412653035958
1105	64-65	-0.17974578097948068
1105	66-67	-0.15414861927124335
1105	68-69	-0.11415429913979835
1105	70-71	-0.11535566800996477
1105	72-73	-0.133141480186179
1105	74-75	-0.10948469958016105
1105	76-77	-0.09688654848028477
1105	78-79	-0.051899366588763485
1105	80-81	-0.006451895065502811
1105	82-83	-0.030134602127468213
1105	84-85	-0.10264875995817135
1105	86-87	-0.04371890580393867
1105	88-89	0.042845886024707625
1105	90-91	0.03943172589321975
1105	92-93	0.09652961683729444
1105	94-95	0.1346770644545785
1105	96-97	0.14026077604297527
1105	98-99	0.19277257760557376
1105	100-101	0.1724979062808032
1106	1	-0.10468666700696261
1106	2	-0.05113848578943703
1106	3	-0.0760743115602196
1106	4	-0.10224043655040305
1106	5	-0.14281173613946407
1106	6	-0.1105621061724733
1106	7	-0.23810224804675073
1106	8	-0.17230260593608904
1106	9	-0.12144144594839901
1106	10-11	-0.29694036359565246
1106	12-13	-0.324071045781146
1106	14-15	-0.30416444444922064
1106	16-17	-0.30146200098930365
1106	18-19	-0.319689345120608
1106	20-21	-0.10548217331386667
1106	22-23	-0.12034587392672336
1106	24-25	-0.26570786631830146
1106	26-27	-0.24229399633462378
1106	28-29	-0.2730544758150444
1106	30-31	-0.2901155118165377
1106	32-33	-0.225011242105289
1106	34-35	-0.2168232968169974
1106	36-37	-0.28987890774882175
1106	38-39	-0.3467988475680386
1106	40-41	-0.3623014658272652
1106	42-43	-0.44842617391239514
1106	44-45	-0.3588587169715538
1106	46-47	-0.41774280722808044
1106	48-49	-0.5964435565931225
1106	50-51	-0.6603836611134142
1106	52-53	-0.7405754346694096
1106	54-55	-0.7525126204303483
1106	56-57	-0.8117759358447358
1106	58-59	-0.7605519605757287
1106	60-61	-0.8921238243607021
1106	62-63	-0.7682883785486645
1106	64-65	-0.7369629414593071
1106	66-67	-0.8749945781619282
1106	68-69	-0.9913570671777627
1106	70-71	-0.8754047670992797
1106	72-73	-0.9321699481908681
1106	74-75	-1.0252966903027207
1106	76-77	-1.0357375792105223
1106	78-79	-0.935203210999866
1106	80-81	-0.9779274097829465
1106	82-83	-1.040533209404245
1106	84-85	-1.079027445313816
1106	86-87	-1.0471116511358822
1106	88-89	-1.115430929690536
1106	90-91	-1.0594316880156391
1106	92-93	-1.0122935689614465
1106	94-95	-1.02587459523318
1106	96-97	-0.9724417844567306
1106	98-99	-1.0572678639083222
1106	100-101	-1.169294353590761
1107	1	-0.39278334154726835
1107	2	-0.4805989994291018
1107	3	-0.4435763974083109
1107	4	-0.30305811886321266
1107	5	-0.38504701560405863
1107	6	-0.3889428314452559
1107	7	-0.3411306744818958
1107	8	-0.3168986687198725
1107	9	-0.3685781006393114
1107	10-11	-0.4153764037255314
1107	12-13	-0.4771832282638968
1107	14-15	-0.6480592467396704
1107	16-17	-0.5204652165735624
1107	18-19	-0.5073409327272174
1107	20-21	-0.42912452380650024
1107	22-23	-0.5098727904319773
1107	24-25	-0.4951910295353841
1107	26-27	-0.4180095514187343
1107	28-29	-0.47615575284443423
1107	30-31	-0.47570084537990454
1107	32-33	-0.3735350605659633
1107	34-35	-0.5001161124483673
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2308	56-57	-2.0942125499016093
2308	58-59	-2.0080160939818725
2308	60-61	-1.980775400215073
2308	62-63	-2.051112160834812
2308	64-65	-1.9900236254896022
2308	66-67	-2.1976606555192397
2308	68-69	-2.031075689763732
2308	70-71	-1.9990639709989715
2308	72-73	-1.9217615006944122
2308	74-75	-1.7564605252229022
2308	76-77	-1.7049206612347767
2308	78-79	-1.7724643524943886
2308	80-81	-2.365267602498829
2308	82-83	-2.2259425661539503
2308	84-85	-1.933357796751114
2308	86-87	-1.823877241819158
2308	88-89	-1.7121537670930245
2308	90-91	-1.9637495732242982
2308	92-93	-1.9733887688796656
2308	94-95	-2.0691965417838105
2308	96-97	-2.013829433302675
2308	98-99	-2.0220484298865777
2308	100-101	-2.162191242511671
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	111002.0
3	20531.0
4	18039.0
5	17876.0
6	20949.0
7	25835.0
8	31806.0
9	36901.0
10	42468.0
11	51622.0
12	49700.0
13	54062.0
14	59451.0
15	68887.0
16	85253.0
17	112012.0
18	164379.0
19	267838.0
20	430186.0
21	314851.0
22	162417.0
23	145952.0
24	154386.0
25	158446.0
26	163229.0
27	179984.0
28	204497.0
29	234652.0
30	276596.0
31	329106.0
32	403097.0
33	507843.0
34	674995.0
35	1035958.0
36	1752971.0
37	1945836.0
38	1607392.0
39	271942.0
40	222.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	10.506362974699904	52.284885996532346	20.475305060921922	16.733445967845828
2	15.841556930909467	53.25930282718275	16.361130413403803	14.538009828503975
3	49.63990683157164	18.179351276608894	16.737238487061212	15.44350340475825
4	15.47005673574613	17.09948314105358	17.737115103708636	49.69334501949166
5	15.668837260002672	51.03928934820146	18.555429257733252	14.736444134062621
6	49.48112184692967	17.859719269261568	16.97048216703371	15.688676716775053
7	16.24572461291057	17.44269357424001	51.66879520198723	14.64278661086219
8	49.09580789064052	18.106224621382957	17.656205646754337	15.141761841222179
9	48.65983849459787	17.859147092553354	18.265404028432325	15.215610384416452
10-11	15.15569908286378	34.71436149565771	34.59682567153003	15.53311374994848
12-13	15.001468893478831	18.288812716142967	34.414375773178726	32.295342617199466
14-15	15.258681159900537	51.65094517595591	17.482777774314613	15.607595889828938
16-17	32.11207471888501	18.02753602607593	17.584425533698266	32.2759637213408
18-19	15.483698527552589	34.75394815478031	17.58657211155718	32.17578120610992
20-21	32.211358939431875	34.78057334261832	17.72568364746703	15.282384070482777
22-23	32.07066308169651	17.994398156076112	34.551888571989075	15.383050190238304
24-25	15.281911211071225	34.479197246669024	18.293478709716258	31.945412832543497
26-27	15.528527236989968	24.8898918675891	44.01349412048571	15.568086774935223
28-29	29.795641728145938	17.777986185185956	37.02980185841086	15.396570228257234
30-31	26.56419364913561	19.62343495337503	37.57401205714151	16.23835934034786
32-33	19.46608067658956	22.355181633495455	39.530378237687984	18.648359452227
34-35	18.808137571531894	21.29211174978423	40.38766657695599	19.512084101727886
36-37	19.029790904053424	21.096224272765955	40.46867932113764	19.405305502042975
38-39	19.23032603379826	21.05357086764674	40.32795541199997	19.388147686555026
40-41	19.22563614991082	21.056239623728217	39.90759697386548	19.810527252495483
42-43	19.688607496935138	21.198588276127456	39.918482976676195	19.194321250261215
44-45	19.059812131374358	21.343678532808937	39.78655542337692	19.809953912439788
46-47	19.608160418585964	21.17865331654891	39.62260963232254	19.590576632542586
48-49	19.367758846442946	21.40739750485836	39.757268474796184	19.467575173902507
50-51	19.53178259554446	21.092981606620643	39.463962217231234	19.911273580603655
52-53	20.038169811687194	22.16784838206141	37.93512741548921	19.858854390762186
54-55	20.67782530210525	23.07859089274011	36.10643533050487	20.13714847464977
56-57	21.0518787055837	23.962559737281598	34.785799791852554	20.199761765282144
58-59	21.092542973430305	24.837893047264203	33.865503410548	20.20406056875749
60-61	20.93918697718524	25.07813422029468	33.538793099024744	20.443885703495333
62-63	20.79072021498256	25.12406716398763	33.43165216734468	20.65356045368513
64-65	20.57421231665217	24.688545718503534	33.774061519505	20.963180445339297
66-67	20.59481450752832	24.09883034424226	34.704056006576955	20.60229914165247
68-69	20.46802045931124	24.036754787031093	34.94269490867599	20.552529844981677
70-71	20.44253636557424	24.00669027373004	35.295985244545285	20.25478811615043
72-73	20.061554539284977	23.793593554233354	35.34480136162395	20.800050544857722
74-75	20.665994945587638	23.93219011014835	34.742840472018855	20.658974472245152
76-77	21.05434940243644	24.50522620729322	33.85250501712944	20.587919373140902
78-79	20.773308694966374	24.574713645305334	33.7072111615449	20.944766498183395
80-81	20.692863091247524	25.284731858346433	33.191065097059656	20.831339953346387
82-83	21.676378104750192	25.29555316114309	32.3969575872336	20.63111114687312
84-85	21.410575394608045	25.860713125294733	31.988219873470218	20.740491606627003
86-87	21.13499712768924	26.116875622210827	31.907418805900438	20.840708444199493
88-89	20.942298109151945	25.65576749999035	31.889839747213628	21.51209464364408
90-91	20.966596915407834	25.784968546238908	31.894461106241494	21.353973432111758
92-93	21.251709893626238	25.96454426997244	31.941687648069294	20.84205818833203
94-95	21.08098015945003	25.82002663072314	31.29466869285008	21.80432451697675
96-97	21.044951225790935	25.52248752326835	31.753349812908578	21.679211438032137
98-99	21.42157749829393	25.98903721300622	31.134620571993743	21.45476471670611
100-101	21.812446277126778	25.579205196073218	30.979587001851545	21.628761524948455
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	35755.0
1	20718.0
2	3917.5
3	1755.0
4	1298.5
5	1186.5
6	1496.0
7	1919.0
8	1664.5
9	1837.0
10	3477.0
11	21759.5
12	378931.5
13	408567.5
14	84872.0
15	72738.5
16	76898.0
17	82806.5
18	87765.5
19	90424.0
20	91594.0
21	91985.5
22	92085.5
23	92503.5
24	94585.0
25	98780.5
26	104659.5
27	111886.0
28	117517.5
29	123274.0
30	131597.5
31	141453.5
32	150866.0
33	161532.5
34	181759.5
35	195065.0
36	203257.5
37	217042.0
38	228138.0
39	240450.0
40	256313.5
41	274572.0
42	289592.0
43	303174.0
44	319097.5
45	332470.5
46	340408.5
47	360375.5
48	375757.0
49	379862.5
50	386602.5
51	386511.5
52	390392.0
53	398441.0
54	385149.5
55	361190.0
56	346801.5
57	330677.0
58	339374.5
59	301161.0
60	223431.5
61	182641.0
62	145845.5
63	113558.5
64	89493.0
65	72571.5
66	57716.5
67	45832.0
68	35655.0
69	27301.5
70	21801.5
71	18200.5
72	15414.5
73	12176.5
74	9401.5
75	7109.5
76	5316.5
77	3995.5
78	3022.5
79	2337.0
80	1890.0
81	1675.0
82	1452.5
83	1183.5
84	976.0
85	834.5
86	649.0
87	491.5
88	371.0
89	246.5
90	168.0
91	134.5
92	106.5
93	87.5
94	64.0
95	46.0
96	39.0
97	29.5
98	18.5
99	13.0
100	5.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05133202041241289
2	0.09229758071917153
3	0.08260362831024487
4	0.05471916283617491
5	0.08467856059405066
6	0.0010907746788386186
7	9.92358918341901E-4
8	0.0046911512503435325
9	0.00927568542681562
10-11	0.00936179921725025
12-13	0.016562552360260076
14-15	0.011194792756501612
16-17	0.0013163107966435961
18-19	5.576893094813989E-4
20-21	4.920788024835873E-5
22-23	3.3625384836378467E-4
24-25	6.84809666789659E-4
26-27	0.0012712035730826005
28-29	0.001402424587078224
30-31	0.004600936803221541
32-33	1.312210139956233E-4
34-35	0.0022020526411140533
36-37	2.8294531142806275E-4
38-39	0.0023947835054201247
40-41	0.003169807619331775
42-43	0.0010907746788386186
44-45	6.068971897297577E-4
46-47	9.677549782177218E-4
48-49	0.002247159864675049
50-51	0.004272884268232483
52-53	0.12912557842838068
54-55	0.1390040603882387
56-57	0.12517664603844988
58-59	0.1187632189794138
60-61	0.10667038240837964
62-63	0.11134513103197373
64-65	0.11394494737176201
66-67	0.12207654958280328
68-69	0.13462455904613477
70-71	0.1221421600898011
72-73	0.11390394080488839
74-75	0.11894364787365778
76-77	0.1302942655842792
78-79	0.14416678715762898
80-81	0.13454664656907486
82-83	0.11389984014820102
84-85	0.12049369610148108
86-87	0.1215516655268208
88-89	0.11767244430057518
90-91	0.1280307030928547
92-93	0.14519605198615718
94-95	0.15487770242502175
96-97	0.1550622319759531
98-99	0.13773695747184345
100-101	0.12721467241206943
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	1.2193169E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.030946520928914
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.62043029115864	35.4755970564708
2	6.017911684346684	4.8180540160751075
3	1.9163214164828895	2.301364804204119
4	0.9694676696283097	1.5523483374664175
5	0.5832593936226015	1.1674212796967893
6	0.381516769071244	0.9163486427717132
7	0.24944666497734774	0.6989910273872594
8	0.19286126388614716	0.6176335152468093
9	0.14764475235962352	0.5319323267223495
>10	0.8882216895981361	6.319149961752851
>50	0.01405962433164782	0.3676285546505244
>100	0.01032019568650302	0.9329699552946679
>500	0.0041285465229723844	1.1704094354612802
>1k	0.0035655629029924226	3.0797055572150467
>5k	4.222377121964711E-4	1.2281974614758382
>10k+	4.222377121964711E-4	38.822248068108436
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1906904	15.639117279519377	No Hit
AAGCAGTGGTATCAACGCAGAGTATTTTTTTTTTTTTTTTTTTTTTTTTT	44537	0.36526189377019214	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	31126	0.25527408010173563	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAG	25830	0.21183992446918437	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGCCAGCAGCTGACCCAGC	14196	0.11642584466761677	No Hit
AAGCAGTGGTATCAACGCAGAGTACCTTTTTTTTTTTTTTTTTTTTTTTT	12762	0.10466516128825902	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	8.201313374726455E-6	0.0	0.0	0.0	0.0
2	3.280525349890582E-5	0.0	0.0	0.0	0.0
3	4.920788024835873E-5	0.0	0.0	0.0	8.201313374726455E-6
4	4.920788024835873E-5	1.640262674945291E-5	0.0	0.0	8.201313374726455E-6
5	5.740919362308519E-5	2.4603940124179366E-5	0.0	0.0	8.201313374726455E-6
6	5.740919362308519E-5	2.4603940124179366E-5	0.0	0.0	8.201313374726455E-6
7	5.740919362308519E-5	2.4603940124179366E-5	0.0	8.201313374726455E-6	1.640262674945291E-5
8	5.740919362308519E-5	2.4603940124179366E-5	0.0	8.201313374726455E-6	1.640262674945291E-5
9	9.021444712199102E-5	2.4603940124179366E-5	0.0	8.201313374726455E-6	1.640262674945291E-5
10-11	1.0661707387144393E-4	2.4603940124179366E-5	0.0	8.201313374726455E-6	1.640262674945291E-5
12-13	1.1891904393353361E-4	2.4603940124179366E-5	0.0	8.201313374726455E-6	2.050328343681614E-5
14-15	1.312210139956233E-4	2.8704596811542594E-5	0.0	1.2301970062089683E-5	2.8704596811542594E-5
16-17	1.3942232737034974E-4	3.280525349890582E-5	0.0	1.640262674945291E-5	4.920788024835873E-5
18-19	1.5992561080716588E-4	3.280525349890582E-5	0.0	1.640262674945291E-5	4.920788024835873E-5
20-21	1.8042889424398203E-4	3.280525349890582E-5	0.0	2.050328343681614E-5	4.920788024835873E-5
22-23	1.8863020761870848E-4	3.280525349890582E-5	0.0	3.280525349890582E-5	5.330853693572196E-5
24-25	2.214354611176143E-4	3.280525349890582E-5	0.0	3.280525349890582E-5	5.740919362308519E-5
26-27	2.542407146165201E-4	3.280525349890582E-5	0.0	3.280525349890582E-5	5.740919362308519E-5
28-29	3.0344859486487887E-4	3.280525349890582E-5	0.0	3.6905910186269046E-5	5.740919362308519E-5
30-31	3.4855581842587434E-4	3.280525349890582E-5	0.0	4.100656687363228E-5	5.740919362308519E-5
32-33	4.018643553615963E-4	3.280525349890582E-5	0.0	5.330853693572196E-5	6.561050699781164E-5
34-35	4.3466960886050216E-4	4.100656687363228E-5	0.0	6.561050699781164E-5	7.381182037253809E-5
36-37	4.756761757341344E-4	4.100656687363228E-5	0.0	6.561050699781164E-5	8.201313374726455E-5
38-39	5.207833992951299E-4	4.100656687363228E-5	0.0	6.561050699781164E-5	8.611379043462779E-5
40-41	5.535886527940357E-4	4.920788024835873E-5	0.0	6.561050699781164E-5	9.841576049671747E-5
42-43	6.109978464171209E-4	4.920788024835873E-5	0.0	6.971116368517487E-5	9.841576049671747E-5
44-45	6.684070400402062E-4	4.920788024835873E-5	0.0	7.791247705990133E-5	1.0251641718408069E-4
46-47	7.094136069138384E-4	4.920788024835873E-5	0.0	8.201313374726455E-5	1.0661707387144393E-4
48-49	7.955273973484662E-4	4.920788024835873E-5	0.0	8.201313374726455E-5	1.0661707387144393E-4
50-51	8.81641187783094E-4	4.920788024835873E-5	0.0	8.201313374726455E-5	1.1891904393353361E-4
52-53	9.677549782177218E-4	4.920788024835873E-5	0.0	8.201313374726455E-5	1.2301970062089684E-4
54-55	0.0010702713954018023	4.920788024835873E-5	0.0	8.201313374726455E-5	1.353216706829865E-4
56-57	0.0011686871558985199	4.920788024835873E-5	0.0	8.201313374726455E-5	1.476236407450762E-4
58-59	0.001238398319583695	4.920788024835873E-5	0.0	8.201313374726455E-5	1.476236407450762E-4
60-61	0.0013368140800804123	4.920788024835873E-5	0.0	9.021444712199102E-5	1.5172429743243944E-4
62-63	0.0014516324673265826	5.740919362308519E-5	0.0	9.431510380935424E-5	1.5582495411980266E-4
64-65	0.0016074574214463852	6.561050699781164E-5	0.0	9.841576049671747E-5	1.5582495411980266E-4
66-67	0.0017386784354420084	6.561050699781164E-5	0.0	9.841576049671747E-5	1.5992561080716588E-4
68-69	0.0019068053596239009	6.561050699781164E-5	0.0	9.841576049671747E-5	1.7222758086925556E-4
70-71	0.0021364421341162417	6.561050699781164E-5	0.0	9.841576049671747E-5	1.7222758086925556E-4
72-73	0.002308669714985497	6.561050699781164E-5	0.0	9.841576049671747E-5	1.7222758086925556E-4
74-75	0.002489098609229479	6.561050699781164E-5	0.0	1.0661707387144393E-4	1.7222758086925556E-4
76-77	0.002776144577344905	6.561050699781164E-5	0.0	1.0661707387144393E-4	1.8042889424398203E-4
78-79	0.0030713918588350575	6.561050699781164E-5	0.0	1.0661707387144393E-4	1.8042889424398203E-4
80-81	0.0033748404536999366	6.561050699781164E-5	0.0	1.0661707387144393E-4	1.8042889424398203E-4
82-83	0.003866919256183524	6.561050699781164E-5	0.0	1.0661707387144393E-4	1.8042889424398203E-4
84-85	0.004650144683469901	7.38118203725381E-5	0.0	1.1071773055880715E-4	1.8042889424398203E-4
86-87	0.005810630525993694	7.38118203725381E-5	0.0	1.1481838724617038E-4	1.8042889424398203E-4
88-89	0.007290967590131819	7.38118203725381E-5	0.0	1.1481838724617038E-4	1.8042889424398203E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGCAGT	415085	0.0	92.23629	1
AGTGGTA	414985	0.0	91.70787	5
AGCAGTG	418560	0.0	91.369095	2
GGTATCA	417800	0.0	91.25191	8
TGGTATC	418120	0.0	91.17753	7
GTATCAA	418095	0.0	91.170105	9
GTGGTAT	418725	0.0	91.10551	6
GCAGTGG	419080	0.0	91.07135	3
CAGTGGT	420690	0.0	90.56135	4
ATCAACG	417825	0.0	46.00003	10-11
CAACGCA	418675	0.0	45.981285	12-13
TCAACGC	418500	0.0	45.969887	12-13
AACGCAG	420050	0.0	45.83162	14-15
ACGCAGA	420440	0.0	45.831444	14-15
TATCAAC	419900	0.0	45.709408	10-11
CGCAGAG	421400	0.0	45.694942	16-17
AGAGTAC	411585	0.0	45.60962	18-19
CAGAGTA	419285	0.0	45.506065	18-19
GAGTACT	230900	0.0	45.385925	20-21
GCAGAGT	423250	0.0	45.353363	16-17
>>END_MODULE