FastQCFastQC Report
Sat 18 Jun 2016
SRR578605_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR578605_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12193169
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3081692.5273905413760773No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT294950.24189773798755682No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT265600.21782688323273464No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT143900.028.3452341
AGTGGTA170350.023.0474935
CGCGTAG3900.021.88084654-55
GGTATCA181150.021.3069618
GTATCAA183550.021.1822349
CGTCACG8400.020.8877563
TGGTATC191400.020.4136567
AGCAGTG201250.020.172132
ATCAACG166200.019.86760110-11
CAACGCA169300.019.43380512-13
GTGGTAT203950.019.2505046
GCAGTGG214500.018.5704023
ACTCGCG5050.017.36743750-51
ACGCAGA190650.017.2699414-15
CTCGCGT5000.017.06702652-53
CAGTGGT234900.016.8353484
AGAGTAC198250.016.1302818-19
TCAACGC165600.015.94594310-11
ACGTACG5750.015.66702520-21
CGTACGC6350.015.30667620-21
Produced by FastQC (version 0.11.5)