##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR578605_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 12193169 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.971121781384316 31.0 31.0 34.0 30.0 34.0 2 32.24376050229436 34.0 31.0 34.0 30.0 34.0 3 32.31764728267114 34.0 31.0 34.0 30.0 34.0 4 35.88463729158515 37.0 35.0 37.0 35.0 37.0 5 35.76093614383595 37.0 35.0 37.0 33.0 37.0 6 35.73387386002769 37.0 35.0 37.0 33.0 37.0 7 35.724297596465696 37.0 35.0 37.0 33.0 37.0 8 35.64209673465528 37.0 35.0 37.0 33.0 37.0 9 37.27162266019605 39.0 37.0 39.0 33.0 39.0 10-11 37.26388242465925 39.0 37.0 39.0 33.0 39.0 12-13 37.26233454977947 39.0 37.0 39.0 33.0 39.0 14-15 38.56608790544935 40.0 38.0 41.0 34.0 41.0 16-17 38.50132024742706 40.0 38.0 41.0 33.0 41.0 18-19 38.38689568724915 40.0 38.0 41.0 33.5 41.0 20-21 38.29197290712529 40.0 37.5 41.0 33.0 41.0 22-23 38.15763477894877 40.0 37.0 41.0 33.0 41.0 24-25 38.06690328002507 40.0 37.0 41.0 33.0 41.0 26-27 37.89244736130534 40.0 37.0 41.0 33.0 41.0 28-29 37.78617810513411 40.0 37.0 41.0 33.0 41.0 30-31 37.677536044977316 40.0 37.0 41.0 32.0 41.0 32-33 37.53390455754365 40.0 36.0 41.0 32.0 41.0 34-35 37.38678894715558 40.0 36.0 41.0 31.0 41.0 36-37 37.240321855622604 39.5 36.0 41.0 31.0 41.0 38-39 37.08460778325963 39.0 35.5 41.0 31.0 41.0 40-41 36.91879662292879 39.0 35.0 41.0 30.5 41.0 42-43 36.73901485331664 39.0 35.0 41.0 30.0 41.0 44-45 36.563870393332536 39.0 35.0 40.0 30.0 41.0 46-47 36.62933709030031 39.0 35.0 41.0 30.0 41.0 48-49 36.58767827297399 39.0 35.0 41.0 30.0 41.0 50-51 36.53190237090948 39.0 35.0 41.0 30.0 41.0 52-53 36.50785029716229 39.0 35.0 41.0 30.0 41.0 54-55 36.35175269037934 39.0 35.0 41.0 30.0 41.0 56-57 36.18420047323218 38.5 35.0 41.0 30.0 41.0 58-59 35.956412151754805 38.0 35.0 40.0 29.0 41.0 60-61 35.71864648148484 38.0 34.5 40.0 29.0 41.0 62-63 35.43804760681985 37.0 34.0 40.0 28.0 41.0 64-65 35.123313061600314 37.0 34.0 40.0 28.0 41.0 66-67 34.804963869523995 36.0 34.0 39.5 28.0 41.0 68-69 34.476425242691214 36.0 34.0 39.0 28.0 41.0 70-71 34.118029611498045 35.0 33.0 39.0 27.0 40.5 72-73 33.70802069585028 35.0 33.0 38.0 26.0 40.0 74-75 33.34109918430558 35.0 33.0 37.0 26.0 39.5 76-77 31.92688127262076 34.0 30.5 36.0 24.5 39.0 78-79 32.54846106865246 35.0 32.0 36.0 26.0 39.0 80-81 32.51511231411621 35.0 33.0 36.0 26.0 38.0 82-83 32.25755601353512 35.0 32.0 35.5 26.0 37.0 84-85 31.99002388140442 35.0 32.0 35.0 25.0 37.0 86-87 31.72278166570151 35.0 32.0 35.0 25.0 36.0 88-89 31.479197532651273 35.0 32.0 35.0 24.5 36.0 90-91 31.242154972181556 35.0 32.0 35.0 24.0 36.0 92-93 30.99889401188485 35.0 32.0 35.0 23.0 35.5 94-95 30.739764166313122 34.0 31.0 35.0 20.5 35.0 96-97 30.131569118741815 34.0 31.0 35.0 11.5 35.0 98-99 30.08205807694456 34.0 31.0 35.0 9.5 35.0 100-101 29.477229709520145 34.0 30.5 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.44515226828015386 1101 2 0.41641227970880124 1101 3 0.3970465953606279 1101 4 0.2855773101430543 1101 5 0.2718486647797391 1101 6 0.2789295032533019 1101 7 0.32042869987283495 1101 8 0.364030997462514 1101 9 0.452066767310626 1101 10-11 0.4113089052203662 1101 12-13 0.4399713014403446 1101 14-15 0.5642414190784137 1101 16-17 0.5516397885458133 1101 18-19 0.5609040743497005 1101 20-21 0.6368020819212177 1101 22-23 0.670138881108393 1101 24-25 0.6875270710840198 1101 26-27 0.7065772763652944 1101 28-29 0.7075856185149334 1101 30-31 0.6640173170181995 1101 32-33 0.6436319534305568 1101 34-35 0.690263045783837 1101 36-37 0.6895493300016611 1101 38-39 0.7153544249467103 1101 40-41 0.7373943409721448 1101 42-43 0.7360883765668902 1101 44-45 0.7172628341603655 1101 46-47 0.7732314600046877 1101 48-49 0.7240834224542354 1101 50-51 0.6616386778934995 1101 52-53 0.622079909052303 1101 54-55 0.705092044531149 1101 56-57 0.7205089320409002 1101 58-59 0.7127371594058829 1101 60-61 0.6571742544578214 1101 62-63 0.7073809718039001 1101 64-65 0.6897761574749453 1101 66-67 0.6578311350118753 1101 68-69 0.6858422120122825 1101 70-71 0.6599895097753148 1101 72-73 0.622225533719913 1101 74-75 0.6299823130187789 1101 76-77 0.6247326156731461 1101 78-79 0.5657922007915133 1101 80-81 0.49367967242715594 1101 82-83 0.49334980340542955 1101 84-85 0.41417692702187026 1101 86-87 0.4389395568594665 1101 88-89 0.46729048953232777 1101 90-91 0.4711993386278017 1101 92-93 0.5415148396523755 1101 94-95 0.5417420269363014 1101 96-97 0.8710381144524852 1101 98-99 0.6565149335460809 1101 100-101 0.61135183384323 1102 1 0.37916547590754135 1102 2 0.3567802527977548 1102 3 0.3513945984708471 1102 4 0.2509758197450296 1102 5 0.303493752310537 1102 6 0.2641094759127611 1102 7 0.2330664204473507 1102 8 0.2807598541250229 1102 9 0.44016347239384146 1102 10-11 0.3607412143284279 1102 12-13 0.37931562575649025 1102 14-15 0.45247163808376456 1102 16-17 0.5069463859459375 1102 18-19 0.5383494995656903 1102 20-21 0.5274047087459337 1102 22-23 0.5409333276786796 1102 24-25 0.5953015952368688 1102 26-27 0.6304497854325604 1102 28-29 0.6505210547781104 1102 30-31 0.6758353979487808 1102 32-33 0.7002238227551771 1102 34-35 0.7335610648559125 1102 36-37 0.7401656270954504 1102 38-39 0.7663287108607122 1102 40-41 0.7630593523616369 1102 42-43 0.7782810999770646 1102 44-45 0.7860786227806784 1102 46-47 0.84239767087562 1102 48-49 0.8435601869602465 1102 50-51 0.8218186145216819 1102 52-53 0.7560939093679409 1102 54-55 0.8058274250179025 1102 56-57 0.7327713867946173 1102 58-59 0.8262551963062421 1102 60-61 0.8212160060682336 1102 62-63 0.7505485035816903 1102 64-65 0.7448074398355473 1102 66-67 0.7911582169174665 1102 68-69 0.8091193941929404 1102 70-71 0.8021078826313612 1102 72-73 0.861393250698498 1102 74-75 0.7330305776440653 1102 76-77 0.7067376196978472 1102 78-79 0.7212263309206506 1102 80-81 0.7379487706289822 1102 82-83 0.7365577983149336 1102 84-85 0.7446897674904775 1102 86-87 0.6957312016371304 1102 88-89 0.6676244815175245 1102 90-91 0.6755961100806509 1102 92-93 0.7020813198931783 1102 94-95 0.7315663059683253 1102 96-97 1.1547720616147714 1102 98-99 0.9234778854594516 1102 100-101 0.921931393440051 1103 1 0.3385414463273051 1103 2 0.29846399002519775 1103 3 0.2787560531145985 1103 4 0.23133087116750062 1103 5 0.21062401582759804 1103 6 0.280852625117781 1103 7 0.273340215329668 1103 8 0.07143958215839064 1103 9 0.18008106375192767 1103 10-11 0.37017733731215685 1103 12-13 0.3526724551865712 1103 14-15 0.4931881332751118 1103 16-17 0.4741447159739067 1103 18-19 0.40405689763725405 1103 20-21 0.40799430803166103 1103 22-23 0.45548971589227705 1103 24-25 0.24589423207437733 1103 26-27 0.26528085546675584 1103 28-29 0.48818271578684147 1103 30-31 0.28887658962946006 1103 32-33 0.4838546317286898 1103 34-35 0.47079001332102166 1103 36-37 0.5328557682856427 1103 38-39 0.6006654257874828 1103 40-41 0.447469394169552 1103 42-43 0.5186111600067633 1103 44-45 0.6571891424230571 1103 46-47 0.5118593662461421 1103 48-49 0.5340470527000747 1103 50-51 0.4812449748288472 1103 52-53 0.5936222551139991 1103 54-55 0.6645311795455839 1103 56-57 0.5744455740765062 1103 58-59 0.288031546732455 1103 60-61 0.2960906527714471 1103 62-63 0.16006760048926338 1103 64-65 0.4118481405930581 1103 66-67 0.41038752616026386 1103 68-69 0.3561271188180868 1103 70-71 0.37488237634831023 1103 72-73 0.5521309986894636 1103 74-75 0.4709168181700676 1103 76-77 0.30004207951800055 1103 78-79 0.42210782911617883 1103 80-81 0.4476440803178505 1103 82-83 0.4858599593690158 1103 84-85 0.45014240948790274 1103 86-87 0.3416701197298977 1103 88-89 0.32513040064775467 1103 90-91 0.4134031369904321 1103 92-93 0.408902033298741 1103 94-95 0.42585728249055776 1103 96-97 0.5015347201077631 1103 98-99 0.2823079457186459 1103 100-101 0.5600311461742287 1104 1 0.3271665037078577 1104 2 0.3042079607342245 1104 3 0.27714906431675246 1104 4 0.18602679298169278 1104 5 0.21565475600649364 1104 6 0.22322650061438765 1104 7 0.2279362132889986 1104 8 0.3204919454310087 1104 9 0.286772878449149 1104 10-11 0.322922406959151 1104 12-13 0.3246160011636121 1104 14-15 0.3650855354123266 1104 16-17 0.4332925634693794 1104 18-19 0.4347131971079179 1104 20-21 0.37000477588942715 1104 22-23 0.5036425871347916 1104 24-25 0.4664490073442664 1104 26-27 0.5524925653265242 1104 28-29 0.544408969482923 1104 30-31 0.556149351179279 1104 32-33 0.6049119381257668 1104 34-35 0.5663146290823491 1104 36-37 0.5988401202881874 1104 38-39 0.6072702837775665 1104 40-41 0.5698455874847284 1104 42-43 0.6621809598016242 1104 44-45 0.5996576888436138 1104 46-47 0.47043935567281636 1104 48-49 0.6049100963777079 1104 50-51 0.463133051827775 1104 52-53 0.5381604189075944 1104 54-55 0.6045632841603137 1104 56-57 0.6111875228007406 1104 58-59 0.6245477974440163 1104 60-61 0.5551982275699103 1104 62-63 0.3566372645393514 1104 64-65 0.5454005280313652 1104 66-67 0.5868295640012064 1104 68-69 0.6713093621277082 1104 70-71 0.6011091803822097 1104 72-73 0.7131080865831123 1104 74-75 0.6296337320121737 1104 76-77 0.5129896127816025 1104 78-79 0.5241808935816792 1104 80-81 0.6913982298484811 1104 82-83 0.7176131978225939 1104 84-85 0.7338022726237412 1104 86-87 0.7068712684900333 1104 88-89 0.6341649354779655 1104 90-91 0.6845002141699013 1104 92-93 0.6821656431088492 1104 94-95 0.7326689544659715 1104 96-97 1.0566625374083145 1104 98-99 0.8047358243374525 1104 100-101 0.8091691953850741 1105 1 0.15094902400534238 1105 2 0.13298196029327158 1105 3 0.11116343166257536 1105 4 0.0420913411978745 1105 5 0.10700063775375668 1105 6 0.06660618659434903 1105 7 0.05224314682325826 1105 8 0.2035133086468761 1105 9 0.1913729975519658 1105 10-11 0.13929887770281368 1105 12-13 0.1439172494702632 1105 14-15 0.16487764175364816 1105 16-17 0.14364566554095148 1105 18-19 0.11043137261864899 1105 20-21 0.15971064633443177 1105 22-23 0.1850336843411 1105 24-25 0.18791405157595875 1105 26-27 0.13728135315417234 1105 28-29 0.1845258220772692 1105 30-31 0.1412026363373471 1105 32-33 0.18372184326400287 1105 34-35 0.1494908799460717 1105 36-37 0.18255609131711026 1105 38-39 0.19233044107170372 1105 40-41 0.21008580113676345 1105 42-43 0.15823535927565757 1105 44-45 0.2572749242887511 1105 46-47 0.22681566801995956 1105 48-49 0.2105633818767103 1105 50-51 0.18220035983698324 1105 52-53 0.25810776875621855 1105 54-55 0.3128075283290528 1105 56-57 0.2924302564491015 1105 58-59 0.2684796089490291 1105 60-61 0.273393520110524 1105 62-63 0.2767102377457604 1105 64-65 0.26282718902132984 1105 66-67 0.27243970882250323 1105 68-69 0.2323278219282372 1105 70-71 0.27063611176852476 1105 72-73 0.31539530394121584 1105 74-75 0.32066664922434285 1105 76-77 0.3343150514334212 1105 78-79 0.3117262851947018 1105 80-81 0.28487993242214316 1105 82-83 0.2871777537100044 1105 84-85 0.3431854943265975 1105 86-87 0.26880849914491733 1105 88-89 0.22096505725150806 1105 90-91 0.2770310936783069 1105 92-93 0.27160239218148163 1105 94-95 0.36980028123501185 1105 96-97 0.7362120086906678 1105 98-99 0.544469563336353 1105 100-101 0.5170123873747272 1106 1 -0.0517332527918235 1106 2 -0.08371439717995344 1106 3 -0.06576939646978985 1106 4 -0.04157369071712225 1106 5 -0.029580945515817803 1106 6 -0.04526901993301635 1106 7 -0.0302812680730824 1106 8 -0.060459128612997404 1106 9 -0.014636085984669478 1106 10-11 -0.0647087138175948 1106 12-13 -0.056721464322919246 1106 14-15 -0.13210032659588222 1106 16-17 -0.13472746527472879 1106 18-19 -0.13495112746316096 1106 20-21 -0.1514118017419861 1106 22-23 -0.17211661657761823 1106 24-25 -0.06473633984100502 1106 26-27 -0.0810263570918579 1106 28-29 -0.10397816656915637 1106 30-31 -0.2142103442213923 1106 32-33 -0.30272064317877323 1106 34-35 -0.3591928992935962 1106 36-37 -0.26337294635047215 1106 38-39 -0.2886134908392961 1106 40-41 -0.3142156623676229 1106 42-43 -0.2600635053809981 1106 44-45 -0.21493728159136793 1106 46-47 -0.23967174359056287 1106 48-49 -0.2222750915548204 1106 50-51 -0.2376038080827172 1106 52-53 -0.15166110137994337 1106 54-55 -0.18028512898015236 1106 56-57 -0.1647715637610787 1106 58-59 -0.11620318764589399 1106 60-61 -0.22102008804689888 1106 62-63 -0.13206003085218754 1106 64-65 -0.11428257705239986 1106 66-67 -0.12741861305249813 1106 68-69 -0.11751250197438168 1106 70-71 -0.14394594795979287 1106 72-73 -0.09820660006947435 1106 74-75 -0.10895660924569484 1106 76-77 -0.14310865563004072 1106 78-79 -0.10671486676627495 1106 80-81 -0.15797429686098496 1106 82-83 -0.13889719830323344 1106 84-85 -0.143216193557965 1106 86-87 -0.16374886139163536 1106 88-89 -0.22554088469476952 1106 90-91 -0.17012039027664727 1106 92-93 -0.14880244036137924 1106 94-95 -0.0950742686129118 1106 96-97 0.18434064066891764 1106 98-99 -0.052580569400678456 1106 100-101 -0.05423544826080118 1107 1 -0.16845182005725334 1107 2 -0.23918159264320593 1107 3 -0.19161444810439576 1107 4 -0.17226362557403974 1107 5 -0.16180451290468767 1107 6 -0.13856503541981624 1107 7 -0.14600409232917144 1107 8 -0.13926875012745654 1107 9 -0.22047671932602242 1107 10-11 -0.24106961054189924 1107 12-13 -0.20218309158160963 1107 14-15 -0.2720251796245208 1107 16-17 -0.29660670417804624 1107 18-19 -0.3075262860561452 1107 20-21 -0.27890967312633563 1107 22-23 -0.23584644291081958 1107 24-25 -0.157098574686664 1107 26-27 -0.1935159869165659 1107 28-29 -0.1597846085619139 1107 30-31 -0.2300957294897472 1107 32-33 -0.30175929332401097 1107 34-35 -0.304241019521136 1107 36-37 -0.3293520398736405 1107 38-39 -0.33950424387347056 1107 40-41 -0.32231740984012447 1107 42-43 -0.38957277813642577 1107 44-45 -0.26161522799132086 1107 46-47 -0.2989441897459102 1107 48-49 -0.39430949623804423 1107 50-51 -0.36296125351993425 1107 52-53 -0.3819561631721129 1107 54-55 -0.33907054537038306 1107 56-57 -0.3266880902856144 1107 58-59 -0.2869439024676481 1107 60-61 -0.3913466343623142 1107 62-63 -0.5211497229271984 1107 64-65 -0.48617793823058975 1107 66-67 -0.4150974534912635 1107 68-69 -0.4249912798325042 1107 70-71 -0.3695279608029054 1107 72-73 -0.35067931543510156 1107 74-75 -0.36391958315191886 1107 76-77 -0.4227618196093985 1107 78-79 -0.2634608203620772 1107 80-81 -0.30439067016062893 1107 82-83 -0.2990213485810784 1107 84-85 -0.28980621981604315 1107 86-87 -0.29970797109056235 1107 88-89 -0.31506383781792735 1107 90-91 -0.2503089592132923 1107 92-93 -0.2510088040906062 1107 94-95 -0.2829703572989537 1107 96-97 -0.047851458931841506 1107 98-99 -0.2909641737722133 1107 100-101 -0.36227186008778034 1108 1 -0.34994388507426066 1108 2 -0.3392273294348982 1108 3 -0.32541626710143134 1108 4 -0.23209511945014327 1108 5 -0.26283623708281567 1108 6 -0.24488102019162028 1108 7 -0.23278945240712545 1108 8 -0.22578236299580823 1108 9 -0.30504766190755817 1108 10-11 -0.38101355092485534 1108 12-13 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-0.1122800698802422 2305 18-19 -0.14878614680789326 2305 20-21 -0.13298825383789392 2305 22-23 -0.14326148585927712 2305 24-25 -0.06911659655148128 2305 26-27 -0.16075243244138449 2305 28-29 -0.16632006126972954 2305 30-31 -0.17337130884305196 2305 32-33 -0.19860251337835422 2305 34-35 -0.17409523315598818 2305 36-37 -0.14588903075465254 2305 38-39 -0.18939470378013823 2305 40-41 -0.1501299627634154 2305 42-43 -0.22362089583584321 2305 44-45 -0.20038255999125454 2305 46-47 -0.12276987476813161 2305 48-49 -0.16454042790248025 2305 50-51 -0.1822017277818233 2305 52-53 -0.1777545201188886 2305 54-55 -0.09419786144856346 2305 56-57 -0.18665060366456032 2305 58-59 -0.11849425294349203 2305 60-61 -0.14120980915901526 2305 62-63 -0.12078327544474377 2305 64-65 -0.08892501997452484 2305 66-67 -0.13315831846163206 2305 68-69 -0.10422064049740953 2305 70-71 -0.10785348709699605 2305 72-73 -0.1272462056526038 2305 74-75 -0.18291511909912828 2305 76-77 -0.203446428659106 2305 78-79 -0.1559091783156603 2305 80-81 -0.13667518819664082 2305 82-83 -0.14922007819323113 2305 84-85 -0.12237881273840756 2305 86-87 -0.05209831912162599 2305 88-89 -0.08106708161366427 2305 90-91 -0.07940455311900863 2305 92-93 -0.07497721194240015 2305 94-95 -0.14652645628105532 2305 96-97 0.1539257615245866 2305 98-99 -0.11455175975533649 2305 100-101 -0.1102168268063366 2306 1 -0.24033168547737915 2306 2 -0.2776079837608343 2306 3 -0.257059469054397 2306 4 -0.17887905762723477 2306 5 -0.22550153375167525 2306 6 -0.16938962011013814 2306 7 -0.20202297525240454 2306 8 -0.16683807879203982 2306 9 -0.3488048012158771 2306 10-11 -0.2485395655728979 2306 12-13 -0.2961996504807658 2306 14-15 -0.4135306536847949 2306 16-17 -0.4460056870065756 2306 18-19 -0.3993779543596645 2306 20-21 -0.4679559079594213 2306 22-23 -0.42188618106698783 2306 24-25 -0.4150999335174106 2306 26-27 -0.4652205948139354 2306 28-29 -0.5491229606828867 2306 30-31 -0.5473123007891232 2306 32-33 -0.6081813212880007 2306 34-35 -0.5798164023418124 2306 36-37 -0.615098308818034 2306 38-39 -0.7836325948309906 2306 40-41 -0.6523197651324679 2306 42-43 -0.7085883732878528 2306 44-45 -0.7026206026517272 2306 46-47 -0.6501731380824438 2306 48-49 -0.6144644178808036 2306 50-51 -0.5828313154305462 2306 52-53 -0.5056222734172451 2306 54-55 -0.5470514098381685 2306 56-57 -0.5586963285081978 2306 58-59 -0.6009213953175703 2306 60-61 -0.5654377640443684 2306 62-63 -0.5046680962688299 2306 64-65 -0.5635369021846586 2306 66-67 -0.5362220057300533 2306 68-69 -0.5713103069912933 2306 70-71 -0.6681147679961228 2306 72-73 -0.6971474357046077 2306 74-75 -0.6375224032812028 2306 76-77 -0.6493846917949782 2306 78-79 -0.5272717615436449 2306 80-81 -0.5436873535781288 2306 82-83 -0.46583482168852797 2306 84-85 -0.45355750501378367 2306 86-87 -0.5014723776599581 2306 88-89 -0.5006318214654861 2306 90-91 -0.5437295713248069 2306 92-93 -0.562381020486022 2306 94-95 -0.5690213565795155 2306 96-97 -0.28754637093819113 2306 98-99 -0.5767291139901403 2306 100-101 -0.5709667103607394 2307 1 -0.4710084926483269 2307 2 -0.4534146134040036 2307 3 -0.47666733815014695 2307 4 -0.3890324477033076 2307 5 -0.3947077780455288 2307 6 -0.36486902222461026 2307 7 -0.41671884038444773 2307 8 -0.3949981839348453 2307 9 -0.47256413645438755 2307 10-11 -0.5762280538620814 2307 12-13 -0.5572595116815293 2307 14-15 -0.8833271926899471 2307 16-17 -0.7579800784712347 2307 18-19 -0.7102745642091222 2307 20-21 -0.6075919625292272 2307 22-23 -0.6267830927618263 2307 24-25 -0.60375467618843 2307 26-27 -0.6742114995706316 2307 28-29 -0.6799206301188434 2307 30-31 -0.727498435462131 2307 32-33 -0.7600611775606225 2307 34-35 -0.7630839910821194 2307 36-37 -0.8159498305142563 2307 38-39 -0.8727383845198915 2307 40-41 -0.7335831270445752 2307 42-43 -0.8802790168589425 2307 44-45 -0.7745672024975931 2307 46-47 -0.7999953488383724 2307 48-49 -0.7065532805641581 2307 50-51 -0.7913079577449338 2307 52-53 -0.942065593259656 2307 54-55 -0.9276457639064333 2307 56-57 -0.9467217301867805 2307 58-59 -0.9014756307592862 2307 60-61 -1.0342693990285738 2307 62-63 -0.9731780110785948 2307 64-65 -0.9347080953460107 2307 66-67 -0.956085806277784 2307 68-69 -0.8867974488581254 2307 70-71 -0.9730179350392376 2307 72-73 -1.331365953223468 2307 74-75 -1.0371812220232144 2307 76-77 -0.9395236813311705 2307 78-79 -0.8813134612177578 2307 80-81 -0.8515549185878228 2307 82-83 -0.8494841438851353 2307 84-85 -0.8989104597308497 2307 86-87 -0.8419547080386565 2307 88-89 -0.9310589856298215 2307 90-91 -1.025981079682893 2307 92-93 -0.9737838667356833 2307 94-95 -1.0160659361961173 2307 96-97 -0.8075809110517724 2307 98-99 -1.180059846996155 2307 100-101 -1.2081610608876225 2308 1 -1.3647532847825765 2308 2 -0.8301397018305607 2308 3 -0.8560990666754087 2308 4 -0.521998950304102 2308 5 -0.6003053637752984 2308 6 -0.6116158932235791 2308 7 -0.57738496139293 2308 8 -0.6023057609632616 2308 9 -0.822959850272106 2308 10-11 -0.7966921608362441 2308 12-13 -0.7538442707982398 2308 14-15 -0.9462184781888041 2308 16-17 -0.8788629485970318 2308 18-19 -0.8803549842941223 2308 20-21 -0.9394648110591106 2308 22-23 -0.9971215036703498 2308 24-25 -1.1399873950804746 2308 26-27 -1.1391266410151815 2308 28-29 -1.2021553595031875 2308 30-31 -1.0711106043916416 2308 32-33 -1.1453265969871325 2308 34-35 -1.2025890317808958 2308 36-37 -1.2186472753672888 2308 38-39 -1.2570473569650105 2308 40-41 -1.192939822760259 2308 42-43 -1.1379413276927508 2308 44-45 -1.1256278768453498 2308 46-47 -1.1709406741965793 2308 48-49 -1.1970376175219428 2308 50-51 -1.2413197891121186 2308 52-53 -1.1965172924360843 2308 54-55 -1.1574854603304772 2308 56-57 -1.0294999204948496 2308 58-59 -1.0106135592834562 2308 60-61 -0.9764168750506954 2308 62-63 -1.030637395950599 2308 64-65 -0.9986632466602785 2308 66-67 -0.9876143210806418 2308 68-69 -1.0850861336426263 2308 70-71 -1.0616522466108336 2308 72-73 -1.0799798426181155 2308 74-75 -1.1873870787464185 2308 76-77 -1.162300949962166 2308 78-79 -1.5514249862578566 2308 80-81 -1.2449190719690932 2308 82-83 -1.1990672942466247 2308 84-85 -1.1803921357208864 2308 86-87 -1.1375894766743073 2308 88-89 -1.1512418007934428 2308 90-91 -1.1940017347304313 2308 92-93 -1.106588720052745 2308 94-95 -1.2169112194922427 2308 96-97 -1.0191971118702448 2308 98-99 -1.279915450921706 2308 100-101 -1.3236435455836961 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 69.0 3 159.0 4 370.0 5 1108.0 6 2079.0 7 3757.0 8 6247.0 9 9154.0 10 12339.0 11 15040.0 12 18010.0 13 20486.0 14 22563.0 15 25170.0 16 28861.0 17 34238.0 18 39932.0 19 46096.0 20 48772.0 21 49917.0 22 54004.0 23 63021.0 24 77271.0 25 96031.0 26 120960.0 27 153745.0 28 193447.0 29 240931.0 30 296583.0 31 364020.0 32 449694.0 33 565439.0 34 756929.0 35 1196703.0 36 1509299.0 37 2386009.0 38 2598151.0 39 684377.0 40 2188.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 17.51089475173582 30.565157300283392 28.16850057211288 23.75544737586791 2 23.078821710126753 30.02249372403608 26.277061454409317 20.621623111427848 3 22.619797594595546 28.34457292912399 26.465739771486657 22.56988970479381 4 22.218003082396027 28.15200090018036 27.042267798409465 22.587728219014146 5 22.762903619604938 28.434961134893744 27.528339661107527 21.273795584393792 6 22.887397033535745 28.37299310786228 26.26695324242615 22.47265661617583 7 23.05408872787706 28.398630413471675 27.158887078494526 21.388393780156743 8 22.39264286739527 28.736377616928788 26.723139699399205 22.147839816276733 9 21.87491977236308 28.44541252117907 27.47860439140145 22.201063315056402 10-11 21.867338179270703 29.01057141092689 26.74047657339942 22.381613836402988 12-13 21.858685793660367 29.10375063283384 26.515801593498782 22.521761980007003 14-15 22.12651198388212 29.182520147141407 26.334962633586066 22.356005235390406 16-17 22.360401139359258 28.833562464360167 26.392863906011637 22.413172490268938 18-19 22.22097471133222 29.01816992776857 26.398338282689267 22.36251707820994 20-21 22.350198705521098 28.88113418259027 26.527480263744398 22.241186848144235 22-23 22.244379459904415 28.764192438602755 26.669263205142986 22.32216489634985 24-25 22.191985729691442 28.74654549360048 26.577609012229026 22.483859764479053 26-27 22.401164896482445 28.569978596376345 26.846780204732024 22.182076302409186 28-29 22.413672770384796 28.643976803733302 26.74328552323026 22.199064902651642 30-31 22.322059999332414 28.675892214731054 26.707134133874465 22.294913652062068 32-33 22.349440084033937 28.728843994534977 26.62783153419755 22.29388438723354 34-35 22.349025917708516 28.749416989135472 26.63022631770297 22.271330775453045 36-37 22.37253088184048 28.74220803467909 26.589006516681597 22.296254566798837 38-39 22.38334021286837 28.72825760062868 26.583794582031956 22.304607604470995 40-41 22.3411772608089 28.776198377960643 26.571066143674383 22.311558217556076 42-43 22.383951210714788 28.78128319225297 26.515420232426862 22.31934536460538 44-45 22.398742279386106 28.801610147452234 26.516191155884083 22.283456417277574 46-47 22.378570719157707 28.860229667977155 26.47054288684802 22.290656726017122 48-49 22.4308381192781 28.837818946001654 26.437561063903896 22.293781870816357 50-51 22.44801987079815 28.81442880025693 26.433292280292353 22.30425904865257 52-53 22.407595597174122 28.897704936263903 26.392507148879833 22.302192317682138 54-55 22.446268890392645 28.899197575298103 26.296211427890487 22.358322106418765 56-57 22.448913813955997 28.97842226249796 26.275769654303982 22.296894269242067 58-59 22.43616897297167 29.045648428230592 26.2175485306568 22.30063406814094 60-61 22.413404530621715 29.029502685972897 26.210971871855048 22.346120911550337 62-63 22.398945736098426 29.069501470989618 26.242246467525376 22.289306325386576 64-65 22.40151081816287 29.095498337444738 26.255948176056705 22.247042668335695 66-67 22.36580024084857 29.124806545911046 26.244030607870155 22.265362605370225 68-69 22.3797978270376 29.144758144016254 26.254186375862332 22.22125765308382 70-71 22.391230553737824 29.196701624264044 26.156524075438075 22.255543746560058 72-73 22.356680543022346 29.18908325920026 26.169149055089314 22.285087142688077 74-75 22.413403611524377 29.20433720163552 26.146032757788046 22.236226429052056 76-77 22.363999096240345 29.30930911450153 26.11536123308751 22.211330556170623 78-79 22.419270649342806 29.32248241055686 26.05792476204473 22.200322178055607 80-81 22.37036271580243 29.351324575140726 26.073059023689932 22.205253685366912 82-83 22.394443221965453 29.43567207552793 26.002610701168294 22.16727400133832 84-85 22.370327409235216 29.432841383679552 26.02947875712215 22.167352449963083 86-87 22.373419089577386 29.48674878080753 25.97442100597178 22.1654111236433 88-89 22.370596493697516 29.537850823544893 25.952932279200912 22.138620403556686 90-91 22.33412009406961 29.519272681218002 25.981810295469355 22.164796929243032 92-93 22.353927295503308 29.585684438836818 25.925766528427058 22.13462173723282 94-95 22.303391513606833 29.639823819117083 25.92624629041076 22.130538376865324 96-97 22.29447972707784 29.660638466342487 25.898320014395786 22.146561792183896 98-99 22.29612567060752 29.66940429246264 25.884450933874703 22.150019103055136 100-101 22.33622215256378 29.74147448575799 25.810298139456194 22.112005222222038 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 330193.0 1 181829.5 2 24041.0 3 13018.5 4 10729.0 5 9450.0 6 9150.5 7 9334.5 8 8684.5 9 8112.5 10 8524.5 11 10766.5 12 23152.0 13 22000.0 14 9921.0 15 9832.5 16 10605.0 17 12198.0 18 14929.5 19 16683.5 20 19248.0 21 24500.5 22 30905.5 23 38639.0 24 48063.0 25 58709.0 26 71772.0 27 86367.5 28 97694.0 29 109126.5 30 125499.0 31 145817.0 32 165362.0 33 183880.5 34 204498.0 35 222962.5 36 242348.0 37 264047.5 38 281296.5 39 292590.5 40 310985.5 41 334265.0 42 352728.0 43 371322.0 44 385599.5 45 401802.0 46 415558.0 47 424669.5 48 440336.5 49 453405.5 50 453741.0 51 450638.0 52 452154.5 53 445365.5 54 422828.0 55 393552.0 56 356485.5 57 318031.5 58 295404.0 59 271465.0 60 232153.5 61 191986.0 62 153801.0 63 117709.0 64 91994.5 65 71557.5 66 55560.5 67 46363.5 68 37006.0 69 28901.0 70 23544.0 71 20715.5 72 19159.0 73 15677.5 74 12055.5 75 9411.5 76 7022.5 77 4817.0 78 3227.5 79 2153.5 80 1456.5 81 1204.0 82 1019.0 83 814.5 84 648.5 85 499.0 86 358.0 87 235.0 88 130.0 89 85.5 90 63.5 91 41.5 92 30.0 93 22.5 94 10.5 95 6.0 96 2.5 97 1.5 98 2.0 99 1.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.012457795016209484 2 0.018149506498269647 3 0.00580652986930633 4 4.6747486235940795E-4 5 8.037287107231926E-4 6 0.0 7 0.0 8 0.007537006991373613 9 0.01064530476039494 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 1.0251641718408069E-4 24-25 0.004760862414028707 26-27 0.006897304548144949 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 1.640262674945291E-5 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 6.561050699781164E-5 62-63 1.3942232737034974E-4 64-65 3.3215319167642144E-4 66-67 2.501400579291569E-4 68-69 3.6905910186269046E-4 70-71 1.312210139956233E-4 72-73 4.920788024835873E-5 74-75 6.150985031044842E-5 76-77 0.12427040091054262 78-79 0.05527685214565631 80-81 1.1481838724617038E-4 82-83 0.002263562491424502 84-85 0.010423869299277324 86-87 0.11734849242227348 88-89 0.1334148653233626 90-91 0.14704954880884533 92-93 0.14508943491228574 94-95 0.11280496481267503 96-97 1.2136016485952095 98-99 0.07589495396971863 100-101 0.0682144239942873 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.2193169E7 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.902261549926266 #Duplication Level Percentage of deduplicated Percentage of total 1 89.2156986759647 54.334378151230126 2 5.878899708416705 7.160765753355588 3 1.5114171211250174 2.761461624653772 4 0.8152437706281315 1.9860075738297023 5 0.5375294379116834 1.6368379209241095 6 0.36849418975444465 1.3465277714432007 7 0.2461614709890347 1.0494253202782144 8 0.17824865026356482 0.8684596735418364 9 0.1423813999051408 0.7804214331180778 >10 1.0284476625172987 12.607513420431285 >50 0.071264388333507 2.8380597933386436 >100 0.0054318628459627954 0.518640568990242 >500 2.9312300452663076E-4 0.12291001397664217 >1k 1.9541533608826296E-4 0.2071707640913858 >5k 4.885383402206574E-5 0.2554600287934279 >10k+ 2.442691701103287E-4 11.525960188003689 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 308169 2.5273905413760773 No Hit AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 29495 0.24189773798755682 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 26560 0.21782688323273464 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 1.640262674945291E-5 0.0 0.0 0.0 0.0 2 2.4603940124179366E-5 0.0 0.0 0.0 1.640262674945291E-5 3 3.280525349890582E-5 8.201313374726455E-6 0.0 0.0 1.640262674945291E-5 4 4.100656687363228E-5 8.201313374726455E-6 0.0 0.0 1.640262674945291E-5 5 4.100656687363228E-5 8.201313374726455E-6 0.0 0.0 2.4603940124179366E-5 6 7.38118203725381E-5 8.201313374726455E-6 0.0 0.0 2.4603940124179366E-5 7 8.201313374726455E-5 8.201313374726455E-6 0.0 0.0 3.280525349890582E-5 8 8.201313374726455E-5 8.201313374726455E-6 0.0 0.0 4.100656687363228E-5 9 9.021444712199102E-5 8.201313374726455E-6 0.0 0.0 4.100656687363228E-5 10-11 1.1481838724617039E-4 8.201313374726455E-6 0.0 0.0 7.381182037253809E-5 12-13 1.4352298405771296E-4 8.201313374726455E-6 0.0 0.0 8.611379043462779E-5 14-15 1.8042889424398203E-4 8.201313374726455E-6 0.0 0.0 9.021444712199102E-5 16-17 2.1323414774288783E-4 8.201313374726455E-6 0.0 8.201313374726455E-6 9.841576049671747E-5 18-19 2.3783808786706723E-4 8.201313374726455E-6 0.0 1.2301970062089683E-5 1.353216706829865E-4 20-21 2.501400579291569E-4 8.201313374726455E-6 0.0 2.050328343681614E-5 1.476236407450762E-4 22-23 2.7884465474069947E-4 8.201313374726455E-6 0.0 2.4603940124179366E-5 1.476236407450762E-4 24-25 3.280525349890582E-4 8.201313374726455E-6 0.0 2.8704596811542594E-5 1.5992561080716588E-4 26-27 3.9366304198686986E-4 8.201313374726455E-6 0.0 3.6905910186269046E-5 1.640262674945291E-4 28-29 4.2646829548577566E-4 8.201313374726455E-6 0.0 4.100656687363228E-5 1.640262674945291E-4 30-31 4.592735489846815E-4 1.2301970062089683E-5 0.0 4.100656687363228E-5 1.763282375566188E-4 32-33 5.002801158583138E-4 2.4603940124179366E-5 0.0 4.920788024835873E-5 1.8042889424398203E-4 34-35 5.863939062929416E-4 2.4603940124179366E-5 0.0 4.920788024835873E-5 1.927308643060717E-4 36-37 6.561050699781164E-4 2.4603940124179366E-5 0.0 5.330853693572196E-5 1.9683152099343493E-4 38-39 7.135142636012016E-4 2.4603940124179366E-5 0.0 6.561050699781164E-5 1.9683152099343493E-4 40-41 7.668228005369235E-4 2.4603940124179366E-5 0.0 6.561050699781164E-5 1.9683152099343493E-4 42-43 8.44735277596825E-4 2.4603940124179366E-5 0.0 7.791247705990133E-5 2.0503283436816138E-4 44-45 8.775405310957308E-4 2.4603940124179366E-5 0.0 8.201313374726455E-5 2.1323414774288785E-4 46-47 9.226477546567262E-4 2.4603940124179366E-5 0.0 8.201313374726455E-5 2.2963677449234075E-4 48-49 0.0010005602317166276 2.4603940124179366E-5 0.0 8.611379043462779E-5 2.3373743117970398E-4 50-51 0.0010661707387144392 2.8704596811542594E-5 0.0 9.021444712199102E-5 2.542407146165201E-4 52-53 0.0011440832157743404 3.6905910186269046E-5 0.0 9.021444712199102E-5 2.542407146165201E-4 54-55 0.001226096349521605 4.100656687363228E-5 0.0 9.431510380935424E-5 2.542407146165201E-4 56-57 0.0013081094832688696 4.100656687363228E-5 0.0 9.841576049671747E-5 2.542407146165201E-4 58-59 0.0013696193335793181 4.100656687363228E-5 0.0 1.0251641718408069E-4 2.70643341365973E-4 60-61 0.0014844377208254886 4.100656687363228E-5 0.0 1.0661707387144393E-4 2.7884465474069947E-4 62-63 0.0016156587348211118 4.100656687363228E-5 0.0 1.0661707387144393E-4 2.7884465474069947E-4 64-65 0.0017673830322535512 4.100656687363228E-5 0.0 1.0661707387144393E-4 2.7884465474069947E-4 66-67 0.0019027047029365376 4.100656687363228E-5 0.0 1.0661707387144393E-4 2.7884465474069947E-4 68-69 0.002091334910555246 4.100656687363228E-5 0.0 1.1071773055880715E-4 2.952472814901524E-4 70-71 0.002308669714985497 4.100656687363228E-5 0.0 1.1481838724617038E-4 2.9934793817751565E-4 72-73 0.002484997952542116 4.100656687363228E-5 0.0 1.2301970062089684E-4 3.116499082396053E-4 74-75 0.0026531248767240083 4.920788024835873E-5 0.0 1.2712035730826006E-4 3.116499082396053E-4 76-77 0.002936070188152071 4.920788024835873E-5 0.0 1.3942232737034974E-4 3.116499082396053E-4 78-79 0.003210814186205407 4.920788024835873E-5 0.0 1.476236407450762E-4 3.116499082396053E-4 80-81 0.0034855581842587434 4.920788024835873E-5 0.0 1.476236407450762E-4 3.116499082396053E-4 82-83 0.003953033046618152 4.920788024835873E-5 0.0 1.476236407450762E-4 3.116499082396053E-4 84-85 0.0047321578172171645 5.330853693572196E-5 0.0 1.5582495411980266E-4 3.23951878301695E-4 86-87 0.005884442346366232 5.740919362308519E-5 0.0 1.5582495411980266E-4 3.444551617385111E-4 88-89 0.0073401754703801775 5.740919362308519E-5 0.0 1.640262674945291E-4 3.567571318006008E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AAGCAGT 14390 0.0 28.345234 1 AGTGGTA 17035 0.0 23.047493 5 CGCGTAG 390 0.0 21.880846 54-55 GGTATCA 18115 0.0 21.306961 8 GTATCAA 18355 0.0 21.182234 9 CGTCACG 840 0.0 20.887756 3 TGGTATC 19140 0.0 20.413656 7 AGCAGTG 20125 0.0 20.17213 2 ATCAACG 16620 0.0 19.867601 10-11 CAACGCA 16930 0.0 19.433805 12-13 GTGGTAT 20395 0.0 19.250504 6 GCAGTGG 21450 0.0 18.570402 3 ACTCGCG 505 0.0 17.367437 50-51 ACGCAGA 19065 0.0 17.26994 14-15 CTCGCGT 500 0.0 17.067026 52-53 CAGTGGT 23490 0.0 16.835348 4 AGAGTAC 19825 0.0 16.13028 18-19 TCAACGC 16560 0.0 15.945943 10-11 ACGTACG 575 0.0 15.667025 20-21 CGTACGC 635 0.0 15.306676 20-21 >>END_MODULE