FastQCFastQC Report
Sat 18 Jun 2016
SRR578604_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR578604_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9488408
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT161102516.978875697588048No Hit
AAGCAGTGGTATCAACGCAGAGTATTTTTTTTTTTTTTTTTTTTTTTTTT327270.3449156064958421No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAG219680.23152461403430374No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATCCGCAGCGGCTTCTGA106790.11254785839732019No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT3384150.092.549271
AGTGGTA3379750.092.0122765
AGCAGTG3407900.091.8213042
GGTATCA3396750.091.633518
TGGTATC3398800.091.5944067
GTATCAA3397300.091.4967969
GCAGTGG3410900.091.4816063
GTGGTAT3405500.091.479046
CAGTGGT3413700.091.102234
CAACGCA3401300.046.19507212-13
ATCAACG3398750.046.1839410-11
TCAACGC3399450.046.1650112-13
ACGCAGA3416500.046.05121214-15
AACGCAG3410800.046.043914-15
CGCAGAG3424900.045.92891716-17
AGAGTAC3339700.045.9108818-19
TATCAAC3413700.045.8452910-11
CAGAGTA3395500.045.78797518-19
GAGTACT1937100.045.72470520-21
GCAGAGT3429200.045.6413916-17
Produced by FastQC (version 0.11.5)