##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR578604_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 9488408 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.67177444308887 31.0 31.0 34.0 27.0 34.0 2 30.970508856701777 31.0 31.0 34.0 27.0 34.0 3 31.08142872861285 31.0 31.0 34.0 27.0 34.0 4 34.479747814385725 37.0 35.0 37.0 30.0 37.0 5 34.564166612565565 37.0 35.0 37.0 30.0 37.0 6 34.56630690838758 37.0 35.0 37.0 30.0 37.0 7 34.512219963559744 37.0 35.0 37.0 31.0 37.0 8 34.49489861734445 37.0 35.0 37.0 30.0 37.0 9 35.8937465589591 39.0 35.0 39.0 30.0 39.0 10-11 35.830912783261425 39.0 35.0 39.0 30.0 39.0 12-13 35.93176405356937 39.0 35.0 39.0 30.0 39.0 14-15 37.024076957904846 40.0 37.0 41.0 31.0 41.0 16-17 36.96746967457554 40.0 36.5 41.0 30.5 41.0 18-19 36.92141600571982 40.0 36.5 41.0 30.5 41.0 20-21 36.756152033091325 39.5 36.0 41.0 30.0 41.0 22-23 36.43076298995574 39.5 36.0 40.5 29.0 41.0 24-25 36.358363331340726 39.0 36.0 40.0 30.0 41.0 26-27 36.28590006879974 39.0 36.0 41.0 29.0 41.0 28-29 36.06125885396159 39.0 35.5 41.0 27.0 41.0 30-31 35.78586950518991 39.0 35.0 41.0 26.5 41.0 32-33 35.367280422595655 38.0 34.0 40.0 25.0 41.0 34-35 35.160923571161774 38.0 34.0 40.0 25.0 41.0 36-37 34.78322707033677 38.0 33.5 39.5 25.0 41.0 38-39 33.85948032588818 35.5 33.0 39.0 24.0 41.0 40-41 33.98619842232753 36.0 33.0 40.0 24.0 41.0 42-43 33.87616563284378 36.0 33.0 40.0 23.0 41.0 44-45 33.731332326771785 36.0 33.0 40.0 23.0 41.0 46-47 33.52330106378225 35.5 33.0 40.0 22.5 41.0 48-49 33.1777414082531 35.0 33.0 40.0 20.5 41.0 50-51 31.927095040601124 35.0 31.0 39.0 15.5 40.5 52-53 31.449185205779514 35.0 31.0 39.0 7.5 40.0 54-55 30.765300722734523 35.0 29.0 39.0 2.0 41.0 56-57 30.11983164088222 35.0 28.0 39.0 2.0 41.0 58-59 29.824526042724976 35.0 27.0 39.0 2.0 41.0 60-61 29.571690266691736 35.0 27.0 39.0 2.0 41.0 62-63 29.249361431338116 35.0 26.5 39.0 2.0 41.0 64-65 28.919755400484465 35.0 26.0 38.0 2.0 40.0 66-67 28.608379245496188 35.0 26.0 38.0 2.0 40.0 68-69 28.27632159156731 35.0 26.0 37.0 2.0 40.0 70-71 27.908621762470585 34.0 25.5 36.5 2.0 39.0 72-73 27.523022197190507 34.0 24.5 36.0 2.0 39.0 74-75 27.16504992196794 34.0 24.0 35.5 2.0 39.0 76-77 26.783433163919597 34.0 22.5 35.0 2.0 37.5 78-79 26.441624453754518 34.0 21.5 35.0 2.0 37.0 80-81 26.09249976392246 33.5 20.0 35.0 2.0 37.0 82-83 25.73083677472554 33.0 19.5 35.0 2.0 36.0 84-85 25.38963406716912 33.0 18.0 35.0 2.0 36.0 86-87 25.065958852106696 33.0 13.5 35.0 2.0 35.0 88-89 24.71015016428467 32.5 7.5 35.0 2.0 35.0 90-91 24.34285509223465 32.0 4.5 35.0 2.0 35.0 92-93 23.968971875998587 32.0 2.0 35.0 2.0 35.0 94-95 23.53856964203057 32.0 2.0 35.0 2.0 35.0 96-97 23.041451685045583 31.0 2.0 35.0 2.0 35.0 98-99 22.50589118849021 31.0 2.0 35.0 2.0 35.0 100-101 21.19897078624781 29.0 2.0 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.613830728362263 1101 2 0.6705447571448389 1101 3 0.6576583387505153 1101 4 0.7215885752449296 1101 5 0.6848347530533232 1101 6 0.5696736523859443 1101 7 0.5257217390250091 1101 8 0.6380641570348615 1101 9 0.7197361799966586 1101 10-11 0.7996066477542882 1101 12-13 0.7982326997800797 1101 14-15 0.87476461015914 1101 16-17 0.8564567848683637 1101 18-19 0.849425915397191 1101 20-21 0.8818086109300225 1101 22-23 0.9396142428081546 1101 24-25 0.9281468385644871 1101 26-27 1.1344707076493634 1101 28-29 1.1118432250771448 1101 30-31 1.2164645006981871 1101 32-33 1.1854351298281287 1101 34-35 1.3183917022628506 1101 36-37 1.2498973750482207 1101 38-39 1.2060543293496266 1101 40-41 1.2862237773787726 1101 42-43 1.532575648864416 1101 44-45 1.414518724055668 1101 46-47 1.4774492308674994 1101 48-49 1.4235596520288354 1101 50-51 1.350962397923464 1101 52-53 1.2897589618565526 1101 54-55 1.174350638791939 1101 56-57 1.1782374054283409 1101 58-59 1.1232131794618603 1101 60-61 1.218349445358193 1101 62-63 1.2501035465744827 1101 64-65 1.0646991060318562 1101 66-67 1.1185952246345572 1101 68-69 1.1275900223003852 1101 70-71 1.2363720021625575 1101 72-73 1.2992687453366223 1101 74-75 1.33866124527313 1101 76-77 1.370514131439176 1101 78-79 1.2875991857166476 1101 80-81 1.3209008796446469 1101 82-83 1.3066630206635672 1101 84-85 1.3417506468937432 1101 86-87 1.356901979684487 1101 88-89 1.428982395489804 1101 90-91 1.3967063565937927 1101 92-93 1.5817274376636838 1101 94-95 1.6841504641488747 1101 96-97 1.7641190665879272 1101 98-99 1.8496600665244323 1101 100-101 2.102831043140114 1102 1 0.5328032022992453 1102 2 0.5102529718237783 1102 3 0.49371176586535626 1102 4 0.6495983638256106 1102 5 0.5082253363739753 1102 6 0.4690030923631099 1102 7 0.3313767063993538 1102 8 0.4822341754915982 1102 9 0.6438272295009995 1102 10-11 0.6818955320240434 1102 12-13 0.6114001244043337 1102 14-15 0.6724483999583057 1102 16-17 0.6882759321692831 1102 18-19 0.644076090770362 1102 20-21 0.7927701806240393 1102 22-23 0.745315568025454 1102 24-25 0.7376225759893131 1102 26-27 0.8490204259885488 1102 28-29 0.7530504608178745 1102 30-31 0.9409610476619292 1102 32-33 0.8917653199075701 1102 34-35 0.86779282096186 1102 36-37 0.9258143389457842 1102 38-39 0.8920468314447305 1102 40-41 1.0606843628428138 1102 42-43 1.1563731459053912 1102 44-45 1.0034940264523513 1102 46-47 1.104009534582353 1102 48-49 1.0264250432021313 1102 50-51 1.0904727927900701 1102 52-53 1.0444375441301865 1102 54-55 0.9869356617931722 1102 56-57 1.0550418851516632 1102 58-59 0.8853518447782243 1102 60-61 1.0403357016767636 1102 62-63 1.097780507031743 1102 64-65 1.01493126907366 1102 66-67 1.14183632984901 1102 68-69 1.0905748683853709 1102 70-71 1.0352428217212442 1102 72-73 1.1665328797880505 1102 74-75 1.1541977889713522 1102 76-77 1.1458802401023327 1102 78-79 1.065857354075618 1102 80-81 1.0879438314679177 1102 82-83 1.0804950936838758 1102 84-85 1.0434926840572878 1102 86-87 1.143359102094145 1102 88-89 1.307304717174901 1102 90-91 1.3064853374394758 1102 92-93 1.401609629786595 1102 94-95 1.3779384615922758 1102 96-97 1.4733325882435615 1102 98-99 1.6185494163751422 1102 100-101 1.8598756155391563 1103 1 0.4606062236796973 1103 2 0.5574795719574297 1103 3 0.5360779284736559 1103 4 0.610205155584346 1103 5 0.5519481954242877 1103 6 0.4416103444741424 1103 7 0.40367241801928344 1103 8 0.47345530967338334 1103 9 0.5172522331685201 1103 10-11 0.6296221056990561 1103 12-13 0.6427165245259729 1103 14-15 0.7858607142402505 1103 16-17 0.6563365758804665 1103 18-19 0.6153279430094116 1103 20-21 0.7463363012514463 1103 22-23 0.6036096748252433 1103 24-25 0.6671206770683611 1103 26-27 0.8146338126081574 1103 28-29 0.9268673686462066 1103 30-31 0.9337635883968787 1103 32-33 0.9523688535633781 1103 34-35 0.9207187555666607 1103 36-37 0.9186716691792043 1103 38-39 0.9377659510364538 1103 40-41 0.903096326114742 1103 42-43 0.9992524339018658 1103 44-45 0.9331791886922147 1103 46-47 0.8598584857023894 1103 48-49 0.9984306814417963 1103 50-51 1.000074747083712 1103 52-53 0.9238243543094775 1103 54-55 0.8857637593974133 1103 56-57 0.7313379263263364 1103 58-59 0.7033346103605815 1103 60-61 0.7696841549031568 1103 62-63 0.7366613301671308 1103 64-65 0.7997723974971649 1103 66-67 0.8285242721717196 1103 68-69 0.7968727275556589 1103 70-71 0.7869214495064192 1103 72-73 0.8519664471015211 1103 74-75 0.7436341186568498 1103 76-77 0.7573356751398812 1103 78-79 0.7772186581293816 1103 80-81 0.9471155440156984 1103 82-83 0.9489497526516324 1103 84-85 0.9962033428463499 1103 86-87 1.0513227137326666 1103 88-89 1.1018876755878821 1103 90-91 1.1168246720879438 1103 92-93 1.1757857542232273 1103 94-95 1.2082209046848469 1103 96-97 1.2734150748449373 1103 98-99 1.3432881596671713 1103 100-101 1.6492560625185995 1104 1 0.31763293311293594 1104 2 0.27262208053104686 1104 3 0.35790887625998735 1104 4 0.42203762678471435 1104 5 0.3488422131404505 1104 6 0.25361087884608935 1104 7 0.3232384896717022 1104 8 0.34912137243922103 1104 9 0.43681321836677256 1104 10-11 0.48127437728006583 1104 12-13 0.47823686500397145 1104 14-15 0.5196194874278675 1104 16-17 0.5293646605900264 1104 18-19 0.4709105828824889 1104 20-21 0.5421723365008262 1104 22-23 0.4637593199883199 1104 24-25 0.6089733524213514 1104 26-27 0.5812469601085084 1104 28-29 0.48838192595192 1104 30-31 0.516999574984446 1104 32-33 0.5681764853800999 1104 34-35 0.6212673023246751 1104 36-37 0.7113789781279962 1104 38-39 0.6581581952615778 1104 40-41 0.7123518041628216 1104 42-43 0.7397486675296889 1104 44-45 0.7962051501224323 1104 46-47 0.6995150802649945 1104 48-49 0.7624571042680444 1104 50-51 0.6909589153227635 1104 52-53 0.7270380180891358 1104 54-55 0.7275536695874472 1104 56-57 0.5859429055698193 1104 58-59 0.575754348846214 1104 60-61 0.46035135014069084 1104 62-63 0.5785871348588927 1104 64-65 0.6234287307589526 1104 66-67 0.4517241087830435 1104 68-69 0.49146913507295054 1104 70-71 0.5727595756667903 1104 72-73 0.5792285046672383 1104 74-75 0.5718976023634283 1104 76-77 0.6194093241377168 1104 78-79 0.5253045854912841 1104 80-81 0.45608565306843474 1104 82-83 0.4561350942978315 1104 84-85 0.5697498654478181 1104 86-87 0.577253341475938 1104 88-89 0.6658093763098307 1104 90-91 0.6346478597107605 1104 92-93 0.6877974472667319 1104 94-95 0.7642050990960207 1104 96-97 0.8236646705138675 1104 98-99 0.985528685557771 1104 100-101 1.2194567491983186 1105 1 0.1407813024569542 1105 2 0.17757753168215018 1105 3 0.2989469042545352 1105 4 0.3541769447936005 1105 5 0.4456143017603651 1105 6 0.3514447076581817 1105 7 0.39816306187888273 1105 8 0.4218549995206686 1105 9 0.4090509243001321 1105 10-11 0.3617865784444376 1105 12-13 0.3617053490159279 1105 14-15 0.20638069321081076 1105 16-17 0.2777681793739788 1105 18-19 0.26648246269349585 1105 20-21 0.5888020855762335 1105 22-23 0.5928448077694597 1105 24-25 0.6073381619274656 1105 26-27 0.7168743158445636 1105 28-29 0.672879191970928 1105 30-31 0.7213596774423721 1105 32-33 0.7513852854367329 1105 34-35 0.7111906137153738 1105 36-37 0.7777924861175833 1105 38-39 0.8430093746779761 1105 40-41 0.7305296810363515 1105 42-43 0.9062969944041015 1105 44-45 0.87100253470242 1105 46-47 0.898779382790174 1105 48-49 1.0042579262319151 1105 50-51 0.9532817374281883 1105 52-53 0.8124815600544117 1105 54-55 0.8682215262879147 1105 56-57 0.8428934329007838 1105 58-59 0.9814845385437927 1105 60-61 0.8726140069685613 1105 62-63 0.8192698116828723 1105 64-65 0.9294338669271305 1105 66-67 1.0515249218990732 1105 68-69 0.9683967465114307 1105 70-71 0.7916986981385215 1105 72-73 0.8688656991957338 1105 74-75 0.8047079180541772 1105 76-77 0.7822222057006023 1105 78-79 0.7925998908300649 1105 80-81 0.7560173035435618 1105 82-83 0.8097471123120208 1105 84-85 0.7709783873285119 1105 86-87 0.8537017320547591 1105 88-89 0.9575540145218611 1105 90-91 1.001023432608072 1105 92-93 1.073742219081069 1105 94-95 1.1478627055797155 1105 96-97 1.2378633442234097 1105 98-99 1.3983858062953267 1105 100-101 1.5914800057324463 1106 1 0.11313339639174558 1106 2 0.23322020198088467 1106 3 0.3278975036370184 1106 4 0.3920240301728626 1106 5 0.2806479062596381 1106 6 0.26195762398093336 1106 7 0.2647836357063298 1106 8 0.2489621745119308 1106 9 0.15033984243726195 1106 10-11 0.26854577367152643 1106 12-13 0.33890819014425233 1106 14-15 0.3315494179955536 1106 16-17 0.34496724776777654 1106 18-19 0.24853673264819776 1106 20-21 0.3236780521522604 1106 22-23 0.40461383916505866 1106 24-25 0.4225107048889214 1106 26-27 0.351971023020873 1106 28-29 0.4094837432953966 1106 30-31 0.5456806420313356 1106 32-33 0.5093646550957232 1106 34-35 0.5527305638033297 1106 36-37 0.5188040364894277 1106 38-39 0.7387569358782784 1106 40-41 0.5274216624728041 1106 42-43 0.435404173113362 1106 44-45 0.568250638317231 1106 46-47 0.6074470880174232 1106 48-49 0.5637533661081804 1106 50-51 0.6358613500789652 1106 52-53 0.7126134663377464 1106 54-55 0.41816605066929924 1106 56-57 0.3285972425674082 1106 58-59 0.6262514521267981 1106 60-61 0.49578588833305304 1106 62-63 0.577587937374318 1106 64-65 0.6838877850353491 1106 66-67 0.681247764820295 1106 68-69 0.7069025215940741 1106 70-71 0.6422681868891011 1106 72-73 0.6813717512691788 1106 74-75 0.6732569189869793 1106 76-77 0.5976742181286276 1106 78-79 0.6437996059488498 1106 80-81 0.6438286238539881 1106 82-83 0.5969047312321685 1106 84-85 0.6310156168751888 1106 86-87 0.7328828472721938 1106 88-89 0.6765434638781933 1106 90-91 0.7096126674359589 1106 92-93 0.7477441226957033 1106 94-95 0.7454534846158261 1106 96-97 0.8908969873485404 1106 98-99 0.9796322019034598 1106 100-101 1.2219260920858268 1107 1 0.07205712979222056 1107 2 0.08815299093582851 1107 3 0.2439945812850084 1107 4 0.46042041225493335 1107 5 0.17534360078052913 1107 6 0.2329032951762926 1107 7 0.26473881950654743 1107 8 0.20974780218224964 1107 9 0.17719714827050836 1107 10-11 0.2406476789109817 1107 12-13 0.23238964217872393 1107 14-15 0.3602918006840099 1107 16-17 0.2889085232642685 1107 18-19 0.42105775408122526 1107 20-21 0.31953922776195043 1107 22-23 0.2811860849634584 1107 24-25 0.5370251916397351 1107 26-27 0.20811505528022423 1107 28-29 0.2482927270566222 1107 30-31 0.3969279145040119 1107 32-33 0.5681901743817335 1107 34-35 0.34973964643879185 1107 36-37 0.39495266992020106 1107 38-39 0.5538607177116432 1107 40-41 0.41557238815789077 1107 42-43 0.47123056133049346 1107 44-45 0.7077019148382178 1107 46-47 0.5356008409531299 1107 48-49 0.6269595727620114 1107 50-51 0.43829998652919144 1107 52-53 0.6066578757507308 1107 54-55 0.5897229312476426 1107 56-57 0.6067179395274067 1107 58-59 0.7980538757881277 1107 60-61 0.8218657147069237 1107 62-63 0.6786114225288102 1107 64-65 0.8149292564454775 1107 66-67 0.9355419307251687 1107 68-69 0.8550959094737074 1107 70-71 0.7868770636673723 1107 72-73 0.7172955999068584 1107 74-75 0.8462602525491931 1107 76-77 0.8271662195908966 1107 78-79 0.7401397410492656 1107 80-81 0.6963209830869665 1107 82-83 0.6877263506459492 1107 84-85 0.7380468066527719 1107 86-87 0.8970233017205196 1107 88-89 0.8706610491738651 1107 90-91 0.887279240419339 1107 92-93 0.9207662934080041 1107 94-95 0.8491381850295134 1107 96-97 0.9048185508902158 1107 98-99 0.9115201599485943 1107 100-101 1.0468356709065922 1108 1 0.12026350366616256 1108 2 0.07608138220551908 1108 3 0.1279400359044338 1108 4 0.29751295664775057 1108 5 0.1042504656235721 1108 6 0.20456734998104764 1108 7 0.20416523383592988 1108 8 0.17790825697721857 1108 9 0.2490412600692764 1108 10-11 0.2439391271907212 1108 12-13 0.13122417131304331 1108 14-15 0.3133945756129677 1108 16-17 0.18432325531063753 1108 18-19 0.17773513422860532 1108 20-21 0.20004522161714533 1108 22-23 0.15080232986226605 1108 24-25 0.3561018858537963 1108 26-27 0.37745695799178947 1108 28-29 0.2923030984278796 1108 30-31 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32-33 -0.12795598181978107 2311 34-35 -0.02151300560480962 2311 36-37 0.12438532187823625 2311 38-39 0.09790658275309028 2311 40-41 -0.09215151893794626 2311 42-43 -0.03351715750870454 2311 44-45 -0.05316537510047681 2311 46-47 0.15778517951726911 2311 48-49 -0.0697673855503993 2311 50-51 0.02535774686417369 2311 52-53 8.47739012375115E-4 2311 54-55 0.10215833831261278 2311 56-57 0.1697730837432765 2311 58-59 0.017519170260396066 2311 60-61 0.02580644944894317 2311 62-63 -0.08901958684507605 2311 64-65 -0.023596854759468044 2311 66-67 0.03264482932987889 2311 68-69 -0.014420268046208662 2311 70-71 0.08974449031734011 2311 72-73 0.013647981899577388 2311 74-75 0.019499769163576985 2311 76-77 0.07477542581338881 2311 78-79 0.06599518444418351 2311 80-81 0.0747716669985401 2311 82-83 0.170791835736825 2311 84-85 0.12188626672215008 2311 86-87 0.20459455967341 2311 88-89 0.3138806303961985 2311 90-91 0.26752470132452544 2311 92-93 0.12714108144763614 2311 94-95 0.10831241206230047 2311 96-97 0.2833424968131588 2311 98-99 0.31943922699446503 2311 100-101 0.3287165879719147 2312 1 0.14102879387534273 2312 2 -0.09678944902283249 2312 3 0.10017297172692707 2312 4 0.2900648480147865 2312 5 0.24591021697002446 2312 6 0.13833525556334791 2312 7 0.14034684592596847 2312 8 0.10928515751403722 2312 9 0.1334070884481875 2312 10-11 0.08914487438543262 2312 12-13 0.07809988257161393 2312 14-15 0.08261456866137706 2312 16-17 -0.00579090481465272 2312 18-19 0.07501686937312257 2312 20-21 0.047296413688961536 2312 22-23 0.10784615446661405 2312 24-25 0.11174196906896583 2312 26-27 0.04093943581522552 2312 28-29 0.040619921250410584 2312 30-31 0.028768911486871218 2312 32-33 -0.025309045827846433 2312 34-35 0.039279170514475936 2312 36-37 0.06606715932862528 2312 38-39 0.045991019482301 2312 40-41 0.03143125180098849 2312 42-43 0.022225054988275872 2312 44-45 0.14949019379893258 2312 46-47 0.1691216730922278 2312 48-49 0.19957861923654008 2312 50-51 -0.018032896871897464 2312 52-53 0.12163523252620578 2312 54-55 0.06241615124708133 2312 56-57 0.029497127148953695 2312 58-59 -0.030342220328645197 2312 60-61 -0.06205757084474328 2312 62-63 -0.0627768886225688 2312 64-65 0.027301680005567874 2312 66-67 0.018300721178160728 2312 68-69 -0.01793767993884643 2312 70-71 -0.024461560255410575 2312 72-73 0.025227625488422944 2312 74-75 0.035078911630144205 2312 76-77 0.04357797873342051 2312 78-79 0.05361845232828344 2312 80-81 -0.05523413627626539 2312 82-83 -0.03867800416295708 2312 84-85 -0.005963435354452429 2312 86-87 0.039914131983699974 2312 88-89 -0.05177669848264799 2312 90-91 0.024886476372863342 2312 92-93 -0.0683980838398277 2312 94-95 -0.20862092488658845 2312 96-97 -0.03842429166406447 2312 98-99 -0.07107514951581706 2312 100-101 -0.17332417160751135 2313 1 -0.6605857031078415 2313 2 -0.4681518617296305 2313 3 -0.24887426149681957 2313 4 -0.029352935528009994 2313 5 -0.33179547118977837 2313 6 -0.3236135429026703 2313 7 -0.36148368360843364 2313 8 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-0.6063586101562812 2313 72-73 -0.6256811419147468 2313 74-75 -0.7528822066987644 2313 76-77 -0.7986558403806576 2313 78-79 -0.629346665664194 2313 80-81 -0.8423088744826366 2313 82-83 -0.7405421424751211 2313 84-85 -0.7067370030247666 2313 86-87 -0.8051116613219662 2313 88-89 -0.7511036420753463 2313 90-91 -0.6734337600266755 2313 92-93 -0.6133084471124128 2313 94-95 -0.6092372868937375 2313 96-97 -0.6483782110980556 2313 98-99 -0.607493816292834 2313 100-101 -0.7356461592983301 2314 1 -0.529691292617386 2314 2 -0.7843297738131305 2314 3 -0.5205838234310747 2314 4 -0.7149824051957623 2314 5 -0.6433004013285597 2314 6 -0.439171786394823 2314 7 -0.38370520796932794 2314 8 -0.41758669188092057 2314 9 -0.3859448718246625 2314 10-11 -0.4821962182224695 2314 12-13 -0.47498238538785387 2314 14-15 -0.6442180464287119 2314 16-17 -0.6407872675460808 2314 18-19 -2.4042429664579927 2314 20-21 -1.059409831371994 2314 22-23 -0.7900244243462637 2314 24-25 -0.7975737106229346 2314 26-27 -0.9104492865320637 2314 28-29 -0.6339849435948537 2314 30-31 -0.6132580701061912 2314 32-33 -0.6334635366525347 2314 34-35 -0.7013580923780793 2314 36-37 -0.6929812775727271 2314 38-39 -0.5690644049832869 2314 40-41 -0.5082648801588121 2314 42-43 -0.8513005015300266 2314 44-45 -0.7991869416438178 2314 46-47 -0.6676650234372303 2314 48-49 -0.6959711371956487 2314 50-51 -0.745833089701005 2314 52-53 -0.6731326704642093 2314 54-55 -0.7954016214238031 2314 56-57 -0.7919171279084374 2314 58-59 -0.8023372682213754 2314 60-61 -0.7043928716281442 2314 62-63 -0.8675896251912931 2314 64-65 -0.8767245949876958 2314 66-67 -0.9348559054339454 2314 68-69 -0.9544548745047372 2314 70-71 -1.082359632045442 2314 72-73 -0.9908374262981958 2314 74-75 -0.9820319490023977 2314 76-77 -1.0053842093138847 2314 78-79 -0.9709042915775399 2314 80-81 -0.8738766901520414 2314 82-83 -0.9662857299322525 2314 84-85 -0.9861388300174951 2314 86-87 -1.0592240282639587 2314 88-89 -1.008123523597284 2314 90-91 -0.9801714210182766 2314 92-93 -0.8666190169431687 2314 94-95 -0.9108621519674251 2314 96-97 -0.9696569184786874 2314 98-99 -1.0586791250578962 2314 100-101 -1.1132472867360121 2315 1 -0.892207356415792 2315 2 -1.7947959883849265 2315 3 -1.390755386933936 2315 4 -0.642794806831418 2315 5 -0.9359904009312245 2315 6 -1.3826129377252414 2315 7 -1.3735528522664353 2315 8 -1.513670048021467 2315 9 -1.6034761030789326 2315 10-11 -0.8816212013884339 2315 12-13 -1.7473449395173262 2315 14-15 -8.69940199604838 2315 16-17 -4.328918419532478 2315 18-19 -1.873768709819572 2315 20-21 -1.0913784213758717 2315 22-23 -2.145535469504189 2315 24-25 -2.00393893057781 2315 26-27 -1.602513022558 2315 28-29 -1.3367761311564053 2315 30-31 -1.550943186712061 2315 32-33 -1.9291037569570548 2315 34-35 -1.8602235067575137 2315 36-37 -1.5582356554021572 2315 38-39 -1.7671540294065409 2315 40-41 -2.694142565258449 2315 42-43 -2.2799873162566264 2315 44-45 -1.958503306877816 2315 46-47 -1.674835310262747 2315 48-49 -2.0371566477123935 2315 50-51 -1.7959032200564522 2315 52-53 -1.643088371509993 2315 54-55 -1.7360753023548874 2315 56-57 -1.7832853214325262 2315 58-59 -1.9067845960204721 2315 60-61 -1.5203534714119442 2315 62-63 -1.4434896499500667 2315 64-65 -1.4640822093583417 2315 66-67 -1.4351495611193883 2315 68-69 -1.6731180253200826 2315 70-71 -1.3954779321153516 2315 72-73 -1.3479129966204617 2315 74-75 -1.4553875195891628 2315 76-77 -1.4712000864237602 2315 78-79 -1.554174528364701 2315 80-81 -1.5539572465790208 2315 82-83 -1.4911655773138044 2315 84-85 -1.5834572867856984 2315 86-87 -1.7121912834240511 2315 88-89 -1.7256230822286796 2315 90-91 -1.6681973877120768 2315 92-93 -1.7727097047473102 2315 94-95 -1.5982975010488794 2315 96-97 -1.6239319238023207 2315 98-99 -1.7476776095119178 2315 100-101 -1.9486017722903988 2316 1 -0.8819840217864261 2316 2 -0.9982082449518614 2316 3 -1.3306563885954041 2316 4 -1.2886041438936218 2316 5 -0.7665312804329574 2316 6 -0.8544929193227233 2316 7 -1.065888174444801 2316 8 -1.1384524635702107 2316 9 -1.3670732799528835 2316 10-11 -1.3921300769350395 2316 12-13 -1.151872201513065 2316 14-15 -1.2626858184450995 2316 16-17 -2.022108508061116 2316 18-19 -1.323791552687176 2316 20-21 -1.7567472808360947 2316 22-23 -1.2982864697900993 2316 24-25 -1.2443777733292123 2316 26-27 -1.2825998891609274 2316 28-29 -1.6814257427891093 2316 30-31 -1.4317618893702715 2316 32-33 -1.5844754381474857 2316 34-35 -2.129163822903287 2316 36-37 -1.8760006685771344 2316 38-39 -1.7558062227154352 2316 40-41 -2.156557019642385 2316 42-43 -2.422456561061157 2316 44-45 -2.620016623423602 2316 46-47 -2.32371937561879 2316 48-49 -2.905843827797611 2316 50-51 -2.4592067872606584 2316 52-53 -3.288023353636195 2316 54-55 -4.606629200607205 2316 56-57 -3.2122788284503727 2316 58-59 -2.6592380486309466 2316 60-61 -2.3367257675886037 2316 62-63 -2.9509375528332065 2316 64-65 -2.997679565582988 2316 66-67 -2.7539737274003677 2316 68-69 -2.779020838180127 2316 70-71 -3.0328038189051334 2316 72-73 -5.98554176088523 2316 74-75 -3.748723294039671 2316 76-77 -3.165513865905343 2316 78-79 -3.109137584149991 2316 80-81 -2.980593265782364 2316 82-83 -3.019315430014945 2316 84-85 -3.087728552036694 2316 86-87 -2.935050958059165 2316 88-89 -2.74677471739982 2316 90-91 -2.7527051029164795 2316 92-93 -3.0710837324291553 2316 94-95 -3.401200833782486 2316 96-97 -3.4147851011541093 2316 98-99 -3.8355501689932545 2316 100-101 -3.916824074963447 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 90009.0 3 16288.0 4 14548.0 5 14907.0 6 16753.0 7 21434.0 8 27435.0 9 32491.0 10 37781.0 11 46006.0 12 46557.0 13 52349.0 14 59190.0 15 69268.0 16 86582.0 17 113982.0 18 160666.0 19 240586.0 20 344656.0 21 257583.0 22 144177.0 23 131285.0 24 139238.0 25 144989.0 26 156874.0 27 174784.0 28 199175.0 29 232017.0 30 273121.0 31 323809.0 32 391839.0 33 483886.0 34 626459.0 35 895931.0 36 1312030.0 37 1192866.0 38 806078.0 39 110703.0 40 76.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 10.350632953057916 53.64993505468657 19.95949041349782 16.03994157875769 2 15.41420815196519 54.717579381734296 15.78911505284486 14.07909741345566 3 51.18407251916087 17.722442730472906 16.163517905894672 14.92996684447155 4 15.060448787460334 16.61502418659002 17.215504634306722 51.109022391642924 5 15.177723037784835 52.542008198342295 18.02879070495935 14.251478058913522 6 51.00713500256977 17.359504714612555 16.40101170277138 15.232348580046299 7 15.802008909826931 16.962324606507963 53.04471835131489 14.190948132350217 8 50.65226080052304 17.603821170551516 17.112177001183646 14.631741027741798 9 50.22573743197367 17.367178917601592 17.672841840348372 14.734241810076359 10-11 14.745971104791304 35.19646081842651 35.01463830255811 15.042929774224078 12-13 14.598236829482419 17.774281984940025 34.85789146844674 32.76958971713081 14-15 14.804235490772427 53.058206724824096 17.009894174960856 15.127663609442616 16-17 32.64194964031736 17.540520391051054 17.070300029603988 32.7472299390276 18-19 15.022506977242037 35.22548368778022 17.081796529754715 32.67021280522303 20-21 32.78192888128471 35.26665761546543 17.158957987047994 14.79245551620187 22-23 32.66613402065932 17.510411507262553 34.923923968516775 14.899530503561357 24-25 14.854622157002733 34.963437405794025 17.755826127152904 32.42611431005034 26-27 15.124247146749445 24.52215434124286 45.260272123763166 15.093326388244524 28-29 29.610051297288777 17.312187820588285 38.14497308343463 14.93278779868831 30-31 26.173038912445218 19.288050882727607 38.741753710286744 15.79715649454043 32-33 19.06938743750851 21.95770898514712 40.776605929733506 18.196297647610866 34-35 18.418800897511314 20.876649452784775 41.614613509625755 19.089936140078155 36-37 18.691802528316515 20.646642352625715 41.70740051438131 18.95415460467646 38-39 18.859480083257033 20.681563358638243 41.540146506480845 18.918810051623876 40-41 18.788670009486893 20.728875473461635 41.23258350275723 19.249871014294243 42-43 19.1768849102737 20.853225340207292 41.25926242644902 18.710627323069986 44-45 18.568191721822096 20.97110459272241 41.144385824639876 19.31631786081562 46-47 19.12175216419583 20.786555090746685 40.920035822190385 19.171656922867097 48-49 18.91787468035261 21.055974160719185 41.02991156758342 18.996239591344782 50-51 19.132587362964255 20.738275007005168 40.75343200583505 19.375705624195525 52-53 19.534957697259905 21.732081169977263 39.40854185970108 19.324419273061753 54-55 20.07264990699184 22.55053555872483 37.76009018870924 19.61672434557408 56-57 20.413703939026313 23.411171733432642 36.48723110867713 19.68789321886392 58-59 20.57682172738195 24.326845189050434 35.38365469316672 19.7126783904009 60-61 20.446811697530894 24.615429307642266 34.92923275029012 20.00852624453672 62-63 20.34029474242319 24.701556543142285 34.806103428693056 20.15204528574147 64-65 20.112422924546088 24.29031427425386 35.088585894501264 20.50867690669879 66-67 20.172456132600132 23.710406466912545 35.95658541096128 20.16055198952604 68-69 19.99493571799193 23.667225533681798 36.24731114883285 20.090527599493424 70-71 19.9796683529916 23.57989677689985 36.62209314311224 19.818341726996312 72-73 19.632505091121438 23.335895610092926 36.72043792738257 20.31116137140306 74-75 20.159608448530445 23.49867143217234 36.1157504999231 20.22596961937411 76-77 20.569127992615215 24.02762725101039 35.29106322032847 20.112181536045927 78-79 20.29479690588935 24.117427616944116 35.07651819868783 20.51125727847871 80-81 20.17411189521401 24.836430052416613 34.580108257936296 20.409349794433076 82-83 21.200388819099583 24.816171314901506 33.72989345749339 20.253546408505517 84-85 20.887083207222005 25.41260876393641 33.37018833111232 20.33011969772927 86-87 20.660758946555994 25.593126519369612 33.28156842044716 20.464546113627243 88-89 20.46391313433824 25.217038683274406 33.247559586658056 21.071488595729303 90-91 20.47131413951908 25.38958010618523 33.16923578496947 20.96986996932621 92-93 20.79684198994826 25.50788263626929 33.24701900303522 20.448256370747234 94-95 20.611554167868903 25.35237716758016 32.6329433434526 21.403125321098333 96-97 20.57485533075464 25.067977940905156 33.082743744880304 21.274422983459896 98-99 20.91639358374672 25.507518300548888 32.456025623852334 21.120062491852057 100-101 21.457131836284745 25.00144688295028 32.305997242857806 21.235424037907176 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 25462.0 1 15186.5 2 3413.5 3 1634.5 4 1226.5 5 1067.0 6 1202.5 7 1471.0 8 1353.5 9 1427.0 10 2429.0 11 17370.5 12 343956.0 13 375440.0 14 79804.5 15 65543.0 16 68102.0 17 71619.0 18 74039.5 19 74635.5 20 73804.0 21 73274.0 22 73016.0 23 73513.0 24 75333.5 25 78054.5 26 82295.0 27 88074.0 28 92826.5 29 97063.0 30 102960.5 31 109816.5 32 116824.5 33 125576.0 34 141849.0 35 152773.0 36 158703.0 37 168979.5 38 178019.0 39 187480.5 40 199398.0 41 212326.5 42 223726.0 43 234013.0 44 245850.5 45 256311.0 46 261532.5 47 277688.5 48 289365.0 49 289441.5 50 291206.5 51 289545.0 52 291123.5 53 295806.0 54 285322.5 55 265877.5 56 254832.0 57 244095.5 58 247118.0 59 219270.0 60 165940.0 61 135825.0 62 109519.0 63 85944.5 64 68161.0 65 55777.5 66 44573.5 67 35506.5 68 27920.5 69 21702.0 70 17528.5 71 14719.5 72 12525.0 73 10214.5 74 8092.5 75 6378.5 76 5074.0 77 4036.0 78 3251.0 79 2690.0 80 2290.0 81 2063.5 82 1887.0 83 1642.5 84 1444.5 85 1286.5 86 1079.5 87 881.5 88 710.0 89 555.5 90 402.0 91 304.5 92 234.5 93 169.5 94 120.5 95 85.0 96 59.5 97 35.5 98 21.5 99 11.0 100 6.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.10185059495755241 2 0.0834808115333995 3 0.09001510053109014 4 0.19487989976822245 5 0.07612446682309613 6 0.09426238837958907 7 0.09944766287453069 8 0.08832883240265385 9 0.08118327120840503 10-11 0.07861171231253969 12-13 0.07221443260028447 14-15 0.07879087830118603 16-17 0.06931615925453459 18-19 0.06105344542519672 20-21 0.05607368485840828 22-23 0.04542911729765415 24-25 0.04779516226536633 26-27 0.047299821002638166 28-29 0.04614578125223957 30-31 0.03647081786533631 32-33 0.040222764451107076 34-35 0.05347050843513474 36-37 0.04834846899500949 38-39 0.05274857489264796 40-41 0.05057750467728622 42-43 0.048422243225628575 44-45 0.052490365085481154 46-47 0.04576637092334141 48-49 0.04145584802002612 50-51 0.04574529257173596 52-53 0.0412714124434784 54-55 0.04525522089690916 56-57 0.04411698991021465 58-59 0.0511571593464362 60-61 0.050045276299248515 62-63 0.055293785849006495 64-65 0.05105176758840893 66-67 0.04547654358876642 68-69 0.045729483808031865 70-71 0.054408495081577433 72-73 0.04220412950201973 74-75 0.0495288566849149 76-77 0.04830631229179858 78-79 0.04695202820114818 80-81 0.04628806012557639 82-83 0.04538169100654187 84-85 0.046303868889280474 86-87 0.05088841036346666 88-89 0.043779736284527394 90-91 0.046530461169039104 92-93 0.04301037645092833 94-95 0.045934997736185035 96-97 0.0313856655405206 98-99 0.038483800443657144 100-101 0.026337400331014435 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 9488408.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 43.25732106559936 #Duplication Level Percentage of deduplicated Percentage of total 1 89.54546321214919 38.734968521357544 2 5.654124825678328 4.89164585858687 3 1.7684368716432748 2.294935246227518 4 0.8501684185139647 1.4710403295796572 5 0.5155902300208656 1.1151526059149406 6 0.3376650214331233 0.8763890546853065 7 0.21628680135279196 0.6549191325858463 8 0.16712137634127114 0.5783378426655381 9 0.13840177990648958 0.5388201206518896 >10 0.7798013260141057 5.5941750846792955 >50 0.009491367432830043 0.2832799871023557 >100 0.009235942287106825 0.9519550554299032 >500 0.0038824274164428138 1.1897713985917395 >1k 0.0036335538637025937 3.201432004398356 >5k 4.977471046167936E-4 1.528285024159282 >10k+ 1.9909884184671748E-4 36.094892733384 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 1611025 16.978875697588048 No Hit AAGCAGTGGTATCAACGCAGAGTATTTTTTTTTTTTTTTTTTTTTTTTTT 32727 0.3449156064958421 No Hit AAGCAGTGGTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAG 21968 0.23152461403430374 No Hit AAGCAGTGGTATCAACGCAGAGTACATGGGGAATCCGCAGCGGCTTCTGA 10679 0.11254785839732019 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 1.0539175802726863E-5 0.0 0.0 0.0 0.0 2 6.323505481636118E-5 0.0 0.0 0.0 1.0539175802726863E-5 3 7.377423061908805E-5 0.0 0.0 0.0 1.0539175802726863E-5 4 8.431340642181491E-5 0.0 0.0 0.0 1.0539175802726863E-5 5 9.485258222454178E-5 0.0 0.0 0.0 1.0539175802726863E-5 6 9.485258222454178E-5 0.0 0.0 1.0539175802726863E-5 1.0539175802726863E-5 7 1.0539175802726865E-4 0.0 0.0 1.0539175802726863E-5 1.0539175802726863E-5 8 1.0539175802726865E-4 0.0 0.0 1.0539175802726863E-5 2.1078351605453727E-5 9 1.475484612381761E-4 0.0 0.0 1.0539175802726863E-5 2.1078351605453727E-5 10-11 1.5808763704090297E-4 0.0 0.0 1.0539175802726863E-5 4.2156703210907454E-5 12-13 2.0024434025181043E-4 0.0 0.0 1.5808763704090295E-5 4.2156703210907454E-5 14-15 2.107835160545373E-4 0.0 0.0 2.1078351605453727E-5 4.2156703210907454E-5 16-17 2.2659227975862757E-4 0.0 0.0 2.1078351605453727E-5 4.2156703210907454E-5 18-19 2.5820980716680813E-4 0.0 0.0 2.1078351605453727E-5 5.2695879013634324E-5 20-21 3.003665103777156E-4 1.0539175802726863E-5 0.0 2.1078351605453727E-5 5.2695879013634324E-5 22-23 3.3725362568725963E-4 1.0539175802726863E-5 0.0 2.1078351605453727E-5 5.2695879013634324E-5 24-25 3.89949504700894E-4 2.1078351605453727E-5 0.0 2.1078351605453727E-5 5.2695879013634324E-5 26-27 4.215670321090746E-4 2.1078351605453727E-5 0.0 2.1078351605453727E-5 5.2695879013634324E-5 28-29 4.3737579581316487E-4 2.1078351605453727E-5 0.0 3.161752740818059E-5 5.2695879013634324E-5 30-31 4.900716748267992E-4 2.1078351605453727E-5 0.0 4.2156703210907454E-5 5.2695879013634324E-5 32-33 5.691154933472507E-4 2.1078351605453727E-5 0.0 4.742629111227089E-5 5.796546691499775E-5 34-35 6.481593118677022E-4 3.161752740818059E-5 0.0 5.2695879013634324E-5 6.323505481636118E-5 36-37 7.430118940922438E-4 3.161752740818059E-5 0.0 6.850464271772462E-5 6.323505481636118E-5 38-39 8.378644763167857E-4 3.161752740818059E-5 0.0 9.485258222454178E-5 6.323505481636118E-5 40-41 9.327170585413275E-4 3.161752740818059E-5 0.0 9.485258222454178E-5 7.377423061908804E-5 42-43 9.906825254563253E-4 3.161752740818059E-5 0.0 1.0012217012590521E-4 8.431340642181491E-5 44-45 0.0011066134592863208 3.161752740818059E-5 0.0 1.0539175802726865E-4 8.431340642181491E-5 46-47 0.0011382309866945013 4.742629111227089E-5 0.0 1.0539175802726865E-4 1.0012217012590521E-4 48-49 0.0011751181020040454 5.2695879013634324E-5 0.0 1.0539175802726865E-4 1.0539175802726865E-4 50-51 0.00124362274472177 5.2695879013634324E-5 0.0 1.0539175802726865E-4 1.0539175802726865E-4 52-53 0.0012963186237354043 5.2695879013634324E-5 0.0 1.0539175802726865E-4 1.0539175802726865E-4 54-55 0.0013332057390449481 5.2695879013634324E-5 0.0 1.0539175802726865E-4 1.1066134592863208E-4 56-57 0.001401710381762673 5.2695879013634324E-5 0.0 1.0539175802726865E-4 1.159309338299955E-4 58-59 0.0014912933760858511 5.2695879013634324E-5 0.0 1.159309338299955E-4 1.159309338299955E-4 60-61 0.0015334500792967588 5.796546691499775E-5 0.0 1.159309338299955E-4 1.2647010963272236E-4 62-63 0.0016019547220144834 6.323505481636118E-5 0.0 1.159309338299955E-4 1.2647010963272236E-4 64-65 0.001707346480041752 6.323505481636118E-5 0.0 1.2647010963272236E-4 1.475484612381761E-4 66-67 0.001818007825970384 6.323505481636118E-5 0.0 1.317396975340858E-4 1.475484612381761E-4 68-69 0.001902321232392199 6.323505481636118E-5 0.0 1.3700928543544923E-4 1.475484612381761E-4 70-71 0.0020709480452358285 7.377423061908805E-5 0.0 1.3700928543544923E-4 1.475484612381761E-4 72-73 0.002234305270178095 7.904381852045147E-5 0.0 1.3700928543544923E-4 1.475484612381761E-4 74-75 0.0023976624951203614 8.431340642181491E-5 0.0 1.475484612381761E-4 1.5281804913953954E-4 76-77 0.002587367659569445 8.431340642181491E-5 0.0 1.475484612381761E-4 1.5808763704090297E-4 78-79 0.002745455296610348 8.431340642181491E-5 0.0 1.5281804913953954E-4 1.5808763704090297E-4 80-81 0.002961508400566249 8.431340642181491E-5 0.0 1.6862681284362982E-4 1.5808763704090297E-4 82-83 0.0033251099657603256 8.431340642181491E-5 0.0 1.6862681284362982E-4 1.633572249422664E-4 84-85 0.003920573398614393 8.431340642181491E-5 0.0 1.6862681284362982E-4 1.7389640074499325E-4 86-87 0.004911255924070719 8.431340642181491E-5 0.0 1.7916598864635669E-4 1.7916598864635669E-4 88-89 0.0061917657841020326 8.431340642181491E-5 0.0 1.94974752350447E-4 1.8970516444908356E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AAGCAGT 338415 0.0 92.54927 1 AGTGGTA 337975 0.0 92.012276 5 AGCAGTG 340790 0.0 91.821304 2 GGTATCA 339675 0.0 91.63351 8 TGGTATC 339880 0.0 91.594406 7 GTATCAA 339730 0.0 91.496796 9 GCAGTGG 341090 0.0 91.481606 3 GTGGTAT 340550 0.0 91.47904 6 CAGTGGT 341370 0.0 91.10223 4 CAACGCA 340130 0.0 46.195072 12-13 ATCAACG 339875 0.0 46.18394 10-11 TCAACGC 339945 0.0 46.16501 12-13 ACGCAGA 341650 0.0 46.051212 14-15 AACGCAG 341080 0.0 46.0439 14-15 CGCAGAG 342490 0.0 45.928917 16-17 AGAGTAC 333970 0.0 45.91088 18-19 TATCAAC 341370 0.0 45.84529 10-11 CAGAGTA 339550 0.0 45.787975 18-19 GAGTACT 193710 0.0 45.724705 20-21 GCAGAGT 342920 0.0 45.64139 16-17 >>END_MODULE