FastQCFastQC Report
Sat 18 Jun 2016
SRR578604_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR578604_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9488408
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2962423.122146518151412No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT215230.2268346808020903No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT203850.21484109873858714No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT107550.023.7916831
AGTGGTA117200.021.236245
GGTATCA123000.020.3889988
GTATCAA126950.019.7920179
ATCAACG109100.019.5691510-11
CAACGCA111100.019.13136912-13
TGGTATC131950.018.8260577
GTGGTAT138400.018.1890136
AGCAGTG143100.018.1233442
ACGCAGA126400.016.92844614-15
TCAACGC107800.016.72091710-11
GCAGTGG154000.016.686373
AGAGTAC132950.015.84435818-19
AACGCAG116550.015.64897912-13
CAGTGGT167050.015.29752354
CGCAGAG128100.014.21954214-15
AGTACTT109050.014.19907820-21
GCAGAGT158700.013.63266616-17
TATCAAC144800.012.7599249
CAGAGTA144250.012.512316-17
Produced by FastQC (version 0.11.5)