FastQCFastQC Report
Sat 18 Jun 2016
SRR578603_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR578603_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9419489
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT161387517.133360419020608No Hit
AAGCAGTGGTATCAACGCAGAGTATTTTTTTTTTTTTTTTTTTTTTTTTT331770.3522165586689469No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA218500.2319658741572924No Hit
AAGCAGTGGTATCAACGCAGAGTACCTTTTTTTTTTTTTTTTTTTTTTTT94460.10028144838854847No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT3388100.092.373161
AGTGGTA3376400.091.934455
GGTATCA3398700.091.597278
TGGTATC3393450.091.595967
AGCAGTG3411500.091.5677262
GTATCAA3402850.091.50549
GTGGTAT3403300.091.447726
GCAGTGG3416100.091.328343
CAGTGGT3426200.090.826084
TCAACGC3405500.046.1795712-13
CAACGCA3405300.046.17600312-13
ATCAACG3399600.046.17070410-11
AACGCAG3416350.046.05475214-15
ACGCAGA3417950.046.0512614-15
CGCAGAG3425600.045.92873816-17
AGAGTAC3343900.045.86298818-19
TATCAAC3418700.045.84395210-11
CAGAGTA3395400.045.81299218-19
GAGTACT1940000.045.68820620-21
GCAGAGT3429650.045.6376516-17
Produced by FastQC (version 0.11.5)