##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR578603_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 9419489 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.656170095851273 31.0 31.0 34.0 27.0 34.0 2 30.9511441650391 31.0 31.0 34.0 27.0 34.0 3 31.081089749136073 31.0 31.0 34.0 27.0 34.0 4 34.51628363279579 37.0 35.0 37.0 30.0 37.0 5 34.55012304807617 37.0 35.0 37.0 30.0 37.0 6 34.56790193183516 37.0 35.0 37.0 30.0 37.0 7 34.43427387621558 37.0 35.0 37.0 30.0 37.0 8 34.42187840550586 37.0 35.0 37.0 30.0 37.0 9 35.85427319889646 39.0 35.0 39.0 30.0 39.0 10-11 35.79372166579312 39.0 35.0 39.0 30.0 39.0 12-13 35.907655500208136 39.0 35.0 39.0 30.0 39.0 14-15 36.967235165304615 40.0 36.5 41.0 31.0 41.0 16-17 36.919368184410004 40.0 36.5 41.0 30.5 41.0 18-19 36.85675045642073 40.0 36.0 41.0 30.0 41.0 20-21 36.689720960447005 39.5 36.0 41.0 30.0 41.0 22-23 36.35925956280643 39.0 36.0 40.5 28.5 41.0 24-25 36.272997187002396 39.0 36.0 40.0 29.0 41.0 26-27 36.24764135294388 39.0 36.0 40.5 29.0 41.0 28-29 35.98514362084822 39.0 35.0 41.0 27.0 41.0 30-31 35.66566583388972 39.0 34.5 41.0 26.5 41.0 32-33 35.28910506716447 38.0 34.0 40.0 25.0 41.0 34-35 35.09109729837786 38.0 34.0 40.0 25.0 41.0 36-37 35.03862253037293 38.0 34.0 40.0 25.0 41.0 38-39 34.111890305302126 36.0 33.0 40.0 24.5 41.0 40-41 34.04513487939738 36.0 33.0 40.0 24.0 41.0 42-43 33.83337121578464 36.0 33.0 40.0 23.0 41.0 44-45 33.66578463014289 36.0 33.0 40.0 22.5 41.0 46-47 33.461884185012586 35.0 33.0 40.0 22.5 41.0 48-49 33.09794958091676 35.0 33.0 40.0 20.5 41.0 50-51 31.771581292785626 35.0 30.5 38.5 15.5 40.0 52-53 31.27581724443863 35.0 30.0 39.0 7.0 40.0 54-55 30.650249817160997 35.0 29.0 39.0 2.0 41.0 56-57 29.979392831182246 35.0 27.5 39.0 2.0 41.0 58-59 29.694381138934396 35.0 27.0 39.0 2.0 41.0 60-61 29.43983277649138 35.0 27.0 39.0 2.0 41.0 62-63 29.112193294137292 35.0 26.0 38.5 2.0 40.5 64-65 28.78354728159882 35.0 26.0 38.0 2.0 40.0 66-67 28.481929911484585 35.0 26.0 37.5 2.0 40.0 68-69 28.142510596912423 34.5 26.0 37.0 2.0 40.0 70-71 27.757093670367894 34.0 25.0 36.5 2.0 39.0 72-73 27.379371163340174 34.0 24.0 36.0 2.0 39.0 74-75 26.990094579440562 34.0 23.5 35.5 2.0 38.5 76-77 26.595061844649962 34.0 22.0 35.0 2.0 37.5 78-79 26.225457930892006 33.0 20.0 35.0 2.0 37.0 80-81 25.854646626796846 33.0 19.5 35.0 2.0 36.5 82-83 25.496867452151598 33.0 18.0 35.0 2.0 36.0 84-85 25.096805622895253 33.0 14.5 35.0 2.0 36.0 86-87 24.742945769138856 32.0 8.5 35.0 2.0 35.0 88-89 24.357558833605516 32.0 4.5 35.0 2.0 35.0 90-91 23.968561458057863 32.0 2.0 35.0 2.0 35.0 92-93 23.569839138832265 31.5 2.0 35.0 2.0 35.0 94-95 23.122797797205347 31.0 2.0 35.0 2.0 35.0 96-97 22.628518436615828 31.0 2.0 34.5 2.0 35.0 98-99 22.014215792385343 31.0 2.0 34.0 2.0 35.0 100-101 20.47278116679153 28.0 2.0 33.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.5190553609308708 1101 2 0.5738015854417711 1101 3 0.5640547950507262 1101 4 0.6914648648374992 1101 5 0.6389668432570446 1101 6 0.5069619909393381 1101 7 0.5691987639202978 1101 8 0.6400999684844777 1101 9 0.617722322212849 1101 10-11 0.7464778959909353 1101 12-13 0.6714187573976744 1101 14-15 0.8742766766231256 1101 16-17 0.8151146124825175 1101 18-19 0.765848064698254 1101 20-21 0.7580814682007215 1101 22-23 0.8508959766071769 1101 24-25 1.0145013520614654 1101 26-27 0.9805920100216667 1101 28-29 0.9864362526516359 1101 30-31 1.0925378619269281 1101 32-33 1.123160061314465 1101 34-35 1.113424484860694 1101 36-37 1.0387204931511818 1101 38-39 1.1341190773643675 1101 40-41 1.0774114351161828 1101 42-43 1.3458588718949613 1101 44-45 1.2618673906835554 1101 46-47 1.2416356759965197 1101 48-49 1.2389160660630694 1101 50-51 1.3226123382179829 1101 52-53 1.4506621288715884 1101 54-55 1.2749751047596796 1101 56-57 1.2435300572152848 1101 58-59 1.3312677286877452 1101 60-61 1.337791942300214 1101 62-63 1.3445299640829624 1101 64-65 1.3286513961907431 1101 66-67 1.192606134441455 1101 68-69 1.2862900586786985 1101 70-71 1.2449662997617459 1101 72-73 1.3780208745069125 1101 74-75 1.3835560158059188 1101 76-77 1.3465171120722168 1101 78-79 1.4163310820227544 1101 80-81 1.4858349597227622 1101 82-83 1.536541874939175 1101 84-85 1.6204892874815258 1101 86-87 1.5773799695436495 1101 88-89 1.7317738024217988 1101 90-91 1.8361588753837772 1101 92-93 1.8310851637784538 1101 94-95 1.9053347630234612 1101 96-97 2.1830623475827693 1101 98-99 2.310467771220001 1101 100-101 2.668886105735936 1102 1 0.45139099181243836 1102 2 0.3992466698425723 1102 3 0.4254551104827655 1102 4 0.48169294108672034 1102 5 0.3971738026950007 1102 6 0.3137572870852594 1102 7 0.3831124380041615 1102 8 0.5279338036415098 1102 9 0.4499957496862095 1102 10-11 0.6137688042393776 1102 12-13 0.5436065994508539 1102 14-15 0.7273820604745396 1102 16-17 0.7206576880148532 1102 18-19 0.6040452411968218 1102 20-21 0.6610724554820209 1102 22-23 0.693203932873331 1102 24-25 0.7807468234085562 1102 26-27 0.7587141140442952 1102 28-29 0.6881263668995743 1102 30-31 0.8237959269506874 1102 32-33 0.8137266937102439 1102 34-35 0.8719621102729036 1102 36-37 0.7779372066780397 1102 38-39 0.9062688236548908 1102 40-41 0.9088668200167049 1102 42-43 1.0756484906138652 1102 44-45 0.9933042631853581 1102 46-47 0.9584759701666243 1102 48-49 1.1443292488143229 1102 50-51 0.9498315748514621 1102 52-53 1.0076199494530051 1102 54-55 0.9396054075674449 1102 56-57 0.7786447335477398 1102 58-59 0.9019382097390967 1102 60-61 0.9148249839813083 1102 62-63 0.9104370104220969 1102 64-65 0.891213643311815 1102 66-67 0.892774013703022 1102 68-69 1.0036523718936223 1102 70-71 0.9557256534407976 1102 72-73 0.9004174017819899 1102 74-75 1.0488209638575476 1102 76-77 0.875325835936291 1102 78-79 0.9114571630419341 1102 80-81 1.0715526768970243 1102 82-83 1.1956927044345207 1102 84-85 1.2328039230735897 1102 86-87 1.2680661566277216 1102 88-89 1.3481400961302938 1102 90-91 1.3196460780051495 1102 92-93 1.2792391913070666 1102 94-95 1.3284878288690258 1102 96-97 1.6037594497694627 1102 98-99 1.751883198189791 1102 100-101 2.087365588665193 1103 1 0.40495843689479827 1103 2 0.37238741475782433 1103 3 0.208758569977622 1103 4 0.4254769649753598 1103 5 0.3884651221924429 1103 6 0.34177392511739413 1103 7 0.5489462898899333 1103 8 0.4147296322622154 1103 9 0.6409750245477 1103 10-11 0.5064615524629303 1103 12-13 0.3447917037549004 1103 14-15 0.634977516520209 1103 16-17 0.5609723678103506 1103 18-19 0.5363168791832962 1103 20-21 0.6600401121598694 1103 22-23 0.6789571775765921 1103 24-25 0.6518764980446292 1103 26-27 0.7998454323937523 1103 28-29 0.6995674926084305 1103 30-31 0.6267748639377615 1103 32-33 0.8373442459601108 1103 34-35 0.8642822182361769 1103 36-37 0.7470112937974491 1103 38-39 0.5653570584502177 1103 40-41 0.6618367883295662 1103 42-43 0.5119870473030588 1103 44-45 0.5622882722023022 1103 46-47 0.7344306936901219 1103 48-49 0.7913495943333118 1103 50-51 0.6685104858782971 1103 52-53 0.6067544560846052 1103 54-55 0.5768552141702159 1103 56-57 0.3725134217209636 1103 58-59 0.4453813913942106 1103 60-61 0.3763897795542377 1103 62-63 0.4135419654352255 1103 64-65 0.4813187170375741 1103 66-67 0.3878081729371061 1103 68-69 0.6034750204376991 1103 70-71 0.6398119966611944 1103 72-73 0.5676916879733653 1103 74-75 0.7226402623572064 1103 76-77 0.6676468570736667 1103 78-79 0.7512336245444651 1103 80-81 0.7005559343194996 1103 82-83 0.7723034130967577 1103 84-85 0.7361807756755496 1103 86-87 0.920581983891708 1103 88-89 0.9910903268372095 1103 90-91 0.8701599993443345 1103 92-93 0.9800900086631152 1103 94-95 1.0767619624298668 1103 96-97 1.1618934176283418 1103 98-99 1.3674066361076456 1103 100-101 1.6551864095565278 1104 1 0.23387896651649243 1104 2 0.30775642819469695 1104 3 0.30787276723341606 1104 4 0.2933915440382222 1104 5 0.15823203388212193 1104 6 0.22065428491502104 1104 7 0.4225868125884631 1104 8 0.389120788184421 1104 9 0.4368144769981228 1104 10-11 0.5188969223159248 1104 12-13 0.3687439946619264 1104 14-15 0.5356621795263834 1104 16-17 0.6318872516988918 1104 18-19 0.7397369651286354 1104 20-21 0.5519440843225709 1104 22-23 0.49105788500465053 1104 24-25 0.5619526657142515 1104 26-27 0.5927386672012247 1104 28-29 0.6700357616765018 1104 30-31 0.5970369236035182 1104 32-33 0.8386351697756496 1104 34-35 0.7461813855195985 1104 36-37 0.8327944051634475 1104 38-39 0.6075227157047465 1104 40-41 0.7115146870473623 1104 42-43 0.7964360619798967 1104 44-45 1.0667071788856006 1104 46-47 0.873300455629014 1104 48-49 0.8740918112684497 1104 50-51 0.9224003714782576 1104 52-53 0.8168056629230342 1104 54-55 0.7281716800523448 1104 56-57 0.8220379065849492 1104 58-59 0.8894666108364042 1104 60-61 0.8586812807302735 1104 62-63 0.6526462235657391 1104 64-65 0.8391889672142092 1104 66-67 0.6644699572264372 1104 68-69 0.701091244913389 1104 70-71 0.8297058630778018 1104 72-73 0.7106911274041146 1104 74-75 0.8337080821948994 1104 76-77 0.8897367083161534 1104 78-79 1.004994572758978 1104 80-81 0.8828279318188947 1104 82-83 0.8828412754163004 1104 84-85 0.8843716482132109 1104 86-87 0.9741668177810894 1104 88-89 1.0805766826591885 1104 90-91 1.1032900294523245 1104 92-93 1.13209578743459 1104 94-95 1.216117540287641 1104 96-97 1.4019822239387487 1104 98-99 1.6538425716964333 1104 100-101 1.8399595598394605 1105 1 0.04099362594144296 1105 2 0.22884502328841094 1105 3 0.2684126229326971 1105 4 0.30352347521092327 1105 5 0.24732716589997494 1105 6 0.3078136013537218 1105 7 0.4082477601227765 1105 8 0.40059083746710655 1105 9 0.31401122422545313 1105 10-11 0.40331114928628153 1105 12-13 0.319610259453583 1105 14-15 0.2479252415399884 1105 16-17 0.4903761203981247 1105 18-19 0.3196460028529273 1105 20-21 0.25626642929348264 1105 22-23 0.4039703912432202 1105 24-25 0.5751648721696014 1105 26-27 0.5445163099714279 1105 28-29 0.6710009797659922 1105 30-31 0.5773681458757878 1105 32-33 0.6946368958560996 1105 34-35 0.6235998255132174 1105 36-37 0.8082605286520916 1105 38-39 0.6676105849790517 1105 40-41 0.6078683851298479 1105 42-43 0.8701397003110785 1105 44-45 0.9548721388376649 1105 46-47 0.7576592594078022 1105 48-49 0.9055856479513338 1105 50-51 0.8117185365015338 1105 52-53 0.6982316177235326 1105 54-55 0.9163489849593276 1105 56-57 0.9721546180512597 1105 58-59 0.9016011851082162 1105 60-61 0.8887543102375446 1105 62-63 0.9403781817645402 1105 64-65 1.0486334848184953 1105 66-67 0.9427086112722343 1105 68-69 1.0649844281005976 1105 70-71 1.0886793788459563 1105 72-73 0.9629055498034695 1105 74-75 0.9540997652364567 1105 76-77 0.8738458987047437 1105 78-79 1.001635444740689 1105 80-81 0.9505547337857756 1105 82-83 1.135439341266796 1105 84-85 1.1968372431066285 1105 86-87 1.2196425116837517 1105 88-89 1.0995969813800421 1105 90-91 1.3302507347476364 1105 92-93 1.3661043676700935 1105 94-95 1.4723925030099352 1105 96-97 1.6033524309392995 1105 98-99 1.8656181370959857 1105 100-101 2.1775152765925867 1106 1 0.2696841951179145 1106 2 0.2874125843895534 1106 3 0.2995252419178982 1106 4 0.17927546202892586 1106 5 0.2954880510148499 1106 6 0.2467285578864491 1106 7 0.3953795157616753 1106 8 0.34728802956566796 1106 9 0.44455226694383754 1106 10-11 0.21495526502860685 1106 12-13 0.44923661240307666 1106 14-15 0.29871137873001885 1106 16-17 0.38540170510741945 1106 18-19 0.3130664141111552 1106 20-21 0.32127658985772456 1106 22-23 0.4717181729683375 1106 24-25 0.5812824941042649 1106 26-27 0.3856960446847921 1106 28-29 0.47197981731230954 1106 30-31 0.5025192872562059 1106 32-33 0.5531092047675514 1106 34-35 0.44955093377812716 1106 36-37 0.5910184393771161 1106 38-39 0.6211063322239951 1106 40-41 0.5166365917850797 1106 42-43 0.6775221778943035 1106 44-45 0.7121692528911083 1106 46-47 0.7298419134627991 1106 48-49 0.776883363891848 1106 50-51 0.7022060244383148 1106 52-53 0.6519103480141872 1106 54-55 0.8927277135094336 1106 56-57 0.7318355169029402 1106 58-59 0.8909445194705441 1106 60-61 0.7118368503813102 1106 62-63 0.8156089509814386 1106 64-65 0.921278953408855 1106 66-67 0.9089846736177805 1106 68-69 0.9054836805215132 1106 70-71 1.0050560367747146 1106 72-73 0.9427401688429633 1106 74-75 0.8520968629395718 1106 76-77 0.8681896122901946 1106 78-79 0.9990510459527222 1106 80-81 1.013054761968231 1106 82-83 1.2030732207806238 1106 84-85 1.1718969425653185 1106 86-87 1.2562223466274531 1106 88-89 1.3217471467005808 1106 90-91 1.1966847901921298 1106 92-93 1.3916278872141525 1106 94-95 1.3836025728672539 1106 96-97 1.6655539956472545 1106 98-99 1.7105368945175528 1106 100-101 1.927640654699676 1107 1 0.15765276997180422 1107 2 0.19836578605926292 1107 3 0.2264121408407327 1107 4 0.13827409359979015 1107 5 0.3199923566856313 1107 6 0.2970211178133013 1107 7 0.2661175279114829 1107 8 0.3791769488096932 1107 9 0.26968727979945584 1107 10-11 0.31790301313086644 1107 12-13 0.38082255983641744 1107 14-15 0.39028468867421395 1107 16-17 0.3006341976134834 1107 18-19 0.32186010903803464 1107 20-21 0.31577744924400264 1107 22-23 0.5189059361387365 1107 24-25 0.5221097154690924 1107 26-27 0.46369986356573634 1107 28-29 0.648926179840501 1107 30-31 0.6011893730384585 1107 32-33 0.5538398927409531 1107 34-35 0.4661346669855604 1107 36-37 0.4344430765413918 1107 38-39 0.5062737982747123 1107 40-41 0.43266763891309523 1107 42-43 0.4634152015861943 1107 44-45 0.4533107485769605 1107 46-47 0.5419905553717044 1107 48-49 0.5541973432491147 1107 50-51 0.4484144767759908 1107 52-53 0.5476904808289014 1107 54-55 0.6671997700048315 1107 56-57 0.47028832704596013 1107 58-59 0.5162074679633157 1107 60-61 0.5173207942064764 1107 62-63 0.6283401667964377 1107 64-65 0.6832382590511585 1107 66-67 0.7163136556565881 1107 68-69 0.7222544665324122 1107 70-71 0.720568442918438 1107 72-73 0.7260040287586733 1107 74-75 0.7214301179018214 1107 76-77 0.8057698856286706 1107 78-79 0.7655320233190466 1107 80-81 0.8775483849763894 1107 82-83 0.9722813004915025 1107 84-85 1.036534173404565 1107 86-87 1.081839450896183 1107 88-89 1.1647878838953396 1107 90-91 1.1984895150399808 1107 92-93 1.2144261106100416 1107 94-95 1.2105704151214312 1107 96-97 1.2447190528896535 1107 98-99 1.3944305532903556 1107 100-101 1.6405809052543 1108 1 0.18943439436788267 1108 2 0.20469700299691596 1108 3 0.005840219478443487 1108 4 0.2062358602182499 1108 5 0.27367328432177374 1108 6 0.25880042577338 1108 7 0.23164348085926179 1108 8 0.17339977911058924 1108 9 0.2000791911751847 1108 10-11 0.2609890168760032 1108 12-13 0.21987571516579152 1108 14-15 0.1993340496022995 1108 16-17 0.298445750876688 1108 18-19 0.09858815533558385 1108 20-21 0.12510349446773006 1108 22-23 0.29048010810020486 1108 24-25 0.3613276485668777 1108 26-27 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0.30191798861337205 2311 80-81 0.3039003256128332 2311 82-83 0.292873911928325 2311 84-85 0.26938716208617564 2311 86-87 0.2848844828527106 2311 88-89 0.27644866367782583 2311 90-91 0.36418368981396654 2311 92-93 0.4236228620438567 2311 94-95 0.3807721593682736 2311 96-97 0.3450705386370778 2311 98-99 0.28421741839466463 2311 100-101 0.1619453073799022 2312 1 -0.5703240724345129 2312 2 -0.14182824858139043 2312 3 -0.18733949400818162 2312 4 -0.09017316350816884 2312 5 -0.12632312845303062 2312 6 -0.040524491215713 2312 7 0.02327031791502776 2312 8 -0.09015561633651714 2312 9 -0.19060421735820654 2312 10-11 -0.30035682048271894 2312 12-13 -0.22062705346397848 2312 14-15 -0.036697775346723915 2312 16-17 0.09701925244795717 2312 18-19 -0.1389298663746743 2312 20-21 -0.25244233087114765 2312 22-23 -0.11675029832071004 2312 24-25 -0.031187483174115016 2312 26-27 -0.058955177012350646 2312 28-29 -0.07613847873346202 2312 30-31 -0.614114624938459 2312 32-33 -0.06527643268611882 2312 34-35 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0.03448494398451274 2312 98-99 -0.07645153630400614 2312 100-101 -0.010841830214559423 2313 1 -0.42390981934133265 2313 2 -0.25653235577125244 2313 3 -0.2722961397166088 2313 4 -1.186574702850379 2313 5 -0.9748054394598142 2313 6 -0.5078781766405029 2313 7 -0.34805275002506164 2313 8 -0.23472873535084915 2313 9 -0.18423824209988027 2313 10-11 -0.31725151191529477 2313 12-13 -0.743810329645548 2313 14-15 -0.9301731905639627 2313 16-17 -0.5828632652321275 2313 18-19 -0.6058166862673815 2313 20-21 -0.5048204482449279 2313 22-23 -0.5821147591032485 2313 24-25 -0.44569947669922527 2313 26-27 -0.48664224230662967 2313 28-29 -0.4022792190372897 2313 30-31 -0.6897295327061315 2313 32-33 -0.5794737060639221 2313 34-35 -0.5554309633118137 2313 36-37 -0.733738774509483 2313 38-39 -0.49972496291003665 2313 40-41 -0.33946286172676565 2313 42-43 -0.3615147832219847 2313 44-45 -0.21716022688837455 2313 46-47 -0.3803993874246956 2313 48-49 -0.5317928482421408 2313 50-51 -0.4410612232800837 2313 52-53 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2315 30-31 -2.603414443726514 2315 32-33 -2.3960166954097275 2315 34-35 -1.9150371955103864 2315 36-37 -1.898183704011565 2315 38-39 -2.058047574242515 2315 40-41 -2.290954352058904 2315 42-43 -2.265897551104345 2315 44-45 -2.4164903771547657 2315 46-47 -2.913941716250701 2315 48-49 -4.281650709034064 2315 50-51 -3.5607208068680087 2315 52-53 -3.0313463196691153 2315 54-55 -2.526293305830361 2315 56-57 -2.3727559733259618 2315 58-59 -2.6952113306779353 2315 60-61 -2.5446690528505336 2315 62-63 -2.208160278680811 2315 64-65 -1.8897174029418125 2315 66-67 -1.7098762832568077 2315 68-69 -1.6789015018164655 2315 70-71 -1.8795755062079529 2315 72-73 -1.8003730368210604 2315 74-75 -1.9504913396954215 2315 76-77 -1.9602174365859568 2315 78-79 -1.8736551006223472 2315 80-81 -1.828700558785716 2315 82-83 -1.8778087846795124 2315 84-85 -1.7691245419212045 2315 86-87 -1.7614554571001264 2315 88-89 -1.9290833630250255 2315 90-91 -1.9633275858673898 2315 92-93 -1.9080972027165295 2315 94-95 -2.0230384410968973 2315 96-97 -1.915291429411898 2315 98-99 -2.130583514885842 2315 100-101 -2.1722628807403694 2316 1 -1.2590533418544254 2316 2 -1.1036948136924103 2316 3 -2.3400959236472225 2316 4 -1.4637197113868652 2316 5 -1.2281988596024576 2316 6 -2.7224968353616923 2316 7 -6.86780994476586 2316 8 -7.642721420524364 2316 9 -4.323356370675096 2316 10-11 -2.2436556813787334 2316 12-13 -2.136300512084773 2316 14-15 -2.2589433501363914 2316 16-17 -2.1850798867981993 2316 18-19 -1.747100702424298 2316 20-21 -2.872585732449089 2316 22-23 -3.345795222215159 2316 24-25 -2.0524574686516175 2316 26-27 -2.4655498891254837 2316 28-29 -3.209523066281811 2316 30-31 -3.8035572997253446 2316 32-33 -3.7916156814116064 2316 34-35 -2.967817624973982 2316 36-37 -4.023673375184536 2316 38-39 -3.5816084361115017 2316 40-41 -4.359885141669658 2316 42-43 -3.718843635344765 2316 44-45 -3.363180738207326 2316 46-47 -2.9908578799959358 2316 48-49 -3.10684934032291 2316 50-51 -2.7736514729240938 2316 52-53 -3.1080882321090613 2316 54-55 -4.564789998536664 2316 56-57 -3.1458802345107237 2316 58-59 -2.640297752755391 2316 60-61 -3.340582645645398 2316 62-63 -2.986187829737407 2316 64-65 -4.390250992405932 2316 66-67 -3.3264524984275887 2316 68-69 -3.2454775126971214 2316 70-71 -3.7120950293980712 2316 72-73 -3.3815931522400753 2316 74-75 -3.218722705289924 2316 76-77 -3.666704743059878 2316 78-79 -4.838813289003664 2316 80-81 -3.8849788437750163 2316 82-83 -3.473484273247518 2316 84-85 -3.5678589891096735 2316 86-87 -4.690824385291165 2316 88-89 -5.131934583102854 2316 90-91 -4.523690643740821 2316 92-93 -4.5814100808881975 2316 94-95 -4.671855478697232 2316 96-97 -4.842471249992872 2316 98-99 -4.9122740042326 2316 100-101 -4.959244892913123 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 89929.0 3 16769.0 4 15054.0 5 14629.0 6 16920.0 7 21178.0 8 27544.0 9 32654.0 10 38403.0 11 46853.0 12 47789.0 13 53196.0 14 60757.0 15 71279.0 16 89067.0 17 115853.0 18 164037.0 19 243147.0 20 345622.0 21 256528.0 22 146006.0 23 133220.0 24 141398.0 25 148553.0 26 159724.0 27 178445.0 28 203642.0 29 236432.0 30 276599.0 31 328012.0 32 396756.0 33 491159.0 34 635064.0 35 899793.0 36 1295733.0 37 1141266.0 38 741856.0 39 98567.0 40 56.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 10.323200297960007 53.72949585107272 19.926022329568514 16.021281521398762 2 15.344381008213432 54.829224349749104 15.772689818653198 14.053704823384258 3 51.24092996523959 17.691153546458516 16.15672407922329 14.9111924090786 4 15.00270647584117 16.608867592231107 17.143117634895027 51.2453082970327 5 15.149777206345785 52.60314866395538 17.990044288813177 14.257029840885663 6 51.10654305428388 17.352333861814486 16.389794041108473 15.15132904279316 7 15.806704422946849 16.950125686012907 53.07960220416764 14.1635676868726 8 50.76556277710408 17.54275228983445 17.065424509315115 14.626260423746357 9 50.344225666509544 17.333094754075645 17.619740506805247 14.702939072609558 10-11 14.732121299565193 35.21356290127593 35.05987221855643 14.994443580602452 12-13 14.551522239473385 17.76416417845675 34.89358184995546 32.79073173211441 14-15 14.78908143240248 53.20910538645269 16.92370752939779 15.078105651747038 16-17 32.67248791530603 17.49801751155192 17.051875942143475 32.777618630998575 18-19 14.996385872306062 35.271628956851046 17.03546136734117 32.69652380350172 20-21 32.819517665912315 35.29401954615516 17.128387473800537 14.758075314131979 22-23 32.68925837103695 17.482968381006923 34.94850138906761 14.879271858888513 24-25 14.827900746194306 34.99975677043824 17.700139161297738 32.47220332206972 26-27 15.077701499406098 24.489765030716775 45.36546360817498 15.067069861702148 28-29 29.615290514973054 17.253411566812783 38.2300739171703 14.90122400104386 30-31 26.148047919343774 19.26948601155911 38.80631340927999 15.776152659817127 32-33 19.045844988101422 21.8949979768514 40.89968958915187 18.159467445895313 34-35 18.389829810796133 20.840479987465194 41.69684624812277 19.072843953615905 36-37 18.643071427903777 20.626327180399663 41.817956734874926 18.912644656821634 38-39 18.80308929727415 20.64958603499377 41.67579309944492 18.871531568287164 40-41 18.736733179924 20.705804387801578 41.307513630090895 19.249948802183532 42-43 19.14567223773064 20.819816721434126 41.35194230287591 18.682568737959322 44-45 18.56512202007748 20.92531051226531 41.242400630658175 19.267166836999035 46-47 19.08126446595375 20.742619658460768 41.0276235424248 19.148492333160682 48-49 18.888387146009496 21.02297412962849 41.106284100319286 18.982354624042728 50-51 19.107791122394264 20.699440547192356 40.84212615965385 19.35064217075953 52-53 19.516396189331125 21.70044646152555 39.48606208401464 19.297095265128682 54-55 20.017016265185234 22.537300283124694 37.824143203007885 19.621540248682184 56-57 20.398620382444193 23.387173949686847 36.542541943116106 19.67166372475285 58-59 20.575165041210713 24.3045624062551 35.44794229496384 19.672330257570344 60-61 20.449063647956546 24.544324993251628 35.01035635274338 19.99625500604844 62-63 20.293976186697783 24.68179072871543 34.88046293328031 20.143770151306477 64-65 20.119770693536065 24.238796179301918 35.157831946725715 20.4836011804363 66-67 20.168370907963535 23.662959945607593 36.030207053222874 20.138462093206 68-69 19.968537845128623 23.627060830620856 36.33155607508051 20.07284524917001 70-71 19.97685435605406 23.51746021832489 36.691745982532375 19.813939443088678 72-73 19.593678060477142 23.3091050698795 36.796971846771676 20.30024502287169 74-75 20.157750396479006 23.460633058927325 36.179118706952956 20.202497837640713 76-77 20.538477155074514 23.978219779894154 35.38272014952341 20.10058291550792 78-79 20.289714301615906 24.080125467417325 35.14277802926681 20.487382201699962 80-81 20.168149699552874 24.794462428083953 34.630371726811944 20.407016145551225 82-83 21.164559744854124 24.792247915469652 33.812553168666675 20.23063917100955 84-85 20.890339318124184 25.35020386703435 33.43210119133761 20.327355623503855 86-87 20.64141302693957 25.548089380603116 33.35186821223127 20.458629380226046 88-89 20.469253016706197 25.15366853353188 33.321823417138695 21.05525503262323 90-91 20.482340838346044 25.327984080542127 33.22428499024364 20.96539009086819 92-93 20.782295724462468 25.436776964810647 33.33527336651817 20.445653944208715 94-95 20.587891829509267 25.310548937694893 32.699307037739146 21.402252195056697 96-97 20.558425175142723 25.047430229375934 33.14350621884247 21.25063837663887 98-99 20.905967008101022 25.466509104724445 32.49857363255234 21.128950254622186 100-101 21.510940921186535 24.955274616641276 32.241135799351646 21.292648662820547 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 25941.0 1 15402.5 2 3393.0 3 1703.5 4 1327.0 5 1068.5 6 1190.0 7 1472.0 8 1319.0 9 1388.5 10 2323.5 11 17224.0 12 346991.0 13 377081.0 14 77695.5 15 63821.5 16 66022.0 17 69781.5 18 72702.5 19 73906.5 20 73675.0 21 73271.5 22 72815.5 23 73434.0 24 75446.5 25 78151.5 26 82334.5 27 88178.5 28 92513.5 29 96260.5 30 102324.5 31 109562.0 32 116615.0 33 125168.5 34 141122.0 35 151649.5 36 157299.5 37 167228.5 38 176353.5 39 185943.0 40 197555.0 41 210604.5 42 221622.5 43 231549.0 44 243619.5 45 253712.0 46 258862.0 47 274803.5 48 285996.0 49 286367.5 50 287953.0 51 285759.0 52 288322.5 53 293814.5 54 283456.5 55 263870.5 56 252702.0 57 241483.5 58 243941.5 59 217353.5 60 164892.5 61 134946.0 62 109125.0 63 85965.0 64 67966.5 65 55559.5 66 44612.5 67 35587.5 68 28028.0 69 21729.0 70 17493.0 71 14661.0 72 12656.5 73 10307.0 74 8132.0 75 6420.5 76 5103.0 77 4054.0 78 3222.5 79 2693.5 80 2317.5 81 2042.5 82 1841.0 83 1625.5 84 1451.0 85 1245.0 86 1028.5 87 822.5 88 613.0 89 497.5 90 384.0 91 286.0 92 214.5 93 154.0 94 112.5 95 78.5 96 58.0 97 39.5 98 24.5 99 14.5 100 4.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.12224654649525044 2 0.11030322345511524 3 0.10545158022903366 4 0.09264833793000873 5 0.09779723719620034 6 0.08749943866381711 7 0.09696916679875098 8 0.09305175684158663 9 0.08727649663373459 10-11 0.07858706560408957 12-13 0.07004095445092616 14-15 0.0775201287458375 16-17 0.06842727880461456 18-19 0.06091094750468948 20-21 0.057460654181983754 22-23 0.05011418347640726 24-25 0.04793253646774257 26-27 0.04453001643719739 28-29 0.04696114619381157 30-31 0.03848934905067568 32-33 0.04998678803064582 34-35 0.06052876116740515 36-37 0.050883864294549316 38-39 0.05292749957030578 40-41 0.05334684291260386 42-43 0.048102397062091164 44-45 0.04844211825078834 46-47 0.04771490258123344 48-49 0.04808647263137098 50-51 0.049519671396187195 52-53 0.04611715136564202 54-55 0.04649402955935295 56-57 0.046881524040210676 58-59 0.049296729366104686 60-61 0.04515637737885781 62-63 0.054005052715704646 64-65 0.049524979539760594 66-67 0.04463617930866526 68-69 0.042995962944486696 70-71 0.05051760238798517 72-73 0.045071447081683515 74-75 0.04698237876810515 76-77 0.0421838169777575 78-79 0.04729555923893536 80-81 0.048882694167379996 82-83 0.03926964615596451 84-85 0.049381659663278975 86-87 0.051027184171030936 88-89 0.04691337290165103 90-91 0.043452463291798527 92-93 0.04079308336152842 94-95 0.04443446985287631 96-97 0.032124884906176966 98-99 0.03276717027855758 100-101 0.02439091971974276 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 9419489.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 43.387182613133454 #Duplication Level Percentage of deduplicated Percentage of total 1 89.5148923481858 38.83798980905719 2 5.709295593982197 4.954205010569276 3 1.784643935936351 2.322920170436751 4 0.8658046802430595 1.5025930307604496 5 0.4974671438977988 1.0791848908163866 6 0.3112865452743739 0.8103507710898418 7 0.2248604284387523 0.6829242329797702 8 0.15923435252668647 0.5526983945087531 9 0.1355476231145223 0.529292652916137 >10 0.7702158891667397 5.513708774688372 >50 0.009218021281529832 0.2837141482055311 >100 0.00937793994053435 0.9769377403654766 >500 0.0037296484811435828 1.1435636591453997 >1k 0.0037793771272004693 3.2590981735130984 >5k 4.972864641053249E-4 1.5428249229965716 >10k+ 1.4918593923159747E-4 36.00799361795103 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 1613875 17.133360419020608 No Hit AAGCAGTGGTATCAACGCAGAGTATTTTTTTTTTTTTTTTTTTTTTTTTT 33177 0.3522165586689469 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 21850 0.2319658741572924 No Hit AAGCAGTGGTATCAACGCAGAGTACCTTTTTTTTTTTTTTTTTTTTTTTT 9446 0.10028144838854847 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 3.184886144036051E-5 0.0 0.0 0.0 1.0616287146786837E-5 3 5.3081435733934186E-5 0.0 0.0 0.0 1.0616287146786837E-5 4 5.3081435733934186E-5 0.0 0.0 0.0 1.0616287146786837E-5 5 5.3081435733934186E-5 0.0 0.0 0.0 1.0616287146786837E-5 6 5.3081435733934186E-5 0.0 0.0 0.0 1.0616287146786837E-5 7 6.369772288072102E-5 0.0 0.0 0.0 1.0616287146786837E-5 8 6.369772288072102E-5 0.0 0.0 0.0 1.0616287146786837E-5 9 7.431401002750786E-5 0.0 0.0 0.0 1.0616287146786837E-5 10-11 7.962215360090128E-5 0.0 0.0 1.0616287146786837E-5 1.0616287146786837E-5 12-13 8.49302971742947E-5 0.0 0.0 3.184886144036051E-5 1.0616287146786837E-5 14-15 1.0616287146786837E-4 1.0616287146786837E-5 0.0 3.715700501375393E-5 1.0616287146786837E-5 16-17 1.3270358933483546E-4 1.0616287146786837E-5 0.0 4.246514858714735E-5 2.1232574293573674E-5 18-19 1.5924430720180255E-4 1.0616287146786837E-5 0.0 4.246514858714735E-5 2.1232574293573674E-5 20-21 1.698605943485894E-4 1.0616287146786837E-5 0.0 4.246514858714735E-5 2.654071786696709E-5 22-23 2.1763388650913016E-4 2.6540717866967093E-5 0.0 4.246514858714735E-5 3.184886144036051E-5 24-25 2.7602346581645775E-4 4.246514858714735E-5 0.0 4.246514858714735E-5 3.184886144036051E-5 26-27 3.3441304512378534E-4 4.246514858714735E-5 0.0 4.246514858714735E-5 3.184886144036051E-5 28-29 3.4502933227057223E-4 4.246514858714735E-5 0.0 4.777329216054077E-5 3.184886144036051E-5 30-31 3.6095376299075246E-4 4.246514858714735E-5 0.0 5.3081435733934186E-5 3.715700501375393E-5 32-33 3.874944808577196E-4 4.246514858714735E-5 0.0 5.3081435733934186E-5 6.369772288072102E-5 34-35 4.3526777301826033E-4 4.246514858714735E-5 0.0 6.900586645411444E-5 7.962215360090128E-5 36-37 4.6711663445862085E-4 4.246514858714735E-5 0.0 7.431401002750786E-5 8.49302971742947E-5 38-39 5.148899266191615E-4 4.246514858714735E-5 0.0 9.554658432108153E-5 8.49302971742947E-5 40-41 5.83895793073276E-4 4.246514858714735E-5 0.0 9.554658432108153E-5 1.0616287146786837E-4 42-43 6.475935159539971E-4 4.246514858714735E-5 0.0 9.554658432108153E-5 1.0616287146786837E-4 44-45 7.006749516879312E-4 4.246514858714735E-5 0.0 9.554658432108153E-5 1.0616287146786837E-4 46-47 7.484482438484721E-4 4.246514858714735E-5 0.0 9.554658432108153E-5 1.0616287146786837E-4 48-49 7.962215360090128E-4 4.246514858714735E-5 0.0 9.554658432108153E-5 1.0616287146786837E-4 50-51 8.599192588897339E-4 4.246514858714735E-5 0.0 9.554658432108153E-5 1.0616287146786837E-4 52-53 9.607739867842087E-4 4.246514858714735E-5 0.0 9.554658432108153E-5 1.1677915861465521E-4 54-55 0.0010350879968117166 4.246514858714735E-5 0.0 9.554658432108153E-5 1.1677915861465521E-4 56-57 0.0011359427247061916 4.246514858714735E-5 0.0 9.554658432108153E-5 1.1677915861465521E-4 58-59 0.001231489309027273 4.246514858714735E-5 0.0 9.554658432108153E-5 1.1677915861465521E-4 60-61 0.0012845707447612072 4.246514858714735E-5 0.0 9.554658432108153E-5 1.2739544576144204E-4 62-63 0.0013588847547887153 4.246514858714735E-5 0.0 9.554658432108153E-5 1.2739544576144204E-4 64-65 0.0014385069083896166 4.246514858714735E-5 0.0 9.554658432108153E-5 1.3801173290822887E-4 66-67 0.0015605942105776651 4.246514858714735E-5 0.0 1.2208730218804862E-4 1.3801173290822887E-4 68-69 0.0017251466613528612 4.246514858714735E-5 0.0 1.4862802005501571E-4 1.3801173290822887E-4 70-71 0.0018631583942610899 4.246514858714735E-5 0.0 1.4862802005501571E-4 1.3801173290822887E-4 72-73 0.001974629409302352 5.3081435733934186E-5 0.0 1.4862802005501571E-4 1.3801173290822887E-4 74-75 0.0022081877265316624 5.3081435733934186E-5 0.0 1.4862802005501571E-4 1.433198764816223E-4 76-77 0.0023515076030132843 6.369772288072102E-5 0.0 1.5393616362840913E-4 1.5393616362840913E-4 78-79 0.002574449633095808 6.369772288072102E-5 0.0 1.5924430720180255E-4 1.5924430720180255E-4 80-81 0.0029300952525131672 6.369772288072102E-5 0.0 1.5924430720180255E-4 1.5924430720180255E-4 82-83 0.003370671169104821 6.369772288072102E-5 0.0 1.698605943485894E-4 1.698605943485894E-4 84-85 0.003922718100737736 6.369772288072102E-5 0.0 1.698605943485894E-4 1.698605943485894E-4 86-87 0.004994963102563207 6.369772288072102E-5 0.0 1.698605943485894E-4 1.698605943485894E-4 88-89 0.006247684985884054 6.369772288072102E-5 0.0 1.751687379219828E-4 1.751687379219828E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AAGCAGT 338810 0.0 92.37316 1 AGTGGTA 337640 0.0 91.93445 5 GGTATCA 339870 0.0 91.59727 8 TGGTATC 339345 0.0 91.59596 7 AGCAGTG 341150 0.0 91.567726 2 GTATCAA 340285 0.0 91.5054 9 GTGGTAT 340330 0.0 91.44772 6 GCAGTGG 341610 0.0 91.32834 3 CAGTGGT 342620 0.0 90.82608 4 TCAACGC 340550 0.0 46.17957 12-13 CAACGCA 340530 0.0 46.176003 12-13 ATCAACG 339960 0.0 46.170704 10-11 AACGCAG 341635 0.0 46.054752 14-15 ACGCAGA 341795 0.0 46.05126 14-15 CGCAGAG 342560 0.0 45.928738 16-17 AGAGTAC 334390 0.0 45.862988 18-19 TATCAAC 341870 0.0 45.843952 10-11 CAGAGTA 339540 0.0 45.812992 18-19 GAGTACT 194000 0.0 45.688206 20-21 GCAGAGT 342965 0.0 45.63765 16-17 >>END_MODULE