FastQCFastQC Report
Sat 18 Jun 2016
SRR578603_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR578603_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9419489
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT210230.22318620468689968No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT203770.21632808319007538No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT109600.026.854871
AGTGGTA119750.023.4306075
GGTATCA124950.022.493268
GTATCAA128450.021.8064449
TGGTATC137150.020.5963557
AGCAGTG146450.020.2288592
ATCAACG112050.019.9136410-11
GTGGTAT142100.019.91236
CAACGCA113050.019.63250412-13
GCAGTGG153500.018.990593
ACGCAGA130350.017.0997314-15
CAGTGGT168150.017.0254614
AGAGTAC134700.015.94842918-19
TCAACGC111600.015.8676139
CGTCACG6450.015.4574573
ACTCGCG3550.015.38239950-51
CTCGCGT3000.015.03637352-53
AGTACTT108850.014.611114520-21
AACGCAG118950.013.92918212-13
GCAGAGT160700.013.64871916-17
Produced by FastQC (version 0.11.5)