FastQCFastQC Report
Sat 18 Jun 2016
SRR578602_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR578602_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9339929
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT152410116.318121904352807No Hit
AAGCAGTGGTATCAACGCAGAGTATTTTTTTTTTTTTTTTTTTTTTTTTT309750.33164063666865135No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAG236250.2529462483065985No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA218290.23371698007554448No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGAT108180.11582529160553577No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT3285800.092.376431
AGTGGTA3282000.092.041915
TGGTATC3305600.091.576097
GGTATCA3304950.091.5754248
AGCAGTG3315900.091.514052
GTGGTAT3310350.091.482916
GTATCAA3307150.091.481039
GCAGTGG3320050.091.196433
CAGTGGT3325750.090.916854
ATCAACG3306600.046.17498810-11
CAACGCA3311700.046.15452212-13
TCAACGC3310950.046.11694312-13
ACGCAGA3321600.046.0403414-15
AACGCAG3320400.045.9997914-15
CGCAGAG3328450.045.89090716-17
AGAGTAC3257100.045.8492218-19
TATCAAC3326050.045.83431610-11
CAGAGTA3305850.045.7559718-19
GAGTACT1809700.045.5976220-21
GCAGAGT3342700.045.55541616-17
Produced by FastQC (version 0.11.5)