##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR578602_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 9339929 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.080430268795403 31.0 31.0 34.0 28.0 34.0 2 31.426961382682887 33.0 31.0 34.0 28.0 34.0 3 31.565023888297222 34.0 31.0 34.0 28.0 34.0 4 34.94331851987312 37.0 35.0 37.0 32.0 37.0 5 34.968427383120364 37.0 35.0 37.0 32.0 37.0 6 35.025540772312084 37.0 35.0 37.0 32.0 37.0 7 34.934979377252226 37.0 35.0 37.0 32.0 37.0 8 34.987503973531275 37.0 35.0 37.0 32.0 37.0 9 36.4847562545711 39.0 37.0 39.0 32.0 39.0 10-11 36.36313975191889 39.0 37.0 39.0 32.0 39.0 12-13 36.456238264766256 39.0 37.0 39.0 32.0 39.0 14-15 37.74204509477535 40.0 38.0 41.0 32.0 41.0 16-17 37.70138595271977 40.0 38.0 41.0 32.0 41.0 18-19 37.598151709718564 40.0 38.0 41.0 32.0 41.0 20-21 37.48485331098341 40.0 37.5 41.0 31.5 41.0 22-23 37.212999317232494 39.5 37.5 41.0 31.5 41.0 24-25 37.18607047227019 40.0 37.0 41.0 31.0 41.0 26-27 37.09175862043491 40.0 37.0 41.0 30.0 41.0 28-29 36.94877894682069 40.0 37.0 41.0 30.0 41.0 30-31 36.68149591929446 40.0 36.5 41.0 30.0 41.0 32-33 36.38355072078171 40.0 36.0 41.0 29.0 41.0 34-35 36.225704767134744 39.0 36.0 41.0 28.5 41.0 36-37 35.899831251393884 39.0 35.0 40.5 27.5 41.0 38-39 34.82156079559063 37.0 34.5 40.0 26.5 41.0 40-41 34.58840955857373 37.0 34.0 40.0 26.0 41.0 42-43 34.279911817316815 36.5 34.0 40.0 24.0 41.0 44-45 34.00505705129022 36.0 33.0 40.0 24.0 41.0 46-47 33.85696711398984 36.0 33.0 40.0 23.0 41.0 48-49 33.859565581280116 36.0 33.0 40.0 23.0 41.0 50-51 32.95748147550158 36.0 32.0 39.5 20.5 40.5 52-53 32.44707684608737 36.0 32.0 39.5 11.5 40.5 54-55 31.76135771481775 37.0 31.0 40.0 2.0 41.0 56-57 31.091709369525187 36.0 30.0 40.0 2.0 41.0 58-59 30.83821450891115 36.0 30.0 40.0 2.0 41.0 60-61 30.6151511430119 35.5 30.0 40.0 2.0 41.0 62-63 30.29407322047095 35.0 29.0 39.0 2.0 41.0 64-65 30.00315446723417 35.0 29.0 39.0 2.0 41.0 66-67 29.676321629425665 35.0 29.0 38.5 2.0 41.0 68-69 29.34240709966853 35.0 28.5 37.5 2.0 40.0 70-71 29.049427356460633 35.0 28.0 37.0 2.0 40.0 72-73 28.735117793721987 35.0 28.0 37.0 2.0 39.0 74-75 28.376642584756265 35.0 27.0 36.0 2.0 39.0 76-77 28.014089132797476 35.0 26.5 36.0 2.0 38.5 78-79 27.688304804030096 35.0 26.0 35.0 2.0 37.0 80-81 27.387921953154034 34.0 26.0 35.0 2.0 37.0 82-83 27.140366538118222 34.0 25.0 35.0 2.0 36.5 84-85 26.879425903558797 34.0 25.0 35.0 2.0 36.0 86-87 26.650007457230135 34.0 24.5 35.0 2.0 36.0 88-89 26.404436693255377 34.0 24.0 35.0 2.0 35.5 90-91 26.168892022626725 34.0 23.0 35.0 2.0 35.0 92-93 25.86680792755491 34.0 20.0 35.0 2.0 35.0 94-95 25.593629566134815 34.0 18.5 35.0 2.0 35.0 96-97 25.314599179501258 34.0 11.5 35.0 2.0 35.0 98-99 24.99771550725921 34.0 2.0 35.0 2.0 35.0 100-101 24.161760116163627 33.0 2.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.5788319359928025 1101 2 0.6004867974675108 1101 3 0.4988944486186675 1101 4 0.6016843758648776 1101 5 0.575779237568959 1101 6 0.5165062439477452 1101 7 0.5289928860562512 1101 8 0.5173556165499562 1101 9 0.6808319501395346 1101 10-11 0.737422631291075 1101 12-13 0.7115682513472095 1101 14-15 0.8322933597502598 1101 16-17 0.8089631621433 1101 18-19 0.8165753091347838 1101 20-21 0.880030871560372 1101 22-23 0.9431034202634478 1101 24-25 0.9107827131214918 1101 26-27 0.9582191968824603 1101 28-29 1.0494708511846937 1101 30-31 1.089130791258576 1101 32-33 1.0840737136408691 1101 34-35 1.1755027707445649 1101 36-37 1.1745356754380154 1101 38-39 1.1606997533895154 1101 40-41 1.0934651972387712 1101 42-43 1.1162716224072469 1101 44-45 1.2532019881314937 1101 46-47 1.1740525435024978 1101 48-49 1.1265082731543075 1101 50-51 1.2559948211172198 1101 52-53 1.2089192628079104 1101 54-55 1.1676663215927654 1101 56-57 1.1221340026064475 1101 58-59 1.0939542451864455 1101 60-61 1.0081000275902774 1101 62-63 1.0242185463623201 1101 64-65 1.0191954032505315 1101 66-67 1.0091525755032507 1101 68-69 0.9220448528955387 1101 70-71 1.0538853023640904 1101 72-73 0.9876088473430293 1101 74-75 1.0049598259673864 1101 76-77 0.9860581179361709 1101 78-79 1.0104018992617405 1101 80-81 1.007645551512617 1101 82-83 1.0220471418486134 1101 84-85 0.9732487683893218 1101 86-87 0.9601639524546464 1101 88-89 0.9230287026579767 1101 90-91 1.0023422812315808 1101 92-93 1.0822549233983239 1101 94-95 1.115531191314652 1101 96-97 1.1463216538464103 1101 98-99 1.142935841795687 1101 100-101 1.2844331665836926 1102 1 0.5360761161155096 1102 2 0.5020457705493939 1102 3 0.5451354528887968 1102 4 0.5276927038066503 1102 5 0.5797596634593489 1102 6 0.585768689700636 1102 7 0.578520582830528 1102 8 0.5189443722385718 1102 9 0.6683983779965317 1102 10-11 0.6523410190604721 1102 12-13 0.6612038948504804 1102 14-15 0.8145309606821414 1102 16-17 0.8257653602292052 1102 18-19 0.9077272473168492 1102 20-21 0.8989474679616549 1102 22-23 0.897534161391178 1102 24-25 0.9255393400603111 1102 26-27 0.9736046123485878 1102 28-29 1.0526162143935238 1102 30-31 1.0414475620073134 1102 32-33 1.1455950463219011 1102 34-35 1.0589073021019146 1102 36-37 1.1080354045498808 1102 38-39 1.146348977775574 1102 40-41 1.1850374924651703 1102 42-43 1.2119726389176009 1102 44-45 1.2781970103932778 1102 46-47 1.3102305790091506 1102 48-49 1.2090386831510571 1102 50-51 1.2505566498317364 1102 52-53 1.0689908370629908 1102 54-55 1.0418173068619971 1102 56-57 0.9534911875425287 1102 58-59 1.046751932593594 1102 60-61 1.0154712889329964 1102 62-63 1.0666534095496516 1102 64-65 1.1298124154983178 1102 66-67 1.1113718526408967 1102 68-69 1.0102973677540632 1102 70-71 1.025411188288114 1102 72-73 0.9975315182702111 1102 74-75 0.9592307659484156 1102 76-77 1.045576015761661 1102 78-79 1.0110379406487837 1102 80-81 1.0432761955190948 1102 82-83 1.074956308003916 1102 84-85 1.067861189771012 1102 86-87 1.0158251244712488 1102 88-89 1.0686639731419092 1102 90-91 1.059907805392836 1102 92-93 1.1036136556111487 1102 94-95 1.166087536831924 1102 96-97 1.2112265426497473 1102 98-99 1.264849363955019 1102 100-101 1.31889924131465 1103 1 0.41940782557490763 1103 2 0.46587136816982877 1103 3 0.37219700056603244 1103 4 0.4330995604413559 1103 5 0.4675259130177949 1103 6 0.5353670505884196 1103 7 0.3968575468834814 1103 8 0.39299647396605053 1103 9 0.5146535111449282 1103 10-11 0.559196214670159 1103 12-13 0.5791884955290598 1103 14-15 0.64651254128907 1103 16-17 0.744374973924387 1103 18-19 0.7324444356545925 1103 20-21 0.7517286373007863 1103 22-23 0.8465574055376308 1103 24-25 0.7663645749484047 1103 26-27 0.7995652529371 1103 28-29 0.7856649910189333 1103 30-31 0.812890408592807 1103 32-33 0.9095504093719313 1103 34-35 0.9145425061506813 1103 36-37 0.9648738410437687 1103 38-39 0.7751053749380006 1103 40-41 0.9334567856025728 1103 42-43 0.8504052610609207 1103 44-45 0.7776432843486276 1103 46-47 1.047361697567247 1103 48-49 1.0799443952971401 1103 50-51 1.0933518982327044 1103 52-53 1.0303564613914062 1103 54-55 0.9005462785724099 1103 56-57 0.8888461017402172 1103 58-59 0.8650381397929294 1103 60-61 0.9215538549959703 1103 62-63 0.7590087688649447 1103 64-65 0.6946581196713595 1103 66-67 0.6680223526380686 1103 68-69 0.8292239085672186 1103 70-71 0.8237461441714125 1103 72-73 0.8866742326812336 1103 74-75 0.8843459663052755 1103 76-77 0.8743192975948766 1103 78-79 0.8553463189253137 1103 80-81 0.9211963638042384 1103 82-83 0.9161430894145184 1103 84-85 0.9691712905051908 1103 86-87 0.9788439629861223 1103 88-89 0.9941107408101963 1103 90-91 0.9285202115064735 1103 92-93 0.9832976756474388 1103 94-95 0.9596726988769859 1103 96-97 0.9663915007834447 1103 98-99 1.06616930924503 1103 100-101 1.1293160955672512 1104 1 0.3360984948837533 1104 2 0.34000883609984456 1104 3 0.3498732792975119 1104 4 0.357594293911923 1104 5 0.4365428127484421 1104 6 0.44086195684173646 1104 7 0.4034006405000028 1104 8 0.31675845373322176 1104 9 0.4672722388016197 1104 10-11 0.48820804190477674 1104 12-13 0.513365436851366 1104 14-15 0.6151531842281699 1104 16-17 0.5774612974246409 1104 18-19 0.6180436172758803 1104 20-21 0.684929445666917 1104 22-23 0.663877516008391 1104 24-25 0.6689859321542144 1104 26-27 0.6857264858012329 1104 28-29 0.762733472062024 1104 30-31 0.8657102023783381 1104 32-33 0.8462249206995054 1104 34-35 0.8963508274044685 1104 36-37 0.8342150969891975 1104 38-39 0.8859030761803837 1104 40-41 0.8582889759861487 1104 42-43 1.0536555906558291 1104 44-45 0.9983337568975941 1104 46-47 1.1468274025501515 1104 48-49 1.047600708841152 1104 50-51 1.0525068182857353 1104 52-53 0.9294611495175218 1104 54-55 0.8757057205656515 1104 56-57 0.8277222695216189 1104 58-59 0.8559394974191008 1104 60-61 0.8658162479917699 1104 62-63 0.7086613464199516 1104 64-65 0.5869542056767862 1104 66-67 0.7143012691332373 1104 68-69 0.7402497927739411 1104 70-71 0.6463050544889803 1104 72-73 0.6597673932359065 1104 74-75 0.7216164556708868 1104 76-77 0.7729327407137845 1104 78-79 0.7187379890238859 1104 80-81 0.8037623953874657 1104 82-83 0.7965797275463551 1104 84-85 0.8179508044731456 1104 86-87 0.8180530263566013 1104 88-89 0.8335713968774279 1104 90-91 0.8143487403718659 1104 92-93 0.6652987600261042 1104 94-95 0.544436514861875 1104 96-97 0.6785104084613742 1104 98-99 0.8949799173052746 1104 100-101 0.7891348869271688 1105 1 0.24453891691165808 1105 2 0.18659785111921678 1105 3 0.21142074324287208 1105 4 0.20489225919108378 1105 5 0.17899650803632738 1105 6 0.17594276610808635 1105 7 0.16099426319616583 1105 8 0.14716151395951016 1105 9 0.12612886486742525 1105 10-11 0.1679671948410757 1105 12-13 0.14436332937359708 1105 14-15 0.03833491201903172 1105 16-17 0.06847206463437061 1105 18-19 -0.036423642948754775 1105 20-21 0.04562611603297739 1105 22-23 0.02626779978894689 1105 24-25 -0.00868848825334112 1105 26-27 -0.34187177756484033 1105 28-29 -0.11326989415280764 1105 30-31 -0.27361907265523655 1105 32-33 -0.23456884479305273 1105 34-35 -0.08679222682601306 1105 36-37 -0.057303310894873505 1105 38-39 -0.02787989781244704 1105 40-41 -0.0731671790010111 1105 42-43 0.07119717618985533 1105 44-45 0.01676583673376797 1105 46-47 0.09163008909713 1105 48-49 0.1199628258828298 1105 50-51 0.06241518283836456 1105 52-53 -0.09617870329158507 1105 54-55 -0.18025836713320942 1105 56-57 -0.04550950764991413 1105 58-59 -0.04149773139062418 1105 60-61 -0.09736071489285081 1105 62-63 -0.012846753125746346 1105 64-65 -0.08013986760557756 1105 66-67 -0.02246006202015849 1105 68-69 -0.03582042095174032 1105 70-71 0.023195845974949947 1105 72-73 0.026434185286213108 1105 74-75 0.07939131017955603 1105 76-77 0.05186977768653378 1105 78-79 0.0613968701366403 1105 80-81 0.12578750086420243 1105 82-83 0.11752009241406824 1105 84-85 0.030455231622074308 1105 86-87 0.10023946649091542 1105 88-89 0.1682081341570445 1105 90-91 0.17454004090067698 1105 92-93 0.23032181594738788 1105 94-95 0.25612946808641723 1105 96-97 0.21155711150830925 1105 98-99 0.22778491428762848 1105 100-101 0.2157533353060579 1106 1 -0.11252465314987603 1106 2 -0.09584227161250425 1106 3 -0.12443963335771002 1106 4 -0.13389801220918685 1106 5 -0.1870295448237087 1106 6 -0.11707905063681778 1106 7 -0.2029789526531971 1106 8 -0.19828305833099336 1106 9 -0.10058361455747189 1106 10-11 -0.335979019451635 1106 12-13 -0.38563647512547305 1106 14-15 -0.32835129601840407 1106 16-17 -0.3165474392934229 1106 18-19 -0.3673113892433264 1106 20-21 -0.1598922920616488 1106 22-23 -0.17230590193691597 1106 24-25 -0.2781043540297006 1106 26-27 -0.23798121312873377 1106 28-29 -0.26766876910414794 1106 30-31 -0.27103616895357874 1106 32-33 -0.1643215580475328 1106 34-35 -0.20386281722748123 1106 36-37 -0.28481687687131796 1106 38-39 -0.2761524003899396 1106 40-41 -0.3204552104204197 1106 42-43 -0.3930108550904876 1106 44-45 -0.34193549407930846 1106 46-47 -0.4193994191701833 1106 48-49 -0.564893896420358 1106 50-51 -0.6916640312723175 1106 52-53 -0.7399972623306148 1106 54-55 -0.6954601393559834 1106 56-57 -0.7051176450390457 1106 58-59 -0.6397965804907315 1106 60-61 -0.7985046844062715 1106 62-63 -0.6320019117467126 1106 64-65 -0.6185787083296752 1106 66-67 -0.820753232995763 1106 68-69 -0.9266776499125982 1106 70-71 -0.7782841770510451 1106 72-73 -0.8594963579560435 1106 74-75 -0.9138939319188104 1106 76-77 -0.9645145807787863 1106 78-79 -0.8352538924235198 1106 80-81 -0.8810714255780212 1106 82-83 -0.9633928752448071 1106 84-85 -1.06038117131882 1106 86-87 -0.9634190800839022 1106 88-89 -0.9839194286916104 1106 90-91 -0.8984375440393357 1106 92-93 -0.8778176565337859 1106 94-95 -0.9220145821567947 1106 96-97 -0.8950007073681867 1106 98-99 -0.9830650845031066 1106 100-101 -1.103042916503771 1107 1 -0.4334557727791264 1107 2 -0.48206154546929625 1107 3 -0.4261287309180162 1107 4 -0.33072246796800187 1107 5 -0.384871637225757 1107 6 -0.4053670269799099 1107 7 -0.35653520652588355 1107 8 -0.3180461299202193 1107 9 -0.428569462348527 1107 10-11 -0.46157329242975464 1107 12-13 -0.484761762113159 1107 14-15 -0.6565526488202877 1107 16-17 -0.5240148083904828 1107 18-19 -0.48693444384009155 1107 20-21 -0.4272206378868404 1107 22-23 -0.5130895394391644 1107 24-25 -0.5172127882292799 1107 26-27 -0.3752160393758217 1107 28-29 -0.3982997641959969 1107 30-31 -0.40588766938881093 1107 32-33 -0.38891595653802113 1107 34-35 -0.44475816885879027 1107 36-37 -0.6417910598295933 1107 38-39 -0.6326685231865952 1107 40-41 -0.7817784034602653 1107 42-43 -0.68488733550587 1107 44-45 -0.6019597626245456 1107 46-47 -0.6618907210574392 1107 48-49 -0.7356439912232915 1107 50-51 -0.8373034695031123 1107 52-53 -0.8563130161247798 1107 54-55 -0.8094032497775814 1107 56-57 -0.7911181997806551 1107 58-59 -0.7081947658659402 1107 60-61 -0.6639119205141562 1107 62-63 -0.6958168174665289 1107 64-65 -0.7264419757586644 1107 66-67 -0.797652047721634 1107 68-69 -0.6834008069777973 1107 70-71 -0.7009539648279208 1107 72-73 -0.7804859361083913 1107 74-75 -0.8403995932172954 1107 76-77 -0.8672968155736598 1107 78-79 -0.8113031480468962 1107 80-81 -0.7603245157330427 1107 82-83 -0.8077371585813218 1107 84-85 -0.882256424956747 1107 86-87 -0.842087231822223 1107 88-89 -0.9865493181407068 1107 90-91 -0.8782203007604075 1107 92-93 -0.8824594724359933 1107 94-95 -0.833169389618515 1107 96-97 -0.9167121708428247 1107 98-99 -0.9203033294507996 1107 100-101 -0.9913684242588197 1108 1 -0.6728073115706863 1108 2 -0.7275986603230535 1108 3 -0.6082200570573377 1108 4 -0.5953254640603305 1108 5 -0.7577928316060891 1108 6 -0.6766904188245562 1108 7 -0.5850094404131099 1108 8 -0.655662666657733 1108 9 -0.7247454874206412 1108 10-11 -1.0033774762795957 1108 12-13 -0.8531277663816539 1108 14-15 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58-59 -0.5099887273692545 2305 60-61 -0.4023508319585183 2305 62-63 -0.38496218139751903 2305 64-65 -0.42707730058604554 2305 66-67 -0.3474702436083632 2305 68-69 -0.31461327302188735 2305 70-71 -0.3301581186582645 2305 72-73 -0.2126340173440333 2305 74-75 -0.25762791224787307 2305 76-77 -0.30029113953029807 2305 78-79 -0.3116578782272157 2305 80-81 -0.24892692270329064 2305 82-83 -0.29730297602234756 2305 84-85 -0.24515568331857907 2305 86-87 -0.23111916167696833 2305 88-89 -0.1970641642692712 2305 90-91 -0.1767781010768381 2305 92-93 -0.21914002745314676 2305 94-95 -0.11432285031101586 2305 96-97 -0.1279679355765282 2305 98-99 -0.12628608792041618 2305 100-101 -0.06605903817790093 2306 1 -0.3485306749752226 2306 2 -0.33929126034960433 2306 3 -0.33946580940877524 2306 4 -0.5195996238927805 2306 5 -0.47741028069869884 2306 6 -0.42571923051329463 2306 7 -0.47831187695348376 2306 8 -0.4361508267398406 2306 9 -0.6297445858789033 2306 10-11 -0.7681725191870399 2306 12-13 -0.7541698891855049 2306 14-15 -0.8354006760754658 2306 16-17 -0.7313357875026583 2306 18-19 -0.6661216693474827 2306 20-21 -0.6859916377965192 2306 22-23 -0.640547310904779 2306 24-25 -0.6649784034898687 2306 26-27 -0.7333257469728025 2306 28-29 -0.6295277102379657 2306 30-31 -0.6272402775432369 2306 32-33 -0.7717387233871094 2306 34-35 -0.6794776154415487 2306 36-37 -0.635719595588057 2306 38-39 -0.7105277237532661 2306 40-41 -0.827133005712156 2306 42-43 -0.9340874746814265 2306 44-45 -0.970099123851746 2306 46-47 -0.9167957749808977 2306 48-49 -1.001072584666197 2306 50-51 -0.948258239105634 2306 52-53 -1.0908510474324231 2306 54-55 -1.01077277313793 2306 56-57 -1.1223448457797929 2306 58-59 -1.1078304166715576 2306 60-61 -1.060968066817825 2306 62-63 -1.120220853610416 2306 64-65 -1.1514732865409982 2306 66-67 -1.1352183924248536 2306 68-69 -1.142686333819693 2306 70-71 -1.219535209777245 2306 72-73 -1.2726020379931846 2306 74-75 -1.308412211492513 2306 76-77 -1.2892834069776171 2306 78-79 -1.3032455083788221 2306 80-81 -1.4363710629176758 2306 82-83 -1.4649344191041997 2306 84-85 -1.4587976818672814 2306 86-87 -1.3967716664576066 2306 88-89 -1.3916482691564802 2306 90-91 -1.3781748695581264 2306 92-93 -1.3260359283386158 2306 94-95 -1.4153067005468678 2306 96-97 -1.3470725007664406 2306 98-99 -1.3327251430875258 2306 100-101 -1.4092576368802234 2307 1 -0.610134133970103 2307 2 -0.6420081806086841 2307 3 -0.6200929331132592 2307 4 -0.7019549136397458 2307 5 -0.6816988332563838 2307 6 -0.5937016832400488 2307 7 -0.6077678324278182 2307 8 -0.5571983159266978 2307 9 -0.7062369342911481 2307 10-11 -0.7642333043286627 2307 12-13 -0.7189528100235592 2307 14-15 -0.9245428508007407 2307 16-17 -0.8636316724370872 2307 18-19 -0.8752839505110259 2307 20-21 -0.7795357644400198 2307 22-23 -0.8090308436666263 2307 24-25 -0.8656312961239152 2307 26-27 -0.9819970658183692 2307 28-29 -0.9222589766160496 2307 30-31 -0.9355435031058263 2307 32-33 -1.1815009610883678 2307 34-35 -0.9280505973696407 2307 36-37 -1.0099032005444144 2307 38-39 -1.009599030138837 2307 40-41 -1.069503370693674 2307 42-43 -1.1878612963301407 2307 44-45 -1.2203936932073063 2307 46-47 -1.274426781032112 2307 48-49 -1.3107665616923683 2307 50-51 -1.2349400802761217 2307 52-53 -1.1317125836837931 2307 54-55 -1.0334840339684597 2307 56-57 -0.9517728887572225 2307 58-59 -1.0248656317103837 2307 60-61 -0.9820606739715139 2307 62-63 -0.9698561580023828 2307 64-65 -1.0033829063336235 2307 66-67 -0.9600134887902598 2307 68-69 -0.9116209399542363 2307 70-71 -0.9564582554214276 2307 72-73 -1.018533399266179 2307 74-75 -0.9632208052337852 2307 76-77 -0.9095565464249447 2307 78-79 -0.9307959310352487 2307 80-81 -0.9262103861687123 2307 82-83 -0.9441865140818528 2307 84-85 -0.9267119216618802 2307 86-87 -1.0170379147088653 2307 88-89 -1.01418369368767 2307 90-91 -0.9980948419630735 2307 92-93 -0.9870020444806933 2307 94-95 -0.9240804272502103 2307 96-97 -0.9998610237054955 2307 98-99 -1.1669802887481318 2307 100-101 -1.2394050531663332 2308 1 -1.6261388286355078 2308 2 -1.2490458230072967 2308 3 -1.2392128509494285 2308 4 -1.1588621029587003 2308 5 -1.4985258690459347 2308 6 -1.4884465906236812 2308 7 -1.4613542169999647 2308 8 -1.323965266792861 2308 9 -1.4380257546089865 2308 10-11 -1.5100919925359264 2308 12-13 -1.3417023064559146 2308 14-15 -1.8028661958177494 2308 16-17 -1.9654496894478868 2308 18-19 -1.7145734360190303 2308 20-21 -2.9922694899553264 2308 22-23 -3.1973550705141847 2308 24-25 -2.3506827098632 2308 26-27 -2.666428925555188 2308 28-29 -2.3178088631304377 2308 30-31 -2.170526539723568 2308 32-33 -2.537061936994739 2308 34-35 -2.6868188811849336 2308 36-37 -2.929552927621536 2308 38-39 -3.051469389963607 2308 40-41 -2.722774381833574 2308 42-43 -2.8083763048203423 2308 44-45 -2.7760950158417614 2308 46-47 -2.882203515636732 2308 48-49 -2.659088498457052 2308 50-51 -2.5874694346890337 2308 52-53 -2.542976105991311 2308 54-55 -2.2348201669117174 2308 56-57 -2.121189437758158 2308 58-59 -1.9984899746958718 2308 60-61 -1.9646630813276111 2308 62-63 -1.9877784849604723 2308 64-65 -1.9086592803649367 2308 66-67 -2.061651625570857 2308 68-69 -1.915856887084356 2308 70-71 -1.903703962811651 2308 72-73 -1.8486477827398886 2308 74-75 -1.6589595113133377 2308 76-77 -1.5738366109503659 2308 78-79 -1.6316824116800355 2308 80-81 -2.2530532225151667 2308 82-83 -2.0679949111278475 2308 84-85 -1.7890641380250507 2308 86-87 -1.712532827378272 2308 88-89 -1.644179696483949 2308 90-91 -1.8666495951528184 2308 92-93 -1.8541853324497772 2308 94-95 -1.9456081336603113 2308 96-97 -1.8450938545013962 2308 98-99 -1.850212464179922 2308 100-101 -1.9606254546961743 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 81415.0 3 14275.0 4 13047.0 5 12673.0 6 15043.0 7 18380.0 8 22637.0 9 26639.0 10 30499.0 11 37773.0 12 36281.0 13 39847.0 14 43490.0 15 51149.0 16 62980.0 17 83802.0 18 125330.0 19 208991.0 20 341898.0 21 251634.0 22 125221.0 23 111404.0 24 117517.0 25 120205.0 26 123024.0 27 134746.0 28 151411.0 29 175631.0 30 207551.0 31 246535.0 32 302635.0 33 381489.0 34 508735.0 35 779722.0 36 1347646.0 37 1503994.0 38 1267411.0 39 217052.0 40 217.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 10.412448704220512 53.213878494401115 20.143981687990543 16.22969111338783 2 15.531156995911477 54.2877513746025 15.940105728679448 14.24098590080657 3 50.734750688405164 17.829846453260085 16.333887943702134 15.101514914632617 4 15.156940315447898 16.72454909500835 17.351509047881102 50.76700154166265 5 15.3040317533798 52.07152440226663 18.20632838687001 14.418115457483562 6 50.560039178598494 17.506190983033566 16.561919520057884 15.37185031831005 7 15.914954614187671 17.070844994434044 52.68592636704825 14.328274024330037 8 50.225682382041 17.703445801376162 17.255775043784684 14.81509677279815 9 49.764387170001235 17.481210615640776 17.84732922513532 14.907072989222673 10-11 14.827535806678203 35.017598168548375 34.942199626514075 15.21266639825935 12-13 14.689108387551505 17.89484032030923 34.75673595905744 32.65931533308182 14-15 14.931568163334799 52.65456525451996 17.136758200014885 15.277108382130358 16-17 32.47312904402701 17.65782964315799 17.222010909788803 32.64703040302619 18-19 15.14119688655062 35.06918086405094 17.248475513460257 32.541146735938185 20-21 32.607167149950655 35.10376803053645 17.323490706932255 14.965574112580635 22-23 32.47307314997103 17.630362250080637 34.85037694511845 15.04618765482989 24-25 14.977486243080051 34.82105966426638 17.856120738620398 32.34533335403318 26-27 15.212291454748526 24.955444659646773 44.579414771607006 15.2528491139977 28-29 30.298223554065345 17.422630873644454 37.15897454456062 15.120171027729585 30-31 26.777121442059105 19.479470936198418 37.75941022831199 15.98399739343048 32-33 19.349886669143494 22.269727438487095 39.91038992881088 18.469995963558535 34-35 18.677748254453448 21.175195575145295 40.753192568944826 19.39386360145643 36-37 18.963601561795276 20.923040602448047 40.84922818972488 19.264129646031794 38-39 19.145061028638853 20.923158708188108 40.68713865502243 19.244641608150605 40-41 19.146360775334053 20.964594571530963 40.236163960969265 19.652880692165724 42-43 19.56732035521027 21.07314701996398 40.275733365311105 19.083799259514635 44-45 18.942197659409175 21.205844110792192 40.13467969126746 19.717278538531183 46-47 19.538321912966307 21.006289254305184 39.933758891164864 19.521629941563646 48-49 19.255424134390715 21.3030074971415 40.091039394977976 19.350528973489816 50-51 19.453345848627013 20.973881221668403 39.806439451592006 19.766333478112575 52-53 19.95561293056023 22.086874102573763 38.25538115357601 19.702131813289995 54-55 20.592685760505585 23.03168230038701 36.337930027379024 20.037701911728377 56-57 20.985718843373824 23.938926014483556 34.97041625643647 20.10493888570615 58-59 21.065433637693992 24.841278463765587 33.98783997372404 20.105447924816378 60-61 20.86723308615518 25.109623484509353 33.6330064962442 20.390136933091263 62-63 20.74413344945401 25.127256649025252 33.57575936222952 20.552850539291217 64-65 20.504786420332778 24.666943254819422 33.92599339264811 20.90227693219969 66-67 20.53887581588363 24.0708491493113 34.866048059990675 20.52422697481439 68-69 20.368787783952904 24.019682419399505 35.15584568885413 20.45568410779347 70-71 20.38048195910866 23.96807257145929 35.506839834524776 20.144605634907272 72-73 19.996638489000805 23.772475013232732 35.535153408600024 20.69573308916644 74-75 20.586390097066886 23.893694176463836 34.93665040479241 20.583265321676866 76-77 21.003251414721337 24.467592632299386 34.01993667846244 20.50921927451683 78-79 20.694801334071744 24.60555512964096 33.79881807805531 20.900825458231985 80-81 20.609872697849678 25.31104532543219 33.30394098672944 20.775140989988696 82-83 21.646127081381564 25.29685480119282 32.451351357145455 20.605666760280155 84-85 21.362683448268136 25.90058615222013 32.068828063784196 20.667902335727543 86-87 21.080509769138693 26.107792949274145 32.00363339793137 20.808063883655798 88-89 20.88257196555657 25.672082474032305 31.979274297034678 21.46607126337645 90-91 20.88334084310327 25.875558234272443 31.91723515249872 21.323865770125565 92-93 21.235769775631773 26.002179219229625 32.002644999997855 20.759406005140743 94-95 21.020623594495326 25.850621534527203 31.32348287529515 21.805271995682325 96-97 20.997691134959815 25.60007596488758 31.787005350248638 21.615227549903967 98-99 21.385302401520413 26.0373077123695 31.166478886362096 21.41091099974799 100-101 21.786349609797643 25.608864530466157 31.062622612455847 21.542163247280357 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 25030.0 1 14481.0 2 2693.5 3 1176.0 4 838.5 5 749.0 6 868.5 7 1070.5 8 982.0 9 1116.0 10 2281.5 11 14798.0 12 294001.0 13 318657.0 14 66175.0 15 57785.0 16 61317.5 17 65882.5 18 70059.0 19 72163.0 20 72608.0 21 72258.0 22 71850.5 23 72392.0 24 73899.0 25 76554.0 26 80716.0 27 86800.0 28 91741.0 29 95787.0 30 101340.0 31 108943.5 32 116573.0 33 124755.0 34 140898.5 35 151033.5 36 156285.5 37 166515.5 38 175026.5 39 184718.0 40 196795.0 41 209570.5 42 220486.5 43 230541.5 44 242607.5 45 253146.5 46 259371.5 47 277082.0 48 288702.0 49 288546.0 50 291910.5 51 292005.5 52 294683.0 53 301356.0 54 291598.0 55 271475.0 56 261906.0 57 252240.0 58 259263.5 59 229725.5 60 169728.5 61 138647.5 62 112046.5 63 87543.0 64 68744.0 65 56213.5 66 44903.0 67 35620.5 68 27766.5 69 21204.0 70 16773.5 71 14096.5 72 12021.0 73 9375.0 74 7202.0 75 5476.0 76 4124.5 77 3095.5 78 2346.0 79 1834.5 80 1459.5 81 1251.0 82 1083.5 83 898.5 84 735.0 85 641.0 86 512.0 87 363.5 88 240.0 89 160.0 90 121.0 91 106.0 92 78.0 93 56.5 94 46.5 95 34.5 96 25.5 97 14.5 98 13.0 99 9.5 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05195970975796497 2 0.09299856562078791 3 0.08406916155358354 4 0.05569635486522435 5 0.08575011651587501 6 0.001456113852685604 7 0.00124197946258478 8 0.0050963984843996136 9 0.009411206444931219 10-11 0.009518273639981631 12-13 0.01689520337895502 14-15 0.011499016748414255 16-17 0.0016220680050137426 18-19 6.745233288175959E-4 20-21 1.0706719505041206E-4 22-23 4.4432885945921E-4 24-25 8.993644384234613E-4 26-27 0.0014935873709532481 28-29 0.0016809549622914693 30-31 0.004823377137021063 32-33 2.034276705957829E-4 34-35 0.0024893122849220803 36-37 3.9079526193400393E-4 38-39 0.0027462735530430693 40-41 0.003383323363593021 42-43 0.0011670324260494914 44-45 6.959367678276784E-4 46-47 0.001172385785802012 48-49 0.0025267858031897245 50-51 0.00452358899087991 52-53 0.13075581195531571 54-55 0.140054597845444 56-57 0.12669261190315256 58-59 0.120434534352456 60-61 0.10871067649443587 62-63 0.1129987176562049 64-65 0.1153702560265715 66-67 0.12361978340520575 68-69 0.1362108855431342 70-71 0.12337888221634233 72-73 0.11553621017889966 74-75 0.12054160154750641 76-77 0.1316605297534917 78-79 0.14554714495153012 80-81 0.1356648428483771 82-83 0.11551479673988956 84-85 0.1218371146076164 86-87 0.12281677944232766 88-89 0.11912296121308844 90-91 0.12933717162089775 92-93 0.1461788414023276 94-95 0.1556971150423092 96-97 0.15597548974944028 98-99 0.13901069269370248 100-101 0.12861446805430748 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 9339929.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.11375901408247 #Duplication Level Percentage of deduplicated Percentage of total 1 89.32731667744791 39.405637212835664 2 6.000175236249452 5.293805688283473 3 1.7000345759537718 2.2498474679769767 4 0.8474048783249319 1.4952885835913567 5 0.512696893582531 1.1308493605384229 6 0.31057939482053043 0.8220494746711472 7 0.21777673924847754 0.6724865415856008 8 0.1759471964007214 0.6209353776979888 9 0.12772316709498524 0.507091411236921 >10 0.7542437451807726 5.47608644848975 >50 0.008744736697199393 0.2737577868349533 >100 0.009894003562513208 1.036661873748136 >500 0.003505992487058116 1.0630396045366284 >1k 0.0032555644518589707 2.8765900746089743 >5k 4.5077046256508825E-4 1.4737127515585864 >10k+ 2.504280347583824E-4 35.60216034180547 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 1524101 16.318121904352807 No Hit AAGCAGTGGTATCAACGCAGAGTATTTTTTTTTTTTTTTTTTTTTTTTTT 30975 0.33164063666865135 No Hit AAGCAGTGGTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAG 23625 0.2529462483065985 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 21829 0.23371698007554448 No Hit AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGAT 10818 0.11582529160553577 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 2.141343901008241E-5 0.0 0.0 0.0 0.0 2 8.565375604032964E-5 0.0 0.0 0.0 0.0 3 1.0706719505041206E-4 0.0 0.0 0.0 0.0 4 1.0706719505041206E-4 0.0 0.0 0.0 0.0 5 1.0706719505041206E-4 0.0 0.0 0.0 0.0 6 1.1777391455545326E-4 0.0 0.0 0.0 0.0 7 1.1777391455545326E-4 0.0 0.0 0.0 0.0 8 1.1777391455545326E-4 0.0 0.0 0.0 0.0 9 1.3918735356553566E-4 0.0 0.0 1.0706719505041205E-5 0.0 10-11 1.4989407307057688E-4 0.0 0.0 1.0706719505041205E-5 5.3533597525206025E-6 12-13 1.7130751208065928E-4 5.3533597525206025E-6 0.0 1.606007925756181E-5 1.606007925756181E-5 14-15 1.873675913382211E-4 1.0706719505041205E-5 0.0 2.141343901008241E-5 2.141343901008241E-5 16-17 2.1413439010082411E-4 1.0706719505041205E-5 0.0 2.141343901008241E-5 2.6766798762603014E-5 18-19 2.301944693583859E-4 1.0706719505041205E-5 0.0 2.141343901008241E-5 3.212015851512362E-5 20-21 2.8908142663611257E-4 1.0706719505041205E-5 0.0 2.141343901008241E-5 3.212015851512362E-5 22-23 2.9443478638863313E-4 1.0706719505041205E-5 0.0 2.141343901008241E-5 4.282687802016482E-5 24-25 3.3190830465627737E-4 1.0706719505041205E-5 0.0 2.141343901008241E-5 4.282687802016482E-5 26-27 3.64028463171401E-4 1.0706719505041205E-5 0.0 3.212015851512362E-5 4.282687802016482E-5 28-29 4.0150198143904523E-4 1.0706719505041205E-5 0.0 3.747351826764422E-5 4.282687802016482E-5 30-31 4.550355789642512E-4 1.0706719505041205E-5 5.3533597525206025E-6 4.282687802016482E-5 5.353359752520603E-5 32-33 4.978624569844161E-4 1.0706719505041205E-5 1.0706719505041205E-5 4.282687802016482E-5 5.353359752520603E-5 34-35 5.888695727772663E-4 1.0706719505041205E-5 1.0706719505041205E-5 5.353359752520603E-5 6.424031703024724E-5 36-37 6.905834080751578E-4 1.0706719505041205E-5 1.0706719505041205E-5 5.353359752520603E-5 6.424031703024724E-5 38-39 7.494703653528844E-4 1.0706719505041205E-5 1.0706719505041205E-5 8.565375604032964E-5 6.424031703024724E-5 40-41 7.976506031255698E-4 2.141343901008241E-5 1.0706719505041205E-5 8.565375604032964E-5 6.424031703024724E-5 42-43 8.190640421356522E-4 2.141343901008241E-5 1.0706719505041205E-5 9.636047554537084E-5 6.424031703024724E-5 44-45 8.779509994133788E-4 2.141343901008241E-5 1.0706719505041205E-5 9.636047554537084E-5 6.424031703024724E-5 46-47 9.743114749587497E-4 2.6766798762603014E-5 1.0706719505041205E-5 1.0171383529789145E-4 8.565375604032964E-5 48-49 0.0010546118712465588 3.212015851512362E-5 1.0706719505041205E-5 1.0706719505041206E-4 8.565375604032964E-5 50-51 0.0011134988285242853 3.212015851512362E-5 1.0706719505041205E-5 1.0706719505041206E-4 8.565375604032964E-5 52-53 0.0011509723467919295 3.747351826764422E-5 1.0706719505041205E-5 1.0706719505041206E-4 8.565375604032964E-5 54-55 0.0011830925053070533 4.282687802016482E-5 1.0706719505041205E-5 1.0706719505041206E-4 8.565375604032964E-5 56-57 0.0012901597003574653 4.282687802016482E-5 1.0706719505041205E-5 1.0706719505041206E-4 9.636047554537084E-5 58-59 0.0013436932978826712 4.282687802016482E-5 1.0706719505041205E-5 1.0706719505041206E-4 9.636047554537084E-5 60-61 0.0013865201759028362 4.282687802016482E-5 1.0706719505041205E-5 1.0706719505041206E-4 9.636047554537084E-5 62-63 0.0014614672124381245 4.282687802016482E-5 1.0706719505041205E-5 1.0706719505041206E-4 1.0706719505041204E-4 64-65 0.0015578276879834954 4.282687802016482E-5 1.0706719505041205E-5 1.0706719505041206E-4 1.1777391455545326E-4 66-67 0.0016381280842713043 4.282687802016482E-5 1.0706719505041205E-5 1.0706719505041206E-4 1.2312727430797385E-4 68-69 0.0017826687975893607 4.282687802016482E-5 1.0706719505041205E-5 1.0706719505041206E-4 1.3918735356553566E-4 70-71 0.0018522624743721285 4.282687802016482E-5 1.0706719505041205E-5 1.0706719505041206E-4 1.4454071331805628E-4 72-73 0.0019593296694225405 4.282687802016482E-5 1.0706719505041205E-5 1.1242055480293266E-4 1.4989407307057688E-4 74-75 0.002125283821750679 4.282687802016482E-5 1.0706719505041205E-5 1.1777391455545326E-4 1.606007925756181E-4 76-77 0.002334064852098983 5.353359752520603E-5 1.0706719505041205E-5 1.1777391455545326E-4 1.873675913382211E-4 78-79 0.002532139162942245 5.353359752520603E-5 1.0706719505041205E-5 1.1777391455545326E-4 1.9272095109074168E-4 80-81 0.002901520985866167 6.424031703024724E-5 1.0706719505041205E-5 1.2848063406049447E-4 1.9272095109074168E-4 82-83 0.003420796881860665 6.424031703024724E-5 1.0706719505041205E-5 1.3918735356553566E-4 2.034276705957829E-4 84-85 0.004063200052163138 6.424031703024724E-5 1.0706719505041205E-5 1.3918735356553566E-4 2.1413439010082411E-4 86-87 0.004807317057763501 6.424031703024724E-5 1.0706719505041205E-5 1.3918735356553566E-4 2.1413439010082411E-4 88-89 0.005985056203318033 6.424031703024724E-5 1.0706719505041205E-5 1.3918735356553566E-4 2.1413439010082411E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AAGCAGT 328580 0.0 92.37643 1 AGTGGTA 328200 0.0 92.04191 5 TGGTATC 330560 0.0 91.57609 7 GGTATCA 330495 0.0 91.575424 8 AGCAGTG 331590 0.0 91.51405 2 GTGGTAT 331035 0.0 91.48291 6 GTATCAA 330715 0.0 91.48103 9 GCAGTGG 332005 0.0 91.19643 3 CAGTGGT 332575 0.0 90.91685 4 ATCAACG 330660 0.0 46.174988 10-11 CAACGCA 331170 0.0 46.154522 12-13 TCAACGC 331095 0.0 46.116943 12-13 ACGCAGA 332160 0.0 46.04034 14-15 AACGCAG 332040 0.0 45.99979 14-15 CGCAGAG 332845 0.0 45.890907 16-17 AGAGTAC 325710 0.0 45.84922 18-19 TATCAAC 332605 0.0 45.834316 10-11 CAGAGTA 330585 0.0 45.75597 18-19 GAGTACT 180970 0.0 45.59762 20-21 GCAGAGT 334270 0.0 45.555416 16-17 >>END_MODULE