FastQCFastQC Report
Sat 18 Jun 2016
SRR578602_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR578602_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9339929
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2372492.540158495851521No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT211840.22681114599479288No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT211340.22627581001954086No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT107700.027.3896311
AGTGGTA120150.023.7175145
GGTATCA126600.022.0971768
GTATCAA128650.021.6698469
TGGTATC137800.020.4732257
ATCAACG113250.020.2813710-11
GTGGTAT141000.020.210356
AGCAGTG146800.019.9964472
CAACGCA115100.019.7906412-13
GCAGTGG154200.018.8504853
ACGCAGA129700.017.61768514-15
CAGTGGT171500.017.0033364
AGAGTAC133650.016.69024318-19
TCAACGC111500.015.51885710-11
AGTACTT108550.015.28763820-21
AACGCAG119350.014.47827612-13
CGTCACG5652.0008883E-1114.2674673
GCAGAGT165350.013.90530316-17
CGCAGAG131500.013.2126514-15
CGCGTAG2752.8358045E-912.9291654-55
Produced by FastQC (version 0.11.5)