##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR578602_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 9339929 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.991874884702014 31.0 31.0 34.0 30.0 34.0 2 32.263192257671335 34.0 31.0 34.0 30.0 34.0 3 32.33578916927527 34.0 31.0 34.0 30.0 34.0 4 35.901571521582234 37.0 35.0 37.0 35.0 37.0 5 35.780726384536756 37.0 35.0 37.0 33.0 37.0 6 35.75286418130159 37.0 35.0 37.0 33.0 37.0 7 35.741128224850534 37.0 35.0 37.0 33.0 37.0 8 35.66041926014641 37.0 35.0 37.0 33.0 37.0 9 37.295907923925334 39.0 37.0 39.0 33.0 39.0 10-11 37.28856177600494 39.0 37.0 39.0 33.0 39.0 12-13 37.28426543713555 39.0 37.0 39.0 33.0 39.0 14-15 38.59500650379677 40.0 38.0 41.0 34.0 41.0 16-17 38.53132689766699 40.0 38.0 41.0 34.0 41.0 18-19 38.41652409777419 40.0 38.0 41.0 33.5 41.0 20-21 38.32247065261417 40.0 38.0 41.0 33.5 41.0 22-23 38.18942461982313 40.0 37.0 41.0 33.0 41.0 24-25 38.09734865222209 40.0 37.0 41.0 33.0 41.0 26-27 37.92374974156655 40.0 37.0 41.0 33.0 41.0 28-29 37.81745434039166 40.0 37.0 41.0 33.0 41.0 30-31 37.71125192707568 40.0 37.0 41.0 32.5 41.0 32-33 37.5690326446807 40.0 36.5 41.0 32.0 41.0 34-35 37.422236293230924 40.0 36.0 41.0 31.5 41.0 36-37 37.27828771503509 39.5 36.0 41.0 31.0 41.0 38-39 37.12482509235349 39.0 35.5 41.0 31.0 41.0 40-41 36.96079798893546 39.0 35.0 41.0 31.0 41.0 42-43 36.78332892038044 39.0 35.0 41.0 30.0 41.0 44-45 36.61028145931302 39.0 35.0 40.5 30.0 41.0 46-47 36.67544009167521 39.0 35.0 41.0 30.0 41.0 48-49 36.635860079878555 39.0 35.0 41.0 30.0 41.0 50-51 36.58283333845472 39.0 35.0 41.0 30.0 41.0 52-53 36.56281086290913 39.0 35.0 41.0 30.0 41.0 54-55 36.408051388827474 39.0 35.0 41.0 30.0 41.0 56-57 36.24101291348146 39.0 35.0 41.0 30.0 41.0 58-59 36.014898186056875 38.0 35.0 40.5 29.0 41.0 60-61 35.77824135493964 38.0 35.0 40.0 29.0 41.0 62-63 35.49965529716553 37.5 34.0 40.0 28.5 41.0 64-65 35.186540068987675 37.0 34.0 40.0 28.0 41.0 66-67 34.87003423687696 36.0 34.0 39.5 28.0 41.0 68-69 34.544055474083365 36.0 34.0 39.0 28.0 41.0 70-71 34.18880470076378 35.5 33.5 39.0 27.5 41.0 72-73 33.778635790486206 35.0 33.0 38.0 26.5 40.0 74-75 33.412352599254234 35.0 33.0 37.0 26.0 39.5 76-77 32.00133732279978 34.0 30.5 36.0 24.5 39.0 78-79 32.61976643505534 35.0 32.0 36.0 26.0 39.0 80-81 32.5850422952894 35.0 33.0 36.0 26.0 38.5 82-83 32.32787460161635 35.0 32.5 35.5 26.0 37.0 84-85 32.05856709403251 35.0 32.0 35.0 25.5 37.0 86-87 31.79275099414567 35.0 32.0 35.0 25.0 36.5 88-89 31.551681495651625 35.0 32.0 35.0 24.5 36.0 90-91 31.316366484156354 35.0 32.0 35.0 24.0 36.0 92-93 31.076154165625884 35.0 32.0 35.0 23.5 36.0 94-95 30.81767902090048 34.5 31.5 35.0 21.5 35.0 96-97 30.214521973346905 34.0 31.0 35.0 13.0 35.0 98-99 30.16659286168021 34.0 31.0 35.0 15.5 35.0 100-101 29.5662616921392 34.0 30.5 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.4343741077206431 1101 2 0.3978996353643751 1101 3 0.39639013199561646 1101 4 0.27525025242582757 1101 5 0.2939423700084376 1101 6 0.28219804097965806 1101 7 0.3013683212966711 1101 8 0.36697430728772673 1101 9 0.44721922875092446 1101 10-11 0.42618997432901295 1101 12-13 0.42467783558146266 1101 14-15 0.5693940977418492 1101 16-17 0.5661891543366906 1101 18-19 0.5638417395049871 1101 20-21 0.5948753488399632 1101 22-23 0.6455843501454481 1101 24-25 0.6854121886247668 1101 26-27 0.6893196203118137 1101 28-29 0.6636894408438536 1101 30-31 0.6372210478091702 1101 32-33 0.6367894314734386 1101 34-35 0.6772897790237167 1101 36-37 0.6847878514449661 1101 38-39 0.7211994960511632 1101 40-41 0.725738201995334 1101 42-43 0.7560853230161158 1101 44-45 0.7268287199738168 1101 46-47 0.7235380154697211 1101 48-49 0.7149144785128669 1101 50-51 0.7033604391412922 1101 52-53 0.6898748595946742 1101 54-55 0.730295574214999 1101 56-57 0.7492035508505523 1101 58-59 0.7560820475679648 1101 60-61 0.6591285302558347 1101 62-63 0.6935043473831897 1101 64-65 0.675362553081321 1101 66-67 0.650568060704046 1101 68-69 0.6565976136619582 1101 70-71 0.6500192325445298 1101 72-73 0.6392931925248817 1101 74-75 0.6783947505819015 1101 76-77 0.6755825146202383 1101 78-79 0.5789596561094399 1101 80-81 0.5119883755791506 1101 82-83 0.5424090503795185 1101 84-85 0.49754492267206274 1101 86-87 0.44928653403357544 1101 88-89 0.4804949794114357 1101 90-91 0.5235508822843045 1101 92-93 0.5189545355771195 1101 94-95 0.5237813223449947 1101 96-97 0.9151190465565229 1101 98-99 0.7483655042586719 1101 100-101 0.7228003285777547 1102 1 0.41119834444142356 1102 2 0.38846690305311427 1102 3 0.3567979530630794 1102 4 0.2685546629074764 1102 5 0.29401153687879145 1102 6 0.30930211014631226 1102 7 0.2526910532078759 1102 8 0.29298145288984045 1102 9 0.40137872880688974 1102 10-11 0.36120229086717615 1102 12-13 0.40737042993343664 1102 14-15 0.5071521775643575 1102 16-17 0.5182795996857479 1102 18-19 0.5421107997071246 1102 20-21 0.5732118729903988 1102 22-23 0.5647043495567488 1102 24-25 0.6501809416038924 1102 26-27 0.7025931565519272 1102 28-29 0.6912502681166899 1102 30-31 0.7338574626390724 1102 32-33 0.7613099243580237 1102 34-35 0.7930363189640417 1102 36-37 0.7737803212295304 1102 38-39 0.7689411143526357 1102 40-41 0.7964930251073525 1102 42-43 0.8218994398446497 1102 44-45 0.8421918377491551 1102 46-47 0.8775958722553128 1102 48-49 0.8911509053638156 1102 50-51 0.85406638944918 1102 52-53 0.7657038345504859 1102 54-55 0.7731874866816995 1102 56-57 0.7112121539421778 1102 58-59 0.7575012433959287 1102 60-61 0.8126184316545348 1102 62-63 0.7621698924859786 1102 64-65 0.7310518409586777 1102 66-67 0.7580238672738417 1102 68-69 0.7944117999132345 1102 70-71 0.7625925625299033 1102 72-73 0.7607484301245862 1102 74-75 0.6875814018329791 1102 76-77 0.6725941555306889 1102 78-79 0.7043123087670082 1102 80-81 0.7289943181418295 1102 82-83 0.6890456875598687 1102 84-85 0.7085875895517688 1102 86-87 0.7233596666476316 1102 88-89 0.6539241436686503 1102 90-91 0.61663249585607 1102 92-93 0.6445814778016334 1102 94-95 0.6837328864771379 1102 96-97 1.1335111500240203 1102 98-99 0.882684482700256 1102 100-101 0.9096678595770697 1103 1 0.3430691769612366 1103 2 0.29520575077220457 1103 3 0.27490733760265584 1103 4 0.2597897385936463 1103 5 0.23190342454456925 1103 6 0.29926186627901785 1103 7 0.3141238158873847 1103 8 0.09583868469123047 1103 9 0.20032677625017214 1103 10-11 0.39965092264602475 1103 12-13 0.38524594197324546 1103 14-15 0.501929379355218 1103 16-17 0.4939703393020167 1103 18-19 0.3829478898535186 1103 20-21 0.4120601665659933 1103 22-23 0.4428297499487357 1103 24-25 0.30866694182464016 1103 26-27 0.27008289129128826 1103 28-29 0.4931597930450806 1103 30-31 0.3495971545999197 1103 32-33 0.5574626924310238 1103 34-35 0.5309694303475965 1103 36-37 0.5738415195752609 1103 38-39 0.6196443755278267 1103 40-41 0.498076587708546 1103 42-43 0.5964047825740977 1103 44-45 0.6737635384146543 1103 46-47 0.5069079526305487 1103 48-49 0.5787559211068682 1103 50-51 0.5454969062206629 1103 52-53 0.5862368736730943 1103 54-55 0.6843079964213032 1103 56-57 0.6334503228635455 1103 58-59 0.3428618230198026 1103 60-61 0.3403576349910793 1103 62-63 0.21535308891713356 1103 64-65 0.475729094776824 1103 66-67 0.4337994305580608 1103 68-69 0.41968838662947405 1103 70-71 0.40403600006505513 1103 72-73 0.5948971341033769 1103 74-75 0.5405576001008328 1103 76-77 0.3688667581318086 1103 78-79 0.4630397446760597 1103 80-81 0.5283437347232365 1103 82-83 0.49629076085242474 1103 84-85 0.5545326237965043 1103 86-87 0.41017223939456926 1103 88-89 0.42641210365943394 1103 90-91 0.45743472826193354 1103 92-93 0.45393887663581367 1103 94-95 0.4594562958350572 1103 96-97 0.6221628990168355 1103 98-99 0.4034895486251102 1103 100-101 0.619064166914228 1104 1 0.3228193701358819 1104 2 0.3074826275217646 1104 3 0.2794401270370841 1104 4 0.20057151040276722 1104 5 0.22390812147817485 1104 6 0.22338713647950215 1104 7 0.20908663823487927 1104 8 0.299234710805969 1104 9 0.30702523489679123 1104 10-11 0.32172226501718626 1104 12-13 0.31627208658412087 1104 14-15 0.36999104421476403 1104 16-17 0.4409686508922803 1104 18-19 0.4631419445693794 1104 20-21 0.39862985076808855 1104 22-23 0.5072021749787865 1104 24-25 0.4422161974740533 1104 26-27 0.5520046935455909 1104 28-29 0.5580959443163849 1104 30-31 0.5497881945453784 1104 32-33 0.5692348958443247 1104 34-35 0.5511922359718611 1104 36-37 0.6141464848067812 1104 38-39 0.6106775308714631 1104 40-41 0.5812011754279496 1104 42-43 0.6156252306055094 1104 44-45 0.5764657801883075 1104 46-47 0.45226260330302637 1104 48-49 0.5881877832995883 1104 50-51 0.4852276321160005 1104 52-53 0.5799277129985185 1104 54-55 0.607187424911622 1104 56-57 0.6212623457334487 1104 58-59 0.6421586275116837 1104 60-61 0.5518518787952758 1104 62-63 0.3830194065379331 1104 64-65 0.5137297553440519 1104 66-67 0.569535548259104 1104 68-69 0.6551633757629531 1104 70-71 0.6016773750039661 1104 72-73 0.6910462968892901 1104 74-75 0.6789909385481536 1104 76-77 0.5772317704748176 1104 78-79 0.5423903257429217 1104 80-81 0.6931518985441869 1104 82-83 0.7446032042064417 1104 84-85 0.7776848621969279 1104 86-87 0.7092676387900809 1104 88-89 0.6536076493449521 1104 90-91 0.6917675612043759 1104 92-93 0.7450630748033085 1104 94-95 0.8368262839606508 1104 96-97 1.1807620863404864 1104 98-99 0.8990428246334758 1104 100-101 0.9437221506612197 1105 1 0.17381694780584667 1105 2 0.14126355587635686 1105 3 0.12414924788418347 1105 4 0.07057678126888334 1105 5 0.11166047183554184 1105 6 0.06931982958919747 1105 7 0.1116692429868067 1105 8 0.1688441955000073 1105 9 0.19458351104248095 1105 10-11 0.16627747949099358 1105 12-13 0.1666964356418248 1105 14-15 0.1859670895795773 1105 16-17 0.178834587357386 1105 18-19 0.10621511372685433 1105 20-21 0.17962103649465178 1105 22-23 0.16345271021533136 1105 24-25 0.19357998224930384 1105 26-27 0.164296145513255 1105 28-29 0.23020782183023414 1105 30-31 0.18722555742545666 1105 32-33 0.22665136784264206 1105 34-35 0.198595377640153 1105 36-37 0.19003970435928608 1105 38-39 0.22624404047749636 1105 40-41 0.2496584393473924 1105 42-43 0.16646396686753206 1105 44-45 0.29734217563245835 1105 46-47 0.2765748621372168 1105 48-49 0.29677912625888325 1105 50-51 0.19954439787753842 1105 52-53 0.245399711200605 1105 54-55 0.35435344726207063 1105 56-57 0.3259918683841434 1105 58-59 0.3066690356079107 1105 60-61 0.30591335821441845 1105 62-63 0.33569171140382537 1105 64-65 0.30131108896807035 1105 66-67 0.297058776480462 1105 68-69 0.2807291694529681 1105 70-71 0.32731530854187696 1105 72-73 0.3566090507225326 1105 74-75 0.3614830041810677 1105 76-77 0.37456243629982566 1105 78-79 0.36320831863336167 1105 80-81 0.26109794630669825 1105 82-83 0.2803859094685208 1105 84-85 0.32895873296848777 1105 86-87 0.2713616795382663 1105 88-89 0.23672189320053505 1105 90-91 0.26900826156101587 1105 92-93 0.28012285731464104 1105 94-95 0.3850752743395951 1105 96-97 0.7345474939232908 1105 98-99 0.5117580733152138 1105 100-101 0.492093570382508 1106 1 -0.0666283143450137 1106 2 -0.123933230387415 1106 3 -0.07756777360420841 1106 4 -0.0490942942116277 1106 5 -0.04876363335178269 1106 6 -0.05841511471166427 1106 7 -0.07657289501933207 1106 8 -0.05252856916211357 1106 9 0.0022622617225138697 1106 10-11 -0.08638619699581795 1106 12-13 -0.07511318375898668 1106 14-15 -0.15876194557175438 1106 16-17 -0.13463309536087564 1106 18-19 -0.13516628138547304 1106 20-21 -0.16005869560254382 1106 22-23 -0.11715364868850742 1106 24-25 -0.05131095732679114 1106 26-27 -0.10523399298600822 1106 28-29 -0.11082214883602859 1106 30-31 -0.24735360028402198 1106 32-33 -0.3007940357192993 1106 34-35 -0.3302467722804536 1106 36-37 -0.23656598844584664 1106 38-39 -0.29182917562979327 1106 40-41 -0.3162191860671726 1106 42-43 -0.22467076999301128 1106 44-45 -0.20512404927686845 1106 46-47 -0.24654351898350768 1106 48-49 -0.21827171152116875 1106 50-51 -0.21252905295546753 1106 52-53 -0.10207888565296486 1106 54-55 -0.12725760325289315 1106 56-57 -0.16516600960615335 1106 58-59 -0.12699192064980735 1106 60-61 -0.2366517981396612 1106 62-63 -0.19407306210236897 1106 64-65 -0.13903443780431957 1106 66-67 -0.14305032873794943 1106 68-69 -0.11580321349414646 1106 70-71 -0.1278195582538899 1106 72-73 -0.11650396191027568 1106 74-75 -0.12296822626801429 1106 76-77 -0.21921198550742105 1106 78-79 -0.11821374017384301 1106 80-81 -0.18190829453402557 1106 82-83 -0.13673256557738256 1106 84-85 -0.1492659075175844 1106 86-87 -0.20537509919429908 1106 88-89 -0.24748170398416747 1106 90-91 -0.21819115498147212 1106 92-93 -0.20443271630408688 1106 94-95 -0.14735584899466758 1106 96-97 0.1606544014321507 1106 98-99 -0.03201444020858446 1106 100-101 -0.020095785462864058 1107 1 -0.18115085965825628 1107 2 -0.2639727284380413 1107 3 -0.19144562124836995 1107 4 -0.15524214088573274 1107 5 -0.13934702883837247 1107 6 -0.1505212961464153 1107 7 -0.15005493279943494 1107 8 -0.15585905677866663 1107 9 -0.18935099017602397 1107 10-11 -0.23819841864132485 1107 12-13 -0.20028266689889307 1107 14-15 -0.24459349237398698 1107 16-17 -0.25519147946922516 1107 18-19 -0.25096347967631516 1107 20-21 -0.25299072011874557 1107 22-23 -0.19271200702230118 1107 24-25 -0.1545995164263232 1107 26-27 -0.15690121646310473 1107 28-29 -0.16598252309904638 1107 30-31 -0.24580626049885979 1107 32-33 -0.332952427604603 1107 34-35 -0.31051725219160886 1107 36-37 -0.3158974389678306 1107 38-39 -0.33718544692401764 1107 40-41 -0.3496058744471 1107 42-43 -0.35221837602762207 1107 44-45 -0.24884945750670084 1107 46-47 -0.27260037724382613 1107 48-49 -0.4202575079459905 1107 50-51 -0.39156064880136654 1107 52-53 -0.4130408777146144 1107 54-55 -0.29955868514075945 1107 56-57 -0.33378443428102855 1107 58-59 -0.3132819219472296 1107 60-61 -0.3669604295380253 1107 62-63 -0.4973167723304712 1107 64-65 -0.4114048412479079 1107 66-67 -0.33898613264997124 1107 68-69 -0.337877923338084 1107 70-71 -0.37904029211643575 1107 72-73 -0.33258338416610655 1107 74-75 -0.3341392551269351 1107 76-77 -0.3827987252512983 1107 78-79 -0.24334559131815325 1107 80-81 -0.2585999481188139 1107 82-83 -0.24818554751126953 1107 84-85 -0.2576057836449479 1107 86-87 -0.2931374700356244 1107 88-89 -0.287979926277 1107 90-91 -0.24477522350974468 1107 92-93 -0.2411746827377037 1107 94-95 -0.21868401052320863 1107 96-97 0.0797886321338126 1107 98-99 -0.1893736196043072 1107 100-101 -0.2557995436138114 1108 1 -0.3444644081593502 1108 2 -0.36197270058084285 1108 3 -0.3417970739451306 1108 4 -0.20495611073426545 1108 5 -0.23948458024901242 1108 6 -0.2394575324620618 1108 7 -0.2749531932171223 1108 8 -0.2618330663769939 1108 9 -0.3530210293171905 1108 10-11 -0.3824983866010214 1108 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2305 24-25 -0.03270071841686928 2305 26-27 -0.1006360041350689 2305 28-29 -0.12067068607565545 2305 30-31 -0.13978900956843887 2305 32-33 -0.16698507604939294 2305 34-35 -0.10720821227615573 2305 36-37 -0.03506453279558741 2305 38-39 -0.09638325045919771 2305 40-41 -0.09793264660827816 2305 42-43 -0.15026017933453772 2305 44-45 -0.18387458514343535 2305 46-47 -0.08132498930364562 2305 48-49 -0.10686340890262613 2305 50-51 -0.13633498241204478 2305 52-53 -0.19733499144864908 2305 54-55 -0.09293747265655128 2305 56-57 -0.1379567132427013 2305 58-59 -0.0590596092646436 2305 60-61 -0.09810320843040898 2305 62-63 -0.12757026103453484 2305 64-65 -0.06549911443879353 2305 66-67 -0.13170814439263978 2305 68-69 -0.06873613960210179 2305 70-71 -0.07079104754409826 2305 72-73 -0.0943209764882127 2305 74-75 -0.13148832196365134 2305 76-77 -0.1618834250980541 2305 78-79 -0.13417620418575638 2305 80-81 -0.12513263718619783 2305 82-83 -0.08228298413434487 2305 84-85 -0.06819786676525297 2305 86-87 -0.02504610605993207 2305 88-89 -0.010937811354612137 2305 90-91 -0.025219241172056428 2305 92-93 -0.0331738238125574 2305 94-95 -0.1894504108609638 2305 96-97 0.1219966675981965 2305 98-99 -0.13928455214978896 2305 100-101 -0.05949375153680592 2306 1 -0.2598665056804066 2306 2 -0.26474102813175904 2306 3 -0.24880205322948257 2306 4 -0.22751704405782647 2306 5 -0.19605572972463392 2306 6 -0.15307200151342215 2306 7 -0.2046670181119623 2306 8 -0.1913048619905453 2306 9 -0.38683201465277506 2306 10-11 -0.29018279151412685 2306 12-13 -0.30581852324144165 2306 14-15 -0.403760839357858 2306 16-17 -0.4104241616491322 2306 18-19 -0.3821372103263272 2306 20-21 -0.42647335628579697 2306 22-23 -0.4113774642704371 2306 24-25 -0.4050212366722121 2306 26-27 -0.4442630462638135 2306 28-29 -0.5389680519555213 2306 30-31 -0.555934202175159 2306 32-33 -0.6247032036188287 2306 34-35 -0.6099194821535292 2306 36-37 -0.6034434505747228 2306 38-39 -0.7834773165690265 2306 40-41 -0.6126617785931288 2306 42-43 -0.7435872056689661 2306 44-45 -0.7185141971166686 2306 46-47 -0.6149317927996805 2306 48-49 -0.5873015067745442 2306 50-51 -0.6178313249135314 2306 52-53 -0.5765643705114272 2306 54-55 -0.612121860125022 2306 56-57 -0.6294261400979693 2306 58-59 -0.5770321800396303 2306 60-61 -0.5745764331111829 2306 62-63 -0.5250118969689126 2306 64-65 -0.5933585332004085 2306 66-67 -0.5984216453866438 2306 68-69 -0.6242444913175973 2306 70-71 -0.6994813128622681 2306 72-73 -0.6624650686119935 2306 74-75 -0.6620688820505976 2306 76-77 -0.6538738131496622 2306 78-79 -0.5873255169786589 2306 80-81 -0.6045062184361001 2306 82-83 -0.602243254238239 2306 84-85 -0.5539409692297923 2306 86-87 -0.5848281454299382 2306 88-89 -0.564308208822581 2306 90-91 -0.5676881488399736 2306 92-93 -0.5924949681943694 2306 94-95 -0.6249868866987107 2306 96-97 -0.34977855714145534 2306 98-99 -0.5991899959117468 2306 100-101 -0.5898627059854107 2307 1 -0.4332110115793064 2307 2 -0.4321267122871859 2307 3 -0.44962482060266495 2307 4 -0.34681717814878965 2307 5 -0.3425352545668474 2307 6 -0.3311178525233487 2307 7 -0.3775789730922199 2307 8 -0.34720430054093043 2307 9 -0.4251653872614227 2307 10-11 -0.5762269808539955 2307 12-13 -0.4975471998629928 2307 14-15 -0.8327322365522676 2307 16-17 -0.6982017390267217 2307 18-19 -0.6575426901045418 2307 20-21 -0.6040693653302185 2307 22-23 -0.5776837303908806 2307 24-25 -0.5792661741743785 2307 26-27 -0.6570055920435109 2307 28-29 -0.6822247941035755 2307 30-31 -0.6593713884455781 2307 32-33 -0.6992947124329731 2307 34-35 -0.7078385994736465 2307 36-37 -0.8122473129739163 2307 38-39 -0.7748760589374584 2307 40-41 -0.67691910115105 2307 42-43 -0.7767618919582944 2307 44-45 -0.7037812478094239 2307 46-47 -0.7333145375401955 2307 48-49 -0.6473115329693755 2307 50-51 -0.7344398884380396 2307 52-53 -0.8537646060047663 2307 54-55 -0.8783550948809236 2307 56-57 -0.8476650858447741 2307 58-59 -0.8451736320270768 2307 60-61 -0.905047645531468 2307 62-63 -0.8758074085356284 2307 64-65 -0.8721608771943536 2307 66-67 -0.8130306153205566 2307 68-69 -0.7804506601577685 2307 70-71 -0.8519127907721398 2307 72-73 -1.1970322764564472 2307 74-75 -0.9385562931235469 2307 76-77 -0.846675066154674 2307 78-79 -0.8655841906433892 2307 80-81 -0.8008589822038772 2307 82-83 -0.7897316968466654 2307 84-85 -0.8258902439372626 2307 86-87 -0.7845331968334008 2307 88-89 -0.8587156798231668 2307 90-91 -0.9388472618909738 2307 92-93 -0.8153603116649109 2307 94-95 -0.8422678796233143 2307 96-97 -0.7055321896935851 2307 98-99 -1.0229359567700413 2307 100-101 -1.0275255273826858 2308 1 -1.3737363705309598 2308 2 -0.869401949010367 2308 3 -0.8969243973042396 2308 4 -0.5632539823610827 2308 5 -0.6473310830394681 2308 6 -0.66160899558718 2308 7 -0.6370815528462828 2308 8 -0.5981270812492525 2308 9 -0.8185649933315986 2308 10-11 -0.801587583804789 2308 12-13 -0.7929774251235457 2308 14-15 -1.009631252097769 2308 16-17 -0.9385717471970807 2308 18-19 -0.9749236408687807 2308 20-21 -0.967793811487546 2308 22-23 -1.0758588663493356 2308 24-25 -1.221848829531993 2308 26-27 -1.2547274553750114 2308 28-29 -1.2766380391590104 2308 30-31 -1.183535638431266 2308 32-33 -1.2170054647264053 2308 34-35 -1.2670687285699387 2308 36-37 -1.2924540073019273 2308 38-39 -1.3235157549180627 2308 40-41 -1.2856076025638217 2308 42-43 -1.1849026255224047 2308 44-45 -1.1684301030706692 2308 46-47 -1.1750237290887569 2308 48-49 -1.25909474610053 2308 50-51 -1.2931377014802976 2308 52-53 -1.2044115142240273 2308 54-55 -1.169619391552068 2308 56-57 -1.1007289732380485 2308 58-59 -1.0746464806277274 2308 60-61 -1.0199736756389868 2308 62-63 -1.0449897633972185 2308 64-65 -1.0248423134719715 2308 66-67 -0.9981244935393079 2308 68-69 -1.110919102910934 2308 70-71 -1.0831865815824315 2308 72-73 -1.122704764543066 2308 74-75 -1.2106277931719305 2308 76-77 -1.1938973542432372 2308 78-79 -1.5470797221390455 2308 80-81 -1.239932032111227 2308 82-83 -1.1634473132915808 2308 84-85 -1.0929873479690713 2308 86-87 -1.1214803131908972 2308 88-89 -1.1604309754301418 2308 90-91 -1.2005448782683104 2308 92-93 -1.1295098829172439 2308 94-95 -1.2807207672375576 2308 96-97 -1.109741703014194 2308 98-99 -1.4604796429952955 2308 100-101 -1.4837552607959168 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 63.0 3 123.0 4 345.0 5 879.0 6 1607.0 7 2880.0 8 4616.0 9 7089.0 10 9049.0 11 11417.0 12 13426.0 13 15273.0 14 16835.0 15 18629.0 16 21443.0 17 24857.0 18 29577.0 19 34250.0 20 36791.0 21 37161.0 22 40068.0 23 47082.0 24 58248.0 25 72191.0 26 90839.0 27 115757.0 28 145019.0 29 181920.0 30 223991.0 31 275953.0 32 340034.0 33 429411.0 34 575270.0 35 913087.0 36 1153301.0 37 1832101.0 38 2016045.0 39 541551.0 40 1751.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 17.684850102090685 30.40400670067833 28.336375851344652 23.574767345886325 2 23.101408796698912 29.975339070380723 26.22833200171381 20.694920131206555 3 22.60396439804591 28.413946329385 26.507169912333534 22.474919360235564 4 22.22583159874478 28.153054790585436 27.043114407040527 22.577999203629258 5 22.725177112090048 28.43016288916051 27.5608446732752 21.283815325474247 6 22.843492707492743 28.41064423509001 26.281902142939202 22.463960914478044 7 23.012637462233386 28.403395785985097 27.17441428087944 21.409552470902078 8 22.315164673069475 28.753456386991992 26.767222401156754 22.164156538781775 9 21.81925824336735 28.455509068268924 27.50976470375339 22.21546798461033 10-11 21.8290524478291 28.977677453436744 26.780390943014666 22.41287915571949 12-13 21.837141374415157 29.108294078038494 26.58265389383581 22.471910653710538 14-15 22.06577266272581 29.176078319224914 26.404965176930144 22.353183841119133 16-17 22.31046938365377 28.8287041582436 26.449676437583197 22.411150020519425 18-19 22.164932945421747 29.023951895137536 26.444901240683947 22.36621391875677 20-21 22.300217699727696 28.862606985556315 26.570517827276845 22.266657487439147 22-23 22.20107583374117 28.78746592984372 26.67647867181718 22.334979564597937 24-25 22.166556551785465 28.772068191957196 26.598533741970986 22.462841514286357 26-27 22.373404606558445 28.602600320592458 26.828045420887488 22.195949651961612 28-29 22.386321137987235 28.66990744790458 26.71991939125019 22.22385202285799 30-31 22.28367581809241 28.7036068475467 26.708291893867713 22.304425440493176 32-33 22.314398749712122 28.765320378773758 26.629945473889578 22.290335397624542 34-35 22.299302275210014 28.790106434427926 26.62335548803422 22.287235802327835 36-37 22.325132236015925 28.786417969558443 26.5868830480403 22.301566746385333 38-39 22.33003591354924 28.756230373913976 26.577632442387944 22.33610127014884 40-41 22.31558184221743 28.82757995269557 26.556047695865782 22.300790509221216 42-43 22.325640805192418 28.811996322455986 26.528916868640007 22.33344600371159 44-45 22.37553411808591 28.825727690221196 26.501320299115765 22.29741789257713 46-47 22.333559619862186 28.879589388719623 26.479164160624997 22.30768683079319 48-49 22.370089751217595 28.8704335975145 26.460773952350173 22.298702698917733 50-51 22.38853742892478 28.86008555311288 26.457642236894944 22.293734781067393 52-53 22.364864872099137 28.930284159547682 26.389568914281895 22.31528205407129 54-55 22.387043841553826 28.935123596763958 26.29623844035645 22.38159412132576 56-57 22.40136407889182 28.99495809871788 26.300130332896536 22.303547489493763 58-59 22.397611373705303 29.049482067797307 26.244953253927306 22.307953304570088 60-61 22.364007205625313 29.073991493507712 26.228599266054065 22.333402034812913 62-63 22.364702647030594 29.075987119159553 26.26300218786706 22.2963080459428 64-65 22.34036112054941 29.135845719127307 26.27495335737249 22.248839802950794 66-67 22.311945313817013 29.139725604407623 26.27954264439072 22.268786437384644 68-69 22.313449698955573 29.207099154857165 26.24000116489464 22.239449981292626 70-71 22.34708358411632 29.24210922915836 26.18655663282571 22.224250553899612 72-73 22.314969834333752 29.23704510197782 26.167217866785414 22.280767196903014 74-75 22.341139465618664 29.258429567184823 26.159849086534127 22.240581880662386 76-77 22.304400645451942 29.338937041099722 26.143044092214616 22.21361822123372 78-79 22.36719649322157 29.381122939768478 26.066074922535183 22.18560564447477 80-81 22.308545798503907 29.381532374517434 26.10163381432588 22.20828801265278 82-83 22.348431151439723 29.464969812786602 26.03976930347775 22.146829732295927 84-85 22.317044703786156 29.476396370929315 26.044530320600746 22.162028604683783 86-87 22.293798057027928 29.535289347237214 26.007359559170368 22.16355303656449 88-89 22.296015439646705 29.565322784216058 26.0207128116036 22.11794896453364 90-91 22.27917709205094 29.591837828825152 25.984493140021197 22.14449193910271 92-93 22.279166410002333 29.646293228237937 25.96384225461027 22.110698107149464 94-95 22.233115439219116 29.697270633707973 25.930282101151676 22.139331825921236 96-97 22.22005964873762 29.715685043134854 25.92812783352365 22.136127474603878 98-99 22.21446050035694 29.7561165079752 25.876637196351055 22.152785795316802 100-101 22.277982769812226 29.78896424360243 25.829724894374444 22.1033280922109 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 254363.0 1 139890.5 2 18425.5 3 10192.5 4 8410.0 5 7400.5 6 7028.0 7 6970.5 8 6466.5 9 6029.0 10 6277.5 11 7812.0 12 16564.0 13 15880.0 14 7465.0 15 7454.5 16 7932.5 17 9174.5 18 11258.5 19 12664.5 20 14761.5 21 18647.5 22 23653.0 23 29680.0 24 36962.5 25 45204.0 26 55329.0 27 66625.5 28 75479.5 29 84361.0 30 97303.0 31 113244.5 32 128320.0 33 143080.0 34 159339.0 35 173680.5 36 188320.5 37 204643.5 38 218499.5 39 226433.0 40 239793.5 41 258501.5 42 272243.0 43 285764.0 44 296936.0 45 308218.0 46 318616.0 47 326466.5 48 337392.5 49 345492.5 50 343902.5 51 341058.0 52 341609.0 53 335571.5 54 318815.0 55 297820.5 56 270740.5 57 241620.5 58 224218.5 59 205919.5 60 176157.0 61 146186.5 62 117372.5 63 90375.0 64 70958.0 65 55090.5 66 42826.5 67 35790.0 68 28893.0 69 22709.5 70 18482.5 71 16454.5 72 15263.0 73 12309.5 74 9323.5 75 7447.0 76 5732.0 77 3925.0 78 2650.5 79 1814.0 80 1255.0 81 1012.5 82 834.5 83 654.5 84 493.0 85 377.5 86 282.5 87 189.5 88 110.5 89 70.5 90 52.0 91 38.0 92 24.0 93 13.5 94 8.0 95 3.5 96 1.0 97 2.0 98 2.5 99 1.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.012558981979413332 2 0.01794446189044906 3 0.005931522605792828 4 6.2098973129239E-4 5 8.672442799083376E-4 6 0.0 7 0.0 8 0.00746258349501372 9 0.010021489456718567 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 1.0706719505041206E-4 24-25 0.004716309941970651 26-27 0.007039668074564592 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 5.3533597525206025E-6 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 4.282687802016482E-5 62-63 1.1242055480293266E-4 64-65 3.051415058936743E-4 66-67 2.087810303483035E-4 68-69 3.051415058936743E-4 70-71 1.552474328230975E-4 72-73 6.424031703024724E-5 74-75 7.494703653528844E-5 76-77 0.12399987194763472 78-79 0.05575524182250208 80-81 6.424031703024724E-5 82-83 0.0022805312545737767 84-85 0.009625340835032043 86-87 0.11749018648856965 88-89 0.13397853452633313 90-91 0.14704608568223593 92-93 0.14502786905553566 94-95 0.11214218009580158 96-97 1.201722197245825 98-99 0.07592670136999971 100-101 0.06818574316785492 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 9339929.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.01206895892686 #Duplication Level Percentage of deduplicated Percentage of total 1 89.88071352379427 58.43331145686464 2 5.598706895902293 7.279670375944384 3 1.4955360622236962 2.916836808235466 4 0.764425623881389 1.987875654949902 5 0.4656029636970562 1.5134905991676868 6 0.3000954517260797 1.170589572112572 7 0.21203078364057198 0.9649191949219318 8 0.1576022687742053 0.8196839652505568 9 0.13077363447937732 0.7651678088504494 >10 0.9523498746818442 11.950682669875244 >50 0.03832935852970367 1.6074042639330184 >100 0.003267452753360959 0.32035735642618596 >500 2.0585669678670283E-4 0.09107247026488428 >1k 5.1464174194782216E-5 0.07230257466438943 >5k 1.5439252258434667E-4 0.7529036731479662 >10k+ 1.5439252258434667E-4 9.353731555390725 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 237249 2.540158495851521 No Hit AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 21184 0.22681114599479288 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 21134 0.22627581001954086 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 1.0706719505041205E-5 0.0 0.0 0.0 0.0 2 2.141343901008241E-5 0.0 0.0 0.0 0.0 3 4.282687802016482E-5 0.0 0.0 0.0 0.0 4 5.353359752520603E-5 0.0 0.0 0.0 0.0 5 7.494703653528844E-5 0.0 0.0 0.0 0.0 6 7.494703653528844E-5 1.0706719505041205E-5 0.0 0.0 0.0 7 8.565375604032964E-5 1.0706719505041205E-5 0.0 0.0 1.0706719505041205E-5 8 8.565375604032964E-5 1.0706719505041205E-5 0.0 1.0706719505041205E-5 1.0706719505041205E-5 9 9.636047554537084E-5 1.0706719505041205E-5 0.0 1.0706719505041205E-5 1.0706719505041205E-5 10-11 9.636047554537084E-5 1.0706719505041205E-5 0.0 1.0706719505041205E-5 1.0706719505041205E-5 12-13 1.0706719505041206E-4 2.141343901008241E-5 0.0 2.6766798762603014E-5 1.0706719505041205E-5 14-15 1.2848063406049447E-4 2.141343901008241E-5 0.0 3.212015851512362E-5 2.141343901008241E-5 16-17 1.3918735356553566E-4 2.141343901008241E-5 0.0 3.212015851512362E-5 3.212015851512362E-5 18-19 1.5524743282309747E-4 2.141343901008241E-5 0.0 4.818023777268543E-5 3.212015851512362E-5 20-21 1.9272095109074168E-4 2.141343901008241E-5 0.0 5.888695727772663E-5 3.212015851512362E-5 22-23 2.034276705957829E-4 2.141343901008241E-5 0.0 7.494703653528844E-5 3.747351826764422E-5 24-25 2.516079083684683E-4 2.6766798762603014E-5 0.0 7.494703653528844E-5 5.353359752520603E-5 26-27 2.9443478638863313E-4 3.212015851512362E-5 0.0 7.494703653528844E-5 6.424031703024724E-5 28-29 3.3190830465627737E-4 3.212015851512362E-5 0.0 7.494703653528844E-5 6.424031703024724E-5 30-31 3.8008854242896275E-4 4.282687802016482E-5 0.0 7.494703653528844E-5 6.424031703024724E-5 32-33 4.122087009440864E-4 4.282687802016482E-5 0.0 8.030039628780905E-5 6.424031703024724E-5 34-35 4.764490179743336E-4 4.282687802016482E-5 0.0 9.100711579285023E-5 6.424031703024724E-5 36-37 5.621027740146632E-4 4.282687802016482E-5 0.0 9.636047554537084E-5 7.494703653528844E-5 38-39 6.209897312923899E-4 4.282687802016482E-5 0.0 9.636047554537084E-5 7.494703653528844E-5 40-41 6.905834080751577E-4 4.282687802016482E-5 0.0 1.0171383529789145E-4 8.030039628780905E-5 42-43 7.387636458478432E-4 4.282687802016482E-5 0.0 1.0706719505041206E-4 9.636047554537086E-5 44-45 7.976506031255698E-4 4.282687802016482E-5 0.0 1.0706719505041206E-4 1.1242055480293266E-4 46-47 8.993644384234613E-4 4.282687802016482E-5 0.0 1.1242055480293266E-4 1.1777391455545326E-4 48-49 9.743114749587497E-4 4.282687802016482E-5 0.0 1.3383399381301507E-4 1.3383399381301507E-4 50-51 0.001038551791988997 4.282687802016482E-5 0.0 1.3918735356553566E-4 1.3918735356553566E-4 52-53 0.0010760253102566411 4.282687802016482E-5 0.0 1.3918735356553566E-4 1.6595415232813869E-4 54-55 0.0011349122675343678 4.282687802016482E-5 0.0 1.4989407307057688E-4 1.9272095109074168E-4 56-57 0.0012312727430797386 4.282687802016482E-5 0.0 1.606007925756181E-4 1.9272095109074168E-4 58-59 0.0012848063406049447 4.282687802016482E-5 0.0 1.606007925756181E-4 2.301944693583859E-4 60-61 0.0013436932978826712 5.353359752520603E-5 0.0 1.606007925756181E-4 2.3554782911090652E-4 62-63 0.001456113852685604 5.888695727772663E-5 0.0 1.606007925756181E-4 2.5696126812098895E-4 64-65 0.0015417676087259337 6.424031703024724E-5 0.0 1.606007925756181E-4 2.6766798762603014E-4 66-67 0.001616714645261222 6.424031703024724E-5 0.0 1.606007925756181E-4 2.6766798762603014E-4 68-69 0.0017612553585792782 6.424031703024724E-5 0.0 1.766608718331799E-4 2.7302134737855076E-4 70-71 0.0018415557548670872 6.424031703024724E-5 0.0 1.820142315857005E-4 2.8372806688359194E-4 72-73 0.0019379162304124582 7.494703653528844E-5 0.0 1.820142315857005E-4 2.9443478638863313E-4 74-75 0.002050336785215391 7.494703653528844E-5 0.0 1.820142315857005E-4 2.9978814614115375E-4 76-77 0.002248411096058653 7.494703653528844E-5 0.0 1.873675913382211E-4 3.1049486564619494E-4 78-79 0.002446485406901916 7.494703653528844E-5 0.0 1.9272095109074168E-4 3.212015851512362E-4 80-81 0.0027676869920531515 7.494703653528844E-5 0.0 1.9807431084326228E-4 3.3190830465627737E-4 82-83 0.0032709028087900883 7.494703653528844E-5 0.0 2.034276705957829E-4 3.37261664408798E-4 84-85 0.003886539180329957 7.494703653528844E-5 0.0 2.034276705957829E-4 3.533217436663598E-4 86-87 0.004630656185930322 7.494703653528844E-5 0.0 2.034276705957829E-4 3.64028463171401E-4 88-89 0.005717388215692004 8.565375604032964E-5 0.0 2.0878103034830352E-4 3.747351826764422E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AAGCAGT 10770 0.0 27.389631 1 AGTGGTA 12015 0.0 23.717514 5 GGTATCA 12660 0.0 22.097176 8 GTATCAA 12865 0.0 21.669846 9 TGGTATC 13780 0.0 20.473225 7 ATCAACG 11325 0.0 20.28137 10-11 GTGGTAT 14100 0.0 20.21035 6 AGCAGTG 14680 0.0 19.996447 2 CAACGCA 11510 0.0 19.79064 12-13 GCAGTGG 15420 0.0 18.850485 3 ACGCAGA 12970 0.0 17.617685 14-15 CAGTGGT 17150 0.0 17.003336 4 AGAGTAC 13365 0.0 16.690243 18-19 TCAACGC 11150 0.0 15.518857 10-11 AGTACTT 10855 0.0 15.287638 20-21 AACGCAG 11935 0.0 14.478276 12-13 CGTCACG 565 2.0008883E-11 14.267467 3 GCAGAGT 16535 0.0 13.905303 16-17 CGCAGAG 13150 0.0 13.21265 14-15 CGCGTAG 275 2.8358045E-9 12.92916 54-55 >>END_MODULE