FastQCFastQC Report
Sat 18 Jun 2016
SRR578601_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR578601_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8433580
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1361161.6139765081969935No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT133930.15880563177203513No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT116930.1386481185925787No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCACG3800.024.999283
CGTACGC2300.021.68364520-21
CAACGCA86550.020.46963112-13
CTCGCGT2450.020.35818752-53
TCAACGC85000.020.34004810-11
ACTCGCG2350.020.2144650-51
ATCAACG88800.020.03126510-11
ACGTACG2750.018.99900220-21
AACGCAG91850.018.92649312-13
CGCGTAG2650.018.82077254-55
TCGCGTA3050.017.91075552-53
GGTATCA100000.017.8592748
ACGCAGA101850.017.39466514-15
GTATCAA106850.016.7142439
AAGCAGT116150.016.5851691
AGTGGTA108900.016.5306475
CGCAGAG105400.016.51587514-15
TATCAAC111200.015.9322619
TGGTATC116800.015.2091447
CGTAGCG3150.015.07884256-57
Produced by FastQC (version 0.11.5)