##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR578601_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 8433580 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.497754690179022 31.0 31.0 34.0 30.0 34.0 2 31.812435999895655 31.0 31.0 34.0 30.0 34.0 3 31.888128173326155 31.0 31.0 34.0 30.0 34.0 4 35.55496206830314 37.0 35.0 37.0 33.0 37.0 5 35.3734327533503 37.0 35.0 37.0 33.0 37.0 6 35.371927698557435 37.0 35.0 37.0 32.0 37.0 7 35.3494718731547 37.0 35.0 37.0 32.0 37.0 8 35.33637150533937 37.0 35.0 37.0 32.0 37.0 9 36.90555363202815 39.0 37.0 39.0 33.0 39.0 10-11 36.876548334159395 39.0 37.0 39.0 32.5 39.0 12-13 36.858464495504876 39.0 37.0 39.0 32.0 39.0 14-15 38.04510148715018 40.0 37.0 41.0 33.0 41.0 16-17 37.99664555265973 40.0 37.0 41.0 33.0 41.0 18-19 37.92267649088525 40.0 37.0 41.0 33.0 41.0 20-21 37.86093639948871 40.0 37.0 41.0 32.0 41.0 22-23 37.77316934208248 40.0 37.0 41.0 32.0 41.0 24-25 37.691975531150476 39.5 36.5 41.0 32.0 41.0 26-27 37.512120356953986 39.0 36.0 41.0 32.0 41.0 28-29 37.34395825971889 39.0 36.0 41.0 31.5 41.0 30-31 37.21471877897642 39.0 36.0 41.0 31.0 41.0 32-33 37.08601335375961 39.0 36.0 41.0 31.0 41.0 34-35 36.93484967238112 39.0 36.0 40.0 30.0 41.0 36-37 36.78633119031301 39.0 35.0 40.0 30.0 41.0 38-39 36.619667626322396 38.5 35.0 40.0 30.0 41.0 40-41 36.453694279297764 38.0 35.0 40.0 30.0 41.0 42-43 36.3944746477771 38.0 35.0 40.0 30.0 41.0 44-45 36.432763962635086 38.0 35.0 40.0 30.0 41.0 46-47 36.30135067195663 38.0 35.0 40.0 30.0 41.0 48-49 36.155911783607905 38.0 35.0 40.0 29.5 41.0 50-51 35.95168421951295 38.0 34.5 40.0 28.5 41.0 52-53 36.00529531942544 38.0 35.0 40.0 29.0 41.0 54-55 35.98616821089027 38.0 35.0 40.0 28.5 41.0 56-57 35.814564099706175 38.0 34.0 40.0 28.0 41.0 58-59 35.60146379117765 38.0 34.0 40.0 28.0 41.0 60-61 35.33241642339315 38.0 34.0 40.0 28.0 41.0 62-63 35.04086870581651 37.0 34.0 40.0 27.5 41.0 64-65 34.706660813082934 37.0 33.0 40.0 27.0 41.0 66-67 34.35377781440385 36.0 33.0 39.0 26.0 41.0 68-69 33.97784564799291 36.0 33.0 39.0 26.0 40.5 70-71 33.581998214281484 35.0 32.0 39.0 26.0 40.0 72-73 33.18366725637274 35.0 32.0 38.0 26.0 40.0 74-75 32.76910297880615 35.0 31.5 37.0 25.0 39.5 76-77 31.425892325679012 34.0 30.0 36.0 23.0 39.0 78-79 32.06634477884836 34.5 31.0 36.0 25.0 39.0 80-81 31.94723628636949 35.0 31.0 36.0 24.5 38.0 82-83 31.643545979287563 34.0 31.0 35.5 24.0 37.0 84-85 31.325449690404312 34.0 31.0 35.0 23.5 37.0 86-87 30.987548763395854 34.0 31.0 35.0 22.5 36.0 88-89 30.675898491506572 34.0 31.0 35.0 20.0 36.0 90-91 30.361108805513197 34.0 30.0 35.0 19.5 36.0 92-93 30.00530504246121 34.0 30.0 35.0 18.0 35.0 94-95 29.63781460542261 34.0 30.0 35.0 14.0 35.0 96-97 29.250258016168697 34.0 29.5 35.0 4.5 35.0 98-99 28.7746052684625 34.0 29.0 35.0 2.0 35.0 100-101 27.652738042444607 33.0 27.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.37721105138940914 1101 2 0.38094912466299746 1101 3 0.3566818062402497 1101 4 0.31768792618724007 1101 5 0.32840669224854224 1101 6 0.3066153474856179 1101 7 0.3171740524460347 1101 8 0.256394886842763 1101 9 0.38619481624894547 1101 10-11 0.35814485212818425 1101 12-13 0.3607201445705783 1101 14-15 0.5052147476355273 1101 16-17 0.45736067352531506 1101 18-19 0.4105031389305793 1101 20-21 0.42442916943743114 1101 22-23 0.5310636834644598 1101 24-25 0.5369267059392868 1101 26-27 0.48778273500302305 1101 28-29 0.552497739224016 1101 30-31 0.5299611412117855 1101 32-33 0.5082746107147358 1101 34-35 0.5369316780522624 1101 36-37 0.5337340418924228 1101 38-39 0.5109664470595945 1101 40-41 0.4833808439569367 1101 42-43 0.5280008410837738 1101 44-45 0.48194964640670435 1101 46-47 0.44131530226008664 1101 48-49 0.48325674190531487 1101 50-51 0.47531307939551937 1101 52-53 0.5075766065915204 1101 54-55 0.5345307837133575 1101 56-57 0.6196008168076688 1101 58-59 0.5467823259151103 1101 60-61 0.5236987739003283 1101 62-63 0.5264490374936202 1101 64-65 0.5125579735744239 1101 66-67 0.49639285342850314 1101 68-69 0.5512564350240297 1101 70-71 0.5977822943866187 1101 72-73 0.50403944173474 1101 74-75 0.5133005279164209 1101 76-77 0.40949060604594223 1101 78-79 0.519816959597911 1101 80-81 0.5499478913274665 1101 82-83 0.5712194173099192 1101 84-85 0.6541492533169908 1101 86-87 0.552645199796963 1101 88-89 0.508364060569864 1101 90-91 0.5555481794573822 1101 92-93 0.5843871521985342 1101 94-95 0.6742371090748129 1101 96-97 0.4440434891142502 1101 98-99 0.5131247810673187 1101 100-101 0.6378020055207578 1102 1 0.24916081215496177 1102 2 0.24952142356383433 1102 3 0.307462714609418 1102 4 0.22800568133173726 1102 5 0.3028563150350365 1102 6 0.18531116947990256 1102 7 0.23440100251460194 1102 8 0.2145242942121186 1102 9 0.4133901160455835 1102 10-11 0.3260279191265667 1102 12-13 0.24005380127020004 1102 14-15 0.4379677375354518 1102 16-17 0.3824016426901835 1102 18-19 0.44959913201888924 1102 20-21 0.4465188197348269 1102 22-23 0.37377321526968643 1102 24-25 0.3505835276236482 1102 26-27 0.3861611627211019 1102 28-29 0.41675240822288373 1102 30-31 0.3806789506988011 1102 32-33 0.4658155374245112 1102 34-35 0.46409041339994417 1102 36-37 0.4308488999013562 1102 38-39 0.47785240312706634 1102 40-41 0.3913875826415989 1102 42-43 0.38762477386887184 1102 44-45 0.4520141887620355 1102 46-47 0.3253852491487592 1102 48-49 0.41192658668949633 1102 50-51 0.41271994353497377 1102 52-53 0.4154270043957453 1102 54-55 0.44540836232958014 1102 56-57 0.5010711322065546 1102 58-59 0.49591786061577636 1102 60-61 0.404641743973734 1102 62-63 0.30552252407568403 1102 64-65 0.38372831189906265 1102 66-67 0.36623227765252864 1102 68-69 0.4181090903778255 1102 70-71 0.37653662480611416 1102 72-73 0.42973122094277727 1102 74-75 0.30962800315449357 1102 76-77 0.26855200884712715 1102 78-79 0.36058122557057715 1102 80-81 0.4098790397801899 1102 82-83 0.35937853263068575 1102 84-85 0.31488744655558776 1102 86-87 0.33466907654133493 1102 88-89 0.47460260300160684 1102 90-91 0.5843106824823074 1102 92-93 0.4720601301685825 1102 94-95 0.3562063779556546 1102 96-97 0.33687746741120606 1102 98-99 0.4840246008454514 1102 100-101 0.6259373990582411 1103 1 0.19525690917350857 1103 2 0.25571783633285605 1103 3 0.2758292234231128 1103 4 0.1762187569433067 1103 5 0.2482099136315341 1103 6 0.1349002210772312 1103 7 0.16496799111088478 1103 8 0.11747579054777191 1103 9 0.30446967763295163 1103 10-11 0.2648930938604366 1103 12-13 0.2401518038499475 1103 14-15 0.3915590152257238 1103 16-17 0.3325630237439299 1103 18-19 0.31391826650840216 1103 20-21 0.30888996931390267 1103 22-23 0.3276145678174771 1103 24-25 0.32957174380842247 1103 26-27 0.3189757399170361 1103 28-29 0.3840955393211587 1103 30-31 0.36649749126970477 1103 32-33 0.36025802454781797 1103 34-35 0.3722108580095167 1103 36-37 0.47374022040933284 1103 38-39 0.4764814287720114 1103 40-41 0.4389151143148098 1103 42-43 0.390760155257567 1103 44-45 0.2700486734364773 1103 46-47 0.33000006482030386 1103 48-49 0.37260687123620784 1103 50-51 0.41089151028610615 1103 52-53 0.4026009067548273 1103 54-55 0.3662904910556577 1103 56-57 0.39207440056187437 1103 58-59 0.37666650389840584 1103 60-61 0.44968363880780515 1103 62-63 0.3075710357533268 1103 64-65 0.38783606638991586 1103 66-67 0.32695700258955185 1103 68-69 0.30394854564481477 1103 70-71 0.31640051634458644 1103 72-73 0.3058696094534312 1103 74-75 0.29796902168148875 1103 76-77 0.2585991443751574 1103 78-79 0.3059460294225076 1103 80-81 0.3886427835802344 1103 82-83 0.324578227911843 1103 84-85 0.413730874546399 1103 86-87 0.43527757934024436 1103 88-89 0.46203530803040493 1103 90-91 0.4465992417161715 1103 92-93 0.3267149455302807 1103 94-95 0.4376587486751866 1103 96-97 0.46629403420106996 1103 98-99 0.5225360220591497 1103 100-101 0.7536316018944653 1104 1 0.12354963227124216 1104 2 0.14442712913380262 1104 3 0.20806146568299155 1104 4 0.14339401500900095 1104 5 0.20520638685302828 1104 6 0.18110951742383463 1104 7 0.15368705951954098 1104 8 0.15515170871096018 1104 9 0.203534751939479 1104 10-11 0.24228628864523216 1104 12-13 0.2290569379983438 1104 14-15 0.22144944709692282 1104 16-17 0.2325849118409451 1104 18-19 0.23989117270834726 1104 20-21 0.299524831733315 1104 22-23 0.3311586387305425 1104 24-25 0.2641378880124634 1104 26-27 0.2047026070955127 1104 28-29 0.25606101691801797 1104 30-31 0.21049588943886732 1104 32-33 0.2250650847199296 1104 34-35 0.2837360067394812 1104 36-37 0.34704099943795796 1104 38-39 0.35946620090464876 1104 40-41 0.33934842238787155 1104 42-43 0.287589948967792 1104 44-45 0.37633331443700513 1104 46-47 0.34373041525352477 1104 48-49 0.16142791017603741 1104 50-51 0.2426455466798103 1104 52-53 0.263050691144322 1104 54-55 0.271487609035276 1104 56-57 0.28196791071507477 1104 58-59 0.2944686128872078 1104 60-61 0.30646452559373216 1104 62-63 0.27217231502424966 1104 64-65 0.3381420189699256 1104 66-67 0.3159835739091932 1104 68-69 0.34458128262097176 1104 70-71 0.21272263684031145 1104 72-73 0.3205968298575428 1104 74-75 0.35662906964715546 1104 76-77 0.3184671753625743 1104 78-79 0.2753549676589202 1104 80-81 0.37816062700492736 1104 82-83 0.3704337456269542 1104 84-85 0.34883699974337645 1104 86-87 0.3974462377322254 1104 88-89 0.3509636119174466 1104 90-91 0.5282464562073059 1104 92-93 0.5259078704208626 1104 94-95 0.528829165544547 1104 96-97 0.5450692394241017 1104 98-99 0.5640984696834295 1104 100-101 0.5886654763334285 1105 1 0.25133639114912754 1105 2 0.19389545721705304 1105 3 0.16063731717844476 1105 4 0.1698149648228764 1105 5 0.1806402521674002 1105 6 0.22962699659643704 1105 7 0.23536919964310954 1105 8 0.19974193222977732 1105 9 0.19087034146524218 1105 10-11 0.1831891734882376 1105 12-13 0.19078639990656399 1105 14-15 0.2593362384519864 1105 16-17 0.13047542853641403 1105 18-19 0.2076598725810257 1105 20-21 0.21673513135052502 1105 22-23 0.23436988886214039 1105 24-25 0.21052241775458214 1105 26-27 0.24518380540723683 1105 28-29 0.21561198231432144 1105 30-31 0.23435309684820282 1105 32-33 0.31916901985916013 1105 34-35 0.3029772760180194 1105 36-37 0.30279296134671085 1105 38-39 0.40951170400258974 1105 40-41 0.41543877921385786 1105 42-43 0.31987371955099775 1105 44-45 0.2346452857282486 1105 46-47 0.20057002342770858 1105 48-49 0.23189160045330226 1105 50-51 0.2469612199940201 1105 52-53 0.20862394214946534 1105 54-55 0.35557901036405326 1105 56-57 0.39175413765281064 1105 58-59 0.2878443138525384 1105 60-61 0.22989557254461346 1105 62-63 0.2676282310631919 1105 64-65 0.2491792097193084 1105 66-67 0.2274391406572036 1105 68-69 0.21084985649640942 1105 70-71 0.23551229658482953 1105 72-73 0.2481420871125124 1105 74-75 0.2858755776701756 1105 76-77 0.2524515301470096 1105 78-79 0.3360922336894774 1105 80-81 0.3192989226854728 1105 82-83 0.26228739698898096 1105 84-85 0.37809576491444474 1105 86-87 0.43644696385642945 1105 88-89 0.42929601211916335 1105 90-91 0.44563015612327916 1105 92-93 0.4055901601378942 1105 94-95 0.5104554614920183 1105 96-97 0.5702777376258652 1105 98-99 0.7130471023708722 1105 100-101 0.7668734255343956 1106 1 -0.04686133957101646 1106 2 -0.01600391776700505 1106 3 -0.05605645506215495 1106 4 0.04599004727035805 1106 5 0.007771634711531306 1106 6 0.099264243560647 1106 7 0.12328440741811875 1106 8 0.06583333895519417 1106 9 -0.041680892847438145 1106 10-11 0.01566969415505781 1106 12-13 0.014666503411035592 1106 14-15 0.2202569320682528 1106 16-17 0.12435285831084286 1106 18-19 0.03022367033779716 1106 20-21 0.06992813798563446 1106 22-23 0.06364179257854374 1106 24-25 0.22268544281067193 1106 26-27 0.21357422030857265 1106 28-29 0.1670922556165877 1106 30-31 0.10682159245484257 1106 32-33 0.13959592794111586 1106 34-35 0.07261009601388935 1106 36-37 0.08038237947820903 1106 38-39 0.12114268842059772 1106 40-41 0.09248903077415349 1106 42-43 -0.0020235415044425054 1106 44-45 -0.046625900809047494 1106 46-47 0.016530874937132012 1106 48-49 0.12069345082358751 1106 50-51 0.09835398381453331 1106 52-53 -0.13522515105665178 1106 54-55 -0.1502182238029377 1106 56-57 -0.10294235171646449 1106 58-59 0.02333206540082955 1106 60-61 0.07550875370962018 1106 62-63 0.0381967220724988 1106 64-65 0.019000912416650806 1106 66-67 2.216485530937007E-5 1106 68-69 -0.01802453466895315 1106 70-71 -0.08000233547421232 1106 72-73 -0.05738588208532036 1106 74-75 -0.10051521457385348 1106 76-77 -0.14057941358029424 1106 78-79 -0.07380964811664015 1106 80-81 0.07350532078452332 1106 82-83 0.08785581376714546 1106 84-85 0.1932316132141274 1106 86-87 0.3328877556079348 1106 88-89 0.2142696031906972 1106 90-91 0.2825343722969613 1106 92-93 0.23188805316868155 1106 94-95 0.3217548280653517 1106 96-97 0.3214388977318734 1106 98-99 0.3892963429427745 1106 100-101 0.27717420331917353 1107 1 0.10701484676383188 1107 2 0.12483179761059304 1107 3 0.06059346845173508 1107 4 0.1170345296901516 1107 5 0.0076446439038804215 1107 6 -0.04529913500351057 1107 7 0.03822296563743777 1107 8 0.04014883536860481 1107 9 -0.09213915481971213 1107 10-11 -0.06069501369314878 1107 12-13 -0.08632082947544006 1107 14-15 -0.034284607982783655 1107 16-17 0.09933147505891782 1107 18-19 0.018602084752508574 1107 20-21 0.01592900950248577 1107 22-23 -0.03996309229345485 1107 24-25 -0.0234975882317201 1107 26-27 -0.07948438927171253 1107 28-29 0.03423576103551085 1107 30-31 0.05886956727257342 1107 32-33 0.01684765393283527 1107 34-35 -0.04188681625095825 1107 36-37 -0.010954746435793083 1107 38-39 0.01887984434369372 1107 40-41 -0.009089230748294597 1107 42-43 0.023291595641239837 1107 44-45 0.05273482518464334 1107 46-47 0.09114094310232446 1107 48-49 0.04434087322541558 1107 50-51 0.015693145382400076 1107 52-53 0.006441797731717713 1107 54-55 -0.023128388481929107 1107 56-57 0.04949317246585139 1107 58-59 0.06639641243864247 1107 60-61 0.20003185171754723 1107 62-63 0.10953068560213808 1107 64-65 0.07800027282284105 1107 66-67 0.028962864001506716 1107 68-69 0.10489469656914707 1107 70-71 0.06271753998697704 1107 72-73 0.021673766253293536 1107 74-75 0.05678090275920766 1107 76-77 0.06265097226742355 1107 78-79 0.1078849238264965 1107 80-81 0.18519252374821704 1107 82-83 0.16764437806994437 1107 84-85 0.14516135905255823 1107 86-87 0.17928608908589894 1107 88-89 0.2174016193296069 1107 90-91 0.18291579821171666 1107 92-93 0.3442105509448332 1107 94-95 0.26697254154375827 1107 96-97 0.1623454259834105 1107 98-99 0.27335508554294563 1107 100-101 0.428673823991641 1108 1 -0.01213676254327467 1108 2 0.002288183600896332 1108 3 -0.018050483522351612 1108 4 0.010407063489928703 1108 5 0.005069633506899152 1108 6 -0.03116793758285752 1108 7 0.02656125415663979 1108 8 0.015020661876022245 1108 9 -0.13145708684708524 1108 10-11 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-1.0804922960312418 2316 96-97 -1.15994618991472 2316 98-99 -1.313229123265934 2316 100-101 -1.7369135130816034 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 145.0 3 83.0 4 274.0 5 586.0 6 1086.0 7 1890.0 8 3338.0 9 5034.0 10 7177.0 11 9295.0 12 11657.0 13 14276.0 14 16660.0 15 19145.0 16 22590.0 17 26779.0 18 31549.0 19 36725.0 20 39592.0 21 40872.0 22 45442.0 23 53355.0 24 66136.0 25 82903.0 26 105987.0 27 134502.0 28 168258.0 29 209244.0 30 255230.0 31 309990.0 32 377615.0 33 469569.0 34 605462.0 35 854590.0 36 1098582.0 37 1569146.0 38 1437834.0 39 300395.0 40 587.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 17.84187384638476 30.487479920088838 28.715887900185148 22.95475833334125 2 22.435039449439028 29.841372228638374 27.31624055264787 20.407347769274732 3 21.821480320338456 28.651023645948698 27.703466380825226 21.82402965288762 4 21.990758582188928 28.4217708338027 27.98961692274162 21.59785366126675 5 22.393201917815205 28.562083249590476 28.25530870433643 20.78940612825789 6 22.434670034823043 28.59574487258902 27.180341343613613 21.789243748974325 7 22.56395535039157 28.65262778708778 27.84673031461495 20.9366865479057 8 21.78796684866951 28.84173104432193 27.750152723023042 21.62014938398552 9 21.322780564727584 28.596156329366757 28.405536788587206 21.675526317318454 10-11 21.401312599294382 29.04646625691144 27.73395589282666 21.818265250967517 12-13 21.359036461155632 29.219851882558036 27.53102538448094 21.890086271805394 14-15 21.56342985798616 29.168913389622997 27.39919627247244 21.868460479918404 16-17 21.74524728737629 28.966304161394834 27.38414067053505 21.904307880693825 18-19 21.727950747366524 29.0494860456346 27.38914715868867 21.833416048310212 20-21 21.822301392577188 28.934578373197027 27.460878972753182 21.782241261472603 22-23 21.69068092366379 28.898799813815423 27.538859152886268 21.87166010963452 24-25 21.710038915857798 28.79040692090429 27.53315318049986 21.966400982738055 26-27 21.97565102694532 28.657096313611557 27.648426151671423 21.7188265077717 28-29 21.918860660696698 28.76100218448784 27.566761349150692 21.753375805664774 30-31 21.883289895250584 28.74177383897001 27.55282529353314 21.822110972246268 32-33 21.881523230905856 28.798572085894207 27.45902516499407 21.860879518205863 34-35 21.869466306170512 28.80990855346768 27.48316784770184 21.83745729265997 36-37 21.873742004708095 28.814158723300267 27.465309121643454 21.846790150348188 38-39 21.874073077605566 28.822711698180818 27.457321476710728 21.84589374750289 40-41 21.8859763929827 28.871950740502157 27.409355593111712 21.832717273403436 42-43 21.91110991482935 28.85892861822303 27.35874583611933 21.871215630828292 44-45 21.875488007220696 28.88922116033695 27.3878693543975 21.847421478044847 46-47 21.938296377339668 28.900007417227215 27.32862247505224 21.833073730380885 48-49 21.94338272939345 28.92378211739497 27.288135844452064 21.844699308759516 50-51 21.965877651945505 28.877736019903693 27.31864802199263 21.837738306158172 52-53 21.89467798674344 28.953865291560753 27.275681104922977 21.87577561677283 54-55 21.95373512719994 28.976151345044222 27.184549600506568 21.885563927249272 56-57 21.93595128047638 28.989207430296503 27.189485366831168 21.885355922395945 58-59 21.93779494829921 29.087342610497767 27.123970135798274 21.85089230540475 60-61 21.936333172348316 29.0720029899815 27.1267482516446 21.86491558602558 62-63 21.899744110088452 29.107170626890266 27.108832924181804 21.88425233883948 64-65 21.902905879898455 29.132463200351786 27.12185401181248 21.842776907937278 66-67 21.89135752199451 29.148827397133903 27.106425077676597 21.853390003194985 68-69 21.885043833809718 29.183661140037742 27.127362223152506 21.803932803000034 70-71 21.893744481816647 29.2392157829945 27.052088330774883 21.814951404413975 72-73 21.877289270874414 29.212382914429618 27.05903647916454 21.851291335531428 74-75 21.937203734570364 29.231903804896813 27.041379010229054 21.78951345030377 76-77 21.87498666046922 29.304186359766554 27.056694784421325 21.764132195342903 78-79 21.904618204843022 29.275971769995664 26.99700483068875 21.822405194472573 80-81 21.869014063380874 29.307193116746117 27.001058092898862 21.822734726974147 82-83 21.902185464327797 29.37596063348081 26.94617488161142 21.775679020579975 84-85 21.887616249729653 29.3775896512603 26.944402416628403 21.790391682381642 86-87 21.844291426546476 29.432242544745048 26.921411605227263 21.802054423481213 88-89 21.831681210646636 29.46630282161729 26.92795653400654 21.77405943372953 90-91 21.81529733930781 29.467945157302594 26.92213950289075 21.79461800049884 92-93 21.812078587202333 29.5136108982045 26.90015196472749 21.77415854986568 94-95 21.819439564795527 29.551655156492167 26.90253443596074 21.726370842751567 96-97 21.786639211079528 29.566583217713077 26.869072967222742 21.777704603984656 98-99 21.77874639239801 29.614374915516308 26.835809940736908 21.771068751348775 100-101 21.848891200284008 29.612493106426108 26.74825836101138 21.7903573322785 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 146034.0 1 82113.5 2 13292.5 3 7642.5 4 6508.5 5 5538.5 6 5060.0 7 5028.5 8 4783.0 9 4479.0 10 4285.5 11 5121.0 12 10358.0 13 10423.0 14 6220.5 15 6425.0 16 6901.5 17 7933.0 18 9667.0 19 11101.0 20 13180.0 21 17124.0 22 21539.5 23 26553.0 24 32608.0 25 40637.5 26 51522.5 27 63890.0 28 74867.5 29 85494.5 30 99804.0 31 117056.0 32 134633.0 33 152486.0 34 169996.5 35 187669.0 36 207250.5 37 225898.0 38 239335.0 39 244869.0 40 252634.5 41 263889.0 42 271157.5 43 282949.5 44 295725.0 45 304995.0 46 310923.5 47 313296.0 48 320449.0 49 326164.0 50 323678.0 51 316354.5 52 306648.5 53 297658.5 54 282295.0 55 258216.0 56 231935.0 57 207731.5 58 187795.5 59 161541.5 60 129331.0 61 98883.0 62 70402.5 63 48311.0 64 32274.0 65 19852.5 66 11456.5 67 6792.5 68 3841.0 69 1896.0 70 921.5 71 457.5 72 266.5 73 152.5 74 102.0 75 69.0 76 49.0 77 43.0 78 33.0 79 21.0 80 15.0 81 13.0 82 9.5 83 10.0 84 8.5 85 5.0 86 4.5 87 4.0 88 3.0 89 1.5 90 0.5 91 0.5 92 0.0 93 0.0 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1449918065637606 2 0.0 3 0.0 4 0.0028694812878990892 5 2.9643401734494724E-4 6 9.960182982790228E-4 7 1.1857360693797889E-5 8 1.4228832832557467E-4 9 3.20148738732543E-4 10-11 1.0078756589728206E-4 12-13 2.3714721387595777E-5 14-15 5.928680346898944E-6 16-17 1.7786041040696834E-5 18-19 5.928680346898944E-6 20-21 9.485888555038311E-5 22-23 1.600743693662715E-4 24-25 0.0 26-27 5.928680346898944E-6 28-29 0.005715247854410582 30-31 0.0014228832832557468 32-33 2.3121853352905884E-4 34-35 2.13432492488362E-4 36-37 2.7271929595735144E-4 38-39 0.0011086632248701025 40-41 0.0033497043959979035 42-43 4.8615178844571344E-4 44-45 5.572959526085008E-4 46-47 0.006041325273490025 48-49 0.006551191783323335 50-51 0.006794267677546191 52-53 0.009189454537693363 54-55 0.004742944277519156 56-57 0.0 58-59 5.33581231220905E-5 60-61 9.841609375852247E-4 62-63 8.359439289127512E-4 64-65 4.920804687926124E-4 66-67 6.580835185057829E-4 68-69 9.308028144631343E-4 70-71 0.001162021347992193 72-73 0.0025849046312479396 74-75 6.28440116771288E-4 76-77 0.0 78-79 0.0 80-81 1.2450228728487785E-4 82-83 2.0750381214146304E-4 84-85 1.4228832832557467E-4 86-87 0.0025671185902072426 88-89 0.0016185297347034119 90-91 4.920804687926124E-4 92-93 5.217238705271071E-4 94-95 3.20148738732543E-4 96-97 9.60446216197629E-4 98-99 0.0 100-101 1.1857360693797889E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 8433580.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.264129410810575 #Duplication Level Percentage of deduplicated Percentage of total 1 90.45435352732923 56.320615737968375 2 5.073585005191719 6.318047066800103 3 1.3625183511124797 2.54508056814815 4 0.6926346439161799 1.7250517241283088 5 0.4397363673625518 1.3689901042050832 6 0.2758295870038301 1.0304573460322148 7 0.19148824344513252 0.834599413936157 8 0.1444039875678003 0.7192950855486877 9 0.12535072241311615 0.7024368241861972 >10 1.1148535219039981 14.877973124184226 >50 0.11379842543533875 4.688135871704413 >100 0.010953684139460228 0.905134650824107 >500 7.05618827283012E-5 0.03194055741702735 >1k 7.05618827283012E-5 0.1678307143508177 >5k 1.411237654566024E-4 0.6820429344455744 >10k+ 2.1168564818490357E-4 7.082368276120555 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 136116 1.6139765081969935 No Hit AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 13393 0.15880563177203513 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11693 0.1386481185925787 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 3.557208208139367E-5 0.0 0.0 0.0 0.0 2 1.5414568901937255E-4 0.0 0.0 0.0 1.1857360693797889E-5 3 2.4900457456975564E-4 0.0 0.0 0.0 2.3714721387595777E-5 4 2.8457665665114934E-4 0.0 0.0 0.0 3.557208208139367E-5 5 3.438634601201388E-4 0.0 0.0 0.0 4.7429442775191555E-5 6 4.624370670581177E-4 0.0 0.0 1.1857360693797889E-5 5.928680346898944E-5 7 4.624370670581177E-4 0.0 0.0 1.1857360693797889E-5 5.928680346898944E-5 8 5.33581231220905E-4 0.0 0.0 1.1857360693797889E-5 5.928680346898944E-5 9 5.572959526085007E-4 0.0 0.0 1.1857360693797889E-5 5.928680346898944E-5 10-11 5.987967150367934E-4 0.0 0.0 1.1857360693797889E-5 5.928680346898944E-5 12-13 6.46226157811985E-4 0.0 0.0 1.7786041040696834E-5 7.707284450968627E-5 14-15 7.47013723709267E-4 0.0 0.0 2.3714721387595777E-5 1.0078756589728205E-4 16-17 8.53729969953448E-4 0.0 0.0 2.3714721387595777E-5 1.5414568901937255E-4 18-19 9.485888555038312E-4 5.928680346898944E-6 0.0 2.3714721387595777E-5 1.7786041040696835E-4 20-21 0.0010375190607073152 1.1857360693797889E-5 0.0 2.3714721387595777E-5 1.8971777110076622E-4 22-23 0.0010849485034825066 1.1857360693797889E-5 0.0 3.557208208139367E-5 2.0750381214146307E-4 24-25 0.001167950028339092 1.1857360693797889E-5 0.0 4.150076242829261E-5 2.1936117283526094E-4 26-27 0.0012983809959708687 1.1857360693797889E-5 0.0 4.7429442775191555E-5 2.4900457456975564E-4 28-29 0.0013576677994398584 2.3714721387595777E-5 0.0 4.7429442775191555E-5 2.7271929595735144E-4 30-31 0.0014406693242964433 2.3714721387595777E-5 0.0 5.33581231220905E-5 3.14220058385644E-4 32-33 0.0015711002919282203 2.3714721387595777E-5 0.0 7.707284450968627E-5 3.4979214046703777E-4 34-35 0.0017548893826820876 2.3714721387595777E-5 0.0 8.300152485658522E-5 3.557208208139367E-4 36-37 0.0018912490306607632 2.3714721387595777E-5 0.0 8.300152485658522E-5 3.6757818150773454E-4 38-39 0.002039466039333237 2.3714721387595777E-5 0.0 9.485888555038311E-5 3.6757818150773454E-4 40-41 0.002235112490780902 2.3714721387595777E-5 0.0 9.485888555038311E-5 3.6757818150773454E-4 42-43 0.0023892581798002745 2.3714721387595777E-5 0.0 9.485888555038311E-5 3.7943554220153244E-4 44-45 0.0025374751884727482 2.3714721387595777E-5 0.0 9.485888555038311E-5 3.9722158324222926E-4 46-47 0.0027034782381859185 2.3714721387595777E-5 0.0 9.485888555038311E-5 4.0315026358912824E-4 48-49 0.00286355260755219 2.3714721387595777E-5 0.0 9.485888555038311E-5 4.0315026358912824E-4 50-51 0.0030176982965715625 2.3714721387595777E-5 0.0 9.485888555038311E-5 4.150076242829261E-4 52-53 0.0031422005838564406 2.3714721387595777E-5 0.0 9.485888555038311E-5 4.26864984976724E-4 54-55 0.003302274953222712 2.3714721387595777E-5 0.0 9.485888555038311E-5 4.327936653236229E-4 56-57 0.003408991199466893 2.3714721387595777E-5 0.0 9.485888555038311E-5 4.4465102601742087E-4 58-59 0.003610566331261457 2.3714721387595777E-5 0.0 1.0078756589728205E-4 4.624370670581177E-4 60-61 0.0038536422254843135 2.3714721387595777E-5 0.0 1.06716246244181E-4 4.683657474050166E-4 62-63 0.004055217357278878 2.3714721387595777E-5 0.0 1.1857360693797888E-4 4.8615178844571344E-4 64-65 0.004298293251501734 2.3714721387595777E-5 0.0 1.1857360693797888E-4 4.8615178844571344E-4 66-67 0.004523583104683895 2.3714721387595777E-5 0.0 1.1857360693797888E-4 4.8615178844571344E-4 68-69 0.004707372195437762 2.3714721387595777E-5 0.0 1.1857360693797888E-4 5.276525508740061E-4 70-71 0.004980091491395113 2.3714721387595777E-5 0.0 1.1857360693797888E-4 5.632246329553997E-4 72-73 0.005199452664230374 2.3714721387595777E-5 0.0 1.1857360693797888E-4 5.691533133022987E-4 74-75 0.005383241754984242 2.3714721387595777E-5 0.0 1.1857360693797888E-4 5.928680346898945E-4 76-77 0.005650032370594694 2.3714721387595777E-5 0.0 1.1857360693797888E-4 5.987967150367934E-4 78-79 0.005964252428980338 2.3714721387595777E-5 0.0 1.1857360693797888E-4 6.40297477465086E-4 80-81 0.006314044569447376 2.3714721387595777E-5 0.0 1.1857360693797888E-4 6.40297477465086E-4 82-83 0.006865411841708977 2.3714721387595777E-5 0.0 1.2450228728487782E-4 6.699408791995807E-4 84-85 0.0075887108440306494 2.3714721387595777E-5 0.0 1.3635964797867572E-4 7.232990023216712E-4 86-87 0.008495798937106187 2.3714721387595777E-5 0.0 1.4228832832557467E-4 7.41085043362368E-4 88-89 0.009847538056199147 3.557208208139367E-5 0.0 1.4228832832557467E-4 7.58871084403065E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTCACG 380 0.0 24.99928 3 CGTACGC 230 0.0 21.683645 20-21 CAACGCA 8655 0.0 20.469631 12-13 CTCGCGT 245 0.0 20.358187 52-53 TCAACGC 8500 0.0 20.340048 10-11 ACTCGCG 235 0.0 20.21446 50-51 ATCAACG 8880 0.0 20.031265 10-11 ACGTACG 275 0.0 18.999002 20-21 AACGCAG 9185 0.0 18.926493 12-13 CGCGTAG 265 0.0 18.820772 54-55 TCGCGTA 305 0.0 17.910755 52-53 GGTATCA 10000 0.0 17.859274 8 ACGCAGA 10185 0.0 17.394665 14-15 GTATCAA 10685 0.0 16.714243 9 AAGCAGT 11615 0.0 16.585169 1 AGTGGTA 10890 0.0 16.530647 5 CGCAGAG 10540 0.0 16.515875 14-15 TATCAAC 11120 0.0 15.932261 9 TGGTATC 11680 0.0 15.209144 7 CGTAGCG 315 0.0 15.078842 56-57 >>END_MODULE