FastQCFastQC Report
Sat 18 Jun 2016
SRR578600_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR578600_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8378549
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1385251.6533292339759544No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT132730.15841645134497634No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT114540.13670624830146605No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCACG3700.026.946383
CGTACGC2050.025.47483320-21
TCAACGC82550.020.3014110-11
ATCAACG86750.020.2489349
ACGTACG2350.020.20247720-21
CGCGTAG2600.019.17401554-55
AACGCAG87300.019.06079912-13
CTCGCGT2500.018.99316252-53
CAACGCA84900.018.64899412-13
GGTATCA96800.018.4900676
AGTGGTA102450.017.285413
TCGCGTA2900.017.19208752-53
GTATCAA103800.016.9229817
TATCAAC108050.016.5648128
CGCAGAG102100.016.27457414-15
TGGTATC111500.016.1377145
ACGCAGA98450.016.05816314-15
ACTCGCG3000.015.82782550-51
AAGCAGT112100.015.1041981
GTGGTAT123100.014.5401464
Produced by FastQC (version 0.11.5)