FastQCFastQC Report
Sat 18 Jun 2016
SRR578599_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR578599_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8477944
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1104911.3032758885880822No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT143810.16962839103443003No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113040.13333421405001022No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT86570.10211202149955224No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC92950.020.2996510-11
CAACGCA94300.019.75767112-13
CGTCACG2651.895387E-819.6835823
ATCAACG96750.019.307839
AACGCAG98950.019.0447912-13
GGTATCA108450.017.7509067
AGTGGTA112400.017.0008434
ACGCAGA110550.016.87488714-15
GTATCAA115600.016.817068
CGCAGAG114550.016.47186914-15
TATCAAC118300.016.3918049
AAGCAGT118600.016.2748991
CGTACGC1752.5635018E-816.25669120-21
TGGTATC122800.015.5607576
CGCGTAG1754.5417437E-714.89976954-55
AGAGTAC120500.014.87271918-19
GTGGTAT132300.014.6226795
CAGAGTA130800.014.280498516-17
GAGTACT78300.013.20019618-19
CTCGCGT2002.0466287E-613.03729752-53
Produced by FastQC (version 0.11.5)