##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR578599_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8477944
Sequences flagged as poor quality	0
Sequence length	101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.998812801783075	31.0	31.0	34.0	30.0	34.0
2	32.26968177661943	34.0	31.0	34.0	30.0	34.0
3	32.343313190084764	34.0	31.0	34.0	30.0	34.0
4	35.90900423498905	37.0	35.0	37.0	35.0	37.0
5	35.78543241144315	37.0	35.0	37.0	33.0	37.0
6	35.76073857057796	37.0	35.0	37.0	33.0	37.0
7	35.7547657781179	37.0	35.0	37.0	33.0	37.0
8	35.672059169062685	37.0	35.0	37.0	33.0	37.0
9	37.31085001269176	39.0	37.0	39.0	34.0	39.0
10-11	37.30880128483982	39.0	37.0	39.0	33.5	39.0
12-13	37.30493861483397	39.0	37.0	39.0	33.5	39.0
14-15	38.621364743621804	40.0	38.0	41.0	34.0	41.0
16-17	38.575901716265165	40.0	38.0	41.0	34.0	41.0
18-19	38.517390949975606	40.0	38.0	41.0	34.0	41.0
20-21	38.44898586261009	40.0	38.0	41.0	34.0	41.0
22-23	38.33059465832754	40.0	38.0	41.0	33.5	41.0
24-25	38.25367801438651	40.0	38.0	41.0	33.0	41.0
26-27	38.09164586366695	40.0	37.0	41.0	33.0	41.0
28-29	37.99937343299272	40.0	37.0	41.0	33.0	41.0
30-31	37.904306338895374	40.0	37.0	41.0	33.0	41.0
32-33	37.77374172322912	40.0	37.0	41.0	32.5	41.0
34-35	37.646508162828155	40.0	37.0	41.0	32.0	41.0
36-37	37.52121911869199	40.0	37.0	41.0	32.0	41.0
38-39	37.39043298705441	40.0	36.0	41.0	31.0	41.0
40-41	37.24569842641093	39.5	36.0	41.0	31.0	41.0
42-43	37.08861098870197	39.0	36.0	41.0	31.0	41.0
44-45	36.936344767080314	39.0	35.5	41.0	30.5	41.0
46-47	37.01776834100343	39.0	36.0	41.0	31.0	41.0
48-49	36.99860797617913	39.0	36.0	41.0	31.0	41.0
50-51	36.972184942481334	39.0	36.0	41.0	31.0	41.0
52-53	36.979821935601365	39.0	36.0	41.0	31.0	41.0
54-55	36.84738740902275	39.0	35.0	41.0	31.0	41.0
56-57	36.69225699060999	39.0	35.0	41.0	30.5	41.0
58-59	36.47482502833234	39.0	35.0	41.0	30.0	41.0
60-61	36.24566427898085	38.5	35.0	40.5	30.0	41.0
62-63	35.97254977150121	38.0	35.0	40.0	29.0	41.0
64-65	35.65832382237957	37.5	34.5	40.0	29.0	41.0
66-67	35.335764248973575	37.0	34.0	40.0	29.0	41.0
68-69	35.00054441265476	36.5	34.0	39.0	28.5	41.0
70-71	34.63063143611234	36.0	34.0	39.0	28.0	41.0
72-73	34.20613252458379	35.5	33.5	39.0	28.0	40.5
74-75	33.81871365274411	35.0	33.0	37.5	27.0	40.0
76-77	32.39073028790943	34.5	31.5	36.0	26.0	39.0
78-79	32.984829635581455	35.0	32.5	36.5	26.0	39.0
80-81	32.93044929289459	35.0	33.0	36.0	26.0	39.0
82-83	32.65752681310468	35.0	33.0	36.0	26.0	37.0
84-85	32.37699853879667	35.0	33.0	35.5	26.0	37.0
86-87	32.100294953587806	35.0	32.5	35.0	26.0	37.0
88-89	31.855950511114486	35.0	32.0	35.0	25.0	36.0
90-91	31.6213557791842	35.0	32.0	35.0	25.0	36.0
92-93	31.38515145889145	35.0	32.0	35.0	24.5	36.0
94-95	31.14023618226306	35.0	32.0	35.0	24.0	35.5
96-97	30.544421147391397	34.0	31.5	35.0	19.0	35.0
98-99	30.51897618101747	34.0	31.5	35.0	19.5	35.0
100-101	29.9431773198785	34.0	30.5	35.0	10.0	35.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.4481543438800202
1101	2	0.42281016292309914
1101	3	0.3969807918508579
1101	4	0.299605486563415
1101	5	0.28102130819265625
1101	6	0.30729902896555217
1101	7	0.31887791837173296
1101	8	0.3884717670421267
1101	9	0.46164766143581915
1101	10-11	0.470300909481594
1101	12-13	0.47682717071241854
1101	14-15	0.6137943416141738
1101	16-17	0.598053356293768
1101	18-19	0.5849116157781538
1101	20-21	0.6282705537115518
1101	22-23	0.6872979469137874
1101	24-25	0.7341196746893175
1101	26-27	0.7340863031085618
1101	28-29	0.7267355305256231
1101	30-31	0.6739941731779169
1101	32-33	0.6706664591733542
1101	34-35	0.7042332164652407
1101	36-37	0.6870993542321884
1101	38-39	0.7307602907489397
1101	40-41	0.7517398914204634
1101	42-43	0.7974219128050422
1101	44-45	0.753021788819602
1101	46-47	0.7761429955080601
1101	48-49	0.717821670985785
1101	50-51	0.6981162180438218
1101	52-53	0.698226133943173
1101	54-55	0.7379440622946731
1101	56-57	0.7266206954945886
1101	58-59	0.7482308091974517
1101	60-61	0.7115028104703853
1101	62-63	0.7310150807726927
1101	64-65	0.6752109548616758
1101	66-67	0.6901348071133029
1101	68-69	0.6862777040051427
1101	70-71	0.6623518000893824
1101	72-73	0.6538155237788672
1101	74-75	0.6939730392145691
1101	76-77	0.6589093839197986
1101	78-79	0.5427643194951344
1101	80-81	0.47387848948709177
1101	82-83	0.5066680060489901
1101	84-85	0.4516414815553489
1101	86-87	0.4717592214656037
1101	88-89	0.5359322792303978
1101	90-91	0.5374842884567919
1101	92-93	0.5998278497587641
1101	94-95	0.6021122839552966
1101	96-97	0.9507637489829683
1101	98-99	0.7170261823952444
1101	100-101	0.6610405646358082
1102	1	0.39028506227159454
1102	2	0.34667508194021224
1102	3	0.3377245053005353
1102	4	0.2449833596700799
1102	5	0.3062755885435706
1102	6	0.31237521624878184
1102	7	0.260229044330444
1102	8	0.3187379063436211
1102	9	0.4157042601030838
1102	10-11	0.38938213419412904
1102	12-13	0.4052611715579175
1102	14-15	0.48026621516038404
1102	16-17	0.5158971836201758
1102	18-19	0.5106142142718326
1102	20-21	0.5721326952191816
1102	22-23	0.5529106560552393
1102	24-25	0.6139401147093437
1102	26-27	0.6423227152429121
1102	28-29	0.657690170797899
1102	30-31	0.6663805630081896
1102	32-33	0.6992126161081273
1102	34-35	0.7017967422248006
1102	36-37	0.7442175067555965
1102	38-39	0.7624519392527205
1102	40-41	0.7522060516495159
1102	42-43	0.7445540001203241
1102	44-45	0.7919422819401021
1102	46-47	0.8015528764659337
1102	48-49	0.8125524607923609
1102	50-51	0.8069400104396252
1102	52-53	0.7193841565010786
1102	54-55	0.7452480606484642
1102	56-57	0.6804115438983303
1102	58-59	0.6929933519682976
1102	60-61	0.7306913064250864
1102	62-63	0.7006938433123153
1102	64-65	0.6848876473926566
1102	66-67	0.7234828562307669
1102	68-69	0.7705167754794076
1102	70-71	0.7416944612350918
1102	72-73	0.765768476314598
1102	74-75	0.6334564912773928
1102	76-77	0.5943885008047616
1102	78-79	0.6388001654654971
1102	80-81	0.655120708727658
1102	82-83	0.5950116614287708
1102	84-85	0.6127406328109757
1102	86-87	0.5994131661823161
1102	88-89	0.5078707428722034
1102	90-91	0.5377944689564167
1102	92-93	0.5373945753118967
1102	94-95	0.6054429039383393
1102	96-97	1.0154709707875718
1102	98-99	0.8204732017227911
1102	100-101	0.8143070021025309
1103	1	0.33911162674542794
1103	2	0.29380430964626925
1103	3	0.2843601779911964
1103	4	0.2522569632881755
1103	5	0.2435396054552328
1103	6	0.30741410981285355
1103	7	0.2745410155471433
1103	8	0.09957642191697147
1103	9	0.19483638341893794
1103	10-11	0.37089596844383266
1103	12-13	0.36885824975865233
1103	14-15	0.4760177289829599
1103	16-17	0.45735221499386824
1103	18-19	0.4140298677487593
1103	20-21	0.38460275806754396
1103	22-23	0.44486843139100785
1103	24-25	0.25655741401807575
1103	26-27	0.29649726916738217
1103	28-29	0.48583316663444975
1103	30-31	0.2961677021355058
1103	32-33	0.5412594224657354
1103	34-35	0.5239964301270064
1103	36-37	0.611256407601779
1103	38-39	0.6242330403521166
1103	40-41	0.47386893469056446
1103	42-43	0.5964056279992747
1103	44-45	0.6755870741943326
1103	46-47	0.5680226889229303
1103	48-49	0.5772559631700105
1103	50-51	0.5737511644500586
1103	52-53	0.5834090534621552
1103	54-55	0.6562035806922211
1103	56-57	0.570656003896957
1103	58-59	0.3417213987340588
1103	60-61	0.2949967397015101
1103	62-63	0.17404611372848677
1103	64-65	0.4114835184316945
1103	66-67	0.37347823818486603
1103	68-69	0.34484242726723124
1103	70-71	0.3490973019592616
1103	72-73	0.5284719738761936
1103	74-75	0.4463641370032505
1103	76-77	0.29100353662548883
1103	78-79	0.4480224245221649
1103	80-81	0.43953821531246007
1103	82-83	0.4506842816634773
1103	84-85	0.47854346656346536
1103	86-87	0.3724252725851116
1103	88-89	0.30806661344123043
1103	90-91	0.42486227803010834
1103	92-93	0.44991558172705837
1103	94-95	0.4477291803099419
1103	96-97	0.5739655721055108
1103	98-99	0.2978704981082352
1103	100-101	0.5920855890034105
1104	1	0.34286046986368035
1104	2	0.3282352342293322
1104	3	0.2922608176629282
1104	4	0.19415948273403671
1104	5	0.21432170035627252
1104	6	0.2383456259925083
1104	7	0.24625737821219928
1104	8	0.3241026822896913
1104	9	0.2957899914628186
1104	10-11	0.3521217946484114
1104	12-13	0.37151224243429937
1104	14-15	0.45820486337117217
1104	16-17	0.4545047690575643
1104	18-19	0.45003061268312194
1104	20-21	0.4211355904805316
1104	22-23	0.5583673325432486
1104	24-25	0.5414416197648464
1104	26-27	0.5973656934014926
1104	28-29	0.6194718710814513
1104	30-31	0.6521326483945842
1104	32-33	0.651800003671049
1104	34-35	0.6419654655160514
1104	36-37	0.7192362174554319
1104	38-39	0.6978881859653754
1104	40-41	0.6536539444673721
1104	42-43	0.7402208476702654
1104	44-45	0.6935089124809224
1104	46-47	0.5264122770162132
1104	48-49	0.65206609189989
1104	50-51	0.5568042086233334
1104	52-53	0.6193432552471165
1104	54-55	0.6658750470549961
1104	56-57	0.6893349674449567
1104	58-59	0.7354507690376195
1104	60-61	0.6587831183247772
1104	62-63	0.4601978941124969
1104	64-65	0.6585887130134438
1104	66-67	0.7126742693433172
1104	68-69	0.7045942595809507
1104	70-71	0.6593141168520873
1104	72-73	0.7872546360277113
1104	74-75	0.7092079443414789
1104	76-77	0.6398832171136917
1104	78-79	0.6690790804261155
1104	80-81	0.7317877995533877
1104	82-83	0.7762217956987172
1104	84-85	0.8242825890221255
1104	86-87	0.7987020537427227
1104	88-89	0.7674807187715551
1104	90-91	0.7979739194991673
1104	92-93	0.8272102791523146
1104	94-95	0.8965329937141355
1104	96-97	1.217601402994294
1104	98-99	0.9822837287339681
1104	100-101	1.0137875903653537
1105	1	0.15600884386525848
1105	2	0.15737613253693894
1105	3	0.13244235730056886
1105	4	0.06112912951742544
1105	5	0.08910751100868453
1105	6	0.06203093756397493
1105	7	0.10969840023143718
1105	8	0.17559035088742547
1105	9	0.15126812387726574
1105	10-11	0.13950172684609186
1105	12-13	0.13862712321083137
1105	14-15	0.152408031311019
1105	16-17	0.14119201798963132
1105	18-19	0.05748904607077776
1105	20-21	0.16508160624407253
1105	22-23	0.17730347062016705
1105	24-25	0.17525202737843415
1105	26-27	0.13444541451067238
1105	28-29	0.14369748392169868
1105	30-31	0.12141887384059658
1105	32-33	0.13973256588186445
1105	34-35	0.14910932696415813
1105	36-37	0.1716246888579036
1105	38-39	0.19138497680047095
1105	40-41	0.22633283806028714
1105	42-43	0.1655380527026864
1105	44-45	0.2040626823920988
1105	46-47	0.20328915517401214
1105	48-49	0.2167690764908201
1105	50-51	0.13206120966655988
1105	52-53	0.1912615272865068
1105	54-55	0.24633911125932428
1105	56-57	0.23512903040935385
1105	58-59	0.23949900452969075
1105	60-61	0.23805720118186713
1105	62-63	0.26981374428531524
1105	64-65	0.2360417553912555
1105	66-67	0.24605982177330077
1105	68-69	0.21113442458342746
1105	70-71	0.24077577083671997
1105	72-73	0.23481423833838733
1105	74-75	0.24176400750379656
1105	76-77	0.2895383155511766
1105	78-79	0.2757571166233319
1105	80-81	0.24221605639646526
1105	82-83	0.21531449224647758
1105	84-85	0.266522168436758
1105	86-87	0.13923930323777967
1105	88-89	0.1575833385721488
1105	90-91	0.2095078923007172
1105	92-93	0.16631461142748805
1105	94-95	0.2264398682456239
1105	96-97	0.6206712731716202
1105	98-99	0.40276119322463444
1105	100-101	0.394526470572508
1106	1	-0.057477719807558
1106	2	-0.08674030053077075
1106	3	-0.07799591146479656
1106	4	-0.04392926237962769
1106	5	-0.04588521649031918
1106	6	-0.06797126593604474
1106	7	-0.06389576090559501
1106	8	-0.037054179280382016
1106	9	0.006011260683045805
1106	10-11	-0.04863604340207672
1106	12-13	-0.0764997794444966
1106	14-15	-0.15310466147817436
1106	16-17	-0.09510088972967168
1106	18-19	-0.13842707375221863
1106	20-21	-0.14769185622763814
1106	22-23	-0.11239953624909305
1106	24-25	0.006716019793735484
1106	26-27	-0.08564860437540034
1106	28-29	-0.05852698067802464
1106	30-31	-0.18678102464771484
1106	32-33	-0.20778039592597253
1106	34-35	-0.24942808008810857
1106	36-37	-0.20688298350141565
1106	38-39	-0.2691268618489957
1106	40-41	-0.2402492901915565
1106	42-43	-0.18616195536324653
1106	44-45	-0.16744052925123754
1106	46-47	-0.25465842037370123
1106	48-49	-0.19204353313251232
1106	50-51	-0.17151234763588263
1106	52-53	-0.09565731451856863
1106	54-55	-0.1111705250605155
1106	56-57	-0.1208379334026759
1106	58-59	-0.06822894702286675
1106	60-61	-0.16706449105054588
1106	62-63	-0.08987268037461149
1106	64-65	-0.05460032054520525
1106	66-67	-0.04303084031689508
1106	68-69	-0.03973127576017532
1106	70-71	-0.07847283504781899
1106	72-73	-0.015227255588300181
1106	74-75	0.02256618001778321
1106	76-77	-0.06749989806937862
1106	78-79	-0.05685407893184191
1106	80-81	-0.10765310454184629
1106	82-83	-0.09520883144887193
1106	84-85	-0.126510706847192
1106	86-87	-0.16582485952754666
1106	88-89	-0.24539910613809468
1106	90-91	-0.1616985435871392
1106	92-93	-0.10178997757769892
1106	94-95	-0.04721120973330528
1106	96-97	0.22166003254185895
1106	98-99	0.018876149969273115
1106	100-101	0.07057452474569104
1107	1	-0.21242642543061052
1107	2	-0.24579360882389523
1107	3	-0.1977561404530661
1107	4	-0.13588737858764688
1107	5	-0.14538269792060987
1107	6	-0.1085817611844746
1107	7	-0.18085164334389248
1107	8	-0.15896481534664986
1107	9	-0.17674026353729033
1107	10-11	-0.2572739363103693
1107	12-13	-0.18624401173929783
1107	14-15	-0.23658005413321348
1107	16-17	-0.28043489256527465
1107	18-19	-0.3056521790832747
1107	20-21	-0.31043947716690923
1107	22-23	-0.20222768584010709
1107	24-25	-0.13730383904015042
1107	26-27	-0.15851910896184762
1107	28-29	-0.17321820402516153
1107	30-31	-0.22575608538807046
1107	32-33	-0.34086294804784956
1107	34-35	-0.2994351484221056
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2308	24-25	-1.1718111324926923
2308	26-27	-1.1743077458881501
2308	28-29	-1.2184529108884234
2308	30-31	-1.091724781547157
2308	32-33	-1.1661915547385107
2308	34-35	-1.2525219090273083
2308	36-37	-1.2461163392473154
2308	38-39	-1.257690361754868
2308	40-41	-1.2318279228063957
2308	42-43	-1.1527671340869716
2308	44-45	-1.1395738871184022
2308	46-47	-1.1527470325592688
2308	48-49	-1.2552826041285812
2308	50-51	-1.2652499338160794
2308	52-53	-1.2683911046512648
2308	54-55	-1.2008556893551727
2308	56-57	-1.0579183493830158
2308	58-59	-1.051491927044303
2308	60-61	-1.004242968202064
2308	62-63	-1.0724022577082408
2308	64-65	-1.0239358711139985
2308	66-67	-1.0056293593345629
2308	68-69	-1.0674747543743237
2308	70-71	-1.0556361283239042
2308	72-73	-1.1180792483183026
2308	74-75	-1.1798949601056279
2308	76-77	-1.1370608939424969
2308	78-79	-1.5912774172703656
2308	80-81	-1.264337641603639
2308	82-83	-1.1330471345122035
2308	84-85	-1.085124113033281
2308	86-87	-1.0272374274511833
2308	88-89	-1.066374887035476
2308	90-91	-1.1427715960249571
2308	92-93	-1.0453536685993718
2308	94-95	-1.1379607716500395
2308	96-97	-1.0097457450967795
2308	98-99	-1.3294752200445394
2308	100-101	-1.3407365652921222
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	46.0
3	73.0
4	167.0
5	463.0
6	881.0
7	1652.0
8	3055.0
9	4723.0
10	6583.0
11	8320.0
12	9999.0
13	11754.0
14	13139.0
15	14641.0
16	17330.0
17	20583.0
18	24673.0
19	28997.0
20	31287.0
21	30986.0
22	32928.0
23	38049.0
24	46950.0
25	59198.0
26	76168.0
27	96615.0
28	123096.0
29	155249.0
30	192293.0
31	237363.0
32	292673.0
33	368534.0
34	485355.0
35	722312.0
36	994220.0
37	1718611.0
38	2039244.0
39	567404.0
40	2330.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	17.955088831758243	30.229010317137217	28.699140850033388	23.116760001071153
2	22.62000250813226	29.52839681153392	27.24074660231251	20.61085407802132
3	22.002853217820554	28.171715868198984	27.671470865163872	22.15396004881659
4	22.1092236780226	28.046752687314658	27.91199033438403	21.932033300278714
5	22.603174782914248	28.18231133736288	28.26730909210579	20.94720478761708
6	22.60009030491355	28.225499012496424	27.21343759760621	21.960973084983813
7	22.800174193177025	28.213444202981293	27.855810323823793	21.13057128001789
8	21.965610915667202	28.46860249917338	27.75885858712043	21.806927998038994
9	21.514610375439215	28.21623964351783	28.407461645301947	21.861688335741007
10-11	21.583758986848697	28.656157672190336	27.762356061799885	21.99772727916108
12-13	21.55327989899438	28.834243302385577	27.539395164676716	22.07308163394332
14-15	21.76193897954504	28.7922107058032	27.390190357473465	22.055659957178296
16-17	21.965531973318058	28.55025345767795	27.36314960325286	22.12106496575113
18-19	21.93235175886984	28.677070761495948	27.360666689942747	22.029910789691463
20-21	22.030618508449688	28.527559276164126	27.48459414216466	21.957228073221525
22-23	21.89143605033962	28.49402342599034	27.552715083651275	22.061825440018758
24-25	21.877637303758355	28.38160989529198	27.559784095042346	22.18096870590732
26-27	22.1426145211092	28.22482703731934	27.718311693374847	21.914246748196618
28-29	22.106839818710764	28.331733495762652	27.6233718929967	21.938054792529886
30-31	22.06756142762915	28.309387275971627	27.623295223464556	21.999756072934666
32-33	22.05716975719585	28.35543617650694	27.547056220234527	22.040337846062677
34-35	22.054026306378056	28.40014630905795	27.502912262690103	22.04291512187389
36-37	22.05216264698139	28.406073453658102	27.493369854766676	22.04839404459383
38-39	22.048883550068272	28.38438187371844	27.50875094244548	22.05798363376781
40-41	22.059015723623556	28.4521164565371	27.465975241166962	22.022892578672376
42-43	22.10308890929216	28.407252984921815	27.42929181886552	22.06036628692051
44-45	22.084222306729085	28.439489574359065	27.433154783754176	22.043133335157673
46-47	22.102245544438603	28.469526337989493	27.401230770101808	22.026997347470097
48-49	22.120091852458568	28.443818454096892	27.378141445614645	22.0579482478299
50-51	22.14371786367072	28.43059590863068	27.36997319161344	22.055713036085166
52-53	22.10012828582024	28.501173161794892	27.319560025402385	22.079138526982486
54-55	22.163327570929933	28.49575911329445	27.21283603666172	22.128077279113896
56-57	22.136646573744766	28.526037680833937	27.226359362600178	22.11095638282112
58-59	22.146041540260235	28.61082828572588	27.14990804374268	22.093222130271208
60-61	22.131169584690433	28.600358270952817	27.149610972758598	22.11886117159815
62-63	22.120826968034134	28.62354959495731	27.17475314001932	22.080870296989236
64-65	22.100542721659806	28.673907398378507	27.15588148199349	22.069668397968204
66-67	22.125060082508398	28.641094371559078	27.160625978644536	22.073219567287985
68-69	22.0686533065052	28.720010394063213	27.18102967869523	22.030306620736358
70-71	22.116998079956744	28.727258315528285	27.135184114431652	22.020559490083315
72-73	22.072350935660502	28.7173130411433	27.143717611167755	22.06661841202844
74-75	22.155433186971102	28.70271469886223	27.1094087318515	22.03244338231517
76-77	22.104950563220672	28.803715504398408	27.081838494651706	22.009495437729214
78-79	22.14830961547086	28.766094825815923	27.052791028676232	22.032804530036987
80-81	22.10176578314208	28.806243923935277	27.046836152847543	22.0451541400751
82-83	22.119409807156686	28.863078150272997	27.01635902267557	22.001153019894755
84-85	22.103334050759692	28.88130856208942	26.996421695940477	22.018935691210416
86-87	22.07868868217587	28.915658880179766	26.98699606911457	22.018656368529793
88-89	22.061783974831727	28.960074798040097	26.971710494922835	22.00643073220535
90-91	22.058175997266993	28.953167414386932	26.976331947043015	22.012324641303064
92-93	22.053387343274583	28.970119313428626	26.972942975585195	22.003550367711593
94-95	22.05326875712728	29.049066399192004	26.942608947844683	21.955055895836033
96-97	22.027941178226346	29.03380091000154	26.940701854507203	21.99755605726491
98-99	22.033207354024913	29.098416726111097	26.886269333507407	21.98210658635658
100-101	22.082741052462108	29.142178215764964	26.812833893853433	21.962246837919494
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	120480.0
1	67625.5
2	10701.0
3	6066.0
4	5161.0
5	4384.0
6	4070.5
7	4171.5
8	3984.0
9	3725.0
10	3602.5
11	4449.0
12	9681.0
13	9764.5
14	5493.5
15	5687.0
16	6301.0
17	7459.0
18	9151.0
19	10587.5
20	12703.5
21	16355.0
22	20496.5
23	25575.5
24	31477.5
25	39225.5
26	50414.0
27	62838.0
28	73454.5
29	83836.0
30	98220.0
31	115517.0
32	132787.5
33	150292.5
34	168126.0
35	185443.0
36	204188.5
37	223695.5
38	237219.0
39	242727.5
40	251593.5
41	263367.0
42	270428.5
43	283186.5
44	298015.5
45	307709.0
46	313992.0
47	317168.0
48	324985.0
49	331866.0
50	330478.5
51	324285.5
52	316789.0
53	307533.5
54	290492.5
55	267573.5
56	241672.5
57	216502.5
58	196600.5
59	169471.5
60	134936.0
61	102657.5
62	72781.5
63	49448.0
64	32652.5
65	19836.0
66	11324.0
67	6657.5
68	3628.5
69	1702.0
70	798.5
71	373.5
72	187.0
73	109.5
74	71.5
75	52.5
76	39.5
77	25.5
78	15.5
79	8.5
80	6.0
81	4.5
82	5.0
83	4.5
84	1.5
85	1.0
86	2.0
87	2.5
88	2.0
89	0.5
90	0.5
91	1.0
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.012703551710178788
2	0.017893489270511813
3	0.005874065693286014
4	6.72332820315869E-4
5	8.610578225097973E-4
6	0.0
7	0.0
8	0.007796701653136656
9	0.010450646996488771
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	1.179531263712051E-4
24-25	0.004924543025997812
26-27	0.0071892430523249505
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	3.5385937911361526E-5
62-63	1.2385078268976535E-4
64-65	3.0078047224657296E-4
66-67	2.241109401052897E-4
68-69	3.24371097520814E-4
70-71	1.179531263712051E-4
72-73	2.9488281592801275E-5
74-75	6.48742195041628E-5
76-77	0.12520134598671565
78-79	0.05572105689775729
80-81	7.077187582272305E-5
82-83	0.002435732059565385
84-85	0.01042115871489597
86-87	0.11800620527807214
88-89	0.13460810781481927
90-91	0.14797809468899537
92-93	0.14557774856734132
94-95	0.11327038725426825
96-97	1.2047024608796661
98-99	0.07604438057151593
100-101	0.0678584336013543
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	8477944.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.565989419026415
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.05405637520747	56.34321138311639
2	5.36001869295696	6.707097456586579
3	1.4295740747223145	2.6832814929837236
4	0.7263421926406163	1.8177727175738099
5	0.4330067137273912	1.3545746734717679
6	0.2752424664312511	1.0332490345442635
7	0.20006222356063688	0.8761963673709192
8	0.1505980720914666	0.7537853908000383
9	0.11332755728904244	0.6381405675207305
>10	1.1335382341286666	15.202271264530816
>50	0.11194867171425597	4.624056175878435
>100	0.011717557005319556	0.9336776031130901
>500	1.417921311592434E-4	0.06910793317714323
>1k	7.08960655796217E-5	0.17276983294285808
>5k	1.417921311592434E-4	0.750473120297873
>10k+	2.1268819673886507E-4	6.040334986091564
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	110491	1.3032758885880822	No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	14381	0.16962839103443003	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11304	0.13333421405001022	No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	8657	0.10211202149955224	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	7.077187582272305E-5	0.0	0.0	0.0	1.1795312637120509E-5
2	1.0615781373408458E-4	0.0	0.0	0.0	1.1795312637120509E-5
3	2.0052031483104867E-4	0.0	0.0	0.0	1.1795312637120509E-5
4	2.830875032908922E-4	0.0	0.0	0.0	3.5385937911361526E-5
5	3.1847344120225377E-4	0.0	0.0	0.0	3.5385937911361526E-5
6	3.656546917507358E-4	0.0	0.0	2.3590625274241017E-5	3.5385937911361526E-5
7	4.0104062966209733E-4	0.0	0.0	2.3590625274241017E-5	3.5385937911361526E-5
8	4.1283594229921783E-4	0.0	0.0	2.3590625274241017E-5	3.5385937911361526E-5
9	5.071984433961819E-4	0.0	0.0	2.3590625274241017E-5	3.5385937911361526E-5
10-11	5.307890686704229E-4	0.0	0.0	2.948828159280127E-5	4.1283594229921783E-5
12-13	6.369468824045075E-4	0.0	0.0	4.1283594229921783E-5	7.077187582272307E-5
14-15	7.077187582272305E-4	0.0	0.0	5.307890686704229E-5	1.2385078268976535E-4
16-17	8.07978915642755E-4	0.0	0.0	5.897656318560255E-5	1.415437516454461E-4
18-19	9.200343856953998E-4	0.0	0.0	5.897656318560255E-5	1.415437516454461E-4
20-21	0.0010026015741552434	0.0	0.0	5.897656318560255E-5	1.415437516454461E-4
22-23	0.0011028617315707676	0.0	0.0	5.897656318560255E-5	1.5923672060112688E-4
24-25	0.0012503031395347738	0.0	0.0	5.897656318560255E-5	1.6513437691968713E-4
26-27	0.0013918468911802203	0.0	0.0	5.897656318560255E-5	1.7103203323824738E-4
28-29	0.0014508234543658227	0.0	0.0	5.897656318560255E-5	1.8872500219392814E-4
30-31	0.0015156976738699853	0.0	0.0	5.897656318560255E-5	2.241109401052897E-4
32-33	0.0016277531439226303	0.0	0.0	5.897656318560255E-5	2.712921906537717E-4
34-35	0.0018341711150722394	0.0	0.0	6.48742195041628E-5	2.8898515960945246E-4
36-37	0.0020523843988589684	0.0	0.0	8.256718845984357E-5	3.125757848836935E-4
38-39	0.0022411094010528964	0.0	0.0	8.256718845984357E-5	3.1847344120225377E-4
40-41	0.002453425028521066	0.0	0.0	8.256718845984357E-5	3.361664101579345E-4
42-43	0.0026362523743964335	0.0	0.0	8.256718845984357E-5	3.656546917507358E-4
44-45	0.0027718984697233193	0.0	0.0	8.256718845984357E-5	3.774500043878563E-4
46-47	0.002878056283457404	0.0	0.0	8.256718845984357E-5	3.774500043878563E-4
48-49	0.0030137023787842904	0.0	0.0	8.846484477840382E-5	4.0104062966209733E-4
50-51	0.003190632068341098	0.0	0.0	9.436250109696407E-5	4.2463125493633834E-4
52-53	0.0033144828510308635	1.1795312637120509E-5	0.0	9.436250109696407E-5	4.4822188021057934E-4
54-55	0.003408845352127827	1.1795312637120509E-5	0.0	9.436250109696407E-5	4.718125054848204E-4
56-57	0.0035091055095433513	1.1795312637120509E-5	0.0	9.436250109696407E-5	4.954031307590614E-4
58-59	0.0037096258243744	1.1795312637120509E-5	0.0	9.436250109696407E-5	5.130960997147421E-4
60-61	0.003916043795524009	1.1795312637120509E-5	0.0	9.436250109696407E-5	5.543796939446639E-4
62-63	0.004069382859806576	1.1795312637120509E-5	0.0	9.436250109696407E-5	5.661750065817844E-4
64-65	0.0042993914562304255	1.1795312637120509E-5	0.0	9.436250109696407E-5	5.779703192189049E-4
66-67	0.004511707083698595	1.1795312637120509E-5	0.0	9.436250109696407E-5	5.779703192189049E-4
68-69	0.004706329742211083	1.1795312637120509E-5	0.0	9.436250109696407E-5	6.192539134488267E-4
70-71	0.004859668806493651	1.1795312637120509E-5	0.0	9.436250109696407E-5	6.72332820315869E-4
72-73	0.005119165684510301	1.1795312637120509E-5	0.0	1.0026015741552432E-4	6.841281329529895E-4
74-75	0.005284300061429988	1.1795312637120509E-5	0.0	1.0615781373408458E-4	7.136164145457907E-4
76-77	0.005502513345216718	1.1795312637120509E-5	0.0	1.0615781373408458E-4	7.19514070864351E-4
78-79	0.00579739616114473	1.1795312637120509E-5	0.0	1.1205547005264485E-4	7.19514070864351E-4
80-81	0.0060745860081170625	1.1795312637120509E-5	0.0	1.179531263712051E-4	7.313093835014715E-4
82-83	0.006534603200964762	1.1795312637120509E-5	0.0	1.179531263712051E-4	7.43104696138592E-4
84-85	0.007295400866059036	1.1795312637120509E-5	0.0	1.179531263712051E-4	7.607976650942729E-4
86-87	0.008392364941311244	1.1795312637120509E-5	0.0	1.179531263712051E-4	7.666953214128332E-4
88-89	0.009825495426721384	1.1795312637120509E-5	0.0	1.179531263712051E-4	7.84388290368514E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCAACGC	9295	0.0	20.29965	10-11
CAACGCA	9430	0.0	19.757671	12-13
CGTCACG	265	1.895387E-8	19.683582	3
ATCAACG	9675	0.0	19.30783	9
AACGCAG	9895	0.0	19.04479	12-13
GGTATCA	10845	0.0	17.750906	7
AGTGGTA	11240	0.0	17.000843	4
ACGCAGA	11055	0.0	16.874887	14-15
GTATCAA	11560	0.0	16.81706	8
CGCAGAG	11455	0.0	16.471869	14-15
TATCAAC	11830	0.0	16.391804	9
AAGCAGT	11860	0.0	16.274899	1
CGTACGC	175	2.5635018E-8	16.256691	20-21
TGGTATC	12280	0.0	15.560757	6
CGCGTAG	175	4.5417437E-7	14.899769	54-55
AGAGTAC	12050	0.0	14.872719	18-19
GTGGTAT	13230	0.0	14.622679	5
CAGAGTA	13080	0.0	14.2804985	16-17
GAGTACT	7830	0.0	13.200196	18-19
CTCGCGT	200	2.0466287E-6	13.037297	52-53
>>END_MODULE