FastQCFastQC Report
Sat 18 Jun 2016
SRR578598_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR578598_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8983334
Sequences flagged as poor quality0
Sequence length101
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT106435511.84810672741323No Hit
AAGCAGTGGTATCAACGCAGAGTATTTTTTTTTTTTTTTTTTTTTTTTTT248590.2767235416160637No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGACTGCGCCTCAGTGCCT159620.17768458792693226No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTCAGGCAGCGAGA142480.15860481197737944No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTG95660.10648607744073638No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT2289850.091.647631
AGTGGTA2288400.091.2130055
TGGTATC2295000.090.901267
GGTATCA2298550.090.8220148
GTATCAA2301650.090.6462259
AGCAGTG2312450.090.6362842
GTGGTAT2308550.090.447876
GCAGTGG2320350.090.085713
CAGTGGT2319800.089.778864
CAACGCA2290850.046.1583612-13
ATCAACG2290850.046.14448510-11
ACGCAGA2301500.045.97704314-15
TCAACGC2291000.045.84531412-13
AACGCAG2298050.045.73908214-15
CGCAGAG2308450.045.59616-17
AGAGTAC2258650.045.4895518-19
TATCAAC2307600.045.40394610-11
GCAGAGT2325300.045.25844616-17
CAGAGTA2301900.045.1383318-19
GAGTACT1331550.045.12256220-21
Produced by FastQC (version 0.11.5)