FastQCFastQC Report
Sat 18 Jun 2016
SRR578598_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR578598_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8983334
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT155250.17282002428051768No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT101300.11276437011025083No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCACG3100.029.111613
TCAACGC82150.020.120510-11
ACTCGCG2050.019.69742850-51
ATCAACG82600.019.09084310-11
CAACGCA82950.019.06755312-13
CTCGCGT2400.018.8028252-53
CGCGTAG2150.018.77881254-55
AACGCAG88100.018.65379712-13
AGTGGTA104200.017.1393554
TCGCGTA2650.017.02896952-53
CGTAGCG2401.8189894E-1216.82226456-57
GGTATCA108150.016.4252577
AAGCAGT92500.015.9946671
ACGCAGA99200.015.96802614-15
CGCAGAG106050.015.563642514-15
TGGTATC113300.015.3433686
CGTACGC2657.2759576E-1215.23491920-21
TATCAAC116150.015.0894799
GTATCAA118000.014.7724038
GTGGTAT120400.014.7541645
Produced by FastQC (version 0.11.5)