FastQCFastQC Report
Sat 18 Jun 2016
SRR578597_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR578597_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9093757
Sequences flagged as poor quality0
Sequence length101
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT108412311.921618314630576No Hit
AAGCAGTGGTATCAACGCAGAGTATTTTTTTTTTTTTTTTTTTTTTTTTT253660.2789386168994839No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTG98150.10793118839661098No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA92040.10121229322490144No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT2326800.091.7602541
AGTGGTA2327500.091.236925
TGGTATC2336600.090.845217
GGTATCA2345350.090.791088
GTATCAA2352900.090.681029
AGCAGTG2351850.090.5464862
GTGGTAT2347450.090.513876
GCAGTGG2362000.089.964753
CAGTGGT2364800.089.7537164
ATCAACG2333200.046.1855310-11
CAACGCA2332850.046.16585512-13
ACGCAGA2341900.046.0242314-15
TCAACGC2335050.045.8588512-13
AACGCAG2340550.045.7777414-15
CGCAGAG2348100.045.5979916-17
AGAGTAC2301500.045.4833518-19
TATCAAC2358000.045.3894410-11
GCAGAGT2368450.045.2934516-17
CAGAGTA2344200.045.1452418-19
GAGTACT1366800.045.0862420-21
Produced by FastQC (version 0.11.5)