##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR578597_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 9093757 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.744954808007297 31.0 31.0 34.0 27.0 34.0 2 31.040517796989736 31.0 31.0 34.0 27.0 34.0 3 31.130434868668694 31.0 31.0 34.0 27.0 34.0 4 34.58425181143503 37.0 35.0 37.0 30.0 37.0 5 34.64346507169699 37.0 35.0 37.0 32.0 37.0 6 34.62967836065996 37.0 35.0 37.0 30.0 37.0 7 34.55562304996714 37.0 35.0 37.0 31.0 37.0 8 34.50586979616895 37.0 35.0 37.0 30.0 37.0 9 35.95076149494648 39.0 35.0 39.0 30.0 39.0 10-11 35.950243557200835 39.0 35.0 39.0 30.0 39.0 12-13 35.99356575065729 39.0 35.0 39.0 30.0 39.0 14-15 37.02903013572938 40.0 36.5 41.0 31.0 41.0 16-17 36.998244839839025 40.0 36.5 41.0 31.0 41.0 18-19 36.99638532237006 40.0 36.5 41.0 31.0 41.0 20-21 36.887009901408184 39.5 36.0 41.0 30.5 41.0 22-23 36.59591046912733 39.0 36.0 40.5 30.0 41.0 24-25 36.49200566938396 39.0 36.0 40.5 30.0 41.0 26-27 36.42839708604485 39.0 36.0 41.0 30.0 41.0 28-29 36.11152084886368 39.0 35.5 41.0 28.0 41.0 30-31 35.82705750769456 39.0 35.0 40.5 27.0 41.0 32-33 35.51676160909072 38.0 34.0 40.0 26.5 41.0 34-35 35.31365188227484 38.0 34.0 40.0 25.5 41.0 36-37 35.35475607056577 38.0 34.0 40.0 26.0 41.0 38-39 34.71393072192274 37.0 34.0 40.0 25.5 41.0 40-41 34.69456523854772 38.0 34.0 40.0 25.0 41.0 42-43 34.5372662256095 38.0 33.5 40.0 24.0 41.0 44-45 34.386442644112876 37.0 33.0 40.0 24.0 41.0 46-47 34.19562723085739 37.0 33.0 40.0 23.5 41.0 48-49 33.8572620755096 37.0 33.0 40.0 22.5 41.0 50-51 32.596229699122155 36.0 31.5 39.0 17.5 40.5 52-53 32.193203480145776 36.5 31.0 39.0 10.5 40.0 54-55 31.882254111254568 37.0 31.0 40.0 2.0 41.0 56-57 31.401308667033877 37.0 30.5 40.0 2.0 41.0 58-59 31.0789384409546 37.0 30.0 40.0 2.0 41.0 60-61 30.779881131637893 36.0 29.0 40.0 2.0 41.0 62-63 30.45967799667398 36.0 29.0 39.0 2.0 41.0 64-65 30.147169426233845 35.0 28.0 39.0 2.0 41.0 66-67 29.84727654367716 35.0 28.0 39.0 2.0 41.0 68-69 29.512374368481584 35.0 28.0 38.5 2.0 40.0 70-71 29.12525939498933 35.0 27.5 38.0 2.0 40.0 72-73 28.740863319747824 35.0 26.0 37.0 2.0 40.0 74-75 28.338021622966174 34.0 26.0 37.0 2.0 39.0 76-77 27.928591505139188 34.0 26.0 36.0 2.0 39.0 78-79 27.529471262537584 34.0 25.5 36.0 2.0 38.5 80-81 27.12613142180949 34.0 25.0 35.0 2.0 37.5 82-83 26.741163140822874 34.0 24.0 35.0 2.0 37.0 84-85 26.325497371438452 33.0 22.5 35.0 2.0 36.5 86-87 25.95154274520421 33.0 20.5 35.0 2.0 36.0 88-89 25.554121360401428 33.0 19.5 35.0 2.0 36.0 90-91 25.170979992097877 32.5 18.0 35.0 2.0 35.0 92-93 24.791965685909574 32.0 11.5 35.0 2.0 35.0 94-95 24.343593687405544 32.0 4.5 35.0 2.0 35.0 96-97 23.858736988463626 32.0 2.0 35.0 2.0 35.0 98-99 23.236522374635697 31.5 2.0 34.5 2.0 35.0 100-101 21.607287779957172 29.0 2.0 33.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.5234151263314537 1101 2 0.5487961067068703 1101 3 0.5929066355580446 1101 4 0.6751916193044707 1101 5 0.6124040491704292 1101 6 0.48593686451743423 1101 7 0.5894301501714736 1101 8 0.6064848038394217 1101 9 0.6332438915814507 1101 10-11 0.656023386250574 1101 12-13 0.6554291711406606 1101 14-15 0.8269138070839901 1101 16-17 0.7125580801834914 1101 18-19 0.7287295394574187 1101 20-21 0.7574398652690348 1101 22-23 0.8131557086284644 1101 24-25 0.9485535620239247 1101 26-27 0.8762955885320522 1101 28-29 0.9806277640287604 1101 30-31 1.058440834450785 1101 32-33 1.1257693207047907 1101 34-35 1.0701969126169715 1101 36-37 0.9833860878577312 1101 38-39 1.026740266251089 1101 40-41 0.9161251708732365 1101 42-43 1.1744977212571257 1101 44-45 1.0571583301694858 1101 46-47 1.0766505607634969 1101 48-49 1.1370675463099928 1101 50-51 1.1001979853611985 1101 52-53 1.1072463458188082 1101 54-55 0.9387528081133603 1101 56-57 0.8737610413612877 1101 58-59 0.9026641816468803 1101 60-61 1.0876884928308819 1101 62-63 1.1072203454000302 1101 64-65 1.0974322327656942 1101 66-67 0.9880175477910491 1101 68-69 1.0971454301299737 1101 70-71 0.964947925084342 1101 72-73 1.0795039263549207 1101 74-75 1.135905924973141 1101 76-77 1.067357986353251 1101 78-79 1.144290930853849 1101 80-81 1.214005782374322 1101 82-83 1.2819373742665157 1101 84-85 1.3147516226992657 1101 86-87 1.2617359211946955 1101 88-89 1.4111516108415927 1101 90-91 1.5424011161671736 1101 92-93 1.5853443517758024 1101 94-95 1.6827909098563403 1101 96-97 1.9265397707768344 1101 98-99 2.180287291507941 1101 100-101 2.6807438751425785 1102 1 0.47621262062825664 1102 2 0.3876956544762926 1102 3 0.43586032431954536 1102 4 0.5143903658460331 1102 5 0.4551372738073596 1102 6 0.40432582443830967 1102 7 0.4866140430656145 1102 8 0.530938429475718 1102 9 0.4271028118934552 1102 10-11 0.5713089115297905 1102 12-13 0.5686505579409626 1102 14-15 0.6774796239158363 1102 16-17 0.7043125311030067 1102 18-19 0.6537792988539124 1102 20-21 0.6633172698166376 1102 22-23 0.6709165661280636 1102 24-25 0.8096106432329933 1102 26-27 0.8332806804115833 1102 28-29 0.7475520272002214 1102 30-31 0.8466873421430776 1102 32-33 0.8499511120961643 1102 34-35 0.96625657596217 1102 36-37 0.810170953605386 1102 38-39 0.9380022886631707 1102 40-41 0.9236573553641989 1102 42-43 1.0578022353943055 1102 44-45 0.9446447765030328 1102 46-47 0.9198076463768743 1102 48-49 1.0408469326571463 1102 50-51 0.9695188622245681 1102 52-53 1.0229461139551645 1102 54-55 1.0576295566139642 1102 56-57 0.9314117343565691 1102 58-59 1.0092424549328882 1102 60-61 1.095449965463441 1102 62-63 1.118060007333387 1102 64-65 1.1517587129095084 1102 66-67 1.0997786954816995 1102 68-69 1.2690964069018413 1102 70-71 1.2201423436025998 1102 72-73 1.2877403159990486 1102 74-75 1.458426005212079 1102 76-77 1.237993834945872 1102 78-79 1.2688886765705476 1102 80-81 1.3501904402615494 1102 82-83 1.437004035952068 1102 84-85 1.4337237938474097 1102 86-87 1.4665700152147956 1102 88-89 1.6148885636820154 1102 90-91 1.5968245241971637 1102 92-93 1.5333896180705295 1102 94-95 1.5782944230833102 1102 96-97 1.8550256640359315 1102 98-99 2.005886444041998 1102 100-101 2.4165468139941915 1103 1 0.377327020415656 1103 2 0.29735161763040807 1103 3 0.22686747544428343 1103 4 0.35297254963224134 1103 5 0.30428140192118036 1103 6 0.2127495204202674 1103 7 0.4496402221417384 1103 8 0.3355348735330921 1103 9 0.6006368172436183 1103 10-11 0.48120707025126563 1103 12-13 0.36633818917336924 1103 14-15 0.5770235018258631 1103 16-17 0.5101510586032916 1103 18-19 0.5458379590822346 1103 20-21 0.6933845641514296 1103 22-23 0.6786962952020232 1103 24-25 0.5561345437580272 1103 26-27 0.7260008772414821 1103 28-29 0.7046755001211906 1103 30-31 0.599871716757562 1103 32-33 0.8378171100991452 1103 34-35 0.739841388105198 1103 36-37 0.7768656395566751 1103 38-39 0.5558569914974711 1103 40-41 0.7242494427518835 1103 42-43 0.576482481130796 1103 44-45 0.676307969351619 1103 46-47 0.7894628995762147 1103 48-49 0.7706109092285516 1103 50-51 0.7370090058428858 1103 52-53 0.6118517361256366 1103 54-55 0.6818875407284573 1103 56-57 0.7291300872470678 1103 58-59 0.8956972136351169 1103 60-61 0.8658676602914852 1103 62-63 0.830195040172633 1103 64-65 0.8749471086231289 1103 66-67 0.8306339860938685 1103 68-69 0.9721750253642902 1103 70-71 1.0881500409922786 1103 72-73 1.0851532906051808 1103 74-75 1.2069987628419803 1103 76-77 1.2323269426767887 1103 78-79 1.2339417753584954 1103 80-81 1.1247956640464025 1103 82-83 1.2680099133002045 1103 84-85 1.1848470366555368 1103 86-87 1.3899322358267376 1103 88-89 1.499527003972581 1103 90-91 1.237070004497916 1103 92-93 1.264276962524029 1103 94-95 1.3511224795185939 1103 96-97 1.5583167744516153 1103 98-99 1.6146968035922065 1103 100-101 1.9796630672731474 1104 1 0.2126086799470137 1104 2 0.26320125149615947 1104 3 0.23027048175807963 1104 4 0.23404538748025772 1104 5 0.13174402211004121 1104 6 0.11110939936422426 1104 7 0.29695895018355856 1104 8 0.26174323497780705 1104 9 0.389007393503789 1104 10-11 0.442203648927169 1104 12-13 0.29090448139027814 1104 14-15 0.40771898535802364 1104 16-17 0.5447204681210209 1104 18-19 0.579549404604883 1104 20-21 0.5119901898205725 1104 22-23 0.4115587966292438 1104 24-25 0.4212659350609371 1104 26-27 0.5008121271966175 1104 28-29 0.6364985626417692 1104 30-31 0.5816915281817714 1104 32-33 0.762325448726294 1104 34-35 0.6865050840735947 1104 36-37 0.7724285088674421 1104 38-39 0.5354979637825394 1104 40-41 0.6196422018630159 1104 42-43 0.6625252441337608 1104 44-45 0.922726356142455 1104 46-47 0.8295077000685112 1104 48-49 0.8077652906758743 1104 50-51 0.8184039158079628 1104 52-53 0.7160769251638399 1104 54-55 0.7964336386308197 1104 56-57 0.8990438198510411 1104 58-59 0.9572331963223348 1104 60-61 0.8878117076684937 1104 62-63 0.8072372259110594 1104 64-65 0.9963650999034535 1104 66-67 0.7832078407880978 1104 68-69 0.9083937351719484 1104 70-71 1.02698601333746 1104 72-73 0.9219426361939469 1104 74-75 1.0023882889372189 1104 76-77 1.0676879529163976 1104 78-79 1.1490619545035088 1104 80-81 1.035739596883566 1104 82-83 1.0534447845622097 1104 84-85 1.0941788291100671 1104 86-87 1.1258342468100828 1104 88-89 1.2525338613111714 1104 90-91 1.3611536970527247 1104 92-93 1.2528476919620246 1104 94-95 1.3051205336520972 1104 96-97 1.5170256412457555 1104 98-99 1.7269790106457101 1104 100-101 1.9680649306745757 1105 1 -0.1427876771579193 1105 2 -0.005316465096104395 1105 3 0.033248185030622324 1105 4 -0.01028303055293378 1105 5 0.07584816198319544 1105 6 0.08184694687985683 1105 7 0.15810570302024018 1105 8 0.1685415404286914 1105 9 0.05445274659352606 1105 10-11 0.1310543202480403 1105 12-13 0.08544768644688361 1105 14-15 -0.04992905736824582 1105 16-17 0.1599182111445714 1105 18-19 -6.79043094038434E-4 1105 20-21 0.04056526455281073 1105 22-23 0.1372136748743671 1105 24-25 0.26427776162802985 1105 26-27 0.2982600442733556 1105 28-29 0.4569934877457271 1105 30-31 0.3562273550391879 1105 32-33 0.5400017331836438 1105 34-35 0.4038384527469958 1105 36-37 0.6807733152767597 1105 38-39 0.4313339725077796 1105 40-41 0.37209403734250657 1105 42-43 0.633934858014868 1105 44-45 0.6662445716132837 1105 46-47 0.3825523560133419 1105 48-49 0.5063928904215018 1105 50-51 0.47237641915938866 1105 52-53 0.3444953633358807 1105 54-55 0.5008023522517142 1105 56-57 0.5435060006920622 1105 58-59 0.5685264622172355 1105 60-61 0.5206645145969198 1105 62-63 0.5235527069481094 1105 64-65 0.5746321516184061 1105 66-67 0.4621042216039015 1105 68-69 0.5830243591398592 1105 70-71 0.5829161388430926 1105 72-73 0.4819071056319437 1105 74-75 0.5164910715439 1105 76-77 0.4054117457727777 1105 78-79 0.5374374434158256 1105 80-81 0.6029498251296772 1105 82-83 0.7054006193510034 1105 84-85 0.7933116310845456 1105 86-87 0.766720718291019 1105 88-89 0.6406995214422331 1105 90-91 0.855097763646615 1105 92-93 0.9382139905581148 1105 94-95 1.0924537498338083 1105 96-97 1.2138466613961079 1105 98-99 1.5260990985696061 1105 100-101 1.869831842070031 1106 1 0.24450668796506037 1106 2 0.2820110653882928 1106 3 0.24824016853207098 1106 4 0.12272474931353372 1106 5 0.2982862802620403 1106 6 0.17390738727362987 1106 7 0.3099509054135794 1106 8 0.3187498252666856 1106 9 0.34604029150041526 1106 10-11 0.2281576384768087 1106 12-13 0.3498292781462524 1106 14-15 0.2940823324782613 1106 16-17 0.3727719924566415 1106 18-19 0.40335147842330343 1106 20-21 0.35367659274425023 1106 22-23 0.4494697423004368 1106 24-25 0.6008652386623936 1106 26-27 0.411372344466308 1106 28-29 0.5350433477356162 1106 30-31 0.6099421017598914 1106 32-33 0.5894920943643172 1106 34-35 0.5487648646922878 1106 36-37 0.648087066802006 1106 38-39 0.6598267886625777 1106 40-41 0.48750000968838947 1106 42-43 0.7074553753743942 1106 44-45 0.7350945547457073 1106 46-47 0.7730755257638435 1106 48-49 0.7987153187359723 1106 50-51 0.6502546203331931 1106 52-53 0.7205242311099909 1106 54-55 0.7699241271130397 1106 56-57 0.6401908052418328 1106 58-59 0.7533594849290601 1106 60-61 0.6564536264707677 1106 62-63 0.7524717960352305 1106 64-65 0.9195238396481571 1106 66-67 0.823046872007307 1106 68-69 0.9042150810839154 1106 70-71 0.9591415017473928 1106 72-73 0.8494189778760912 1106 74-75 0.8138951236840732 1106 76-77 0.9221143178395685 1106 78-79 1.0306512138615886 1106 80-81 0.9825639707009799 1106 82-83 1.146425467563752 1106 84-85 1.25361162091205 1106 86-87 1.2347257975016 1106 88-89 1.2601116147161662 1106 90-91 1.0495583941504663 1106 92-93 1.2788768687651526 1106 94-95 1.3385106497959143 1106 96-97 1.4673546902122645 1106 98-99 1.64648865665918 1106 100-101 1.8850711496764951 1107 1 0.1184693926493523 1107 2 0.14205783931381788 1107 3 0.2193799277231001 1107 4 0.09733997369100678 1107 5 0.2846778045464049 1107 6 0.2994715582489036 1107 7 0.2613646676275678 1107 8 0.2517204338802017 1107 9 0.20306160418425634 1107 10-11 0.2552271826062338 1107 12-13 0.2983613318715683 1107 14-15 0.2941286108899632 1107 16-17 0.22997200720381983 1107 18-19 0.23471826141211238 1107 20-21 0.29520210246245426 1107 22-23 0.4261304767744605 1107 24-25 0.40926536424989024 1107 26-27 0.3291651066108514 1107 28-29 0.4219331643783093 1107 30-31 0.4716056427124471 1107 32-33 0.42862647040492163 1107 34-35 0.37025088701040687 1107 36-37 0.33528720300919446 1107 38-39 0.3991803462691266 1107 40-41 0.4073327087425298 1107 42-43 0.4509891886574735 1107 44-45 0.37631128195965147 1107 46-47 0.520602825781026 1107 48-49 0.5549451045127682 1107 50-51 0.4894161528961902 1107 52-53 0.5144448461404636 1107 54-55 0.6281180179112518 1107 56-57 0.3450399669635722 1107 58-59 0.2647268891911665 1107 60-61 0.42289640902705017 1107 62-63 0.4702420880788267 1107 64-65 0.4851021386792489 1107 66-67 0.501078975928916 1107 68-69 0.4966889569715711 1107 70-71 0.5010985223184186 1107 72-73 0.5135626407701857 1107 74-75 0.5085044169437722 1107 76-77 0.5000152825759656 1107 78-79 0.5436400070801213 1107 80-81 0.5846298979019373 1107 82-83 0.5874526634262871 1107 84-85 0.6598336353982504 1107 86-87 0.6725587134639603 1107 88-89 0.8354513265270462 1107 90-91 0.8388508401765442 1107 92-93 0.9069734647248993 1107 94-95 0.9535114011281571 1107 96-97 1.109112607738556 1107 98-99 1.2430908183893017 1107 100-101 1.4411041433732592 1108 1 0.15163387537813477 1108 2 0.16305949182147472 1108 3 -0.01613921284473463 1108 4 0.15415545150285936 1108 5 0.2535596539876863 1108 6 0.21915419559557137 1108 7 0.24429203099658992 1108 8 0.15577853122113083 1108 9 0.19449929652728315 1108 10-11 0.1733374487699919 1108 12-13 0.23575678966532365 1108 14-15 0.16356912435303883 1108 16-17 0.260539248883326 1108 18-19 0.0838382823915893 1108 20-21 0.11909795068383744 1108 22-23 0.2395704005903312 1108 24-25 0.33173705958174793 1108 26-27 0.25585959992551466 1108 28-29 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2315 86-87 -1.8677981463680347 2315 88-89 -2.09042587293915 2315 90-91 -2.053744933384934 2315 92-93 -2.0854180580766197 2315 94-95 -2.2150553114887686 2315 96-97 -2.2007108357293355 2315 98-99 -2.4991631924032873 2315 100-101 -2.4461909833120146 2316 1 -1.3508502164258083 2316 2 -1.1890721711689132 2316 3 -2.3509580167629025 2316 4 -1.4880932737951582 2316 5 -1.2968059275770116 2316 6 -2.769203700359391 2316 7 -7.051209078943195 2316 8 -7.868208586985695 2316 9 -4.454372601039321 2316 10-11 -2.242373484242705 2316 12-13 -2.2598746905695677 2316 14-15 -2.3490106353693463 2316 16-17 -2.2804014550711074 2316 18-19 -1.7194530383450228 2316 20-21 -2.878614966531444 2316 22-23 -3.2736196072702697 2316 24-25 -2.0141084351070546 2316 26-27 -2.4282608804039256 2316 28-29 -3.2711211566271885 2316 30-31 -3.8171244555064376 2316 32-33 -3.795810288785077 2316 34-35 -2.8782756773335407 2316 36-37 -3.9533128996281093 2316 38-39 -3.5146324081651557 2316 40-41 -4.320285846257043 2316 42-43 -3.6274807637423905 2316 44-45 -3.2475101388467316 2316 46-47 -2.865027116856332 2316 48-49 -2.929567223816413 2316 50-51 -2.579924024278455 2316 52-53 -2.9656857601624047 2316 54-55 -4.381109850892724 2316 56-57 -3.118164702107901 2316 58-59 -2.5912141363554966 2316 60-61 -3.2287621338800143 2316 62-63 -2.960230855162024 2316 64-65 -4.435862692579928 2316 66-67 -3.399683291158805 2316 68-69 -3.272139365775555 2316 70-71 -3.71896599174951 2316 72-73 -3.4240271866225918 2316 74-75 -3.1453499004041667 2316 76-77 -3.589885893463258 2316 78-79 -4.639083319921731 2316 80-81 -3.7137353897387584 2316 82-83 -3.4212539474227874 2316 84-85 -3.548825348221552 2316 86-87 -4.643842387279317 2316 88-89 -5.12329446756754 2316 90-91 -4.518668420121447 2316 92-93 -4.672954209393318 2316 94-95 -4.614888897436657 2316 96-97 -4.887999493370696 2316 98-99 -5.038235909822113 2316 100-101 -5.248220008294236 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 75420.0 3 13662.0 4 12238.0 5 11881.0 6 13953.0 7 17339.0 8 21683.0 9 25883.0 10 30232.0 11 36478.0 12 38252.0 13 43828.0 14 50675.0 15 61134.0 16 76462.0 17 102842.0 18 148113.0 19 219318.0 20 284635.0 21 208253.0 22 126682.0 23 113438.0 24 119077.0 25 128112.0 26 141120.0 27 159703.0 28 182373.0 29 213197.0 30 250808.0 31 302139.0 32 365438.0 33 454997.0 34 581460.0 35 797950.0 36 1137005.0 37 1310641.0 38 1032845.0 39 184316.0 40 175.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 11.443622234675868 47.80828554032614 23.10446272365641 17.643629501341582 2 16.836117416081752 48.327414648409416 19.466532330243023 15.369935605265805 3 42.22554377380806 21.530039052816193 19.8541122584628 16.390304914912946 4 16.558931222123675 20.5475311654602 20.971384506128526 41.922153106287595 5 16.463829693554068 46.21409387108137 21.718639926030292 15.603436509334273 6 42.02514478519599 21.189636330757526 20.239957269119905 16.545261614926577 7 16.891324938757833 20.706507052687254 46.70083716435136 15.701330844203554 8 41.579516951432296 21.29753315637021 20.95944230639687 16.163507585800623 9 41.13918930564885 21.117207314108153 21.652008520278034 16.09159485996496 10-11 16.032142345395673 33.88811863906504 33.73454495990653 16.345194055632753 12-13 15.898581658309151 21.496034215919654 33.64729765895709 28.9580864668141 14-15 16.093698043058254 46.527441849357466 20.95949392456466 16.41936618301962 16-17 28.806466825343787 21.194014609086523 21.04456635770314 28.95495220786655 18-19 16.231874569490078 33.85592194593786 21.039512283149538 28.872691201422523 20-21 28.86599599932222 33.77665743151205 21.12495406262447 16.232392506541263 22-23 28.7884479835742 21.15019015328721 33.735772610192114 16.32558925294648 24-25 16.212647008748945 33.52439508404449 21.488137395472133 28.774820511734433 26-27 16.420263047257162 25.98427366833708 41.216989481289154 16.37847380311661 28-29 26.343025208946873 21.02225310295126 36.21980380224264 16.41491788585923 30-31 24.12022810363715 22.1968000014081 36.71377609470539 16.969195800249366 32-33 19.14435505613552 24.475431918852873 37.843496518722574 18.536716506289032 34-35 18.684828928576596 23.591416887609178 38.43564506015719 19.288109123657033 36-37 18.670066357228812 23.52903833591198 38.68975485645522 19.111140450403987 38-39 18.712411715340302 23.4632314695392 38.583561566215316 19.240795248905187 40-41 18.874403547602068 23.511764725622132 38.368385372682454 19.245446354093346 42-43 19.09278597322049 23.58221334878447 38.388640400726146 18.93636027726889 44-45 18.914949155690042 23.929292369194776 38.21692963474919 18.938828840365986 46-47 19.188832326302187 23.522980185088215 38.2074708052283 19.0807166833813 48-49 18.90505335910793 23.72442882218624 38.31240004398573 19.058117774720095 50-51 19.35192937063895 23.73165740924567 37.580997964121025 19.335415255994356 52-53 19.511314182562536 24.830868425299858 36.45435221503971 19.203465177097893 54-55 20.07419862938872 25.422654285082793 35.06910870220774 19.434038383320747 56-57 20.101376876564416 26.18684992969046 33.87477976526521 19.83699342847991 58-59 20.314496878927745 26.606469843717544 33.158020565982596 19.92101271137211 60-61 20.513671866512446 26.495923113858794 33.09108385614257 19.89932116348619 62-63 20.372811319660684 26.502227533304072 32.928340114424934 20.196621032610302 64-65 20.019161282415254 26.245703399279275 33.23559971755372 20.499535600751752 66-67 20.230886084082154 25.863274582893 33.71736419750234 20.188475135522506 68-69 20.134491038056286 25.950450091173373 33.66580764756198 20.24925122320837 70-71 19.868200147110528 25.943421474407018 34.21555273251118 19.972825645971273 72-73 19.684800714045682 25.707415864556193 34.125682667840316 20.482100753557813 74-75 19.88989715615236 25.91604120443008 33.50590043733307 20.68816120208449 76-77 20.46139926960114 26.256123878251604 33.05585650245527 20.226620349691984 78-79 20.214313824010677 26.25511022147713 33.00881545178929 20.521760502722902 80-81 20.120785233844533 26.58251579169784 32.57520567975773 20.721493294699897 82-83 20.94050256651713 26.949474925578702 31.969062082652194 20.14096042525198 84-85 20.522497936429655 27.150573110076248 31.53096978316902 20.795959170325073 86-87 20.36321069270028 27.648763605369016 31.411837010740605 20.576188691190097 88-89 20.244645047780036 27.414860155718518 31.281748816906614 21.058745979594836 90-91 20.363392512022223 27.204260896403916 31.689311360771367 20.743035230802498 92-93 20.250431132923595 27.687331050679404 31.484425089361896 20.577812727035102 94-95 20.54547973889257 27.180980252470157 31.134969333590107 21.13857067504717 96-97 20.116740167820453 27.038588628245275 31.65671464967775 21.187956554256523 98-99 20.660708424158635 27.409706742442175 31.177002417412407 20.75258241598678 100-101 21.042283577032347 26.860091479868274 31.009006921128655 21.08861802197072 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 11713.0 1 7115.0 2 1925.0 3 1223.5 4 1013.0 5 853.0 6 922.5 7 1076.5 8 1043.0 9 1087.5 10 1468.0 11 8586.5 12 158293.0 13 179818.0 14 53997.0 15 50884.0 16 54550.5 17 59577.0 18 64955.5 19 67008.5 20 68255.5 21 69089.5 22 70282.0 23 74736.0 24 80231.5 25 85462.0 26 92479.0 27 102871.5 28 112124.0 29 122408.5 30 134586.0 31 145425.0 32 157967.0 33 173555.5 34 193836.0 35 211993.5 36 224557.5 37 237070.0 38 248673.5 39 257531.5 40 265168.5 41 270917.0 42 274451.0 43 278973.5 44 285383.0 45 288190.0 46 286649.0 47 285704.5 48 285868.5 49 282135.5 50 274586.0 51 274174.0 52 272071.5 53 256339.5 54 231415.5 55 209491.0 56 197664.0 57 188856.0 58 200845.0 59 174579.5 60 116334.5 61 88112.5 62 64115.0 63 45185.0 64 31610.5 65 21867.5 66 14508.5 67 9721.0 68 6435.0 69 4125.0 70 2852.0 71 2240.5 72 1898.0 73 1712.5 74 1596.5 75 1432.5 76 1325.5 77 1269.5 78 1214.5 79 1155.5 80 1006.0 81 906.0 82 849.5 83 763.5 84 677.5 85 604.0 86 531.5 87 442.0 88 348.5 89 279.5 90 223.5 91 174.0 92 129.5 93 84.5 94 60.5 95 41.5 96 25.0 97 20.0 98 15.5 99 9.0 100 3.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.12301846200640726 2 0.11101022382718165 3 0.10610576024848695 4 0.09389958407729611 5 0.09849614411293374 6 0.0885882479595617 7 0.0981112646841124 8 0.09484528781668566 9 0.08948996547851455 10-11 0.07960406243536088 12-13 0.07175252208740568 14-15 0.07924667439431249 16-17 0.06961369211866998 18-19 0.06264737445700386 20-21 0.05824875241333148 22-23 0.05105150709437254 24-25 0.04810443032511205 26-27 0.04505838455986893 28-29 0.047345668022578564 30-31 0.038520932547460855 32-33 0.05098552776371745 34-35 0.06167417932984134 36-37 0.05162332796004996 38-39 0.053932604532977955 40-41 0.05403157352896059 42-43 0.04901714439917407 44-45 0.04883570123987258 46-47 0.04855528908458848 48-49 0.04889618229297307 50-51 0.05017178268563807 52-53 0.046581407442490494 54-55 0.046581407442490494 56-57 0.04736766113279693 58-59 0.05028724651428447 60-61 0.04575116753174733 62-63 0.055026761766341456 64-65 0.050529170726686445 66-67 0.04533879671515304 68-69 0.04323845468929948 70-71 0.05138140374764797 72-73 0.045553229539782074 74-75 0.04779102850450039 76-77 0.04320546502397194 78-79 0.04812092515777582 80-81 0.05021576890607479 82-83 0.03977453982990749 84-85 0.049863879142581 86-87 0.05267349897297674 88-89 0.04776353711672744 90-91 0.04427762914711709 92-93 0.041270071324756095 94-95 0.0449594155638863 96-97 0.03265976867426741 98-99 0.033484510307455986 100-101 0.024725754162993357 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 9093757.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.29128687629836 #Duplication Level Percentage of deduplicated Percentage of total 1 90.36941295935911 49.062717238192405 2 5.910308993011477 6.417565621343043 3 1.4593328660272427 2.3768717783248716 4 0.6713391401036691 1.4579146338662303 5 0.36757214865662674 0.9977982485227155 6 0.24679631990849388 0.8039333882500048 7 0.1522562102040144 0.5786329910818893 8 0.11828046237770105 0.5137278811847186 9 0.09634008092275015 0.4707384273956545 >10 0.5830252640967256 5.069643805235305 >50 0.008099432756980714 0.33841147556467177 >100 0.011910367876943025 1.2657359716342467 >500 0.002576978081733693 0.9766473163328241 >1k 0.002362229904876659 2.5009157316799335 >5k 3.006474424388475E-4 1.2977815628689022 >10k+ 8.589926926824214E-5 25.870963928522634 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 1084123 11.921618314630576 No Hit AAGCAGTGGTATCAACGCAGAGTATTTTTTTTTTTTTTTTTTTTTTTTTT 25366 0.2789386168994839 No Hit AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTG 9815 0.10793118839661098 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 9204 0.10121229322490144 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 1.0996555109180947E-5 0.0 3 1.0996555109180947E-5 0.0 0.0 1.0996555109180947E-5 0.0 4 1.0996555109180947E-5 0.0 0.0 1.0996555109180947E-5 0.0 5 1.0996555109180947E-5 0.0 0.0 1.0996555109180947E-5 0.0 6 1.0996555109180947E-5 0.0 0.0 2.1993110218361894E-5 0.0 7 1.0996555109180947E-5 0.0 0.0 3.298966532754284E-5 0.0 8 1.0996555109180947E-5 0.0 0.0 4.398622043672379E-5 0.0 9 1.0996555109180947E-5 0.0 0.0 4.398622043672379E-5 0.0 10-11 1.0996555109180947E-5 0.0 0.0 5.4982775545904735E-5 0.0 12-13 1.0996555109180947E-5 0.0 0.0 6.597933065508568E-5 0.0 14-15 1.0996555109180947E-5 0.0 0.0 8.797244087344758E-5 0.0 16-17 1.0996555109180947E-5 0.0 0.0 8.797244087344758E-5 0.0 18-19 1.0996555109180947E-5 0.0 0.0 8.797244087344758E-5 0.0 20-21 1.0996555109180947E-5 0.0 0.0 8.797244087344758E-5 0.0 22-23 1.0996555109180947E-5 0.0 0.0 8.797244087344758E-5 0.0 24-25 1.0996555109180947E-5 0.0 0.0 1.2096210620099041E-4 0.0 26-27 1.0996555109180947E-5 0.0 0.0 1.2096210620099041E-4 0.0 28-29 1.0996555109180947E-5 0.0 0.0 1.2096210620099041E-4 0.0 30-31 1.0996555109180947E-5 0.0 0.0 1.2096210620099041E-4 0.0 32-33 1.649483266377142E-5 0.0 0.0 1.2096210620099041E-4 0.0 34-35 4.398622043672379E-5 0.0 0.0 1.264603837555809E-4 0.0 36-37 5.4982775545904735E-5 0.0 0.0 1.3745693886476183E-4 0.0 38-39 6.048105310049521E-5 0.0 0.0 1.5395177152853324E-4 0.0 40-41 7.147760820967615E-5 0.0 0.0 1.5395177152853324E-4 0.0 42-43 9.347071842803805E-5 0.0 0.0 1.5395177152853324E-4 0.0 44-45 9.896899598262852E-5 0.0 0.0 1.5395177152853324E-4 0.0 46-47 1.1546382864639994E-4 0.0 0.0 1.5395177152853324E-4 0.0 48-49 1.429552164193523E-4 0.0 0.0 1.5395177152853324E-4 0.0 50-51 1.5945004908312374E-4 0.0 0.0 1.5945004908312374E-4 0.0 52-53 2.254293797382094E-4 0.0 0.0 1.649483266377142E-4 0.0 54-55 2.4192421240198082E-4 0.0 0.0 1.649483266377142E-4 0.0 56-57 2.5841904506575226E-4 0.0 0.0 1.8144315930148562E-4 0.0 58-59 3.353949308300189E-4 0.0 0.0 1.869414368560761E-4 0.0 60-61 3.848794288213331E-4 0.0 0.0 1.869414368560761E-4 0.0 62-63 4.6185531458559976E-4 0.0 0.0 1.9243971441066656E-4 0.0 64-65 5.003432574677331E-4 0.0 0.0 1.9793799196525703E-4 0.0 66-67 5.938139758957711E-4 0.0 0.0 1.9793799196525703E-4 0.0 68-69 7.092778045421711E-4 0.0 0.0 1.9793799196525703E-4 0.0 70-71 8.302399107431614E-4 0.0 0.0 1.9793799196525703E-4 1.0996555109180947E-5 72-73 9.51202016944152E-4 0.0 0.0 1.9793799196525703E-4 1.0996555109180947E-5 74-75 0.0011326451762456375 0.0 0.0 1.9793799196525703E-4 1.0996555109180947E-5 76-77 0.0013525762784292565 0.0 0.0 2.08934547074438E-4 1.0996555109180947E-5 78-79 0.001649483266377142 5.4982775545904735E-6 0.0 2.1443282462902847E-4 1.0996555109180947E-5 80-81 0.0020123695849801134 1.0996555109180947E-5 0.0 2.254293797382094E-4 1.0996555109180947E-5 82-83 0.0024852214546748942 1.0996555109180947E-5 0.0 2.3642593484739035E-4 1.0996555109180947E-5 84-85 0.0029360802141513128 1.0996555109180947E-5 0.0 2.529207675111618E-4 1.0996555109180947E-5 86-87 0.003694842516684798 1.0996555109180947E-5 0.0 2.529207675111618E-4 1.0996555109180947E-5 88-89 0.00465154281118354 1.0996555109180947E-5 0.0 2.529207675111618E-4 1.0996555109180947E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AAGCAGT 232680 0.0 91.760254 1 AGTGGTA 232750 0.0 91.23692 5 TGGTATC 233660 0.0 90.84521 7 GGTATCA 234535 0.0 90.79108 8 GTATCAA 235290 0.0 90.68102 9 AGCAGTG 235185 0.0 90.546486 2 GTGGTAT 234745 0.0 90.51387 6 GCAGTGG 236200 0.0 89.96475 3 CAGTGGT 236480 0.0 89.753716 4 ATCAACG 233320 0.0 46.18553 10-11 CAACGCA 233285 0.0 46.165855 12-13 ACGCAGA 234190 0.0 46.02423 14-15 TCAACGC 233505 0.0 45.85885 12-13 AACGCAG 234055 0.0 45.77774 14-15 CGCAGAG 234810 0.0 45.59799 16-17 AGAGTAC 230150 0.0 45.48335 18-19 TATCAAC 235800 0.0 45.38944 10-11 GCAGAGT 236845 0.0 45.29345 16-17 CAGAGTA 234420 0.0 45.14524 18-19 GAGTACT 136680 0.0 45.08624 20-21 >>END_MODULE