FastQCFastQC Report
Sat 18 Jun 2016
SRR578597_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR578597_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9093757
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT153450.16874213815038164No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT101510.11162603091329579No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCACG3500.023.0597523
ACTCGCG1850.021.81759650-51
TCAACGC85400.019.48483510-11
CTCGCGT1950.019.47994252-53
CAACGCA85750.019.21152912-13
ATCAACG85700.019.11194410-11
AACGCAG90450.018.3969612-13
AAGCAGT95150.016.8417761
AGTGGTA108050.016.4331424
GGTATCA110800.016.0251037
ACGCAGA103700.015.81742914-15
TGGTATC112600.015.8110916
CGCGTAG2452.910383E-1115.50307954-55
CGCAGAG109300.015.28935314-15
CGTACGC2801.8189894E-1215.26029920-21
TATCAAC117700.015.0856539
GTATCAA121950.014.5209838
AGCAGTG128300.014.4545561
CGTAGCG3003.6379788E-1214.24274956-57
GTGGTAT126800.014.1154885
Produced by FastQC (version 0.11.5)