##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR578597_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 9093757 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.438040515047852 31.0 31.0 34.0 30.0 34.0 2 31.810014606724152 31.0 31.0 34.0 30.0 34.0 3 31.886798492636213 31.0 31.0 34.0 30.0 34.0 4 35.5838413100328 37.0 35.0 37.0 33.0 37.0 5 35.410776426069006 37.0 35.0 37.0 33.0 37.0 6 35.40777161738542 37.0 35.0 37.0 33.0 37.0 7 35.4008099182769 37.0 35.0 37.0 33.0 37.0 8 35.361160629209685 37.0 35.0 37.0 32.0 37.0 9 36.91160936013575 39.0 37.0 39.0 33.0 39.0 10-11 36.90896435873534 39.0 37.0 39.0 33.0 39.0 12-13 36.90210041900174 39.0 37.0 39.0 33.0 39.0 14-15 38.088775409327525 40.0 37.0 41.0 33.0 41.0 16-17 38.02400982344261 40.0 37.0 41.0 33.0 41.0 18-19 37.9580197711463 40.0 37.0 41.0 33.0 41.0 20-21 37.91614021575461 40.0 37.0 41.0 33.0 41.0 22-23 37.71512929144687 40.0 37.0 41.0 32.0 41.0 24-25 37.729529830190096 40.0 37.0 41.0 32.0 41.0 26-27 37.565868430396804 39.0 36.5 41.0 32.0 41.0 28-29 37.42073930499792 39.0 36.0 41.0 32.0 41.0 30-31 37.286043161258874 39.0 36.0 41.0 31.0 41.0 32-33 37.147625783270875 39.0 36.0 41.0 31.0 41.0 34-35 37.00527691690024 39.0 36.0 40.0 30.5 41.0 36-37 36.85061135897957 39.0 35.5 40.0 30.0 41.0 38-39 36.683996779328936 39.0 35.0 40.0 30.0 41.0 40-41 36.52710606848193 38.0 35.0 40.0 30.0 41.0 42-43 36.61687078288985 39.0 35.0 40.0 30.0 41.0 44-45 36.55555426651493 39.0 35.0 40.0 30.0 41.0 46-47 36.409077183390764 38.5 35.0 40.0 30.0 41.0 48-49 36.26457123276991 38.0 35.0 40.0 30.0 41.0 50-51 36.082185393781685 38.0 35.0 40.0 29.0 41.0 52-53 36.12132466262294 38.0 35.0 40.0 29.0 41.0 54-55 36.113124146598594 38.0 35.0 40.0 29.0 41.0 56-57 35.963836783850724 38.0 35.0 40.0 28.5 41.0 58-59 35.750313429312 38.0 34.0 40.0 28.0 41.0 60-61 35.50649687472406 38.0 34.0 40.0 28.0 41.0 62-63 35.24318997087782 38.0 34.0 40.0 28.0 41.0 64-65 34.93655603509089 37.0 34.0 40.0 27.5 41.0 66-67 34.61183199639049 37.0 33.0 39.5 26.5 41.0 68-69 34.24838870227124 36.0 33.0 39.0 26.0 41.0 70-71 33.867675813198005 36.0 32.5 39.0 26.0 40.5 72-73 33.48487385356789 35.0 32.0 38.5 26.0 40.0 74-75 33.07983801414531 35.0 32.0 37.5 25.5 40.0 76-77 31.69644702404078 34.0 30.5 36.0 23.5 39.0 78-79 32.35078559939528 35.0 31.0 37.0 25.0 39.0 80-81 32.217415200340184 35.0 31.0 36.0 25.0 39.0 82-83 31.839097910797484 35.0 31.0 36.0 24.0 37.5 84-85 31.54656551742036 34.0 31.0 35.5 24.0 37.0 86-87 31.221853245033927 34.0 31.0 35.0 23.0 37.0 88-89 30.904348279814386 34.0 31.0 35.0 21.5 36.0 90-91 30.557316629419503 34.0 31.0 35.0 20.0 36.0 92-93 30.187272433164864 34.0 30.0 35.0 18.5 36.0 94-95 29.8094557617935 34.0 30.0 35.0 16.0 35.0 96-97 29.348047567138643 34.0 30.0 35.0 4.5 35.0 98-99 28.534375231271298 34.0 29.0 35.0 2.0 35.0 100-101 27.49323992272941 33.0 27.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.37326616321709594 1101 2 0.24451634943057954 1101 3 0.3443386887411144 1101 4 0.3036413343088995 1101 5 0.2497948761549864 1101 6 0.20183364425147232 1101 7 0.2217417058041704 1101 8 0.16391511833381145 1101 9 0.3187921033124397 1101 10-11 0.30298581443116035 1101 12-13 0.2952860462971998 1101 14-15 0.28308063525248883 1101 16-17 0.3631691588674144 1101 18-19 0.4209271051113106 1101 20-21 0.37902197412226 1101 22-23 0.42028838556093007 1101 24-25 0.36818362817894723 1101 26-27 0.3617072499211389 1101 28-29 0.2679453734860431 1101 30-31 0.2917079951588022 1101 32-33 0.4064892702417211 1101 34-35 0.41976964942751493 1101 36-37 0.38278508740197026 1101 38-39 0.3273227693355878 1101 40-41 0.30153207924769276 1101 42-43 0.32566687749836376 1101 44-45 0.298835993110103 1101 46-47 0.3268433342775552 1101 48-49 0.3583345278075498 1101 50-51 0.39044246099319224 1101 52-53 0.4559476910290172 1101 54-55 0.5117541042123648 1101 56-57 0.565527380734899 1101 58-59 0.5429831676428023 1101 60-61 0.515585485755004 1101 62-63 0.42253394512380993 1101 64-65 0.43368980039954863 1101 66-67 0.5098073401697434 1101 68-69 0.44551268107750985 1101 70-71 0.5054880600634419 1101 72-73 0.6269561705679934 1101 74-75 0.6205210438819293 1101 76-77 0.4410648882385715 1101 78-79 0.5100749391940411 1101 80-81 0.43956533505032525 1101 82-83 0.5842211410084523 1101 84-85 0.5527359794346616 1101 86-87 0.5659187150854379 1101 88-89 0.6125032335122498 1101 90-91 0.5935959670570874 1101 92-93 0.6729785660040974 1101 94-95 0.6146185218564142 1101 96-97 0.6196536418493501 1101 98-99 0.998498247717098 1101 100-101 1.1590426225230424 1102 1 0.24830003108634813 1102 2 0.2612011729888799 1102 3 0.27137829064508523 1102 4 0.28454368433486366 1102 5 0.27387017250565293 1102 6 0.1903057682512781 1102 7 0.2771571135026747 1102 8 0.1599122384211853 1102 9 0.2507392572522704 1102 10-11 0.2743231196349072 1102 12-13 0.2924897514503897 1102 14-15 0.3764588546438432 1102 16-17 0.2607667553402919 1102 18-19 0.32400948893141646 1102 20-21 0.296513426618759 1102 22-23 0.4558465133493925 1102 24-25 0.3483617391551874 1102 26-27 0.4724630878410281 1102 28-29 0.40126860828795685 1102 30-31 0.4389702019448336 1102 32-33 0.47742594610875244 1102 34-35 0.40435391982128266 1102 36-37 0.4676064577702732 1102 38-39 0.4299945987258198 1102 40-41 0.3837799715698864 1102 42-43 0.32451605737561096 1102 44-45 0.336521775706899 1102 46-47 0.4552593785379244 1102 48-49 0.35348362963521396 1102 50-51 0.35409182506423775 1102 52-53 0.3974675819160467 1102 54-55 0.49424996267271126 1102 56-57 0.5477483553605609 1102 58-59 0.5473164948445657 1102 60-61 0.6053947665680965 1102 62-63 0.4048470948742917 1102 64-65 0.43020798195180987 1102 66-67 0.42110823211015713 1102 68-69 0.45549269767163736 1102 70-71 0.4631808926290901 1102 72-73 0.49152786546338234 1102 74-75 0.43275638559551766 1102 76-77 0.26981020878352524 1102 78-79 0.43686678711554094 1102 80-81 0.5770016403442 1102 82-83 0.6160816441677675 1102 84-85 0.5597775983583979 1102 86-87 0.5909282609058764 1102 88-89 0.4156809973117177 1102 90-91 0.4959077812934787 1102 92-93 0.5237847528525243 1102 94-95 0.5687345577870033 1102 96-97 0.7198450868694302 1102 98-99 0.9793594229476881 1102 100-101 1.0400227578295365 1103 1 0.19831575957469738 1103 2 0.24315738506811968 1103 3 0.24284246020387812 1103 4 0.19922307198247324 1103 5 0.23971932349829217 1103 6 0.057117124367628946 1103 7 0.2174557927087264 1103 8 0.08128669038339353 1103 9 0.22680313558998932 1103 10-11 0.19870512361011805 1103 12-13 0.271156663486984 1103 14-15 0.3228808588578005 1103 16-17 0.28067073645879503 1103 18-19 0.26013020879391036 1103 20-21 0.3953449657882615 1103 22-23 0.3849790129578494 1103 24-25 0.31986048148694834 1103 26-27 0.16296301688627324 1103 28-29 0.166176905156604 1103 30-31 0.19335994061085415 1103 32-33 0.16474312068375951 1103 34-35 0.16400092477027073 1103 36-37 0.2296056794727548 1103 38-39 0.3658406794142195 1103 40-41 0.2780343722859797 1103 42-43 0.2978441548991455 1103 44-45 0.27839101169469416 1103 46-47 0.3517439761668655 1103 48-49 0.2411323793835649 1103 50-51 0.2609717917589549 1103 52-53 0.2259331545255776 1103 54-55 0.34878975287444547 1103 56-57 0.21440116887281135 1103 58-59 0.21242013631568568 1103 60-61 0.26008694224405104 1103 62-63 0.3465143218529647 1103 64-65 0.21872712089324864 1103 66-67 0.16996788616346237 1103 68-69 0.3390768167303335 1103 70-71 0.21038410771198102 1103 72-73 0.21949335393594538 1103 74-75 0.22247212759301505 1103 76-77 0.15970006887600974 1103 78-79 0.3382439236887649 1103 80-81 0.31884756487047383 1103 82-83 0.5620681931659242 1103 84-85 0.35984986086407034 1103 86-87 0.26239169863649536 1103 88-89 0.2892684889464512 1103 90-91 0.43913195235176516 1103 92-93 0.44858183371210103 1103 94-95 0.5030241248829483 1103 96-97 0.45893787729500346 1103 98-99 0.9424515014638466 1103 100-101 0.7791969219633756 1104 1 0.2556744789270411 1104 2 0.23475037980407976 1104 3 0.16709355314631225 1104 4 0.1723735812237237 1104 5 0.21138034016551188 1104 6 0.15666386480506134 1104 7 0.17428665695659618 1104 8 0.13282601502140068 1104 9 0.2746785299817702 1104 10-11 0.10465051554204052 1104 12-13 0.18782536232431113 1104 14-15 0.2876609588573231 1104 16-17 0.32699580311022913 1104 18-19 0.11782240339629624 1104 20-21 0.2262618448026288 1104 22-23 0.3250440850587424 1104 24-25 0.25684493918960527 1104 26-27 0.3362970848144613 1104 28-29 0.29327714505745917 1104 30-31 0.25521314532628026 1104 32-33 0.25399929356316164 1104 34-35 0.22957042532321736 1104 36-37 0.26653474988384573 1104 38-39 0.2534782232785986 1104 40-41 0.2844738992264766 1104 42-43 0.2300172329192307 1104 44-45 0.1967669655417339 1104 46-47 0.2332364111130758 1104 48-49 0.23926409509549273 1104 50-51 0.2890381553485568 1104 52-53 0.16698182170939901 1104 54-55 0.21477381398177187 1104 56-57 0.12360568604578503 1104 58-59 0.1261890990104959 1104 60-61 0.1836923755820834 1104 62-63 0.14147836339359543 1104 64-65 0.16639162795200235 1104 66-67 0.2076028863302568 1104 68-69 0.25295497422632707 1104 70-71 0.26807405581467947 1104 72-73 0.22636472184994005 1104 74-75 0.27552357365627955 1104 76-77 0.17501012244575875 1104 78-79 0.2395565767343797 1104 80-81 0.3136731912935744 1104 82-83 0.33664335159839354 1104 84-85 0.20551653021267668 1104 86-87 0.34019593820177363 1104 88-89 0.3829715898753996 1104 90-91 0.29042636332271243 1104 92-93 0.3796311081740633 1104 94-95 0.3878776420290748 1104 96-97 0.37472154791819534 1104 98-99 0.7695112376985662 1104 100-101 0.808614886213384 1105 1 0.26024065457048806 1105 2 0.17737894771351748 1105 3 0.07046879363746328 1105 4 0.15162478009342095 1105 5 0.19079459653598008 1105 6 0.049460990323275666 1105 7 0.188259420041625 1105 8 0.044493929676377775 1105 9 0.19505253189655747 1105 10-11 0.09194156121235864 1105 12-13 0.12557683785102114 1105 14-15 0.17068775110292478 1105 16-17 0.11224978162727695 1105 18-19 0.21429372663404678 1105 20-21 0.12176313541620942 1105 22-23 0.23373325343425932 1105 24-25 0.2571318396201363 1105 26-27 0.22589627988462269 1105 28-29 0.22133466890204545 1105 30-31 0.12148576239939501 1105 32-33 0.2184240757059399 1105 34-35 0.1457174946819535 1105 36-37 0.173304666768189 1105 38-39 0.23903789445041923 1105 40-41 0.19603366130507283 1105 42-43 0.20360957631637433 1105 44-45 0.2976682796218313 1105 46-47 0.27206103667359827 1105 48-49 0.21246406087762892 1105 50-51 0.38176602837557283 1105 52-53 0.31853688505318445 1105 54-55 0.3252314526068929 1105 56-57 0.2894166232379476 1105 58-59 0.21884032460469882 1105 60-61 0.17605395069175245 1105 62-63 0.24859724301321506 1105 64-65 0.29356495683094863 1105 66-67 0.3613358657471366 1105 68-69 0.3289858168682187 1105 70-71 0.3338605456941579 1105 72-73 0.40518813743194926 1105 74-75 0.3995179939539426 1105 76-77 0.2999568747937502 1105 78-79 0.37461104454609995 1105 80-81 0.44630659911705806 1105 82-83 0.5350117833585522 1105 84-85 0.43173855194830324 1105 86-87 0.411818023449964 1105 88-89 0.4110939464411345 1105 90-91 0.6199375288360969 1105 92-93 0.729935860765135 1105 94-95 0.6826290695005461 1105 96-97 0.7512160399367254 1105 98-99 1.2967368006349602 1105 100-101 1.3875899721947107 1106 1 0.1344382882831212 1106 2 0.10537388123950464 1106 3 0.1090248083240688 1106 4 0.08554007418504028 1106 5 0.07215118885961402 1106 6 -0.04958904579999057 1106 7 0.029536435608228828 1106 8 -0.02969482195361195 1106 9 0.04308649966197464 1106 10-11 -0.03449141618408902 1106 12-13 0.02703777285948661 1106 14-15 -0.012931627188159212 1106 16-17 0.031080282833137574 1106 18-19 0.07871342749555055 1106 20-21 0.10766825902044985 1106 22-23 0.217930084407719 1106 24-25 0.13940821349816446 1106 26-27 0.14944933415632988 1106 28-29 0.119892637646835 1106 30-31 0.10352853750389102 1106 32-33 0.08931993764419133 1106 34-35 0.1401042363133982 1106 36-37 0.024409773814880964 1106 38-39 0.080188147614912 1106 40-41 0.156463983126379 1106 42-43 0.1308237580193179 1106 44-45 0.06885572525050065 1106 46-47 0.03948678623338964 1106 48-49 0.04495473594774779 1106 50-51 0.07737147553898893 1106 52-53 0.11558218484776717 1106 54-55 0.11472889263983888 1106 56-57 0.1495713826348961 1106 58-59 0.04397918255992295 1106 60-61 0.18686679699870723 1106 62-63 0.20306123530156128 1106 64-65 0.13793707654332366 1106 66-67 0.051074714082901096 1106 68-69 0.14989727272702424 1106 70-71 0.18559076701387056 1106 72-73 0.2009151853343596 1106 74-75 0.27828584463573947 1106 76-77 0.16385420864391875 1106 78-79 0.3022138425026881 1106 80-81 0.41216238191427124 1106 82-83 0.5287552778614604 1106 84-85 0.5133943196292243 1106 86-87 0.5476453785551243 1106 88-89 0.601242878856695 1106 90-91 0.6069919939344537 1106 92-93 0.6191393708971624 1106 94-95 0.6413084814530485 1106 96-97 0.6953710011077767 1106 98-99 1.0180896960011765 1106 100-101 1.009699805347303 1107 1 0.20724704767772906 1107 2 0.11253869945687356 1107 3 0.04771686034678524 1107 4 0.02773715024501655 1107 5 0.02039502766649548 1107 6 0.02778403010692898 1107 7 0.017814255270003798 1107 8 0.062217446517287556 1107 9 0.11217099433568478 1107 10-11 0.040413834263226533 1107 12-13 0.06935144515006897 1107 14-15 0.06686942347312907 1107 16-17 0.07767930057640626 1107 18-19 0.008796775930768774 1107 20-21 0.0820755718156434 1107 22-23 0.20188681390468588 1107 24-25 0.1640152043921077 1107 26-27 0.18045546154486658 1107 28-29 0.11787168289380645 1107 30-31 0.05665459557639707 1107 32-33 0.16713961016651524 1107 34-35 0.21017249253682735 1107 36-37 0.17297987784269253 1107 38-39 0.21545150730184304 1107 40-41 0.12795715868639235 1107 42-43 0.16520153453591746 1107 44-45 0.24565646119064866 1107 46-47 0.19625460758700086 1107 48-49 0.10961931178253792 1107 50-51 0.1804110551626863 1107 52-53 0.0965751397375243 1107 54-55 0.12404404738741448 1107 56-57 0.20226459701911637 1107 58-59 0.12425048766594671 1107 60-61 0.18043557034079072 1107 62-63 0.2557908930616648 1107 64-65 0.22710296769323435 1107 66-67 0.17646649784023793 1107 68-69 0.2147542766806012 1107 70-71 0.19212913107013208 1107 72-73 0.19379690887117818 1107 74-75 0.34200675684552806 1107 76-77 0.1942363809378378 1107 78-79 0.20467813702320825 1107 80-81 0.24987003821556897 1107 82-83 0.4369123275828848 1107 84-85 0.46295422596098845 1107 86-87 0.5496131972430689 1107 88-89 0.48526759371052464 1107 90-91 0.5507222499656983 1107 92-93 0.5973003567826503 1107 94-95 0.5469581129777907 1107 96-97 0.5946931736639662 1107 98-99 1.0127807651652354 1107 100-101 1.1351081151894107 1108 1 0.03873591755263206 1108 2 -0.05253249082933209 1108 3 -0.04066120392262107 1108 4 0.00371846953790822 1108 5 0.029845843932633898 1108 6 0.017700678347118526 1108 7 -0.06544967701456983 1108 8 0.01699062996058842 1108 9 -0.03718004123221874 1108 10-11 -0.042536500778169284 1108 12-13 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-1.392162399681041 2316 90-91 -1.296755166328797 2316 92-93 -1.4317410433472624 2316 94-95 -1.5314973559579457 2316 96-97 -1.6743875598518088 2316 98-99 -1.2827602582591418 2316 100-101 -1.6012283281188324 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 149.0 3 75.0 4 242.0 5 559.0 6 1097.0 7 2029.0 8 3616.0 9 5698.0 10 7875.0 11 10007.0 12 12327.0 13 15226.0 14 17525.0 15 19935.0 16 23522.0 17 27858.0 18 33007.0 19 37601.0 20 40224.0 21 41580.0 22 46286.0 23 54739.0 24 68271.0 25 86154.0 26 109359.0 27 139792.0 28 176757.0 29 218675.0 30 269128.0 31 328047.0 32 401765.0 33 499940.0 34 643397.0 35 892467.0 36 1156158.0 37 1646514.0 38 1646258.0 39 409039.0 40 859.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 17.9739758566901 32.00059169811853 28.04673313687898 21.97869930831239 2 22.36601439866933 30.140084015880348 28.132223018494994 19.36167856695533 3 21.127098513848566 28.99934537507435 28.616995153928126 21.256560957148952 4 21.281629405232692 29.296871649456534 28.834759196338027 20.586739748972747 5 21.701444979345027 29.3045158546131 28.7071568495707 20.28688231647117 6 21.561241855393398 29.139459329640538 28.29136017404485 21.007938640921214 7 21.6414258599872 29.19872351749665 28.664059012023657 20.495791610492493 8 20.94622717541276 29.21345929960521 28.667722262646784 21.172591262335246 9 20.653278112916823 29.10289476120821 29.210672223017642 21.03315490285733 10-11 20.77177516634664 29.53303628272053 28.595726300184747 21.099462250748083 12-13 20.76495996099302 29.623762763838972 28.407972634412815 21.203304640755192 14-15 20.98284570392633 29.51975184733878 28.272786484178102 21.224615964556783 16-17 21.172814685857784 29.350481217578373 28.219232417234004 21.257471679329843 18-19 21.15794385092982 29.43620551989678 28.215978280484073 21.189872348689327 20-21 21.231248054640567 29.303038848687006 28.307108289077238 21.15860480759519 22-23 21.249366938204425 29.282201532566248 28.243059220714468 21.225372308514856 24-25 21.143890253500285 29.22297681805221 28.319873733155614 21.31325919529189 26-27 21.396447317431715 29.069136290119324 28.434529964982534 21.09988642746643 28-29 21.370858768141932 29.118555730844047 28.329383614584575 21.18120188642944 30-31 21.333995058177866 29.12944510236922 28.3105166193485 21.226043220104412 32-33 21.36647890206284 29.157208012606905 28.199533206590534 21.27677987873972 34-35 21.34761366266189 29.17897490172977 28.225399974454774 21.248011461153574 36-37 21.36387049155306 29.202675080396247 28.171335609192642 21.26211881885805 38-39 21.383095405297283 29.175745350104897 28.174419809172253 21.26673943542557 40-41 21.383875759369417 29.20479035397055 28.16064692487174 21.250686961788293 42-43 21.384667551203457 29.24505494136924 28.13958005964273 21.23069744778457 44-45 21.426324579550375 29.235956260867773 28.11836782383384 21.219351335748012 46-47 21.44038042680342 29.24911566054782 28.080561358283813 21.22994255436495 48-49 21.452159551230547 29.24748107160775 28.04168480342032 21.25867457374138 50-51 21.48167491419524 29.250935909461173 28.024732578051292 21.242656598292296 52-53 21.42142300124076 29.319879400504345 27.987779256972534 21.270918341282364 54-55 21.454407829317844 29.31270885098171 27.93510341677072 21.297779902929726 56-57 21.49440204871865 29.294480652725564 27.91136133606578 21.29975596249001 58-59 21.479736305828233 29.419944630536033 27.859859865804022 21.240459197831715 60-61 21.47047812419433 29.389020917813223 27.850080437055908 21.290420520936536 62-63 21.479788742678483 29.406617340448555 27.85737095744063 21.256222959432332 64-65 21.465616808521705 29.455067275896106 27.835303732606175 21.24401218297601 66-67 21.49701242141221 29.459226062312478 27.822897775283238 21.22086374099207 68-69 21.44673433555903 29.51328719191985 27.793359736092636 21.246618736428484 70-71 21.512451706276508 29.54790157840048 27.737271147435667 21.20237556788734 72-73 21.4592390313705 29.526798452850606 27.786263630249703 21.22769888552919 74-75 21.509784697667687 29.53994427815768 27.75201443350881 21.198256590665824 76-77 21.457310169043176 29.620502762182753 27.74911453063787 21.1730725381362 78-79 21.454717323379242 29.599409607762723 27.71948077197678 21.226392296881258 80-81 21.458565785046837 29.636454638080757 27.712421118078606 21.1925584587938 82-83 21.5450483686053 29.711663322814342 27.584062709918626 21.15922559866173 84-85 21.448694743857914 29.715997847533 27.672495779107226 21.162811629501864 86-87 21.412605687222346 29.775370046861642 27.624929373234608 21.187094892681408 88-89 21.449716277288832 29.779310774073327 27.63088732048361 21.14008562815423 90-91 21.43444168568586 29.783689488840224 27.61786820020336 21.164000625270553 92-93 21.423734043531784 29.82902771765466 27.581707525675863 21.165530713137695 94-95 21.40020516458996 29.86611737642468 27.578873644531704 21.15480381445366 96-97 21.380147950403444 29.888948399243848 27.58461105107841 21.146292599274297 98-99 21.443346757343225 29.888461468728483 27.486644164093377 21.18154760983492 100-101 21.489089115749454 29.853166582002206 27.47587750057306 21.181866801675284 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 125830.0 1 71846.0 2 12989.5 3 7326.5 4 6202.5 5 5325.0 6 4915.0 7 4974.5 8 4953.0 9 4494.5 10 4013.0 11 4752.5 12 8958.0 13 9138.0 14 5930.5 15 6428.5 16 7742.5 17 10756.5 18 16795.0 19 20198.5 20 23208.5 21 27030.5 22 32121.0 23 41455.5 24 51390.0 25 61099.0 26 74722.5 27 91974.0 28 104807.5 29 118804.0 30 136625.5 31 153619.0 32 172351.5 33 193480.0 34 214439.0 35 234929.5 36 256636.0 37 274904.5 38 288788.5 39 297894.0 40 304668.5 41 310980.0 42 312714.5 43 315904.5 44 321510.0 45 326027.0 46 326766.0 47 324961.0 48 326092.5 49 323649.0 50 312925.5 51 300847.0 52 290434.5 53 272476.0 54 246660.0 55 225541.0 56 205566.0 57 185256.0 58 168652.0 59 146894.0 60 120195.5 61 93020.0 62 66659.0 63 45682.5 64 31330.5 65 21155.5 66 13787.5 67 9731.0 68 6366.0 69 3267.0 70 1471.5 71 681.0 72 322.5 73 194.0 74 117.5 75 74.5 76 59.0 77 50.5 78 33.5 79 23.0 80 21.5 81 20.5 82 10.0 83 2.5 84 2.5 85 5.5 86 4.0 87 1.0 88 1.0 89 1.5 90 1.5 91 1.0 92 1.5 93 1.0 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.16278200528120557 2 0.0 3 0.0 4 9.127140740620187E-4 5 6.927829718783997E-4 6 9.896899598262852E-5 7 1.869414368560761E-4 8 0.0 9 2.0893454707443797E-4 10-11 2.254293797382094E-4 12-13 0.0 14-15 0.0 16-17 1.649483266377142E-5 18-19 0.0 20-21 5.4982775545904735E-6 22-23 6.103088085595425E-4 24-25 0.0 26-27 9.896899598262852E-5 28-29 0.005426799946380797 30-31 0.0038487942882133312 32-33 1.3745693886476183E-4 34-35 9.072157965074281E-4 36-37 5.388312003498664E-4 38-39 0.001220617617119085 40-41 0.00301855437747017 42-43 0.0011931262293461327 44-45 7.642605800880759E-4 46-47 0.008929202748654929 48-49 0.0088302337526723 50-51 0.008027485229702092 52-53 0.014817858009621326 54-55 0.0060591018651587015 56-57 8.027485229702091E-4 58-59 0.0012810986702195804 60-61 5.4982775545904735E-6 62-63 1.429552164193523E-4 64-65 9.237106291711995E-4 66-67 0.0011546382864639996 68-69 0.002892093993714589 70-71 0.0020233661400892943 72-73 0.0056907172690011405 74-75 0.002149826523844875 76-77 0.0014680401070756564 78-79 0.001556012547949104 80-81 2.474224899565713E-4 82-83 0.0022377989647183226 84-85 6.0481053100495205E-5 86-87 0.0019683833645433896 88-89 2.0893454707443797E-4 90-91 0.0010281779027084186 92-93 0.0016329884337133707 94-95 9.072157965074281E-4 96-97 0.001116150343581866 98-99 0.5308367047854918 100-101 1.8144315930148562E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 9093757.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 74.10026320579152 #Duplication Level Percentage of deduplicated Percentage of total 1 89.37472216872003 66.22690436646644 2 6.306746334288424 9.34663126685866 3 1.5680705118367215 3.4858331295702394 4 0.7561296880366325 2.2411763560491 5 0.42434394538748454 1.5721999021498314 6 0.25924360408341673 1.152601157819951 7 0.19276212673505164 0.9998607029022827 8 0.14947985910112138 0.886119752268618 9 0.11778060712559132 0.7854816589689817 >10 0.8399179551912711 11.29758441002433 >50 0.009673098087089275 0.43133554212499425 >100 8.714687050365865E-4 0.11212899540615186 >500 4.310545035788766E-5 0.028363831928058067 >1k 4.310545035788549E-5 0.1311507814150907 >5k 8.621090071577097E-5 0.48825398068949744 >10k+ 8.621090071577097E-5 0.8143741653578063 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15345 0.16874213815038164 No Hit AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 10151 0.11162603091329579 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 2.1993110218361894E-5 0.0 3 1.0996555109180947E-5 0.0 0.0 3.298966532754284E-5 0.0 4 1.0996555109180947E-5 0.0 0.0 3.298966532754284E-5 0.0 5 1.0996555109180947E-5 0.0 0.0 3.298966532754284E-5 0.0 6 1.0996555109180947E-5 0.0 0.0 3.298966532754284E-5 0.0 7 1.0996555109180947E-5 0.0 0.0 3.298966532754284E-5 0.0 8 1.0996555109180947E-5 0.0 0.0 3.298966532754284E-5 0.0 9 1.0996555109180947E-5 0.0 0.0 3.298966532754284E-5 0.0 10-11 1.0996555109180947E-5 0.0 0.0 3.298966532754284E-5 0.0 12-13 1.0996555109180947E-5 0.0 0.0 3.298966532754284E-5 0.0 14-15 1.0996555109180947E-5 0.0 0.0 3.298966532754284E-5 0.0 16-17 1.0996555109180947E-5 0.0 0.0 3.298966532754284E-5 0.0 18-19 1.0996555109180947E-5 0.0 0.0 3.298966532754284E-5 0.0 20-21 1.0996555109180947E-5 0.0 0.0 3.848794288213332E-5 0.0 22-23 1.0996555109180947E-5 0.0 0.0 4.398622043672379E-5 0.0 24-25 1.0996555109180947E-5 0.0 0.0 4.398622043672379E-5 0.0 26-27 1.0996555109180947E-5 0.0 0.0 4.398622043672379E-5 0.0 28-29 1.0996555109180947E-5 0.0 0.0 5.4982775545904735E-5 0.0 30-31 1.0996555109180947E-5 0.0 0.0 7.147760820967615E-5 0.0 32-33 1.0996555109180947E-5 0.0 0.0 7.697588576426662E-5 0.0 34-35 2.1993110218361894E-5 0.0 0.0 8.24741633188571E-5 0.0 36-37 2.1993110218361894E-5 0.0 0.0 9.347071842803805E-5 0.0 38-39 2.7491387772952367E-5 0.0 0.0 1.0446727353721899E-4 0.0 40-41 3.848794288213332E-5 0.0 0.0 1.0996555109180947E-4 0.0 42-43 5.4982775545904735E-5 0.0 0.0 1.0996555109180947E-4 0.0 44-45 5.4982775545904735E-5 0.0 0.0 1.0996555109180947E-4 0.0 46-47 6.048105310049521E-5 0.0 0.0 1.0996555109180947E-4 0.0 48-49 6.597933065508568E-5 0.0 0.0 1.2096210620099041E-4 0.0 50-51 8.24741633188571E-5 0.0 0.0 1.2096210620099041E-4 0.0 52-53 1.264603837555809E-4 0.0 0.0 1.2096210620099041E-4 0.0 54-55 1.3195866131017136E-4 0.0 0.0 1.2096210620099041E-4 0.0 56-57 1.5945004908312374E-4 0.0 0.0 1.2096210620099041E-4 0.0 58-59 2.3642593484739038E-4 0.0 0.0 1.2096210620099041E-4 0.0 60-61 2.859104328387046E-4 0.0 0.0 1.2096210620099041E-4 0.0 62-63 3.518897634937903E-4 0.0 0.0 1.264603837555809E-4 0.0 64-65 4.0137426148510453E-4 0.0 0.0 1.3195866131017136E-4 0.0 66-67 5.058415350223236E-4 0.0 0.0 1.5395177152853324E-4 0.0 68-69 6.323019187779045E-4 0.0 0.0 1.7044660419230468E-4 0.0 70-71 7.422674698697139E-4 0.0 0.0 1.9243971441066656E-4 0.0 72-73 8.522330209615234E-4 0.0 0.0 2.08934547074438E-4 0.0 74-75 0.0010171813475992376 0.0 0.0 2.1993110218361894E-4 0.0 76-77 0.0012151193395644948 0.0 0.0 2.3092765729279988E-4 0.0 78-79 0.0014900332172940183 0.0 0.0 2.3092765729279988E-4 0.0 80-81 0.0018199298705694467 0.0 0.0 2.3092765729279988E-4 0.0 82-83 0.002254293797382094 0.0 0.0 2.3092765729279988E-4 0.0 84-85 0.0027106508344131035 0.0 0.0 2.3092765729279988E-4 0.0 86-87 0.0034804096920557697 0.0 0.0 2.3642593484739035E-4 1.0996555109180947E-5 88-89 0.004486594484545826 0.0 0.0 2.4192421240198082E-4 1.0996555109180947E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTCACG 350 0.0 23.059752 3 ACTCGCG 185 0.0 21.817596 50-51 TCAACGC 8540 0.0 19.484835 10-11 CTCGCGT 195 0.0 19.479942 52-53 CAACGCA 8575 0.0 19.211529 12-13 ATCAACG 8570 0.0 19.111944 10-11 AACGCAG 9045 0.0 18.39696 12-13 AAGCAGT 9515 0.0 16.841776 1 AGTGGTA 10805 0.0 16.433142 4 GGTATCA 11080 0.0 16.025103 7 ACGCAGA 10370 0.0 15.817429 14-15 TGGTATC 11260 0.0 15.811091 6 CGCGTAG 245 2.910383E-11 15.503079 54-55 CGCAGAG 10930 0.0 15.289353 14-15 CGTACGC 280 1.8189894E-12 15.260299 20-21 TATCAAC 11770 0.0 15.085653 9 GTATCAA 12195 0.0 14.520983 8 AGCAGTG 12830 0.0 14.454556 1 CGTAGCG 300 3.6379788E-12 14.242749 56-57 GTGGTAT 12680 0.0 14.115488 5 >>END_MODULE