FastQCFastQC Report
Sat 18 Jun 2016
SRR578596_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR578596_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8266431
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT96192111.636472862351358No Hit
AAGCAGTGGTATCAACGCAGAGTATTTTTTTTTTTTTTTTTTTTTTTTTT222740.26945122992014325No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTG97090.1174509289438211No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT2115550.091.68841
AGTGGTA2123750.090.978735
TGGTATC2131900.090.629777
GGTATCA2136950.090.522228
GTATCAA2139100.090.498359
AGCAGTG2144900.090.376162
GTGGTAT2142650.090.2603766
GCAGTGG2153350.089.766183
CAGTGGT2156600.089.497114
CAACGCA2121550.046.10452312-13
ATCAACG2124450.046.09409310-11
ACGCAGA2129050.045.9182914-15
TCAACGC2123400.045.81175212-13
AACGCAG2128200.045.68684414-15
CGCAGAG2135000.045.4956616-17
TATCAAC2144650.045.3644710-11
AGAGTAC2100950.045.3630318-19
GCAGAGT2157600.045.14999416-17
GAGTACT1214100.044.9930220-21
CAGAGTA2138700.044.98728618-19
Produced by FastQC (version 0.11.5)