##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR578596_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8266431
Sequences flagged as poor quality	0
Sequence length	101
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.14035549319894	31.0	31.0	34.0	28.0	34.0
2	31.494469862507774	33.0	31.0	34.0	28.0	34.0
3	31.616425153733214	34.0	31.0	34.0	28.0	34.0
4	35.00963196813716	37.0	35.0	37.0	32.0	37.0
5	35.0523658880114	37.0	35.0	37.0	32.0	37.0
6	35.08792415977343	37.0	35.0	37.0	32.0	37.0
7	35.03417049026357	37.0	35.0	37.0	32.0	37.0
8	35.06900704790254	37.0	35.0	37.0	32.0	37.0
9	36.58530095999108	39.0	37.0	39.0	32.0	39.0
10-11	36.49060822016176	39.0	37.0	39.0	32.0	39.0
12-13	36.534261279142115	39.0	37.0	39.0	32.0	39.0
14-15	37.810108739793506	40.0	38.0	41.0	32.0	41.0
16-17	37.78041388115379	40.0	38.0	41.0	32.0	41.0
18-19	37.72000480013684	40.0	38.0	41.0	32.0	41.0
20-21	37.65455539397837	40.0	38.0	41.0	32.0	41.0
22-23	37.39593235581353	39.5	37.5	41.0	32.0	41.0
24-25	37.36213971906376	40.0	37.0	41.0	32.0	41.0
26-27	37.25247389109036	40.0	37.0	41.0	31.0	41.0
28-29	37.090797951377084	40.0	37.0	41.0	30.5	41.0
30-31	36.85686628243797	40.0	36.5	41.0	30.0	41.0
32-33	36.608346939567994	40.0	36.0	41.0	30.0	41.0
34-35	36.44319894522799	39.5	36.0	41.0	30.0	41.0
36-37	36.17241219336374	39.0	36.0	40.5	29.0	41.0
38-39	35.34797707741104	38.0	35.0	40.0	27.5	41.0
40-41	35.13278880571313	38.0	35.0	40.0	27.0	41.0
42-43	34.849066906867066	38.0	34.5	40.0	25.5	41.0
44-45	34.5773417814774	37.5	34.0	40.0	24.5	41.0
46-47	34.45933287291698	37.5	34.0	40.0	24.0	41.0
48-49	34.5177908096009	38.0	34.0	40.0	24.0	41.0
50-51	33.66153567361779	37.5	33.5	39.5	22.0	40.5
52-53	33.260061264649764	37.5	33.0	39.5	16.0	40.5
54-55	32.90576162796254	38.0	33.0	40.0	4.5	41.0
56-57	32.42084649348673	38.0	32.5	41.0	2.0	41.0
58-59	32.1402608937279	38.0	32.0	40.0	2.0	41.0
60-61	31.882065428236203	37.5	31.5	40.0	2.0	41.0
62-63	31.57183728987758	37.0	31.0	40.0	2.0	41.0
64-65	31.298412761202506	36.5	31.0	40.0	2.0	41.0
66-67	30.967949590337113	36.0	31.0	39.5	2.0	41.0
68-69	30.63708479511896	35.5	30.5	39.0	2.0	41.0
70-71	30.34338283353481	35.0	30.0	39.0	2.0	41.0
72-73	30.01691588546496	35.0	30.0	38.0	2.0	40.0
74-75	29.647670016237964	35.0	29.5	37.0	2.0	39.5
76-77	29.274923603668864	35.0	29.0	37.0	2.0	39.0
78-79	28.926694966666993	35.0	29.0	36.0	2.0	39.0
80-81	28.588200820886307	35.0	29.0	36.0	2.0	38.5
82-83	28.304034897285177	35.0	28.5	35.5	2.0	37.0
84-85	28.017402492079107	34.5	28.0	35.0	2.0	37.0
86-87	27.76098227880932	34.0	27.0	35.0	2.0	36.5
88-89	27.493445780893836	34.0	27.0	35.0	2.0	36.0
90-91	27.25105120940343	34.0	26.0	35.0	2.0	36.0
92-93	26.966764133154925	34.0	25.5	35.0	2.0	35.5
94-95	26.68537994208141	34.0	25.0	35.0	2.0	35.0
96-97	26.41497806006002	34.0	24.5	35.0	2.0	35.0
98-99	26.102501732609877	34.0	23.5	35.0	2.0	35.0
100-101	25.24872910691446	33.0	18.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.5971550458229906
1101	2	0.6196578735347877
1101	3	0.5500738294352914
1101	4	0.5946599772433672
1101	5	0.5626056507205064
1101	6	0.5355430585968435
1101	7	0.5015429417244945
1101	8	0.5127904446350087
1101	9	0.6608637623631353
1101	10-11	0.6730508907159347
1101	12-13	0.6883711488512176
1101	14-15	0.8388167559633359
1101	16-17	0.795762517503313
1101	18-19	0.8107793209018084
1101	20-21	0.8486425447271557
1101	22-23	0.8483703088356336
1101	24-25	0.8159066689054697
1101	26-27	0.9068222435522628
1101	28-29	1.0277940958486198
1101	30-31	1.0831066307230373
1101	32-33	1.060564640192048
1101	34-35	1.1309675681786402
1101	36-37	1.1045370175245992
1101	38-39	1.13102858032655
1101	40-41	1.0593791926555483
1101	42-43	1.1271287007872886
1101	44-45	1.2300010352208304
1101	46-47	1.1656008554633672
1101	48-49	1.1031372339568364
1101	50-51	1.2198845365954867
1101	52-53	0.9571963877955127
1101	54-55	0.8455627299842305
1101	56-57	0.8426652966765076
1101	58-59	0.8344758571422162
1101	60-61	0.785566595775915
1101	62-63	0.7956605566703665
1101	64-65	0.8339732265643676
1101	66-67	0.8137389170341081
1101	68-69	0.7562985531260473
1101	70-71	0.8344073174889033
1101	72-73	0.7879768135877256
1101	74-75	0.8108323339709429
1101	76-77	0.7543459182755861
1101	78-79	0.7586841021993358
1101	80-81	0.7413733660182835
1101	82-83	0.8368061474451842
1101	84-85	0.7730645442038906
1101	86-87	0.7730265544362425
1101	88-89	0.7621971788540804
1101	90-91	0.7562404123546749
1101	92-93	0.8946701480990704
1101	94-95	0.9638249744481193
1101	96-97	0.9849406714174798
1101	98-99	0.9630858625057783
1101	100-101	1.147734712302526
1102	1	0.4558627296104376
1102	2	0.3872092943219485
1102	3	0.48483149481906906
1102	4	0.4576688649493619
1102	5	0.48580621913102817
1102	6	0.48359717356428433
1102	7	0.49347747786190865
1102	8	0.40635534966313713
1102	9	0.5637736553653525
1102	10-11	0.5255255222307653
1102	12-13	0.5680143976040526
1102	14-15	0.6951859564161182
1102	16-17	0.7112782078699169
1102	18-19	0.8086069471659627
1102	20-21	0.8265604432753761
1102	22-23	0.8414748726147252
1102	24-25	0.862530025660476
1102	26-27	0.9550931691120894
1102	28-29	0.9656805403353914
1102	30-31	0.9706317872153178
1102	32-33	1.0535055872799859
1102	34-35	1.0035138362098976
1102	36-37	1.0149303436331891
1102	38-39	1.0785828673065367
1102	40-41	1.0934047170967958
1102	42-43	1.0874717017531452
1102	44-45	1.174010679380693
1102	46-47	1.2198621558186176
1102	48-49	1.1785181608749653
1102	50-51	1.16315017418502
1102	52-53	0.9620425792408938
1102	54-55	0.9390398900235226
1102	56-57	0.8507906209579303
1102	58-59	0.9099764110789366
1102	60-61	0.9161386814388557
1102	62-63	0.9516326334852145
1102	64-65	0.9098820295532306
1102	66-67	0.8704476227911542
1102	68-69	0.821128016663156
1102	70-71	0.7704523054580186
1102	72-73	0.8127657647329727
1102	74-75	0.7624331193999865
1102	76-77	0.8531667580140976
1102	78-79	0.9255304318153641
1102	80-81	0.8787756702208824
1102	82-83	0.9095716351136964
1102	84-85	0.9460893406050346
1102	86-87	0.918150324219333
1102	88-89	0.9211485790217111
1102	90-91	0.9203328276356153
1102	92-93	0.887952602476112
1102	94-95	1.0320815101483838
1102	96-97	1.0839092015752598
1102	98-99	1.1663769570863565
1102	100-101	1.1918354513677052
1103	1	0.37249517712728064
1103	2	0.4101995125231035
1103	3	0.30377615651659085
1103	4	0.4031175822938451
1103	5	0.4378847003771327
1103	6	0.4702951497500649
1103	7	0.3223338860605196
1103	8	0.3422823434510036
1103	9	0.44651288329209393
1103	10-11	0.47417440216842266
1103	12-13	0.5359941782614897
1103	14-15	0.5966759942660715
1103	16-17	0.689959692528916
1103	18-19	0.6695935322918771
1103	20-21	0.6603992088054795
1103	22-23	0.7818496059938767
1103	24-25	0.6726249811860043
1103	26-27	0.7192764414868549
1103	28-29	0.7464089919276518
1103	30-31	0.8027169548904283
1103	32-33	0.8488717774761554
1103	34-35	0.8124132942848874
1103	36-37	0.8528994784662274
1103	38-39	0.7451781842431302
1103	40-41	0.8376423026280762
1103	42-43	0.7547037577112761
1103	44-45	0.7449270337556797
1103	46-47	0.9712849791820446
1103	48-49	0.979611498425065
1103	50-51	0.9131430802682416
1103	52-53	0.747960697758991
1103	54-55	0.6217712111576148
1103	56-57	0.653258569858302
1103	58-59	0.6832823179502228
1103	60-61	0.6980371418736944
1103	62-63	0.5433160430327852
1103	64-65	0.46646693417976337
1103	66-67	0.4501905981131813
1103	68-69	0.6327978554579445
1103	70-71	0.5299986065117146
1103	72-73	0.6179128782816008
1103	74-75	0.6070510239825033
1103	76-77	0.6334710481247576
1103	78-79	0.6063507616280539
1103	80-81	0.6810095498630453
1103	82-83	0.7044506415766101
1103	84-85	0.717961903219404
1103	86-87	0.739173657258938
1103	88-89	0.7298633815431543
1103	90-91	0.6665003109763248
1103	92-93	0.6719606745285311
1103	94-95	0.5495751839081358
1103	96-97	0.5737372166869221
1103	98-99	0.6302245237225463
1103	100-101	0.7027231494684223
1104	1	0.37257658976251307
1104	2	0.4007673483691825
1104	3	0.4366823392899235
1104	4	0.3854141739287016
1104	5	0.4564555462083959
1104	6	0.47378405028101156
1104	7	0.39784957314011393
1104	8	0.3562552005036892
1104	9	0.5305540664434645
1104	10-11	0.5314223867206138
1104	12-13	0.5228668015734641
1104	14-15	0.6454128160092694
1104	16-17	0.6617044379347092
1104	18-19	0.7030686928143908
1104	20-21	0.7246737929345599
1104	22-23	0.6841273123655398
1104	24-25	0.7286526683215158
1104	26-27	0.7367048006353016
1104	28-29	0.7161837760756242
1104	30-31	0.8401835425159874
1104	32-33	0.7943589519194774
1104	34-35	0.8880377186404687
1104	36-37	0.8452861555497861
1104	38-39	0.8924005793120173
1104	40-41	0.9046869161723095
1104	42-43	0.991468041480438
1104	44-45	0.9684919436981048
1104	46-47	1.1126194178418842
1104	48-49	1.001622940650897
1104	50-51	0.9386821741309745
1104	52-53	0.8642504397595161
1104	54-55	0.8957258029648045
1104	56-57	0.8530986551025634
1104	58-59	0.8072943208683157
1104	60-61	0.7741127926562363
1104	62-63	0.7210638495715038
1104	64-65	0.6359485109189578
1104	66-67	0.7295728649677571
1104	68-69	0.7134742122428896
1104	70-71	0.6320267594782578
1104	72-73	0.6624175848775558
1104	74-75	0.7673736086325746
1104	76-77	0.7371391999345285
1104	78-79	0.678803308529158
1104	80-81	0.8030541786475247
1104	82-83	0.7721946261597274
1104	84-85	0.7530933696452635
1104	86-87	0.7382517414994183
1104	88-89	0.7369612905892353
1104	90-91	0.7573707808791106
1104	92-93	0.6100505211886365
1104	94-95	0.49662275480296003
1104	96-97	0.6087340708777198
1104	98-99	0.8679165963205229
1104	100-101	0.8273967573595691
1105	1	0.1926435737440535
1105	2	0.13926330557409372
1105	3	0.1710193718019717
1105	4	0.1857735217080645
1105	5	0.1561554475070679
1105	6	0.10099008859635461
1105	7	0.13287871689902175
1105	8	0.11203750044260374
1105	9	0.07002993128203627
1105	10-11	0.13621914046503747
1105	12-13	0.13178076526607896
1105	14-15	0.021422613318030415
1105	16-17	0.009137920592685589
1105	18-19	-0.10293899047595545
1105	20-21	0.04927453333592524
1105	22-23	-0.015501921327533807
1105	24-25	-0.046285572645473394
1105	26-27	-0.3646127136184134
1105	28-29	-0.2136354788848891
1105	30-31	-0.35304523528441223
1105	32-33	-0.28424525504787823
1105	34-35	-0.17189765532160806
1105	36-37	-0.12958744523869115
1105	38-39	-0.135450517432254
1105	40-41	-0.06527942214982119
1105	42-43	0.032512910991066235
1105	44-45	0.028691764395411212
1105	46-47	0.04075341479317984
1105	48-49	0.02707252307923369
1105	50-51	0.0016708022673554979
1105	52-53	-0.07547857701688088
1105	54-55	-0.13716399304302485
1105	56-57	0.01166755301314737
1105	58-59	-0.009376923814819804
1105	60-61	-0.07477934503353012
1105	62-63	-0.016089635235847055
1105	64-65	-0.10382363466126066
1105	66-67	-0.07554327170954167
1105	68-69	-0.045757083996843306
1105	70-71	-0.023024153721134866
1105	72-73	-0.05184132793721119
1105	74-75	-0.04857929400662542
1105	76-77	-0.02375488844982243
1105	78-79	0.005887101431714115
1105	80-81	0.061677676916715996
1105	82-83	0.07191953862946932
1105	84-85	0.02284798796241816
1105	86-87	0.068819228178981
1105	88-89	0.2045423055726019
1105	90-91	0.22695228300585768
1105	92-93	0.23418212979403563
1105	94-95	0.272633135411116
1105	96-97	0.2458381519938051
1105	98-99	0.28594259804001965
1105	100-101	0.2960460940244225
1106	1	-0.13957806778187987
1106	2	-0.08779398182274534
1106	3	-0.1418809670513923
1106	4	-0.12586229404497118
1106	5	-0.20548566282977987
1106	6	-0.18431189055323216
1106	7	-0.24995709634904983
1106	8	-0.2705704749085456
1106	9	-0.20065777253097394
1106	10-11	-0.38529968491160815
1106	12-13	-0.4131375301967495
1106	14-15	-0.3806661426938831
1106	16-17	-0.3770148640168074
1106	18-19	-0.3820088886605433
1106	20-21	-0.19354151346591664
1106	22-23	-0.19786310075585334
1106	24-25	-0.27796992777655305
1106	26-27	-0.29694767631721675
1106	28-29	-0.3671523350179555
1106	30-31	-0.34806821263953935
1106	32-33	-0.29389450594965894
1106	34-35	-0.2996166945311387
1106	36-37	-0.379201951145852
1106	38-39	-0.3701314646731575
1106	40-41	-0.42700222848419145
1106	42-43	-0.46337481992110696
1106	44-45	-0.42709942860526695
1106	46-47	-0.4246569695366773
1106	48-49	-0.594652107893971
1106	50-51	-0.678022533161041
1106	52-53	-0.6582908492928468
1106	54-55	-0.6258117364116984
1106	56-57	-0.6560241422328836
1106	58-59	-0.6163731897099609
1106	60-61	-0.7706570987864225
1106	62-63	-0.6301400373964938
1106	64-65	-0.5882202969100803
1106	66-67	-0.7317143084004378
1106	68-69	-0.8350156543908973
1106	70-71	-0.6864650809678601
1106	72-73	-0.7690834817055929
1106	74-75	-0.8068398837435566
1106	76-77	-0.8484661059080558
1106	78-79	-0.7330130882624069
1106	80-81	-0.7146763701948338
1106	82-83	-0.8412875587316684
1106	84-85	-0.8980041895348023
1106	86-87	-0.8615558418461582
1106	88-89	-0.9245532684969149
1106	90-91	-0.8258241568932156
1106	92-93	-0.8609463264875039
1106	94-95	-0.8850767179801515
1106	96-97	-0.830523822760707
1106	98-99	-0.8774285602555096
1106	100-101	-1.038920091802943
1107	1	-0.40577626849444215
1107	2	-0.4522517868678193
1107	3	-0.3684786392277708
1107	4	-0.28310648650396786
1107	5	-0.3919451894907908
1107	6	-0.35966799498559965
1107	7	-0.3198343628406022
1107	8	-0.25517754566534023
1107	9	-0.31461412591592364
1107	10-11	-0.3340187966940462
1107	12-13	-0.4432574932171818
1107	14-15	-0.6147966339245343
1107	16-17	-0.4295102576913692
1107	18-19	-0.4732294300459259
1107	20-21	-0.43129844454431776
1107	22-23	-0.47489189510928753
1107	24-25	-0.4389080657142159
1107	26-27	-0.34595721795798795
1107	28-29	-0.4186868229466114
1107	30-31	-0.4317325525854443
1107	32-33	-0.3952314198385878
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2308	46-47	-2.7869575477574386
2308	48-49	-2.541323253694266
2308	50-51	-2.5058552303070982
2308	52-53	-2.4945713910832517
2308	54-55	-2.1464565015859414
2308	56-57	-2.1251179839968017
2308	58-59	-2.0514184310855796
2308	60-61	-1.9745281146375966
2308	62-63	-2.081965630488334
2308	64-65	-2.08491724232109
2308	66-67	-2.2405795979236096
2308	68-69	-2.0998011938979992
2308	70-71	-2.027065529687796
2308	72-73	-2.0077178637061053
2308	74-75	-1.8737406369002017
2308	76-77	-1.7614427712006524
2308	78-79	-1.8523676312848032
2308	80-81	-2.4539235077547907
2308	82-83	-2.265512680487607
2308	84-85	-2.0249600863243487
2308	86-87	-1.857876297172865
2308	88-89	-1.7633697090160716
2308	90-91	-1.9610801883605689
2308	92-93	-1.99281062941807
2308	94-95	-2.0872367004625154
2308	96-97	-2.072109579317118
2308	98-99	-2.1104222472744674
2308	100-101	-2.327040104798332
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	62434.0
3	11114.0
4	9783.0
5	9601.0
6	11249.0
7	13807.0
8	16706.0
9	19627.0
10	22605.0
11	27031.0
12	26637.0
13	30131.0
14	33781.0
15	39767.0
16	50137.0
17	68260.0
18	107933.0
19	179535.0
20	260791.0
21	186989.0
22	100066.0
23	84147.0
24	85520.0
25	90230.0
26	98546.0
27	110688.0
28	124577.0
29	143833.0
30	170275.0
31	203835.0
32	251689.0
33	318006.0
34	419150.0
35	608282.0
36	997011.0
37	1433079.0
38	1487672.0
39	351367.0
40	540.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	11.490234951804137	47.56592738909626	23.20199900267728	17.741838656422324
2	16.940895383014084	48.08920991955371	19.51688878050458	15.453005916927626
3	42.009962409110145	21.536572419851787	19.96036539003575	16.493099781002314
4	16.596799412432407	20.60607916524346	21.05868921978567	41.73843220253846
5	16.534629777700506	45.99305413964463	21.785118075267324	15.687198007387543
6	41.79830909283586	21.24782793626	20.311511804752094	16.64235116615205
7	16.9458026295065	20.743588881273	46.61076205170316	15.699846437517346
8	41.325162402216	21.383064295151335	21.051878339487622	16.239894963145037
9	40.880052845272715	21.170387290034405	21.786516228628393	16.16304363606448
10-11	16.068986443128793	33.80704832448222	33.716063058276056	16.40790217411293
12-13	15.93683776231645	21.525275360810834	33.631284206476295	28.90660267039642
14-15	16.145371479343655	46.33793183256666	21.019918186303975	16.49677850178571
16-17	28.718211818343814	21.233362757957924	21.13712887199248	28.911296551705785
18-19	16.316121477760746	33.77306804769751	21.09951199348252	28.81129848105922
20-21	28.80764833695632	33.70783220726204	21.188913054247337	16.295606401534304
22-23	28.662626496260373	21.1794662017646	33.75383275949429	16.404074542480732
24-25	16.249043942793527	33.45446264368076	21.569485285111455	28.727008128414244
26-27	16.484690318420352	26.17227626580941	40.88596118600032	16.457072229769917
28-29	26.626121373851042	21.063041301010813	35.81618814003006	16.494649185108084
30-31	24.404411323940973	22.256328283739578	36.305079170575475	17.034181221743967
32-33	19.27177155471349	24.64800045775576	37.39394588973675	18.686282097794003
34-35	18.784984907895886	23.725125186859376	38.059726770674416	19.430163134570325
36-37	18.801726337841966	23.630871743995467	38.282037024458226	19.28536489370434
38-39	18.848884327354305	23.603013903325145	38.132652829151496	19.415448940169057
40-41	19.052824718781423	23.614345849249617	37.871798637530716	19.461030794438244
42-43	19.293877125147624	23.693195444778294	37.87041721923403	19.14251021084005
44-45	19.121077235677696	24.04718123004379	37.6949802879265	19.136761246352016
46-47	19.40891416836411	23.601576803100187	37.713331863921496	19.276177164614207
48-49	19.05067133593907	23.842329068174454	37.88040187308827	19.226597722798203
50-51	19.492370772213768	23.888932742847345	37.05542747175276	19.56326901318613
52-53	19.75255717667466	25.06092193119872	35.788487413000574	19.398033479126042
54-55	20.398668287090203	25.723969281731762	34.21970840129517	19.65765402988286
56-57	20.422881022317778	26.541253066044877	32.95837446628108	20.077491445356266
58-59	20.607403895836175	26.92107798382375	32.3330227019312	20.138495418408873
60-61	20.767519274783947	26.79955811173422	32.34734006588844	20.085582547593393
62-63	20.617706492073616	26.741997465154967	32.21737779788214	20.422918244889285
64-65	20.227556972963114	26.471109558702103	32.582030487598466	20.719302980736316
66-67	20.484041683742582	26.02817494061458	33.09583807557742	20.391945300065426
68-69	20.36082853567004	26.121731341717812	33.029821355574306	20.487618767037834
70-71	20.07127155197735	26.181491126060724	33.56313913141536	20.184098190546568
72-73	19.87468863214696	25.935108801771356	33.4938067969663	20.696395769115387
74-75	20.12170713376884	26.124744815925304	32.85106697374548	20.90248107656037
76-77	20.71084019701906	26.517933505734803	32.35957413522526	20.41165216202088
78-79	20.444697164226	26.539671947674165	32.28070577147892	20.73492511662091
80-81	20.37145253951381	26.860790851632792	31.83190844364493	20.935848165208466
82-83	21.236841820008323	27.229176407495075	31.25704541591291	20.27693635658369
84-85	20.77538971516776	27.44951101953233	30.802287213980158	20.972812051319753
86-87	20.60400137328921	27.98633324089652	30.683166566548657	20.726498819265608
88-89	20.450685910905648	27.741151614270297	30.548182481139165	21.259979993684894
90-91	20.57446950517028	27.506984092029274	31.033643197719062	20.884903205081386
92-93	20.492413653152735	28.041548859587085	30.75970081671162	20.70633667054856
94-95	20.77679351251238	27.497380007874366	30.40897442491483	21.316852054698423
96-97	20.35541246369324	27.34164364148865	30.961372467008285	21.34157142780983
98-99	20.940389799238293	27.71751708852245	30.489319677254382	20.852773434984872
100-101	21.147331816263286	27.270334408501668	30.432476402551405	21.14985737268364
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	9346.0
1	5512.0
2	1266.5
3	738.5
4	603.5
5	559.0
6	657.0
7	831.5
8	815.0
9	869.5
10	1420.0
11	7079.5
12	126017.5
13	142824.5
14	43605.0
15	42686.5
16	46402.0
17	51211.0
18	57080.5
19	59917.0
20	61127.0
21	62290.5
22	63761.5
23	67995.0
24	72401.5
25	77517.5
26	84160.0
27	92691.5
28	101056.5
29	110644.5
30	121381.5
31	131530.5
32	143207.0
33	156719.5
34	175438.0
35	192689.0
36	204241.0
37	215363.5
38	225382.0
39	233366.0
40	240518.5
41	246060.0
42	249431.0
43	253551.5
44	260579.5
45	263634.0
46	262897.5
47	262797.0
48	262719.0
49	259861.0
50	254119.5
51	254764.0
52	254321.5
53	239881.0
54	215727.0
55	196132.0
56	186567.5
57	178984.5
58	193193.5
59	167118.0
60	108216.0
61	81518.0
62	58561.0
63	40698.5
64	28036.0
65	19016.5
66	12309.5
67	8050.0
68	5064.5
69	2992.0
70	1928.0
71	1462.0
72	1235.0
73	1035.0
74	876.0
75	771.5
76	672.0
77	577.0
78	498.0
79	415.5
80	364.0
81	313.0
82	257.0
83	220.5
84	162.5
85	122.0
86	103.0
87	85.5
88	83.0
89	63.0
90	40.5
91	30.5
92	22.5
93	14.0
94	11.5
95	8.0
96	3.0
97	2.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05215068026334461
2	0.0937889640644191
3	0.08447418238898988
4	0.05532012545680234
5	0.08616777905724973
6	0.001197614786840899
7	0.0010766435962509093
8	0.004596905242419613
9	0.009568821175668193
10-11	0.009762375080612177
12-13	0.01652466463459261
14-15	0.011425728951224536
16-17	0.0013548773346078857
18-19	5.504189171844536E-4
20-21	7.863127388349337E-5
22-23	3.689621312994689E-4
24-25	7.076814649514403E-4
26-27	0.0013246345369603884
28-29	0.0014516542870798776
30-31	0.004675536516303106
32-33	1.693596668259857E-4
34-35	0.002274258383091808
36-37	3.689621312994689E-4
38-39	0.002504103645212789
40-41	0.0031815423125167316
42-43	0.0011310806320164045
44-45	6.109045124794485E-4
46-47	0.0010282551200149134
48-49	0.0022924040616803068
50-51	0.004336817182651134
52-53	0.1329291927798103
54-55	0.14273390777712897
56-57	0.1292093286691681
58-59	0.12253171894860068
60-61	0.11056766819925068
62-63	0.1151161849654343
64-65	0.11769891988453057
66-67	0.12588866948747288
68-69	0.13860274161848082
70-71	0.1258342324517074
72-73	0.11755980301535209
74-75	0.12261639878201366
76-77	0.13412680756665118
78-79	0.14794171753202803
80-81	0.13797973998694238
82-83	0.11773521124170759
84-85	0.12427370409309653
86-87	0.12509025962957895
88-89	0.12143088111423177
90-91	0.1316650438381449
92-93	0.14871593315180395
94-95	0.1580670061844102
96-97	0.1590408242686596
98-99	0.1414697588354636
100-101	0.13094526525413444
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	8266431.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.53372109975629
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.66621051562444	51.256982584621035
2	5.832667303568001	6.594847732151619
3	1.3953019315527602	2.36644830745065
4	0.6214209539467336	1.4052495558387657
5	0.3486225981584609	0.9854466366681419
6	0.21561025560836375	0.7313550034086241
7	0.15373352890379952	0.6083789912710107
8	0.12100269059816451	0.5472585890077388
9	0.09615103608076313	0.489219827151822
>10	0.5237081302578308	4.589064600501337
>50	0.009524295911750528	0.4142868048152947
>100	0.011692434379194162	1.3230016478266573
>500	0.0017593233991111974	0.6655439395656996
>1k	0.0021991542462855124	2.228102657764051
>5k	3.0788159447997173E-4	1.3225342234552473
>10k+	8.796616985142049E-5	24.472278898502243
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	961921	11.636472862351358	No Hit
AAGCAGTGGTATCAACGCAGAGTATTTTTTTTTTTTTTTTTTTTTTTTTT	22274	0.26945122992014325	No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTG	9709	0.1174509289438211	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	1.209711905899898E-5	0.0	0.0	0.0	0.0
6	1.209711905899898E-5	0.0	0.0	0.0	0.0
7	1.209711905899898E-5	1.209711905899898E-5	0.0	0.0	0.0
8	1.209711905899898E-5	1.209711905899898E-5	0.0	0.0	0.0
9	1.209711905899898E-5	1.209711905899898E-5	0.0	0.0	0.0
10-11	1.209711905899898E-5	1.209711905899898E-5	0.0	0.0	0.0
12-13	1.209711905899898E-5	1.209711905899898E-5	0.0	0.0	0.0
14-15	1.209711905899898E-5	1.209711905899898E-5	0.0	6.04855952949949E-6	0.0
16-17	1.8145678588498473E-5	1.209711905899898E-5	0.0	1.209711905899898E-5	0.0
18-19	2.419423811799796E-5	2.419423811799796E-5	0.0	1.209711905899898E-5	0.0
20-21	2.419423811799796E-5	2.419423811799796E-5	0.0	1.209711905899898E-5	0.0
22-23	3.629135717699694E-5	2.419423811799796E-5	0.0	1.209711905899898E-5	0.0
24-25	3.629135717699694E-5	2.419423811799796E-5	0.0	1.209711905899898E-5	0.0
26-27	4.233991670649643E-5	2.419423811799796E-5	0.0	1.8145678588498473E-5	0.0
28-29	4.838847623599592E-5	2.419423811799796E-5	0.0	2.419423811799796E-5	0.0
30-31	4.838847623599592E-5	2.419423811799796E-5	0.0	2.419423811799796E-5	0.0
32-33	4.838847623599592E-5	2.419423811799796E-5	0.0	2.419423811799796E-5	0.0
34-35	4.838847623599592E-5	2.419423811799796E-5	0.0	2.419423811799796E-5	0.0
36-37	1.0887407153099082E-4	2.419423811799796E-5	0.0	2.419423811799796E-5	0.0
38-39	1.209711905899898E-4	2.419423811799796E-5	0.0	2.419423811799796E-5	0.0
40-41	1.209711905899898E-4	2.419423811799796E-5	0.0	3.024279764749745E-5	0.0
42-43	1.209711905899898E-4	2.419423811799796E-5	0.0	3.629135717699694E-5	0.0
44-45	1.209711905899898E-4	2.419423811799796E-5	0.0	3.629135717699694E-5	0.0
46-47	1.4516542870798775E-4	2.419423811799796E-5	0.0	4.233991670649643E-5	0.0
48-49	1.6331110729648623E-4	2.419423811799796E-5	0.0	4.838847623599592E-5	0.0
50-51	1.814567858849847E-4	2.419423811799796E-5	0.0	5.443703576549541E-5	0.0
52-53	2.1169958353248213E-4	2.419423811799796E-5	0.0	7.258271435399388E-5	6.04855952949949E-6
54-55	2.7823373835697654E-4	2.419423811799796E-5	0.0	7.258271435399388E-5	1.209711905899898E-5
56-57	3.2057365506347297E-4	2.419423811799796E-5	0.0	7.258271435399388E-5	1.209711905899898E-5
58-59	4.4154484565346277E-4	2.419423811799796E-5	0.0	7.258271435399388E-5	1.209711905899898E-5
60-61	5.201761195369561E-4	2.419423811799796E-5	0.0	7.258271435399388E-5	1.209711905899898E-5
62-63	5.564674767139531E-4	2.419423811799796E-5	0.0	7.863127388349337E-5	1.209711905899898E-5
64-65	6.04855952949949E-4	2.419423811799796E-5	0.0	8.467983341299287E-5	1.209711905899898E-5
66-67	6.713901077744435E-4	2.419423811799796E-5	0.0	8.467983341299287E-5	1.209711905899898E-5
68-69	7.984098578939327E-4	2.419423811799796E-5	0.0	1.0282551200149133E-4	3.024279764749745E-5
70-71	9.254296080134219E-4	2.419423811799796E-5	0.0	1.0887407153099082E-4	3.629135717699694E-5
72-73	0.0011008378343689072	2.419423811799796E-5	0.0	1.209711905899898E-4	3.629135717699694E-5
74-75	0.0012883431797833915	2.419423811799796E-5	0.0	1.209711905899898E-4	3.629135717699694E-5
76-77	0.0015786740371993668	2.419423811799796E-5	0.0	1.209711905899898E-4	3.629135717699694E-5
78-79	0.00177827650167285	2.419423811799796E-5	0.0	1.330683096489888E-4	3.629135717699694E-5
80-81	0.0020323160019118285	2.419423811799796E-5	0.0	1.4516542870798775E-4	5.443703576549541E-5
82-83	0.002407326692740797	2.419423811799796E-5	0.0	1.5726254776698674E-4	6.04855952949949E-5
84-85	0.003096862479103739	2.419423811799796E-5	0.0	1.5726254776698674E-4	6.04855952949949E-5
86-87	0.0037924468249961804	2.419423811799796E-5	0.0	1.5726254776698674E-4	6.04855952949949E-5
88-89	0.004814653385481594	2.419423811799796E-5	0.0	1.6935966682598573E-4	6.04855952949949E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGCAGT	211555	0.0	91.6884	1
AGTGGTA	212375	0.0	90.97873	5
TGGTATC	213190	0.0	90.62977	7
GGTATCA	213695	0.0	90.52222	8
GTATCAA	213910	0.0	90.49835	9
AGCAGTG	214490	0.0	90.37616	2
GTGGTAT	214265	0.0	90.260376	6
GCAGTGG	215335	0.0	89.76618	3
CAGTGGT	215660	0.0	89.49711	4
CAACGCA	212155	0.0	46.104523	12-13
ATCAACG	212445	0.0	46.094093	10-11
ACGCAGA	212905	0.0	45.91829	14-15
TCAACGC	212340	0.0	45.811752	12-13
AACGCAG	212820	0.0	45.686844	14-15
CGCAGAG	213500	0.0	45.49566	16-17
TATCAAC	214465	0.0	45.36447	10-11
AGAGTAC	210095	0.0	45.36303	18-19
GCAGAGT	215760	0.0	45.149994	16-17
GAGTACT	121410	0.0	44.99302	20-21
CAGAGTA	213870	0.0	44.987286	18-19
>>END_MODULE