FastQCFastQC Report
Sat 18 Jun 2016
SRR578596_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR578596_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8266431
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT138520.16756929320525388No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT101180.12239865063895167No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT82910.10029721411816055No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCACG2801.6134436E-920.3225773
CTCGCGT1301.5277692E-820.0566952-53
TCAACGC81800.019.18291710-11
CAACGCA81350.019.11420412-13
ATCAACG81950.019.03210810-11
AACGCAG84900.018.39872412-13
AGTGGTA98050.017.6994654
ACTCGCG1507.87295E-817.38246550-51
AAGCAGT87850.017.0597171
GGTATCA105750.016.27597
ACGCAGA96550.016.1050314-15
TGGTATC106350.016.0949086
TATCAAC111700.015.5777739
GTATCAA112550.015.4189338
CGCAGAG102800.015.21811214-15
AGCAGTG118900.015.037861
GTGGTAT117600.014.9182695
CGTACGC2001.3205499E-714.22360920-21
CGCGTAG1858.5144165E-714.09389154-55
AGAGTAC108500.013.87310518-19
Produced by FastQC (version 0.11.5)