##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR578596_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 8266431 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.023682771924186 31.0 31.0 34.0 30.0 34.0 2 32.28933453869028 34.0 31.0 34.0 30.0 34.0 3 32.36116166698785 34.0 31.0 34.0 30.0 34.0 4 35.92535811403978 37.0 35.0 37.0 35.0 37.0 5 35.80768157382551 37.0 35.0 37.0 35.0 37.0 6 35.785844701298544 37.0 35.0 37.0 33.0 37.0 7 35.78191241177722 37.0 35.0 37.0 33.0 37.0 8 35.70144431133581 37.0 35.0 37.0 33.0 37.0 9 37.34767374215063 39.0 37.0 39.0 34.0 39.0 10-11 37.345646567424325 39.0 37.0 39.0 34.0 39.0 12-13 37.340312645203234 39.0 37.0 39.0 34.0 39.0 14-15 38.666765258187 40.0 38.0 41.0 34.0 41.0 16-17 38.622320200821854 40.0 38.0 41.0 34.0 41.0 18-19 38.57507266436991 40.0 38.0 41.0 34.0 41.0 20-21 38.515618167017905 40.0 38.0 41.0 34.0 41.0 22-23 38.40217090059785 40.0 38.0 41.0 34.0 41.0 24-25 38.328163508532285 40.0 38.0 41.0 33.5 41.0 26-27 38.16837441696423 40.0 38.0 41.0 33.0 41.0 28-29 38.07941274777471 40.0 37.5 41.0 33.0 41.0 30-31 37.9830794571442 40.0 37.0 41.0 33.0 41.0 32-33 37.857276919144425 40.0 37.0 41.0 33.0 41.0 34-35 37.727188432347646 40.0 37.0 41.0 32.0 41.0 36-37 37.603255201670464 40.0 37.0 41.0 32.0 41.0 38-39 37.47979575466123 40.0 37.0 41.0 31.5 41.0 40-41 37.34154618843368 40.0 36.0 41.0 31.0 41.0 42-43 37.19101574790862 39.5 36.0 41.0 31.0 41.0 44-45 37.045211228400746 39.0 36.0 41.0 31.0 41.0 46-47 37.12622986873053 39.0 36.0 41.0 31.0 41.0 48-49 37.107826279563696 39.0 36.0 41.0 31.0 41.0 50-51 37.08584593520469 39.0 36.0 41.0 31.0 41.0 52-53 37.100782973933974 39.5 36.0 41.0 31.0 41.0 54-55 36.98382675667407 39.0 36.0 41.0 31.0 41.0 56-57 36.848917084047514 39.0 35.5 41.0 31.0 41.0 58-59 36.64952184322351 39.0 35.0 41.0 30.5 41.0 60-61 36.442724738160884 39.0 35.0 41.0 30.0 41.0 62-63 36.19418446727494 38.5 35.0 40.5 30.0 41.0 64-65 35.90766771052707 38.0 35.0 40.0 29.0 41.0 66-67 35.61204315381087 37.5 34.5 40.0 29.0 41.0 68-69 35.2994837191528 37.0 34.0 40.0 29.0 41.0 70-71 34.950569961813024 36.5 34.0 39.0 28.5 41.0 72-73 34.54146559742651 36.0 34.0 39.0 28.0 41.0 74-75 34.161798725471726 35.5 34.0 39.0 28.0 40.0 76-77 32.715058167182434 34.5 31.5 36.5 26.0 39.0 78-79 33.3127828079615 35.0 33.0 37.0 26.5 39.0 80-81 33.24579210786372 35.0 33.0 37.0 27.0 39.0 82-83 32.953186145266315 35.0 33.0 36.0 26.5 38.5 84-85 32.651530327900886 35.0 33.0 36.0 26.0 37.0 86-87 32.355600076986065 35.0 33.0 35.5 26.0 37.0 88-89 32.09150393924537 35.0 33.0 35.0 26.0 36.5 90-91 31.840140987083785 35.0 32.0 35.0 25.0 36.0 92-93 31.597558910247965 35.0 32.0 35.0 25.0 36.0 94-95 31.34592873272637 35.0 32.0 35.0 24.5 36.0 96-97 30.74821141070433 35.0 32.0 35.0 20.5 35.5 98-99 30.729486280112905 34.5 32.0 35.0 21.5 35.0 100-101 30.16658815636397 34.0 31.5 35.0 17.5 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.46120299200855897 1101 2 0.4090405180321639 1101 3 0.4001483507527297 1101 4 0.2824958926045156 1101 5 0.3072193476471696 1101 6 0.3189903644432519 1101 7 0.3505713782535267 1101 8 0.3843891059588529 1101 9 0.4885747844365298 1101 10-11 0.4241535577805351 1101 12-13 0.43967033634342556 1101 14-15 0.5985224565564025 1101 16-17 0.5864968754337738 1101 18-19 0.5550602681796946 1101 20-21 0.6347855306832244 1101 22-23 0.6764994199747534 1101 24-25 0.7093255885741101 1101 26-27 0.7303911049444523 1101 28-29 0.7330748414819013 1101 30-31 0.6947708063288687 1101 32-33 0.6586291685924408 1101 34-35 0.678170706169567 1101 36-37 0.6929953493138612 1101 38-39 0.7545268101286027 1101 40-41 0.7639427154754728 1101 42-43 0.7880304127362763 1101 44-45 0.7412181179705044 1101 46-47 0.7829834221583098 1101 48-49 0.7505471461676834 1101 50-51 0.7164909735054223 1101 52-53 0.7054696039374377 1101 54-55 0.8135804116884913 1101 56-57 0.7889759154698339 1101 58-59 0.7761661874008183 1101 60-61 0.7372295451964206 1101 62-63 0.7993358877454781 1101 64-65 0.7803184587493845 1101 66-67 0.784254768441933 1101 68-69 0.8170195853010753 1101 70-71 0.7838518261306788 1101 72-73 0.7481707763549323 1101 74-75 0.7961463029050222 1101 76-77 0.7458293499762974 1101 78-79 0.6236957568484485 1101 80-81 0.5868448292932484 1101 82-83 0.6213382229332183 1101 84-85 0.571003576176146 1101 86-87 0.546267415106648 1101 88-89 0.5597708403700921 1101 90-91 0.5988210813618657 1101 92-93 0.6383221354393811 1101 94-95 0.626103780231059 1101 96-97 1.0368345487073434 1101 98-99 0.7810002108898928 1101 100-101 0.8065755967070807 1102 1 0.3829012484808061 1102 2 0.36782464371233203 1102 3 0.3425380548771031 1102 4 0.23359842008012066 1102 5 0.29616319491726983 1102 6 0.29418745722734485 1102 7 0.26255909305136527 1102 8 0.28341471566059795 1102 9 0.42402913135313725 1102 10-11 0.3633661257542471 1102 12-13 0.3898042102718904 1102 14-15 0.47915725263285935 1102 16-17 0.5219951775587859 1102 18-19 0.5265400392927901 1102 20-21 0.607068083164414 1102 22-23 0.5753223670052705 1102 24-25 0.6375133566313309 1102 26-27 0.690475680226065 1102 28-29 0.6968927451227742 1102 30-31 0.7367764517959117 1102 32-33 0.7361551429958553 1102 34-35 0.7510070885325746 1102 36-37 0.7709822742160952 1102 38-39 0.7716758513017439 1102 40-41 0.7681367243642967 1102 42-43 0.7849397337636148 1102 44-45 0.8000221566601411 1102 46-47 0.8184146114561273 1102 48-49 0.8253088516702007 1102 50-51 0.821461073567022 1102 52-53 0.7818569954170229 1102 54-55 0.7710151869516579 1102 56-57 0.7015882683006396 1102 58-59 0.7519621103050866 1102 60-61 0.7898767835703495 1102 62-63 0.72382466332148 1102 64-65 0.6894450086568042 1102 66-67 0.6764775854177145 1102 68-69 0.715824094005292 1102 70-71 0.7020736022351812 1102 72-73 0.7592952542846376 1102 74-75 0.6324168871471159 1102 76-77 0.6019588215523655 1102 78-79 0.5905316392283808 1102 80-81 0.6147294318237684 1102 82-83 0.6276000795859815 1102 84-85 0.588237485346518 1102 86-87 0.5746972866213014 1102 88-89 0.5178969801118711 1102 90-91 0.5581917679207713 1102 92-93 0.5211783515825772 1102 94-95 0.5816558932423845 1102 96-97 1.016928822663477 1102 98-99 0.7416583330000073 1102 100-101 0.7427264145422008 1103 1 0.3033968286069637 1103 2 0.2704986026554863 1103 3 0.2556717091509313 1103 4 0.2229881739881705 1103 5 0.191008525522399 1103 6 0.23495261099027687 1103 7 0.21782320410977007 1103 8 0.021112684774955426 1103 9 0.15426326892590936 1103 10-11 0.3123898153344058 1103 12-13 0.3243852458342147 1103 14-15 0.4026711912382197 1103 16-17 0.4053616040888741 1103 18-19 0.33879657725007917 1103 20-21 0.3240904118973802 1103 22-23 0.3818027057237501 1103 24-25 0.23651177544527258 1103 26-27 0.18092905484689226 1103 28-29 0.4037080374446802 1103 30-31 0.23635783946696876 1103 32-33 0.4288020781654751 1103 34-35 0.39835674048409686 1103 36-37 0.44782048333865987 1103 38-39 0.5072848531461815 1103 40-41 0.4058102063427853 1103 42-43 0.4738580845287501 1103 44-45 0.5454029932448492 1103 46-47 0.40210676687322433 1103 48-49 0.4033899832516141 1103 50-51 0.411548319969711 1103 52-53 0.4861472133114759 1103 54-55 0.5448344466852433 1103 56-57 0.48868650786720025 1103 58-59 0.25334096788198224 1103 60-61 0.19940910525865974 1103 62-63 0.08028476900449277 1103 64-65 0.31344907917024756 1103 66-67 0.2935992368593503 1103 68-69 0.2460567887983771 1103 70-71 0.23937557819170507 1103 72-73 0.389873921498598 1103 74-75 0.3355805051955443 1103 76-77 0.20165504256365097 1103 78-79 0.33007502030294944 1103 80-81 0.33607338628027605 1103 82-83 0.37249498849562457 1103 84-85 0.38236887284397625 1103 86-87 0.23125449385215546 1103 88-89 0.19342234970174843 1103 90-91 0.29858986098296114 1103 92-93 0.2610994199852392 1103 94-95 0.3191426233624135 1103 96-97 0.4832287510801585 1103 98-99 0.26079183573697406 1103 100-101 0.4850173067036927 1104 1 0.33462788563528534 1104 2 0.3348233717899589 1104 3 0.2973223345324456 1104 4 0.19741474125574143 1104 5 0.24758212931725865 1104 6 0.257755664897104 1104 7 0.23107564371956357 1104 8 0.3054810499258309 1104 9 0.2926934525173124 1104 10-11 0.3459679999002887 1104 12-13 0.3437661180206888 1104 14-15 0.4023270777003418 1104 16-17 0.4167779847036073 1104 18-19 0.4199549643387215 1104 20-21 0.3899973970509265 1104 22-23 0.5037553726510637 1104 24-25 0.44496543049790915 1104 26-27 0.541914396781074 1104 28-29 0.5425785339621285 1104 30-31 0.5668107185310305 1104 32-33 0.5953847933954322 1104 34-35 0.6168877832609283 1104 36-37 0.6418830704821801 1104 38-39 0.6154494899983192 1104 40-41 0.5573266960256191 1104 42-43 0.6429555774046705 1104 44-45 0.5841436168711311 1104 46-47 0.41592104362072035 1104 48-49 0.5339873175175001 1104 50-51 0.478165374069377 1104 52-53 0.5752841650353702 1104 54-55 0.6392635728268061 1104 56-57 0.6562378557774906 1104 58-59 0.6638276825160148 1104 60-61 0.5752369273855393 1104 62-63 0.3929823187971593 1104 64-65 0.5783503495300053 1104 66-67 0.6172714492646207 1104 68-69 0.6507669606347193 1104 70-71 0.5907153516800676 1104 72-73 0.6941757180110244 1104 74-75 0.6403253207064594 1104 76-77 0.5778565777542255 1104 78-79 0.5634729165100509 1104 80-81 0.700863908395398 1104 82-83 0.7113165787012505 1104 84-85 0.7539676241551021 1104 86-87 0.7290097324255171 1104 88-89 0.6432665089231904 1104 90-91 0.6808525003298946 1104 92-93 0.696534717384452 1104 94-95 0.7415461821747691 1104 96-97 1.0814832265634031 1104 98-99 0.8013056765168756 1104 100-101 0.8651605226135572 1105 1 0.15772848604479606 1105 2 0.12697735858830583 1105 3 0.11437546356251715 1105 4 0.044979802116849044 1105 5 0.09565717060778667 1105 6 0.05224323814536547 1105 7 0.0776925425747379 1105 8 0.1274936378803062 1105 9 0.15081602033558994 1105 10-11 0.1486329887857849 1105 12-13 0.1615081059615946 1105 14-15 0.13241351913566746 1105 16-17 0.11888818516063537 1105 18-19 0.08263598158459473 1105 20-21 0.15888302299834578 1105 22-23 0.17201725711111493 1105 24-25 0.19239661530978225 1105 26-27 0.1444063225033787 1105 28-29 0.17609185934845328 1105 30-31 0.13009741265872776 1105 32-33 0.22949981862224433 1105 34-35 0.17518799225886994 1105 36-37 0.1775815414600288 1105 38-39 0.18533008330926037 1105 40-41 0.2380015981727226 1105 42-43 0.1469767904128858 1105 44-45 0.30161350448170765 1105 46-47 0.25796777403318316 1105 48-49 0.26281372620321974 1105 50-51 0.14611328437278104 1105 52-53 0.25510479870354885 1105 54-55 0.3299447109747291 1105 56-57 0.3172627446324441 1105 58-59 0.2656251107666492 1105 60-61 0.2564466620514807 1105 62-63 0.2677326666557036 1105 64-65 0.27539208477853805 1105 66-67 0.3321807409058408 1105 68-69 0.3054903247096945 1105 70-71 0.3444586313639917 1105 72-73 0.3785017915647231 1105 74-75 0.36280214427713275 1105 76-77 0.3620332029324018 1105 78-79 0.3425830384867936 1105 80-81 0.3443124663378825 1105 82-83 0.32269484093568934 1105 84-85 0.3530678936747691 1105 86-87 0.2729860349153981 1105 88-89 0.28548257291785006 1105 90-91 0.3423317794142626 1105 92-93 0.31008836820235075 1105 94-95 0.35913165144443937 1105 96-97 0.7256213327705545 1105 98-99 0.534540396900816 1105 100-101 0.49627309374072937 1106 1 -0.06145826018865108 1106 2 -0.08567695291084476 1106 3 -0.08521839062122893 1106 4 -0.06709532176502364 1106 5 -0.05183501845781535 1106 6 -0.034902764842371425 1106 7 -0.029454149278208774 1106 8 -0.04405355337144101 1106 9 -0.004974944941046999 1106 10-11 -0.0580148308704409 1106 12-13 -0.07663504364398932 1106 14-15 -0.12836016188671806 1106 16-17 -0.12339003440177265 1106 18-19 -0.15196094972017704 1106 20-21 -0.16763403182393688 1106 22-23 -0.13993650409290836 1106 24-25 -0.059129448844636556 1106 26-27 -0.09661548662744934 1106 28-29 -0.12925252486298433 1106 30-31 -0.2559713275491404 1106 32-33 -0.26404648222852956 1106 34-35 -0.35205995708619753 1106 36-37 -0.22034735114075943 1106 38-39 -0.32474657714054445 1106 40-41 -0.3220100612978527 1106 42-43 -0.24940739312813065 1106 44-45 -0.18019308152510405 1106 46-47 -0.25802901391930533 1106 48-49 -0.27633029900127326 1106 50-51 -0.2798674945800528 1106 52-53 -0.13889100092662687 1106 54-55 -0.16174341868222797 1106 56-57 -0.16905528222988409 1106 58-59 -0.09539839775477787 1106 60-61 -0.21297970679768952 1106 62-63 -0.1914278173034134 1106 64-65 -0.13642373259133933 1106 66-67 -0.1400223097991926 1106 68-69 -0.13007691024230184 1106 70-71 -0.11276162958498048 1106 72-73 -0.06757617431311047 1106 74-75 -0.04391827664797887 1106 76-77 -0.0937083035304056 1106 78-79 -0.0848679126026397 1106 80-81 -0.17096511218137067 1106 82-83 -0.1606380654150783 1106 84-85 -0.11637521289096497 1106 86-87 -0.12949523193618262 1106 88-89 -0.17667655266740212 1106 90-91 -0.1523969927205151 1106 92-93 -0.09551321607376195 1106 94-95 -0.09983511052474014 1106 96-97 0.16919870371934564 1106 98-99 -0.018971121950176695 1106 100-101 0.006591881770642516 1107 1 -0.19070326929679737 1107 2 -0.2410130751428241 1107 3 -0.19815448400099456 1107 4 -0.15271037432891887 1107 5 -0.14399020154060338 1107 6 -0.1335569080138157 1107 7 -0.17162436103554057 1107 8 -0.1312023623306402 1107 9 -0.14418848121641048 1107 10-11 -0.2030096774514405 1107 12-13 -0.17373443163295121 1107 14-15 -0.2086622011126451 1107 16-17 -0.28131429050461776 1107 18-19 -0.30790268373349505 1107 20-21 -0.2563290443629498 1107 22-23 -0.18215696707747497 1107 24-25 -0.13314051025150064 1107 26-27 -0.16177201052860113 1107 28-29 -0.14491284844620367 1107 30-31 -0.23159313201022513 1107 32-33 -0.3087984063724747 1107 34-35 -0.31234021307217574 1107 36-37 -0.30270747607153226 1107 38-39 -0.2941931995087472 1107 40-41 -0.28971138739216684 1107 42-43 -0.3588997832468195 1107 44-45 -0.24669780138273723 1107 46-47 -0.27440153969674697 1107 48-49 -0.42463424225954327 1107 50-51 -0.3633468966687161 1107 52-53 -0.4035661981171117 1107 54-55 -0.3735251568792961 1107 56-57 -0.3766498480354912 1107 58-59 -0.2997668303251686 1107 60-61 -0.40334906354596 1107 62-63 -0.5142510486503227 1107 64-65 -0.44514490422938735 1107 66-67 -0.3697248140846696 1107 68-69 -0.37035910779339787 1107 70-71 -0.34834319393058166 1107 72-73 -0.3275375395686879 1107 74-75 -0.3430902742805628 1107 76-77 -0.4173057328555032 1107 78-79 -0.2391092295796895 1107 80-81 -0.27459315740017587 1107 82-83 -0.2855615045574851 1107 84-85 -0.28773147083416717 1107 86-87 -0.2733713572357104 1107 88-89 -0.2852440133612717 1107 90-91 -0.24972300485027077 1107 92-93 -0.2777132263641491 1107 94-95 -0.26244238966848243 1107 96-97 0.03210533342600996 1107 98-99 -0.2481050135879883 1107 100-101 -0.3144990118931794 1108 1 -0.34696906840706276 1108 2 -0.3527109050409507 1108 3 -0.34685431553481294 1108 4 -0.23324716784299682 1108 5 -0.2638748061127174 1108 6 -0.2622241253584505 1108 7 -0.30152079456655656 1108 8 -0.2580335768481632 1108 9 -0.3648814735350143 1108 10-11 -0.39658102213105906 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-0.17486081954938726 2305 18-19 -0.18633915970830373 2305 20-21 -0.15830036664542746 2305 22-23 -0.1789624671093435 2305 24-25 -0.10735016952077814 2305 26-27 -0.16590562817311394 2305 28-29 -0.17672330400926484 2305 30-31 -0.23038123473452998 2305 32-33 -0.26474740528431084 2305 34-35 -0.2144892141458925 2305 36-37 -0.14273782508383448 2305 38-39 -0.2031029292727382 2305 40-41 -0.2021640180802038 2305 42-43 -0.21039823657478252 2305 44-45 -0.22934938306889308 2305 46-47 -0.1183118339297522 2305 48-49 -0.17321038316536885 2305 50-51 -0.1729513516726371 2305 52-53 -0.23278360364165707 2305 54-55 -0.08509119219733918 2305 56-57 -0.1648535308852601 2305 58-59 -0.07979637827137509 2305 60-61 -0.10263768456361078 2305 62-63 -0.05015665633273869 2305 64-65 -0.06304528219713035 2305 66-67 -0.16448956909636792 2305 68-69 -0.07055605522923969 2305 70-71 -0.05499049955645319 2305 72-73 -0.05045645755176764 2305 74-75 -0.06961282350609821 2305 76-77 -0.10791948538412299 2305 78-79 -0.08913303954241059 2305 80-81 -0.08504046310677893 2305 82-83 -0.08609983801438403 2305 84-85 -0.05041300862450271 2305 86-87 -0.005168962550442302 2305 88-89 -0.01900112231822959 2305 90-91 -0.010641874302915966 2305 92-93 -0.031294554688706455 2305 94-95 -0.1371423991637606 2305 96-97 0.2243889005957378 2305 98-99 -0.048543420105165325 2305 100-101 -0.013296825189069494 2306 1 -0.25461185205389114 2306 2 -0.2943790701123845 2306 3 -0.22732060954642463 2306 4 -0.21900823926329593 2306 5 -0.21683302724478892 2306 6 -0.15586916986524813 2306 7 -0.17654405864374212 2306 8 -0.1661775679346249 2306 9 -0.32462922336087274 2306 10-11 -0.2507899097846078 2306 12-13 -0.304690696668402 2306 14-15 -0.3640799511261221 2306 16-17 -0.4276772931841606 2306 18-19 -0.3572850545777939 2306 20-21 -0.4416017120420648 2306 22-23 -0.37381296592543833 2306 24-25 -0.39123851106251806 2306 26-27 -0.440704592564785 2306 28-29 -0.5290626564254666 2306 30-31 -0.49099954188947237 2306 32-33 -0.597113765646057 2306 34-35 -0.6062725654079202 2306 36-37 -0.601344689840488 2306 38-39 -0.7632287182651183 2306 40-41 -0.6316569854923699 2306 42-43 -0.6776519532534095 2306 44-45 -0.7303548798227837 2306 46-47 -0.6472939056024885 2306 48-49 -0.6349560967549195 2306 50-51 -0.6405265771907338 2306 52-53 -0.5734927779040007 2306 54-55 -0.6528348010291296 2306 56-57 -0.6327581791822539 2306 58-59 -0.6552940856080625 2306 60-61 -0.6040761317113308 2306 62-63 -0.5590599953059296 2306 64-65 -0.6244687058777529 2306 66-67 -0.5868737854242383 2306 68-69 -0.5723433441586891 2306 70-71 -0.6544805689315822 2306 72-73 -0.6330070605284064 2306 74-75 -0.683822523957879 2306 76-77 -0.6445654330610822 2306 78-79 -0.5888327497566621 2306 80-81 -0.6111019005941998 2306 82-83 -0.586543871514543 2306 84-85 -0.530706347789824 2306 86-87 -0.6059375573454346 2306 88-89 -0.570640564674143 2306 90-91 -0.5723739865104562 2306 92-93 -0.6530229464918236 2306 94-95 -0.6507559820155713 2306 96-97 -0.4558461052497158 2306 98-99 -0.6880470495643749 2306 100-101 -0.6293511333976447 2307 1 -0.42888820480469647 2307 2 -0.4294701324558119 2307 3 -0.4325029893958323 2307 4 -0.31195641580577416 2307 5 -0.35731860101330426 2307 6 -0.3447312676333141 2307 7 -0.3389310469220632 2307 8 -0.3707981412195309 2307 9 -0.44518260671970467 2307 10-11 -0.5153371445783677 2307 12-13 -0.5000512476058319 2307 14-15 -0.8444470680950218 2307 16-17 -0.7213981462613361 2307 18-19 -0.6610789466568789 2307 20-21 -0.5495010900250961 2307 22-23 -0.5639667033521434 2307 24-25 -0.5300408473740319 2307 26-27 -0.6236234146162403 2307 28-29 -0.6276862192607453 2307 30-31 -0.6516129060650755 2307 32-33 -0.6859872690097149 2307 34-35 -0.678202709857203 2307 36-37 -0.7341558605728764 2307 38-39 -0.7315197446243715 2307 40-41 -0.65563991835689 2307 42-43 -0.7485070762752954 2307 44-45 -0.6785414412692816 2307 46-47 -0.6778855343613159 2307 48-49 -0.5830303355322854 2307 50-51 -0.6438208877023044 2307 52-53 -0.7347939472304361 2307 54-55 -0.756005613433743 2307 56-57 -0.8041495044283522 2307 58-59 -0.7784877194330875 2307 60-61 -0.8387116663871268 2307 62-63 -0.8522617431864603 2307 64-65 -0.8262576084776043 2307 66-67 -0.7875579591971018 2307 68-69 -0.7411281356295021 2307 70-71 -0.8632447945280077 2307 72-73 -1.1346302694322148 2307 74-75 -0.8553455022833845 2307 76-77 -0.8177105348550313 2307 78-79 -0.7392269616925162 2307 80-81 -0.7160984635716687 2307 82-83 -0.7093258411944134 2307 84-85 -0.7366245107376486 2307 86-87 -0.685437823475997 2307 88-89 -0.7229581595357928 2307 90-91 -0.8190012206177251 2307 92-93 -0.7771040459025862 2307 94-95 -0.7988389667877662 2307 96-97 -0.5878170268343403 2307 98-99 -0.9187830724814958 2307 100-101 -0.9641638696574262 2308 1 -1.3911926319187096 2308 2 -0.9078529731859319 2308 3 -0.8822606683164658 2308 4 -0.5759459958910398 2308 5 -0.5850831638050735 2308 6 -0.6134759571849742 2308 7 -0.609558123853148 2308 8 -0.6051663324025753 2308 9 -0.8365729041696923 2308 10-11 -0.8404656198422131 2308 12-13 -0.7551772342301533 2308 14-15 -1.0205349790564213 2308 16-17 -0.9310555058214973 2308 18-19 -0.910633802193793 2308 20-21 -0.9369856037990232 2308 22-23 -1.0116438003496668 2308 24-25 -1.1680908514816863 2308 26-27 -1.1268203086271527 2308 28-29 -1.18680107162497 2308 30-31 -1.0732873581472688 2308 32-33 -1.129737167849541 2308 34-35 -1.1711480276040405 2308 36-37 -1.2302361285605912 2308 38-39 -1.2542192482813803 2308 40-41 -1.1648651704270563 2308 42-43 -1.112803982528085 2308 44-45 -1.0900298422253272 2308 46-47 -1.0938797072857724 2308 48-49 -1.1688735716361194 2308 50-51 -1.2131899874695975 2308 52-53 -1.1801559521803355 2308 54-55 -1.1461302645452776 2308 56-57 -1.0962552383860569 2308 58-59 -1.0727228133744404 2308 60-61 -1.02680220148455 2308 62-63 -1.051675622132052 2308 64-65 -1.0480422141353145 2308 66-67 -1.0099247880879147 2308 68-69 -1.106823363297714 2308 70-71 -1.0645903124594227 2308 72-73 -1.11737133431064 2308 74-75 -1.2413182336178323 2308 76-77 -1.150970107511128 2308 78-79 -1.5538747637358732 2308 80-81 -1.2629729235663305 2308 82-83 -1.1476784201314771 2308 84-85 -1.0601093508102082 2308 86-87 -1.0505411613369589 2308 88-89 -1.0363633006469612 2308 90-91 -1.0846028586077914 2308 92-93 -0.9861116948537187 2308 94-95 -1.0943962433407215 2308 96-97 -0.9699569827896966 2308 98-99 -1.357294008561233 2308 100-101 -1.4287358385750757 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 38.0 3 73.0 4 144.0 5 397.0 6 753.0 7 1472.0 8 2941.0 9 4806.0 10 6554.0 11 8122.0 12 9739.0 13 10862.0 14 12193.0 15 13743.0 16 16091.0 17 19315.0 18 23009.0 19 26580.0 20 28487.0 21 28471.0 22 30425.0 23 35370.0 24 43102.0 25 54267.0 26 69674.0 27 89413.0 28 114500.0 29 144118.0 30 180360.0 31 223754.0 32 275096.0 33 347425.0 34 458444.0 35 669991.0 36 927228.0 37 1590468.0 38 2081127.0 39 714064.0 40 3815.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 17.99922616913068 31.779138313435862 28.039826882564995 22.18180863486846 2 22.58947301683482 29.80252378312414 28.048393603112153 19.55960959692889 3 21.26110821838791 28.63197124783691 28.567671334166956 21.539249199608225 4 21.380683915941116 28.948697836396903 28.76561926140485 20.90499898625713 5 21.864038019175318 28.946615527641868 28.739450459120132 20.44989599406268 6 21.749083733959672 28.76688863670428 28.337924794872176 21.14610283446387 7 21.871869492408514 28.82407171849617 28.670668151709002 20.633390637386313 8 21.125471354916808 28.86501353347692 28.670985103799403 21.33853000780687 9 20.803084316872496 28.767265319534385 29.243506608991186 21.18614375460193 10-11 20.932249963738887 29.188805906684518 28.60266419691884 21.276279932657758 12-13 20.9373791422199 29.293518569259213 28.384238615189556 21.384863673331335 14-15 21.15187920881454 29.22334318159796 28.230853194080975 21.393924415506525 16-17 21.35916334389049 28.987134834851947 28.215671309661932 21.43803051159563 18-19 21.348590461832924 29.0778753249135 28.21337890560025 21.36015530765333 20-21 21.413793933560928 28.96073892106521 28.314752763314665 21.3107143820592 22-23 21.294940017273493 28.942723281168277 28.321468969372056 21.44086773218617 24-25 21.312777895427875 28.856719904394325 28.33641007997001 21.494092120207792 26-27 21.570286767290927 28.738114070564414 28.445991018968854 21.245608143175804 28-29 21.51650452293136 28.78655250373468 28.36798008717426 21.328962886159697 30-31 21.46648898418193 28.794476116718325 28.338402631075006 21.40063226802474 32-33 21.50680868200557 28.793883357884436 28.283475661987623 21.41583229812237 34-35 21.51539763653746 28.8037788012747 28.2911815268282 21.38964203535964 36-37 21.522510742544153 28.82751939742798 28.225101013968423 21.424868846059443 38-39 21.542277435086557 28.814823470975565 28.215405173042633 21.427493920895245 40-41 21.540595935537358 28.84493924887294 28.214733782934857 21.39973103265484 42-43 21.555995568099462 28.852723744987408 28.187243079873287 21.404037607039847 44-45 21.593974473385188 28.855905287299926 28.15757489538109 21.392545343933797 46-47 21.56388893828546 28.87636151562869 28.141649038139917 21.41810050794593 48-49 21.591331252870795 28.86524426321347 28.098226429277638 21.44519805463809 50-51 21.63772975302159 28.87620425308092 28.088772530733035 21.397293463164456 52-53 21.615307742845733 28.942194037547765 28.014121209019947 21.42837701058655 54-55 21.65339552220299 28.926310520223296 27.965521033200424 21.454772924373287 56-57 21.632679205814455 28.93229254559797 27.95531711327416 21.479711135313416 58-59 21.657944038969177 29.01558725887871 27.89337986369208 21.433088838460034 60-61 21.641102411260007 28.985450179491135 27.911854277998042 21.461593131250815 62-63 21.654342408543094 29.001863624043807 27.894764124326848 21.449029843086254 64-65 21.63153328376723 29.063980919389117 27.898582720753236 21.40590307609042 66-67 21.683467277927175 29.060981752006054 27.87119157027614 21.384359399790632 68-69 21.62351466460789 29.110831693461687 27.863365898500664 21.402287743429756 70-71 21.664384843857693 29.15786675424995 27.822511989020636 21.35523641287172 72-73 21.651687398119503 29.11101231790042 27.805006396356347 21.43229388762373 74-75 21.658711592512457 29.161159447219127 27.805482226772355 21.374646733496064 76-77 21.65734120003854 29.18416131635065 27.786585875093028 21.37191160851778 78-79 21.638335761696617 29.198743000488697 27.763766399017154 21.39915483879753 80-81 21.645855770990668 29.227805061512097 27.720085217027968 21.406253950469264 82-83 21.670093457265565 29.3192804272028 27.647867143433324 21.362758972098312 84-85 21.625320772508395 29.286501283090033 27.715996904275347 21.37218104012622 86-87 21.614198488768714 29.358273241880273 27.654369808075746 21.373158461275267 88-89 21.626315025354277 29.373807141304425 27.649761211728507 21.35011662161279 90-91 21.63755170182916 29.374574451845515 27.64986564187806 21.338008204447263 92-93 21.63687105964844 29.41018012568418 27.58239733293951 21.37055148172787 94-95 21.607950402933774 29.450589443546882 27.616290054184724 21.32517009933462 96-97 21.59107877427184 29.473440481051092 27.574861335924755 21.360619408752314 98-99 21.607489599627897 29.48370958449238 27.5619091963345 21.34689161954523 100-101 21.645057778216266 29.508064254981353 27.509579262972522 21.33729870382986 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 94460.0 1 53240.0 2 9014.0 3 5404.0 4 4573.0 5 3954.5 6 3722.5 7 3795.5 8 3775.5 9 3436.5 10 3078.5 11 3694.0 12 7591.5 13 7705.5 14 4658.0 15 5264.0 16 6472.5 17 9284.0 18 14780.5 19 17659.5 20 20301.0 21 23954.0 22 28627.5 23 36855.0 24 45607.5 25 54357.0 26 66456.0 27 81237.0 28 93129.5 29 106477.5 30 122381.5 31 137368.0 32 154125.0 33 173211.5 34 192724.5 35 211159.0 36 230328.0 37 247823.0 38 260849.5 39 268550.0 40 274784.5 41 280768.5 42 282834.5 43 286591.0 44 292461.5 45 296462.5 46 298446.0 47 297840.0 48 298972.0 49 298181.5 50 288777.5 51 278884.0 52 270632.5 53 254051.0 54 229666.5 55 211216.0 56 193586.5 57 174663.5 58 160525.5 59 139747.5 60 113038.0 61 87121.5 62 62161.5 63 42224.0 64 28742.0 65 18992.0 66 12104.5 67 8737.5 68 5684.5 69 2663.5 70 1020.0 71 429.5 72 211.0 73 112.5 74 70.0 75 50.5 76 34.0 77 24.0 78 18.5 79 13.0 80 9.5 81 7.5 82 5.5 83 2.0 84 1.5 85 2.0 86 1.5 87 0.5 88 0.0 89 0.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.01281084908347992 2 0.018593271993681434 3 0.005794520029260512 4 4.233991670649643E-4 5 9.193810484839225E-4 6 0.0 7 0.0 8 0.007645379245287355 9 0.01056078493850611 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 1.1492263106049032E-4 24-25 0.004772313468775098 26-27 0.006834872268334425 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 7.258271435399388E-5 62-63 1.270197501194893E-4 64-65 3.5081645271097043E-4 66-67 1.875053454144842E-4 68-69 3.871078098879673E-4 70-71 1.6331110729648625E-4 72-73 7.258271435399388E-5 74-75 1.0887407153099082E-4 76-77 0.12646933120230483 78-79 0.05677782830341171 80-81 9.677695247199183E-5 82-83 0.0023347439783868032 84-85 0.010137385771441146 86-87 0.11998527538668137 88-89 0.13687285359304396 90-91 0.14965345987887638 92-93 0.14818970847273752 94-95 0.11481375698895932 96-97 1.203439549667807 98-99 0.07730059078700348 100-101 0.06954028891065564 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 8266431.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 74.17573151728001 #Duplication Level Percentage of deduplicated Percentage of total 1 88.7191306796246 65.80806417738314 2 6.833229692727727 10.137196221673554 3 1.7016633430757817 3.78666369806359 4 0.7559830538647873 2.2430238414035157 5 0.4351601181857999 1.6139160046788867 6 0.2631335339566492 1.1710873420776897 7 0.19573750078664262 1.0163280614349373 8 0.14708359061160128 0.872802634224293 9 0.11445562115740433 0.7640846483053624 >10 0.827667368945347 10.71372291333856 >50 0.005399654730253225 0.24543340873814612 >100 0.0010413858304676707 0.144437683975401 >500 8.984471528378654E-5 0.04628354596444973 >1k 4.49223575258184E-5 0.133985700471836 >5k 8.98447150516368E-5 0.5042540674559299 >10k+ 8.98447150516368E-5 0.798716050810721 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13852 0.16756929320525388 No Hit AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 10118 0.12239865063895167 No Hit AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT 8291 0.10029721411816055 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 1.209711905899898E-5 0.0 0.0 1.209711905899898E-5 0.0 6 1.209711905899898E-5 0.0 0.0 1.209711905899898E-5 0.0 7 1.209711905899898E-5 0.0 0.0 1.209711905899898E-5 0.0 8 1.209711905899898E-5 0.0 0.0 2.419423811799796E-5 0.0 9 1.209711905899898E-5 0.0 0.0 2.419423811799796E-5 0.0 10-11 1.209711905899898E-5 0.0 0.0 2.419423811799796E-5 0.0 12-13 1.209711905899898E-5 0.0 0.0 2.419423811799796E-5 0.0 14-15 1.209711905899898E-5 0.0 0.0 4.233991670649643E-5 0.0 16-17 1.8145678588498473E-5 0.0 0.0 4.838847623599592E-5 0.0 18-19 2.419423811799796E-5 0.0 0.0 4.838847623599592E-5 0.0 20-21 2.419423811799796E-5 0.0 0.0 4.838847623599592E-5 0.0 22-23 3.629135717699694E-5 0.0 0.0 4.838847623599592E-5 0.0 24-25 3.629135717699694E-5 0.0 0.0 4.838847623599592E-5 0.0 26-27 3.629135717699694E-5 0.0 0.0 4.838847623599592E-5 0.0 28-29 3.629135717699694E-5 0.0 0.0 6.04855952949949E-5 0.0 30-31 3.629135717699694E-5 0.0 0.0 6.653415482449438E-5 0.0 32-33 4.838847623599592E-5 0.0 0.0 7.258271435399388E-5 0.0 34-35 4.838847623599592E-5 0.0 0.0 7.863127388349337E-5 0.0 36-37 5.443703576549541E-5 0.0 0.0 8.467983341299287E-5 0.0 38-39 6.04855952949949E-5 0.0 0.0 8.467983341299287E-5 0.0 40-41 6.04855952949949E-5 0.0 0.0 8.467983341299287E-5 0.0 42-43 6.04855952949949E-5 0.0 0.0 1.0887407153099082E-4 6.04855952949949E-6 44-45 6.04855952949949E-5 0.0 0.0 1.0887407153099082E-4 1.209711905899898E-5 46-47 8.467983341299287E-5 0.0 0.0 1.1492263106049032E-4 1.209711905899898E-5 48-49 1.0282551200149133E-4 0.0 0.0 1.209711905899898E-4 1.209711905899898E-5 50-51 1.209711905899898E-4 0.0 0.0 1.330683096489888E-4 1.209711905899898E-5 52-53 1.5121398823748726E-4 0.0 0.0 1.3911686917848827E-4 1.209711905899898E-5 54-55 2.0565102400298265E-4 0.0 0.0 1.4516542870798775E-4 1.209711905899898E-5 56-57 2.479909407094791E-4 0.0 0.0 1.4516542870798775E-4 1.209711905899898E-5 58-59 3.4476789318147095E-4 0.0 0.0 1.4516542870798775E-4 1.209711905899898E-5 60-61 4.2339916706496427E-4 0.0 0.0 1.693596668259857E-4 1.209711905899898E-5 62-63 4.7178764330096023E-4 0.0 0.0 1.814567858849847E-4 1.209711905899898E-5 64-65 5.262246790664557E-4 0.0 0.0 1.814567858849847E-4 1.209711905899898E-5 66-67 5.988073934204496E-4 0.0 0.0 1.875053454144842E-4 1.209711905899898E-5 68-69 6.955843458924414E-4 0.0 0.0 1.935539049439837E-4 1.209711905899898E-5 70-71 7.863127388349337E-4 0.0 0.0 1.935539049439837E-4 1.8145678588498473E-5 72-73 9.61720965190419E-4 0.0 0.0 1.9960246447348317E-4 2.419423811799796E-5 74-75 0.001149226310604903 0.0 0.0 2.1774814306198164E-4 2.419423811799796E-5 76-77 0.0014274600489618797 0.0 0.0 2.1774814306198164E-4 2.419423811799796E-5 78-79 0.0016391596324943618 0.0 0.0 2.1774814306198164E-4 2.419423811799796E-5 80-81 0.00189924769226284 0.0 0.0 2.1774814306198164E-4 2.419423811799796E-5 82-83 0.0022500641449738103 0.0 0.0 2.2379670259148115E-4 2.419423811799796E-5 84-85 0.002891211455100756 0.0 0.0 2.2984526212098063E-4 2.419423811799796E-5 86-87 0.0035444558842867013 0.0 0.0 2.2984526212098063E-4 2.419423811799796E-5 88-89 0.004524322528065618 0.0 0.0 2.419423811799796E-4 2.419423811799796E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTCACG 280 1.6134436E-9 20.322577 3 CTCGCGT 130 1.5277692E-8 20.05669 52-53 TCAACGC 8180 0.0 19.182917 10-11 CAACGCA 8135 0.0 19.114204 12-13 ATCAACG 8195 0.0 19.032108 10-11 AACGCAG 8490 0.0 18.398724 12-13 AGTGGTA 9805 0.0 17.699465 4 ACTCGCG 150 7.87295E-8 17.382465 50-51 AAGCAGT 8785 0.0 17.059717 1 GGTATCA 10575 0.0 16.2759 7 ACGCAGA 9655 0.0 16.10503 14-15 TGGTATC 10635 0.0 16.094908 6 TATCAAC 11170 0.0 15.577773 9 GTATCAA 11255 0.0 15.418933 8 CGCAGAG 10280 0.0 15.218112 14-15 AGCAGTG 11890 0.0 15.03786 1 GTGGTAT 11760 0.0 14.918269 5 CGTACGC 200 1.3205499E-7 14.223609 20-21 CGCGTAG 185 8.5144165E-7 14.093891 54-55 AGAGTAC 10850 0.0 13.873105 18-19 >>END_MODULE