FastQCFastQC Report
Sat 18 Jun 2016
SRR578595_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR578595_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9093062
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT110278712.12778489798046No Hit
AAGCAGTGGTATCAACGCAGAGTATTTTTTTTTTTTTTTTTTTTTTTTTT223890.24622069001619035No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTG113780.12512836710010336No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACTAATCCTAGCCCTAGCCC95210.1047062034768926No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT2563950.091.30811
AGTGGTA2552150.091.270885
TGGTATC2556800.091.0332957
GGTATCA2561550.091.032358
GTATCAA2562050.090.9841169
GTGGTAT2564000.090.8653646
GCAGTGG2577400.090.5692753
AGCAGTG2587650.090.392772
CAGTGGT2582200.090.0047154
CAACGCA2554650.046.0959512-13
ATCAACG2554700.046.09369310-11
TCAACGC2550100.046.0375312-13
ACGCAGA2570150.045.8599214-15
AACGCAG2570550.045.7455914-15
CGCAGAG2573500.045.69026616-17
TATCAAC2566850.045.61913710-11
AGAGTAC2517700.045.5666518-19
CAGAGTA2564600.045.32785818-19
GCAGAGT2592950.045.2632716-17
GAGTACT1413600.045.15278220-21
Produced by FastQC (version 0.11.5)