FastQCFastQC Report
Sat 18 Jun 2016
SRR578595_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR578595_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9093062
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA893060.9821334111655677No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT134310.14770602026028196No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT92070.10125302125950533No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC82500.019.80468810-11
CGTCACG4650.019.4083233
CGCGTAG2250.018.99993754-55
ATCAACG87750.018.673929
AACGCAG92250.017.7887412-13
CAACGCA87850.017.51728212-13
ACGTACG3000.016.62403120-21
CGTACGC2451.8189894E-1216.4786320-21
CGCAGAG101200.016.28592714-15
AGTGGTA107850.015.5908773
GTATCAA108400.015.51100357
GGTATCA107300.015.4045926
ACGCAGA100500.015.28874114-15
TATCAAC111450.015.1717558
TGGTATC112900.015.0191375
CTCGCGT2709.094947E-1214.954652-53
ATCGGAA18300.014.2757512
AAGCAGT109100.014.2568041
ACTCGCG2902.910383E-1113.92336450-51
GTGGTAT123950.013.6424034
Produced by FastQC (version 0.11.5)