##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR578595_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 9093062 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.497067984359944 31.0 31.0 34.0 30.0 34.0 2 31.809320996601585 31.0 31.0 34.0 30.0 34.0 3 31.880156211406014 31.0 31.0 34.0 30.0 34.0 4 35.55525707401973 37.0 35.0 37.0 33.0 37.0 5 35.37853024646703 37.0 35.0 37.0 33.0 37.0 6 35.374805428578405 37.0 35.0 37.0 33.0 37.0 7 35.353857919367535 37.0 35.0 37.0 32.0 37.0 8 35.34059429046013 37.0 35.0 37.0 32.0 37.0 9 36.91073710923779 39.0 37.0 39.0 33.0 39.0 10-11 36.88193789946665 39.0 37.0 39.0 32.5 39.0 12-13 36.860445964186766 39.0 37.0 39.0 32.0 39.0 14-15 38.04730403245904 40.0 37.0 41.0 33.0 41.0 16-17 38.00167589311499 40.0 37.0 41.0 33.0 41.0 18-19 37.94970374116002 40.0 37.0 41.0 33.0 41.0 20-21 37.900744380715764 40.0 37.0 41.0 32.0 41.0 22-23 37.81351259894632 40.0 37.0 41.0 32.0 41.0 24-25 37.732957610978566 40.0 37.0 41.0 32.0 41.0 26-27 37.55230009429167 39.0 36.5 41.0 32.0 41.0 28-29 37.38090716856434 39.0 36.0 41.0 31.5 41.0 30-31 37.249579019696554 39.0 36.0 41.0 31.0 41.0 32-33 37.11929336894437 39.0 36.0 41.0 31.0 41.0 34-35 36.96291089844103 39.0 36.0 40.0 30.5 41.0 36-37 36.81293237635463 39.0 35.5 40.0 30.0 41.0 38-39 36.644222815152915 39.0 35.0 40.0 30.0 41.0 40-41 36.47528307846136 38.0 35.0 40.0 30.0 41.0 42-43 36.41543937564706 38.5 35.0 40.0 30.0 41.0 44-45 36.44872585274355 38.5 35.0 40.0 30.0 41.0 46-47 36.31760357512134 38.0 35.0 40.0 30.0 41.0 48-49 36.17058296754163 38.0 35.0 40.0 29.5 41.0 50-51 35.97110714740535 38.0 34.5 40.0 28.5 41.0 52-53 36.02969340800712 38.0 35.0 40.0 29.0 41.0 54-55 36.01534851516464 38.0 35.0 40.0 29.0 41.0 56-57 35.851358156361414 38.0 34.0 40.0 28.0 41.0 58-59 35.640791242817876 38.0 34.0 40.0 28.0 41.0 60-61 35.38176381069435 38.0 34.0 40.0 28.0 41.0 62-63 35.09828955306804 37.5 34.0 40.0 27.5 41.0 64-65 34.77483277910125 37.0 33.5 40.0 27.0 41.0 66-67 34.42822247335386 36.5 33.0 39.0 26.0 41.0 68-69 34.062319161576156 36.0 33.0 39.0 26.0 41.0 70-71 33.67626807119538 35.5 32.0 39.0 26.0 40.0 72-73 33.28393367382736 35.0 32.0 38.0 26.0 40.0 74-75 32.874547154742814 35.0 32.0 37.5 25.0 39.5 76-77 31.513447505361782 34.0 30.5 36.0 23.0 39.0 78-79 32.15562310033738 35.0 31.0 36.0 25.0 39.0 80-81 32.029321091179185 35.0 31.0 36.0 24.5 38.5 82-83 31.71751748750861 34.5 31.0 36.0 24.0 37.0 84-85 31.387122731594705 34.0 31.0 35.0 23.5 37.0 86-87 31.03743903868686 34.0 31.0 35.0 22.0 36.5 88-89 30.71564534586919 34.0 31.0 35.0 20.0 36.0 90-91 30.388771296181638 34.0 30.0 35.0 19.5 36.0 92-93 30.027724049390624 34.0 30.0 35.0 18.0 35.5 94-95 29.65592492385953 34.0 30.0 35.0 13.0 35.0 96-97 29.268228843045392 34.0 29.5 35.0 4.5 35.0 98-99 28.790597655663184 34.0 29.0 35.0 2.0 35.0 100-101 27.66486443180526 33.0 27.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2999455208115229 1101 2 0.3366815745470362 1101 3 0.3261443685390262 1101 4 0.27296556316063203 1101 5 0.2822009482234549 1101 6 0.2644276194010331 1101 7 0.29576002213612185 1101 8 0.2224977277646758 1101 9 0.29001456499262446 1101 10-11 0.3305456080383422 1101 12-13 0.3383558242962508 1101 14-15 0.4326360126372606 1101 16-17 0.4061115033511413 1101 18-19 0.34187504972338445 1101 20-21 0.37463987650036046 1101 22-23 0.4787172198759677 1101 24-25 0.48762221052328414 1101 26-27 0.37379764474772514 1101 28-29 0.5255434699133801 1101 30-31 0.5170236561304122 1101 32-33 0.5008446950818879 1101 34-35 0.4978076234707416 1101 36-37 0.5195306416066288 1101 38-39 0.4772978309103735 1101 40-41 0.46007830808186156 1101 42-43 0.5046514717799155 1101 44-45 0.5362662114828822 1101 46-47 0.47329292374343623 1101 48-49 0.3941655404707163 1101 50-51 0.3400444466871875 1101 52-53 0.4102669145896698 1101 54-55 0.4504166028412655 1101 56-57 0.5491793868105148 1101 58-59 0.447302198489929 1101 60-61 0.4652523987598798 1101 62-63 0.345872191437401 1101 64-65 0.3696292546108495 1101 66-67 0.3625371814492411 1101 68-69 0.422443553336322 1101 70-71 0.5087953513080663 1101 72-73 0.46645454281618015 1101 74-75 0.4468147658246906 1101 76-77 0.3159523244722031 1101 78-79 0.39178824500730514 1101 80-81 0.3965765623877644 1101 82-83 0.42371085203970793 1101 84-85 0.5247666320611231 1101 86-87 0.3846423351852728 1101 88-89 0.37804774123475227 1101 90-91 0.49863512257308074 1101 92-93 0.4301860975041514 1101 94-95 0.5881249088875009 1101 96-97 0.5215767682126469 1101 98-99 0.5327985473787216 1101 100-101 0.6095555448643672 1102 1 0.2918718676986529 1102 2 0.2499029275940572 1102 3 0.30792580182952634 1102 4 0.2341454070519262 1102 5 0.2543506673148741 1102 6 0.14492970154164908 1102 7 0.12331883454662318 1102 8 0.15190334853608078 1102 9 0.3634022097861731 1102 10-11 0.26658121102038024 1102 12-13 0.19739312617398497 1102 14-15 0.4254057185501523 1102 16-17 0.3749665363537744 1102 18-19 0.3916555460369153 1102 20-21 0.32644759971322657 1102 22-23 0.29920107767649995 1102 24-25 0.27108122326868056 1102 26-27 0.3293993378295852 1102 28-29 0.36225117578707966 1102 30-31 0.2999409359155223 1102 32-33 0.40807720209736686 1102 34-35 0.40978888550272075 1102 36-37 0.417935903524139 1102 38-39 0.4692624963366683 1102 40-41 0.365308418468544 1102 42-43 0.40145220587694297 1102 44-45 0.2739185235097352 1102 46-47 0.25281924493726393 1102 48-49 0.32040372746266144 1102 50-51 0.2989430459285245 1102 52-53 0.4379671889523138 1102 54-55 0.38331898970175615 1102 56-57 0.42842723019337114 1102 58-59 0.5514688973962265 1102 60-61 0.43591574778782416 1102 62-63 0.38658281809086503 1102 64-65 0.4607772030500641 1102 66-67 0.4682879811448899 1102 68-69 0.3485011706107528 1102 70-71 0.37730308192752204 1102 72-73 0.4260843369219458 1102 74-75 0.32029501434976027 1102 76-77 0.2531604818951898 1102 78-79 0.39002131276621554 1102 80-81 0.3777843722655305 1102 82-83 0.4222037275888262 1102 84-85 0.3679406301075083 1102 86-87 0.3199985132087093 1102 88-89 0.4091648948531912 1102 90-91 0.4521611031259276 1102 92-93 0.38584617904038154 1102 94-95 0.25881877585234747 1102 96-97 0.2504278986524078 1102 98-99 0.4186900088487562 1102 100-101 0.533425547333465 1103 1 0.18165040534522703 1103 2 0.21952752191754854 1103 3 0.26786973112488965 1103 4 0.09194519492556452 1103 5 0.17209187924027702 1103 6 0.08410186752496429 1103 7 0.12422036806029979 1103 8 0.08561267855673549 1103 9 0.2977315226639661 1103 10-11 0.2377480590397667 1103 12-13 0.29045634395372844 1103 14-15 0.3991826124641378 1103 16-17 0.29833874370076074 1103 18-19 0.29714139435029097 1103 20-21 0.25727014414771077 1103 22-23 0.30894154034280774 1103 24-25 0.2841065832994545 1103 26-27 0.2939048765312151 1103 28-29 0.2356472148245814 1103 30-31 0.2823987232102354 1103 32-33 0.29938448141675167 1103 34-35 0.3155086057437444 1103 36-37 0.3682908712769759 1103 38-39 0.30093395118262833 1103 40-41 0.3454792619146403 1103 42-43 0.3078266954984912 1103 44-45 0.26822706931121587 1103 46-47 0.34767894196902205 1103 48-49 0.3070736887253602 1103 50-51 0.24849151295910588 1103 52-53 0.2629168402854418 1103 54-55 0.2504416543855754 1103 56-57 0.2785913378162732 1103 58-59 0.22410072970141215 1103 60-61 0.22349324475951704 1103 62-63 0.21406391064436292 1103 64-65 0.2270394157289033 1103 66-67 0.1747193806450369 1103 68-69 0.20126641646428567 1103 70-71 0.10143723278724082 1103 72-73 0.22392011577207427 1103 74-75 0.22825246492414664 1103 76-77 0.20052452880666394 1103 78-79 0.2387828676710413 1103 80-81 0.30608760619499975 1103 82-83 0.2430821042457545 1103 84-85 0.3598453934964567 1103 86-87 0.4148194155634499 1103 88-89 0.3586994307459541 1103 90-91 0.2793306163372655 1103 92-93 0.24209684800063513 1103 94-95 0.27021813007563367 1103 96-97 0.2668593746557484 1103 98-99 0.41145538008252913 1103 100-101 0.5587074338720939 1104 1 0.15540097456864643 1104 2 0.1849186022819751 1104 3 0.20988111827338685 1104 4 0.15352767996630234 1104 5 0.16502711392147518 1104 6 0.22339585509061521 1104 7 0.16490872782598842 1104 8 0.13288569339228218 1104 9 0.2802065825954827 1104 10-11 0.2768235272346189 1104 12-13 0.20016469427937267 1104 14-15 0.26032790609962575 1104 16-17 0.2574557547308487 1104 18-19 0.30118379237342907 1104 20-21 0.35257321594750124 1104 22-23 0.34592027493950894 1104 24-25 0.32473099395411964 1104 26-27 0.33938131148735806 1104 28-29 0.3922426428600687 1104 30-31 0.298548587765346 1104 32-33 0.3204702843985956 1104 34-35 0.30053630385089036 1104 36-37 0.3598924531846066 1104 38-39 0.39326189660828703 1104 40-41 0.40379220023109497 1104 42-43 0.2845828899028433 1104 44-45 0.31637002953354454 1104 46-47 0.31662873099292455 1104 48-49 0.2248746379065807 1104 50-51 0.2899121154899973 1104 52-53 0.29236435423068485 1104 54-55 0.31433402541946975 1104 56-57 0.309180928604043 1104 58-59 0.36370949277192466 1104 60-61 0.3839327267981716 1104 62-63 0.31720202074220794 1104 64-65 0.3951749103589961 1104 66-67 0.43240267974608315 1104 68-69 0.4138932922638787 1104 70-71 0.3242679780135447 1104 72-73 0.3929762740494098 1104 74-75 0.42469461796621033 1104 76-77 0.38591366321830023 1104 78-79 0.465455207319323 1104 80-81 0.5422647005190555 1104 82-83 0.4318325362415045 1104 84-85 0.3394161460295777 1104 86-87 0.5276270383517634 1104 88-89 0.4684099319329462 1104 90-91 0.6018273911478111 1104 92-93 0.598067488132596 1104 94-95 0.5287608238082768 1104 96-97 0.5527231810974982 1104 98-99 0.5811012102290114 1104 100-101 0.6764664310794686 1105 1 0.15989018170298408 1105 2 0.14620382033267987 1105 3 0.09686263486563362 1105 4 0.11988870023879628 1105 5 0.13138731115675029 1105 6 0.14628381895150966 1105 7 0.1689630420932744 1105 8 0.1478164445220571 1105 9 0.15470808952724724 1105 10-11 0.09665528064923734 1105 12-13 0.13206916257149004 1105 14-15 0.17732514566571211 1105 16-17 0.14955407725918946 1105 18-19 0.16851763276353182 1105 20-21 0.19908966317763088 1105 22-23 0.1879094084120183 1105 24-25 0.2032797864153082 1105 26-27 0.19509090339685997 1105 28-29 0.1527755686780523 1105 30-31 0.1868980415994841 1105 32-33 0.16381928934824685 1105 34-35 0.23044194395047413 1105 36-37 0.18518902559343786 1105 38-39 0.24908014589158256 1105 40-41 0.2997114236711127 1105 42-43 0.18083723763810866 1105 44-45 0.14343359701076253 1105 46-47 0.19493155055054245 1105 48-49 0.22969049205280356 1105 50-51 0.24507765675203075 1105 52-53 0.11571144775868447 1105 54-55 0.2086743507173594 1105 56-57 0.221950160127939 1105 58-59 0.14011380249768735 1105 60-61 0.06300421044490889 1105 62-63 0.13456596289559286 1105 64-65 0.18304944538000711 1105 66-67 0.21207192874141612 1105 68-69 0.15200207520022957 1105 70-71 0.11978280046944434 1105 72-73 0.1808657883478233 1105 74-75 0.2685200956232663 1105 76-77 0.17234776787907435 1105 78-79 0.170034672007354 1105 80-81 0.17217477185103292 1105 82-83 0.26580999496396984 1105 84-85 0.27263558088811024 1105 86-87 0.2831902843671017 1105 88-89 0.3210647059751608 1105 90-91 0.37807097816525115 1105 92-93 0.25307813708196747 1105 94-95 0.3017802283592843 1105 96-97 0.3045405871956177 1105 98-99 0.5272702468642727 1105 100-101 0.579163975073854 1106 1 -0.04161926201788191 1106 2 0.004537473331001962 1106 3 -0.03853525286320547 1106 4 0.05281844741460162 1106 5 0.03591872744534186 1106 6 0.09105412292873183 1106 7 0.0784808126067631 1106 8 0.08711986988865306 1106 9 0.004954207276014699 1106 10-11 0.037081613482747855 1106 12-13 -0.021852046932174574 1106 14-15 0.1918673740290373 1106 16-17 0.16899107223957088 1106 18-19 0.010441391239375264 1106 20-21 0.07532017186721163 1106 22-23 0.053174563287441856 1106 24-25 0.2054752720566455 1106 26-27 0.2192112389022185 1106 28-29 0.16918510985753699 1106 30-31 0.1405999454623199 1106 32-33 0.06888852127620027 1106 34-35 0.05986033630566823 1106 36-37 0.10027586813612999 1106 38-39 0.08307078000555634 1106 40-41 0.04225028273232567 1106 42-43 0.057598434376373575 1106 44-45 0.05371069057296296 1106 46-47 0.07131948458521009 1106 48-49 0.1784005427904205 1106 50-51 0.1375872844100101 1106 52-53 0.01787634383465786 1106 54-55 -0.029690036937800812 1106 56-57 -0.007064391000248804 1106 58-59 0.13563175153772278 1106 60-61 0.2817633179985606 1106 62-63 0.16914528962305297 1106 64-65 0.14262702361174462 1106 66-67 0.10533339299345101 1106 68-69 0.13619903070424755 1106 70-71 0.09053842843380977 1106 72-73 0.1174101570421584 1106 74-75 0.09020727153183117 1106 76-77 -4.64727538194154E-5 1106 78-79 0.09980224232443646 1106 80-81 0.2312536870553643 1106 82-83 0.17989859386426588 1106 84-85 0.39406727766699845 1106 86-87 0.6453360279278542 1106 88-89 0.5251471326358939 1106 90-91 0.5658622611538782 1106 92-93 0.49431909555928755 1106 94-95 0.6467473134657666 1106 96-97 0.6452641554669292 1106 98-99 0.7255437423347786 1106 100-101 0.7444907319874119 1107 1 0.121540265386205 1107 2 0.12108992520839124 1107 3 0.1293168118181498 1107 4 0.15337562983716424 1107 5 0.029336611233752308 1107 6 0.029597855702682807 1107 7 0.0746605534218574 1107 8 0.05954707350868205 1107 9 -0.01918595859591221 1107 10-11 -0.049089383568123424 1107 12-13 -0.05596710206310007 1107 14-15 0.038389856557188295 1107 16-17 0.12389268542298026 1107 18-19 0.043107592523767835 1107 20-21 0.050868674340748044 1107 22-23 -0.095000365212492 1107 24-25 -0.04905901433689053 1107 26-27 0.025682057075833598 1107 28-29 -0.02886233966518148 1107 30-31 0.08758726224509417 1107 32-33 0.09931703710983442 1107 34-35 0.12256343359270261 1107 36-37 0.10008472538326174 1107 38-39 0.13485034802206997 1107 40-41 0.10817885989345655 1107 42-43 0.11074653561453829 1107 44-45 0.13148464544760685 1107 46-47 0.20289592773354315 1107 48-49 0.07697780991198755 1107 50-51 0.13263774492862979 1107 52-53 0.10461247316795408 1107 54-55 0.1256295333674089 1107 56-57 0.17529090045891138 1107 58-59 0.22847755908448164 1107 60-61 0.2842036329384783 1107 62-63 0.2921678349929522 1107 64-65 0.28258758365395664 1107 66-67 0.1744250625570416 1107 68-69 0.31721256969414924 1107 70-71 0.3040941184459527 1107 72-73 0.19510587880614594 1107 74-75 0.2149100819749421 1107 76-77 0.23209871526631076 1107 78-79 0.2723235787748379 1107 80-81 0.400513352841088 1107 82-83 0.4933295630138339 1107 84-85 0.46567363392065886 1107 86-87 0.3925493154209576 1107 88-89 0.363903943286239 1107 90-91 0.37250461242605937 1107 92-93 0.37771737618306744 1107 94-95 0.34460699486999147 1107 96-97 0.3587950657118206 1107 98-99 0.4153839512333022 1107 100-101 0.7274662963896041 1108 1 0.014514860729931911 1108 2 0.023227519959686305 1108 3 0.0029099241715435653 1108 4 0.028787523630867895 1108 5 -0.0734320654597056 1108 6 -0.02351578104371299 1108 7 0.02767241719957525 1108 8 0.00971379229829239 1108 9 -0.15195058696789232 1108 10-11 -0.181536189924266 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sequence quality scores pass #Quality Count 2 151.0 3 71.0 4 210.0 5 555.0 6 995.0 7 1976.0 8 3953.0 9 6144.0 10 8350.0 11 10675.0 12 13477.0 13 16264.0 14 18528.0 15 21309.0 16 24740.0 17 29120.0 18 34226.0 19 39440.0 20 41845.0 21 43614.0 22 47919.0 23 57169.0 24 70728.0 25 89372.0 26 112877.0 27 142510.0 28 180649.0 29 223433.0 30 272998.0 31 331836.0 32 405268.0 33 502536.0 34 642606.0 35 881547.0 36 1167663.0 37 1656888.0 38 1613571.0 39 376992.0 40 857.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 18.481413100412826 31.78644416044519 27.507314008755223 22.22482873038676 2 22.739732776483873 29.177421203110683 28.500718459854333 19.58212756055111 3 20.710526333153783 28.43098397437519 28.998064678322876 21.86042501414815 4 21.39830876619556 29.05625018558701 28.602021166267267 20.943419881950163 5 22.216916568807786 28.78431228504131 28.306792480303038 20.69197866584787 6 21.879632717089397 28.57085984108983 28.319257938250086 21.230249503570683 7 22.088697954550405 28.933630937521375 28.090152690039943 20.887518417888277 8 21.196034333914362 28.58763561181342 28.642292740059716 21.574037314212504 9 21.02106028810173 28.51818061246417 29.050730899837873 21.41002819959622 10-11 21.148371558497974 29.012943951699377 28.37226233222076 21.46642215758189 12-13 21.19750983744638 29.064745084191124 28.12209221955071 21.61565285881179 14-15 21.399867437378898 28.996022351549165 27.96108483160951 21.643025379462426 16-17 21.597264023783406 28.80387974941113 27.90670666859561 21.69214955820985 18-19 21.615839814557496 28.841855344421425 27.902083797744236 21.640221043276846 20-21 21.660740716790844 28.757605940444453 27.963615536557178 21.61803780620753 22-23 21.561307157858412 28.776082720841135 27.985340657762347 21.677269463538106 24-25 21.605290935000774 28.663721857389735 27.940148214099935 21.790838993509556 26-27 21.81258204401235 28.52488680517557 28.090858067989533 21.571673082822546 28-29 21.804757617623427 28.599552116054777 27.99581502802189 21.599875238299905 30-31 21.733531721930948 28.603804593866546 27.958561424957868 21.704102259244642 32-33 21.782744095547883 28.597582819592915 27.92258248279658 21.697090602062623 34-35 21.795937134782523 28.637179794966283 27.889421151845678 21.677461918405513 36-37 21.826646036962128 28.652577791116435 27.826302697327638 21.6944734745938 38-39 21.842268923874496 28.620507437359404 27.834617937312938 21.70260570145316 40-41 21.802259051299632 28.649430233191154 27.832796550660387 21.71551416484883 42-43 21.81631815123279 28.641079315604827 27.81530319707261 21.72729933608977 44-45 21.80368287207803 28.655614883165224 27.834885734231957 21.705816510524787 46-47 21.83244374352384 28.678821794236377 27.790197983796844 21.698536478442943 48-49 21.848036216811455 28.65985974646582 27.766229445543495 21.72587459117923 50-51 21.894521533183024 28.659754663309155 27.72789844666068 21.717825356847147 52-53 21.875136964285762 28.73420940983481 27.656999909538264 21.733653716341156 54-55 21.873399116955937 28.745530175071305 27.594698040326694 21.786372667646067 56-57 21.866105168973885 28.772194668858518 27.601054518268985 21.760645643898613 58-59 21.8525469081924 28.843844496917946 27.55071466909167 21.75289392579798 60-61 21.843155538783147 28.80496281938043 27.585494144849047 21.76638749698737 62-63 21.82608091746365 28.82091534003451 27.579012450426738 21.773991292075102 64-65 21.843613621309625 28.865304001961068 27.57346104158829 21.717621335141022 66-67 21.82369765045051 28.888836834186215 27.539710910542876 21.7477546048204 68-69 21.78558814839775 28.9308627758673 27.541994889135097 21.741554186599853 70-71 21.842060158807655 28.95406490490242 27.495489774797495 21.70838516149243 72-73 21.794189162757586 28.94025016770099 27.49629776591323 21.7692629036282 74-75 21.83531888136998 29.000343396113326 27.493398079126273 21.670939643390422 76-77 21.80167142817238 29.029033344323395 27.48843568866021 21.68085953884401 78-79 21.81772762574367 29.041350427391787 27.45503659823281 21.685885348631736 80-81 21.81571160046154 29.05309460453982 27.446472317545307 21.684721477453333 82-83 21.760954566872012 29.129442935263 27.440063947771954 21.669538550093034 84-85 21.766708123250655 29.152681163970666 27.414825855403585 21.665784857375094 86-87 21.787579183529825 29.191959572408937 27.387702357909554 21.63275888615168 88-89 21.770739115117085 29.24407510216327 27.346054548700828 21.63913123401882 90-91 21.75916277695853 29.24409653873804 27.329753075245794 21.666987609057635 92-93 21.786368144075624 29.273416564000726 27.300393901794262 21.639821390129388 94-95 21.753811532229165 29.35369339778343 27.29618880044324 21.59630626954416 96-97 21.72921258474757 29.365318299284187 27.255994046822707 21.64947506914553 98-99 21.734345372328924 29.379773282091335 27.236320394604153 21.64956095097559 100-101 21.787822604511287 29.399500344273505 27.14463809271707 21.668038958498133 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 97621.0 1 55482.0 2 9947.0 3 5927.0 4 4850.5 5 4417.0 6 4402.5 7 4181.5 8 3844.0 9 3542.0 10 3545.0 11 4366.0 12 7708.0 13 7546.5 14 4965.0 15 5478.0 16 6169.5 17 7378.0 18 9251.0 19 11505.5 20 14724.5 21 19268.0 22 25077.0 23 32553.0 24 40848.5 25 51239.0 26 66450.5 27 83563.5 28 97325.0 29 109605.0 30 127016.0 31 149646.0 32 173009.5 33 196933.5 34 220319.0 35 239772.0 36 256301.5 37 269991.5 38 280265.5 39 285607.5 40 292183.0 41 299730.0 42 304026.0 43 310580.5 44 318137.0 45 325378.0 46 327986.0 47 326041.0 48 323266.0 49 318676.0 50 312081.0 51 302664.0 52 297219.0 53 291801.5 54 277319.5 55 254363.5 56 226989.0 57 200851.0 58 177765.0 59 153879.0 60 127927.5 61 103943.5 62 80789.5 63 59706.5 64 43533.0 65 30027.0 66 19496.5 67 13331.0 68 8821.5 69 5604.5 70 3188.5 71 1445.5 72 665.5 73 306.5 74 164.5 75 95.0 76 65.5 77 48.5 78 35.5 79 26.0 80 17.5 81 11.0 82 12.0 83 8.0 84 4.0 85 3.5 86 5.0 87 4.5 88 1.5 89 2.0 90 1.5 91 0.5 92 0.0 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.14545155416294314 2 0.0 3 0.0 4 0.003211239514258233 5 3.9590624148389176E-4 6 0.0013856718451936212 7 0.0 8 1.3196874716129726E-4 9 2.639374943225945E-4 10-11 1.3196874716129726E-4 12-13 1.0997395596774771E-5 14-15 1.0997395596774771E-5 16-17 5.498697798387386E-6 18-19 1.0997395596774771E-5 20-21 5.4986977983873855E-5 22-23 2.309453075322702E-4 24-25 0.0 26-27 5.498697798387386E-6 28-29 0.00580662487509708 30-31 0.0014681523121694321 32-33 3.2442317010485577E-4 34-35 2.309453075322702E-4 36-37 2.7493488991936927E-4 38-39 0.0012866952848226483 40-41 0.003376200448209855 42-43 4.453945216693782E-4 44-45 5.498697798387385E-4 46-47 0.006329001165943881 48-49 0.006592938660266476 50-51 0.006801889176605197 52-53 0.009012365691556926 54-55 0.004662895733032503 56-57 0.0 58-59 8.248046697581079E-5 60-61 9.402773235242431E-4 62-63 8.85290345540369E-4 64-65 4.948828018548647E-4 66-67 6.98334620395198E-4 68-69 8.85290345540369E-4 70-71 0.0012372070046371617 72-73 0.0026558710366211074 74-75 7.093320159919728E-4 76-77 0.0 78-79 0.0 80-81 1.0997395596774771E-4 82-83 1.8145702734678374E-4 84-85 1.6496093395162157E-4 86-87 0.0028813176463549904 88-89 0.00155613147694363 90-91 5.388723842419638E-4 92-93 6.543450380080989E-4 94-95 3.574153568951801E-4 96-97 0.001127233048669414 98-99 0.0 100-101 1.0997395596774771E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 9093062.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.03508102930888 #Duplication Level Percentage of deduplicated Percentage of total 1 91.38519312715479 61.26013826157863 2 4.520289617145793 6.060359615226238 3 1.3531363908408802 2.7212282281117495 4 0.7035791477485459 1.8865794071942346 5 0.41404899543447826 1.38779039795271 6 0.2601594203292866 1.0463884693387053 7 0.170548675363616 0.8002920990708906 8 0.13294634007820968 0.7129654943754273 9 0.09325169580245293 0.5626021485814107 >10 0.8497574987422503 12.475345266016232 >50 0.09702540100598801 4.251623812918574 >100 0.019663807465751287 1.8209351450512254 >500 5.712612685635842E-5 0.02826137452517445 >1k 5.712612685635834E-5 0.14429384716918317 >5k 1.7137838056907498E-4 0.9069297193491945 >10k+ 1.1425225371271668E-4 3.934266713540438 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 89306 0.9821334111655677 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13431 0.14770602026028196 No Hit AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 9207 0.10125302125950533 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 2.1994791193549543E-5 0.0 0.0 0.0 0.0 2 2.1994791193549543E-5 0.0 0.0 0.0 0.0 3 6.598437358064863E-5 0.0 0.0 0.0 0.0 4 1.0997395596774772E-4 0.0 0.0 1.0997395596774771E-5 1.0997395596774771E-5 5 1.4296614275807203E-4 1.0997395596774771E-5 0.0 3.2992186790324316E-5 1.0997395596774771E-5 6 1.869557251451711E-4 1.0997395596774771E-5 0.0 3.2992186790324316E-5 2.1994791193549543E-5 7 1.9795312074194588E-4 1.0997395596774771E-5 0.0 5.498697798387386E-5 3.2992186790324316E-5 8 1.9795312074194588E-4 2.1994791193549543E-5 0.0 6.598437358064863E-5 3.2992186790324316E-5 9 2.1994791193549545E-4 3.2992186790324316E-5 0.0 6.598437358064863E-5 4.3989582387099085E-5 10-11 3.079270767096936E-4 4.3989582387099085E-5 0.0 8.797916477419817E-5 4.948828018548648E-5 12-13 3.629140546935675E-4 4.3989582387099085E-5 0.0 1.0997395596774772E-4 6.048567578226125E-5 14-15 4.1240233487905393E-4 5.498697798387386E-5 0.0 1.2647004936290986E-4 6.598437358064863E-5 16-17 4.343971260726035E-4 7.69817691774234E-5 0.0 1.4296614275807203E-4 6.598437358064863E-5 18-19 5.388723842419639E-4 7.69817691774234E-5 0.0 1.4296614275807203E-4 7.148307137903602E-5 20-21 5.993580600242251E-4 7.69817691774234E-5 0.0 1.4296614275807203E-4 7.69817691774234E-5 22-23 6.213528512177746E-4 7.69817691774234E-5 0.0 1.4296614275807203E-4 7.69817691774234E-5 24-25 6.70841131403261E-4 8.797916477419817E-5 0.0 1.539635383548468E-4 9.897656037097294E-5 26-27 7.533215983790719E-4 8.797916477419817E-5 0.0 1.539635383548468E-4 9.897656037097294E-5 28-29 7.753163895726214E-4 8.797916477419817E-5 0.0 1.539635383548468E-4 1.0447525816936033E-4 30-31 8.138072741613331E-4 9.347786257258556E-5 0.0 1.7045963175000894E-4 1.3746744495968466E-4 32-33 8.68794252145207E-4 9.897656037097294E-5 0.0 1.8145702734678374E-4 1.6496093395162157E-4 34-35 9.732695103145672E-4 1.0447525816936033E-4 0.0 2.0895051633872065E-4 1.7595832954839634E-4 36-37 0.001039253883895216 1.209713515645225E-4 0.0 2.3094530753227022E-4 1.8145702734678374E-4 38-39 0.0010942408618790898 1.209713515645225E-4 0.0 2.3094530753227022E-4 1.9795312074194588E-4 40-41 0.0011712226310565132 1.209713515645225E-4 0.0 2.7493488991936927E-4 2.0895051633872065E-4 42-43 0.0012866952848226483 1.209713515645225E-4 0.0 2.8593228551614407E-4 2.1994791193549545E-4 44-45 0.0013911705429920087 1.209713515645225E-4 0.0 2.9692968111291886E-4 2.5294009872581973E-4 46-47 0.0014791497077662067 1.209713515645225E-4 0.0 3.0242837891130623E-4 2.749348899193693E-4 48-49 0.0015891236637339545 1.209713515645225E-4 0.0 3.2442317010485577E-4 2.8593228551614407E-4 50-51 0.0017155937130968644 1.2647004936290986E-4 0.0 3.354205657016305E-4 2.9143098331453144E-4 52-53 0.0018860533448468732 1.3196874716129726E-4 0.0 3.4091926350001794E-4 3.2992186790324314E-4 54-55 0.0020290194876049455 1.3196874716129726E-4 0.0 3.4091926350001794E-4 3.2992186790324314E-4 56-57 0.002182983025959792 1.3746744495968466E-4 0.0 3.4091926350001794E-4 3.2992186790324314E-4 58-59 0.0023479439599114137 1.4296614275807203E-4 0.0 3.6841275249195485E-4 3.2992186790324314E-4 60-61 0.0025074061960646482 1.484648405564594E-4 0.0 3.84908845887117E-4 3.2992186790324314E-4 62-63 0.0027108580146049813 1.539635383548468E-4 0.0 4.014049392822792E-4 3.4091926350001794E-4 64-65 0.0029363046243388643 1.539635383548468E-4 0.0 4.1240233487905393E-4 3.519166590967927E-4 66-67 0.0031837460252662963 1.7595832954839634E-4 0.0 4.2339973047582867E-4 3.574153568951801E-4 68-69 0.0033157147724275937 1.7595832954839634E-4 0.0 4.288984282742161E-4 3.6841275249195485E-4 70-71 0.0035906496623469633 1.7595832954839634E-4 0.0 4.343971260726035E-4 3.8490884588711697E-4 72-73 0.003832592365476008 1.869557251451711E-4 0.0 4.398958238709909E-4 4.014049392822792E-4 74-75 0.004036044184016341 1.869557251451711E-4 0.0 4.618906150645404E-4 4.0690363708066656E-4 76-77 0.004244994700355062 1.869557251451711E-4 0.0 4.783867084597026E-4 4.0690363708066656E-4 78-79 0.004530926985871206 1.9795312074194588E-4 0.0 4.8388540625809E-4 4.343971260726035E-4 80-81 0.005058801974516395 1.9795312074194588E-4 0.0 5.003814996532522E-4 4.398958238709909E-4 82-83 0.005740640501516431 1.9795312074194588E-4 0.0 5.168775930484143E-4 4.398958238709909E-4 84-85 0.006526954286685828 1.9795312074194588E-4 0.0 5.168775930484143E-4 4.398958238709909E-4 86-87 0.007775158686919763 2.1444921413710805E-4 0.0 5.168775930484143E-4 4.398958238709909E-4 88-89 0.009567734169194052 2.1994791193549545E-4 0.0 5.388723842419638E-4 4.398958238709909E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCAACGC 8250 0.0 19.804688 10-11 CGTCACG 465 0.0 19.408323 3 CGCGTAG 225 0.0 18.999937 54-55 ATCAACG 8775 0.0 18.67392 9 AACGCAG 9225 0.0 17.78874 12-13 CAACGCA 8785 0.0 17.517282 12-13 ACGTACG 300 0.0 16.624031 20-21 CGTACGC 245 1.8189894E-12 16.47863 20-21 CGCAGAG 10120 0.0 16.285927 14-15 AGTGGTA 10785 0.0 15.590877 3 GTATCAA 10840 0.0 15.5110035 7 GGTATCA 10730 0.0 15.404592 6 ACGCAGA 10050 0.0 15.288741 14-15 TATCAAC 11145 0.0 15.171755 8 TGGTATC 11290 0.0 15.019137 5 CTCGCGT 270 9.094947E-12 14.9546 52-53 ATCGGAA 1830 0.0 14.275751 2 AAGCAGT 10910 0.0 14.256804 1 ACTCGCG 290 2.910383E-11 13.923364 50-51 GTGGTAT 12395 0.0 13.642403 4 >>END_MODULE