FastQCFastQC Report
Sat 18 Jun 2016
SRR578594_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR578594_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9183687
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT112000212.195559365209203No Hit
AAGCAGTGGTATCAACGCAGAGTATTTTTTTTTTTTTTTTTTTTTTTTTT229350.2497362987218532No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTG115180.12541803743964705No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACTAATCCTAGCCCTAGCCC97030.1056547332242486No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT2582900.091.367631
AGTGGTA2571650.091.257875
TGGTATC2575700.091.071597
GGTATCA2586600.091.001348
GTATCAA2592500.090.952089
GTGGTAT2582800.090.931286
AGCAGTG2603800.090.4749452
GCAGTGG2600100.090.459983
CAGTGGT2612050.089.778314
CAACGCA2579600.046.08649412-13
ATCAACG2581000.046.0854310-11
TCAACGC2578700.045.98557312-13
ACGCAGA2591550.045.8741514-15
AACGCAG2592850.045.74669614-15
CGCAGAG2594750.045.64510316-17
TATCAAC2596500.045.61633310-11
AGAGTAC2534900.045.5857918-19
CAGAGTA2579150.045.32030518-19
GAGTACT1419950.045.23176620-21
GCAGAGT2613000.045.22813416-17
Produced by FastQC (version 0.11.5)